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All Journal Hemera Zoa Jurnal Sain Veteriner Jurnal Veteriner Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati
Rini Widayanti
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Veterinary

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PCS-13 The Profile and Morphology of Proboscis Monkey’s Blood Cells in South Kalimantan Irma Padeta; Rini Widayanti; Heri Budi Santoso; Amalia Rezeki; Teguh Budipitojo
Hemera Zoa Proceedings of the 20th FAVA & the 15th KIVNAS PDHI 2018
Publisher : Hemera Zoa

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Abstract

Twenty percent of primate species is found in Indonesia1. Proboscis monkey (Nasalis larvtaus) or bekantan is one of endemic species in Kalimantan2,3. This primate is belonging to Colobinae subfamily and distributed in Kalimantan (Indonesia), Sabah and Serawak (Malaysia), and Brunei. Proboscis monkey is belonging to endangered species based on International Union for the Conservation of Nature and Natural Resources (IUCN) assessment4. Indonesian Ministry of environment and forestry established Proboscis monkey is belonging to one of twenty five species which has been incrased in population.Blood cells have important role in body regulation such as maintaining body temperature, carriying oxygen, nutrition, hormone and metabolism product to the whole body5. Blood examination is important for body health evaluation6,7, especially in non-human primate where lived in zoo, animal captive or rehabilitiation center6. The aim of this study was to investigate the profile and morphology of female-juvenile proboscis monkey blood cells at rehabilitation center (Sahabat Bekantan Indonesia Foundation) in Banjarmasin, South Kalimantan.
Kajian Penanda Genetik Gen Cytochrome B Pada Tarsius sp. =Study of Genetic Marker on Cytochrome B Gene of Tarsius sp. Rini Widayanti; Dedy Duryadi Solihin; Dondin Sajuthi; RR. Dyah Perwitasari
Jurnal Sain Veteriner Vol 24, No 1 (2006): JUNI
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1938.625 KB) | DOI: 10.22146/jsv.349

Abstract

Tarsius merupakan salah satu satwa endemik Indonesia yang keberadaannya mulai memprihatinkan. Konservasi sebagai salah satu cara untuk pelestarian satwa ini akan lebih terarah dan berhasil guna apabila karakteristik dan keragaman sumber genetiknya diketahui dengan pasti. Tujuan dari penelitian ini adalah mengkaji penanda genetik spesifik gen cyt b pada Tarsius sp. Pengurutan hasil PCR menggunakan primer H 15149 pada gen cyt b didapatkan urutan basa sebesar 276 pb (menyandi 92 asam amino. Fragmen cyt b hash! pengurutan disejajarkan berganda dengan primata lain dari data Genbank dengan bantuan perangkat lunak Genetyx-Win versi 3.0 dan Clustal W, kemudian dianalisis dengan menggunakan program MEGA versi 3.1. Dari hasil analisis diperoleh 14 situs asam amino yang berbeda. Tarsius dianae memiliki 12 situs asam amino (asam amino ke 2, 6, 9, 22, 23, 29, 39, 41, 42, 45, 55 dan 85), T. spectrum memiliki 7 situs asam amino (asam amino ke 2, 6, 9, 41, 45, 55 dan 85) dan T bancanus memiliki 2 situs asam amino ( ke 23 dan 45) yang dapat digunakan sebagai penanda genetik. Lima asam amino unik ditemukan pada T dianae, yaitu pada situs asam amino ke 6 (valina), ke 22 (alanina), ke 29 (alanina), ke 39 (serina) dan ke 42 (valina). Jarak genetik berdasar nukleotida cyt b yang dihitung menggunakan model 2 parameter Kimura ditemukan nilai paling kecil sebesar 0,7%, nilai paling besar 22,3% dan rata-rata 13,1%. Filogram menggunakan metode neighbor joining berdasar hasil urutan nukleotida dan asam amino cyt b tersebut dapat dijadikan pembeda masing-masing spesies Tarsius.
KARAKTERISASI ANTIBODI MONOKLONAL TERHADAP PROTEIN MEMBRAN Toxoplasma gondii ISOLAT LOKAL Rini Widayanti; Widya Asmara; Wayan Tunas Artama
Jurnal Sain Veteriner Vol 22, No 1 (2004): Juli
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1255.568 KB) | DOI: 10.22146/jsv.443

Abstract

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Kajian Molekuler Daerah D-Loop Parsial Deoxyribonucleic acid (DNA) Mitokondria Kuda (Equus caballus) Asli Priangan Yuriadi .; Rini Widayanti; Aris Haryanto; Wayan Tunas Artama
Jurnal Sain Veteriner Vol 27, No 2 (2009): DESEMBER
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1948.518 KB) | DOI: 10.22146/jsv.463

Abstract

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Kajian Molekuler Daerah D-Loop Parsial DNA Mitokondria Kuda (Equus caballus) Asli Tengger Yuriadi -; Rini Widayanti; Aris Purwantoro; Charles Rangga Tabbu
Jurnal Veteriner Vol 11 No 1 (2010)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

Tengger’s horse (Equus caballus) is a local Indonesian horse an originated from its ancestor in Java.As the population of Tengger’s horse is almost extinct it is important to conserve and increase the horsepopulation by in situ or ex situ conservation.The objective of this research was to study the moleculargenetic of partial D-loop of Tengger’s horse. Sequencing of PCR product, showed that the D-loop consistedof 319 nucleotides. The DNA was isolated from whole blood and amplified and sequenced using a publishedprimer sets. The sequence was aligned and compared with horse D-loop sequences available in Genebankusing Clustal W method in MEGA program version 4.0.2. Ten different nucleotide sites were found inTengger horse from (nucleotide no. 9, 52, 64, 69, 102, 117, 133, 170, 187 and 293). The genetic distanceanalised using Kimura 2-parameter model ranged between 0,0% and 3,2%, with the average of 1,7%. Thephylogenetic tree using neighbor joining method based on the sequence of nucleotide partial D-loop couldnot be used to differentiate among horse from Tengger and E. caballus.
Kekerabatan Genetik Caplak Rhiphicephalus (Boophilus) microplus Asal IndonesiaBerdasarkan Sekuen Internal Transcribed Spacer-2 (GENETIC RELATIONSHIP INDONESIAN RHIPHICEPHALUS (BOOPHILUS) MICROPLUS TICK BASED ON INTERNAL TRANSCRIBED SPACER-2 SEQUENSE ) Ana Sahara; Joko Prastowo; Rini Widayanti; Kurniasih .; Wisnu Nurcahyo
Jurnal Veteriner Vol 16 No 3 (2015)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

Rhiphicephalus (Boophilus) microplus is important obligatory blood feeding ectoparasites transmittingmany different viral, bacterial and protozoan and plays a role as a vector of Babesia sp., The leria sp. andAnaplasma sp. in cattle. The accuracy in identifying and distinguishing interspecies and intraspeciesdiversity among parasites is needed to understand the epidemiology, biology and capacity as a vector.Variations in the DNA base sequence of the internal transcribed spacer region2 (ITS 2) has been used asa molecular marker for identification in an effort to determine phylogenetic relationships. The aim of thisstudy was to determine the ITS 2 gene nucleotide sequence of R. microplus, which was expected to beuseful for accurate identification the parasite diversity and phylogenetic relationship among many differentspecies. DNA amplification was conducted using BOO2 forward dan BOO2 reverse primers. The DNAsamples containing ITS2 region fragment of 1099 nt were derived from the nucleotide sequence multiplealignments of R.microplus and other ticks genes obtained from Gene bank using Clustal W software, andthen analyzed using the MEGA program version 6. Genetic distances based on nucleotide sequence weredetermined with Kimura 2-parameter method producing the smallest genetic distance of 0 % and 1.2 %.Construction of phylogenetic trees using the Neighbor joining method showed that ticks from variousregions in Indonesia was species complex which have a closer with R.microplus isolates from India, Laos,South Africa, China and Australia R.australis origin.
Keragaman Genetik Gen Penyandi Dehydrogenase Sub-unit 3 Mitokondria pada Monyet Hantu (Tarsius sp.) (GENETIC DIVERSITY OF MITOCHONDRIAL DEHYDROGENASE SUB-UNIT 3 GENE ON TARSIUS SP.) Rini Widayanti; Niken Satuti Nur Handayani; I Made Budiarsa
Jurnal Veteriner Vol 12 No 1 (2011)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

Tarsius is an endemic species in Indonesia but is is getting threatened. In-situ and ex-situ conservationof this species would yield better results when its genetic information and diversity determined. Theobjective of this ressearch was to study the genetic diversity of ND3 gene of Tarsius sp. Based on sequencingof PCR products using primer ND3F and ND3R resulted in 437 nts base sequence. Results of ND3 fragmentssequencing were put on multiple alignment with other primates from Genbank using of software ClustalW, and then were analyzed using MEGA program version 4.1. A different nucleotide site was found(nucleotide no. 24). The genetic distance based on nucleotide ND3 was calculated using Kimura 2-parametermodel. The genetic distance showed the smallest genetic distance 0%, the biggest 0,03% and average0,01%. The phylogenetic tree using neighbor joining method based on the sequence of nucleotide ND3 genecould not be used to differentiate among T. Dianae (from Central Sulawesi), T. Spectrum (from NorthSulawesi) and T. bancanus (from Lampung, South Sumatera).
Identification Species of Myxobolus from Gill of Cyprinus carpio in East Java (IDENTIFIKASI MYXOBOLUS SP YANG DIPEROLEH DARI INSANG IKAN KARPER DI JAWA TIMUR) Agus Priyono; Kurniasih .; Rini Widayanti; Ayuda Dyah Nurekawati
Jurnal Veteriner Vol 14 No 1 (2013)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

The aim of study was to identify  Myxobolus sp. obtained from the gills of carp (Cyprinus carpio) ofEast Java, Indonesia. The cysts containing spores were collected from the gills of carp fish. The spores wereexamined by wet mounts preparation, fixed with ethanol absolute solution for molecular analysis. Thespores had a transparent membrane, the shell, composed of two valves. The sutural ridge running betweenthe valves. It was two anterior polar capsules, each consisted of a coiled polar filament. An iodinophilicvacuole and sporoplasm nuclei was located in posterior part. DNA Sequenses 18S rDNA followed byphylogenetic tree demonstrated that Myxobolus sp from Blitar was different from Myxosoma cerebralis ofthe Gene Bank. Myxosoma cerebralis was not found  in the fresh water fish in Indonesia.
Kajian Molekular Tarsius sp. Pada Gen Penyandi Cytochrome Oxidase Sub-unit 2 Mitokondria Rini Widayanti; Niken Satuti Handayani; I. Made Budiarsa
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 15, No 1 (2010): February 2010
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24002/biota.v15i1.2653

Abstract

Tarsius is an endemic species in Indonesia that is endangered. In-situ and ex-situ conservation of this species would yield better results if this genetic make up and diversity is determined. The objective of this ressearch was to study the specific genetic marker on COX2 gene of Tarsius sp. Sequencing of PCR product using primer COX2F and COX2R resulted in base sequence of 513 nts. Results of COX2 fragments sequencing were put on multiple alignment with other primates from Genbank with aid of software Clustal W, and were analyzed using MEGA program version 4.1. Eight different amino acid sites were found (amino acid no. 5, 6, 13, 14, 30, 35, 44 and 168). The genetic distance based on nucleotide COX2 calculated using Kimura 2-parameter model indicated that in the smallest genetic distance 0%, biggest 6.8% and average 2.3%. The phylogenetic tree using neighbor joining method based on the sequence of nucleotide and amino acid COX2 reveded differentiation among Tarsius from Lampung and Tarsius from Sulawesi, but could not be used to differentiate among T. dianae (from Central Sulawesi) and T. spectrum (from North Sulawesi).
Kajian Penanda Genetik Tarsius bancanus dan Tarsius spectrum dengan Sekuen D-Loop Parsial DNA Mitokondria Rini Widayanti; Dedi Duryadi Solihin
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 12, No 3 (2007): October 2007
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24002/biota.v12i3.2804

Abstract

The objective of this research was to study the specific genetic marker on D-loop region of Tarsius bancanus and Tarsius spectrum. The sequencing of PCR product using primer DLTARPROF on D-loop resulted in base sequence of 270 nts. Result of D-loop fragments sequencing was put on multiple alignment with other primates from Genbank with the aid of software Genetyc-Win Version 3.0 and Clustal W, and was analyzed using MEGA program version 3.1. The genetic distance was based on nucleotide D-loop, the smallest genetic distance was 0% and the biggest was 11.8% and the average was 2.3%. The phylogenetic tree using neighbor Joining Method based on some nucleotide sequence on D-loop region could not be used to differentiate between Tarsius bancanus and Tarsius spectrum.
Co-Authors Agus Priyono Amalia Rezeki Ana Sahara Aris Haryanto Aris Purwantoro Ayuda Dyah Nurekawati Charles Rangga Tabbu Dedi Duryadi Solihin Dondin Sajuthi DYAH PERWITASARI -FARAJALLAH Heri Budi Santoso Irma Padeta Joko Prastowo Kurniasih . Laksono Trisnantoro Niken Satuti Handayani Niken Satuti Nur Handayani TARUNI SRI PRAWAST MIEN KAOMINI ANY ARYANI DEDY DURYADI SOLIHIN Teguh Budipitojo Wayan Tunas Artama Widya Asmara Wisnu Nurcahyo Yuriadi - Yuriadi .