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All Journal Hemera Zoa Jurnal Sain Veteriner Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati
Teguh Budipitojo
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Veterinary

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PCS-10 The Features of Seminiferous Tubule Cells in Rat Testicular Dysfunction Induce by Secretome Based On Cytoskeletal Protein Profile Surya Agus Prihatno; Irma Padeta; Arinda Devi Larasati; Betty Sundari; Annisa Hidayati; Yuda Heru Fibrianto; Teguh Budipitojo
Hemera Zoa Proceedings of the 20th FAVA & the 15th KIVNAS PDHI 2018
Publisher : Hemera Zoa

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Abstract

Secretome is a factor found in the stem cell culture medium. This factor may repair the tissues of the organs that were damaged by various degenerative disorders1. A previous study has reported that secretome derived from the fetal human umbilical cord mesenchymal stem cell (HUC-MSC) may be an effective regenerative agent for β-cell pancreatic regeneration in Type 1 diabetes mellitus and for skin regeneration in incisional and burn wound healing.The administration of cisplatin as a chemotherapeutic agent is limited because it causes side effects, such as reproductive toxicity and progresses to testicular dysfunction2. As a degenerative disorder, testicular dysfunction is characterized by failure in the synthesis of reproductive hormones and spermatogenesis. Cisplatin-induced testicular cell damage would promote vimentin and cytokeratin localization in the spermatogenic, Sertoli, and Leydig cells.Studies on the use of secretome in promoting the recovery of various degenerative disorders and its effects on the spermatogenesis of rats with cisplatin-induced testicular dysfunction have not been conducted. The aim of this stud was to investigate the effect of secretome derived from HUC-MSC on cisplatin-induced testicular dysfunction in rats.
PCS-11 Mesenchymal Stem Cell-Conditioned Medium Improve the Recovery of Pancreatic α and β Cells in Type 1 Diabetes Mellitus Dian Bekti Hadi Masithoh; Yuda Heru Fibrianto; Marla Anggita; Widagdo Sri Nugroho; Teguh Budipitojo
Hemera Zoa Proceedings of the 20th FAVA & the 15th KIVNAS PDHI 2018
Publisher : Hemera Zoa

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Abstract

Mesenchymal stem-cell is a pluripotent cells that can be differentiated into many kinds of cells (1) and have abilities to differentiated into mature cell, secreted cytokines, and releasing many kinds of protein functioned in body body regulation (2). However, the therapeutic effect of stem cell arise from their secreted factor such as growth factors, cytokines, chemokines, and metabolits which acts as biologic regulator in the autocrine and paracrine body function (3). Secreted factors of mesenchymal stem cells known as secretome or mesenchimal stem cell conditioned medium (MSC-CM) have therapeutic effect for antiapoptosis, angiogenic, immunomodulatoric, and chemoattraactive activity (4). Mesenchymal stem cell conditioned medium used as the replacement agent for cell therapy because it contains no cells and save for used in different individual (5).Type 1 diabetes mellitus (DMT1) is a disorder characterized by destruction of the insulin-producing pancreatic β-cell that progressively leads to insulin deficiency and resultant hyperglycemia. Insulin deficiency can lead to progressive metabolic derangement, with worsening hyperglycemia, ketoacidosis, starvation, and death (6). Glucagon is a hormone produced by pancreatic α cells. Glucagon releases by the pancreas to raise the concentration of glucose in the bloodstream when the concentration of glucose in the bloodstream too low (7). Glucagon worked in opposite with insulin, which lowers the extracellular glucose level, while insulin works to increase the extracellular glucose level (8). The aim of this study was to investigate the role of MSC-CM on the structural and functional regeneration of pancreatic α and β cells in Wistar rat (Rattus norvegicus) induced with type 1 diabetes mellitus. The datas in this study will completes the information about the effect of MSC-CM on pancreatic cells regeneration, which has been presented in previous study (9).
PCS-12 Complex Oligosaccharide Detected in the Lingua of Hystrix javanica by Lectin Histochemistry Teguh Budipitojo; Yosua Kristian Adi; Yuda Heru Fibrianto; . Ariana
Hemera Zoa Proceedings of the 20th FAVA & the 15th KIVNAS PDHI 2018
Publisher : Hemera Zoa

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Hystrix javanica or usually called Sunda porcupine is wildlife animal that only found in Java, Bali, Sumbawa, Flores, Lombok, Madura, and Tonahdjampea (Indonesia) [1]. International Union for Conservation of Nature and Natural Resources (IUCN) has classified this Rodentia into Least Concern criteria, that means the species is relatively widespread and abundant [2]. Nevertheless, their population in nature was threatened, since this mammal was considered as pest by some people.There were anatomical data of Sunda porcupine organ, but it still limited. One of the ways to determine the detail function of the organ can be done by making histological preparations. Lectin histochemistry has been used widely as a probe to detect sugar residues in the organs or tissues because lectin can bind specifically to carbohydrate residues in term of glycoconjugates [3]. Analysis the composition or type of sugar residues in the organs can help to understanding the role of their function.The aims of this study were to detect and find out the distribution of complex oligosaccharide in the lingua of Hystrix javanica using lectin histochemistry method. This data will completes the information that has been obtained before about mucopolysaccharide type of major salivary glands of Hystrix javanica [4].
PCS-13 The Profile and Morphology of Proboscis Monkey’s Blood Cells in South Kalimantan Irma Padeta; Rini Widayanti; Heri Budi Santoso; Amalia Rezeki; Teguh Budipitojo
Hemera Zoa Proceedings of the 20th FAVA & the 15th KIVNAS PDHI 2018
Publisher : Hemera Zoa

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Abstract

Twenty percent of primate species is found in Indonesia1. Proboscis monkey (Nasalis larvtaus) or bekantan is one of endemic species in Kalimantan2,3. This primate is belonging to Colobinae subfamily and distributed in Kalimantan (Indonesia), Sabah and Serawak (Malaysia), and Brunei. Proboscis monkey is belonging to endangered species based on International Union for the Conservation of Nature and Natural Resources (IUCN) assessment4. Indonesian Ministry of environment and forestry established Proboscis monkey is belonging to one of twenty five species which has been incrased in population.Blood cells have important role in body regulation such as maintaining body temperature, carriying oxygen, nutrition, hormone and metabolism product to the whole body5. Blood examination is important for body health evaluation6,7, especially in non-human primate where lived in zoo, animal captive or rehabilitiation center6. The aim of this study was to investigate the profile and morphology of female-juvenile proboscis monkey blood cells at rehabilitation center (Sahabat Bekantan Indonesia Foundation) in Banjarmasin, South Kalimantan.
Distribusi Epitelium Olfaktorius Pada Hidung Kalong Kapauk (Pteropus Vampyrus) dan Lasiwen Deignan (Myotis Horsfielddii) Teguh Budipitojo; Yosephin Nicolory Paula
Jurnal Sain Veteriner Vol 27, No 2 (2009): DESEMBER
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (2065.119 KB) | DOI: 10.22146/jsv.461

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Morfologi dan Morfometri Struktur Anatomis Lambung Codot (Rosettus sp) dan Lawa (Myotis sp) Ariana .; Teguh Budipitojo
Jurnal Sain Veteriner Vol 27, No 2 (2009): DESEMBER
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jsv.464

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Morfometri, Morfologi Serta Abnormalitas Spermatozoa Owa Jawa (Hylobates moloch) Asal Ejakulat Pudji Astuti; Tuty Laswardi Yusuf; Dondin Sajuthi; Eric Hayes; Teguh Budipitojo; Luthfiralda Sjahfirji; Hera Maheshwari
Jurnal Sain Veteriner Vol 22, No 2 (2004): DESEMBER
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1474.497 KB) | DOI: 10.22146/jsv.486

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Identifikasi Immunohistokimiawi Desmin dan Vimentin dalam Sel Otot Skelet Ayam Kedu Cemani (Galllus gallus domesticus) Teguh Budipitojo; Hevi Wihadmadyatami; Ariana -; Dewi K. Musana
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 19, No 1 (2014): February 2014
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24002/biota.v19i1.451

Abstract

AbstractThe two sub units of intermediate filaments, desmin and vimentin, are found in most cells of mesenchymal origin. The content and distribution of desmin in mature skeletal muscle have been known, but for vimentin has been a subject of disagreement. The present study was aimed to clarify the presence of desmin and vimentin immunoreactivities in the skeletal muscle fibers of Kedu Cemani chickens (Gallus gallus domesticus) by immunohistochemistry methods of avidin-biotin-complexs. Samples of muscle tissues were obtained from pectorales mayor, biceps brachii, and biceps femoris of five adult Kedu Cemani chickens (Gallus gallus domesticus). Immunohistochemical staining results showed that desmin immunoreactivities were detected in Z disc of myofibers, but not vimentin. The results stimulate further exploration on characteristics of Kedu Cemani chickens, especially in terms of the distribution of other intermediate filaments and neuroendocrine cells in a variety of organs system.Key words: Kedu chicken, skeletal muscle, desmin, vimentin, immunohistochemistryAbstrakVimentin dan desmin merupakan subunit filamen intermedia dan dapat ditemukan dalam sebagian besar sel mesenkimal. Keberadaan dan distribusi desmin dalam sel otot skelet dewasa/masak telah diketahui, namun keberadaan dan distribusi vimentin pada sel yang sama masih menjadi perdebatan. Penelitian ini bertujuan mengklarifikasi keberadaan immunoreaktifitas desmin dan vimentin dalam serabut otot skelet ayam kedu cemani dengan metode immunohistokimia komplek avidin-biotin. Penelitian dilakukan menggunakan jaringan otot yang meliputi otot pectorales mayor, biceps brachii, dan biceps femoris dari 5 ekor ayam kedu cemani (Gallus gallus domesticus). Pengamatan terhadap immunoreaktifitas desmin dan vimentin dalam serabut otot normal ayam kedu cemani dewasa dengan menggunakan antibodi monoklonal terhadap desmin dan antibodi poliklonal terhadap vimentin memperoleh hasil bahwa immunoreaktifitas desmin terdeteksi pada diskus Z serabut otot skelet, namun immunoreaktifitas vimentin tidak terdeteksi. Hasil penelitian memunculkan dorongan untuk menggali lebih lanjut ciri-ciri khusus ayam kedu cemani, terutama dalam hal distribusi filamen intermedia dan jenis neuroendokrin lainnya pada berbagai sistem organ tubuh.Kata kunci: Ayam kedu, otot skelet, desmin, vimentin, immunohistokimia
Co-Authors . Ariana Amalia Rezeki Annisa Hidayati Ariana - Ariana . Arinda Devi Larasati Betty Sundari Dewi K. Musana Dian Bekti Hadi Masithoh Dondin Sajuthi ERIC HAYES HERA MAHESHWARI Heri Budi Santoso Hevi Wihadmadyatami Irma Padeta Luthfiralda Sjahfirji Marla Anggita PUDJI ASTUTI Rini Widayanti Surya Agus Prihatno Tuty Laswardi Yusuf Widagdo Sri Nugroho Yosephin Nicolory Paula Yosua Kristian Adi Yuda Heru Fibrianto