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All Journal Health Notions THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST Manuju : Malahayati Nursing Journal Journals of Ners Community JURNAL PENELITIAN KESEHATAN Medicra (Journal of Medical Laboratory Science/Technology) JOURNAL OF COMMUNITY MEDICINE AND PUBLIC HEALTH RESEARCH Jurnal Rakat Sehat : Pengabdian Kepada Masyarakat

Pestariati Pestariati

Jurusan Teknologi Laboratorium Medis, Poltekkes Kemenkes Surabaya

Author-ID : 2536569

Dentistry Health Professions Medicine & Pharmacology Nursing Public Health Veterinary

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Articles

Solid Media (Lowenstein Jensen) and Liquid Media (Mycobacteria Growth Indicator Tube) Usage Against Mycobacterium tuberculosis Culture in Sputum Suspect Tuberculosis Pestariati Pestariati; Meilinda Rachmawati
Health Notions Vol 5, No 6 (2021): June
Publisher : Humanistic Network for Science and Technology (HNST)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33846/hn50607

Tuberculosis examination using Lowenstein Jensen (LJ) media, requires an incubation time of 8 weeks after inoculation. The objective of this studi to compare result media Mycobacterium tuberculosis proliferation on solid media (LJ) and liquid media (MGIT) on sputum of suspected TB. The liquid culture method was Mycobacteria Growth Indicator Tube (MGIT). This method was fully automatic and non-radiometric. MGIT contains 7.0 ml of modified Middlebrook 7H9 broth. Culture results will come out in 42 days if there was no bacterial growth. Comparative research on 30 sputum suspects of TB were examined at the Surabaya Health Laboratory Center on 03 January to 20 March 2020. The sputum samples were examined through the stages of decontamination, homogenization, concentration and inoculated on LJ media and MGIT media and incubated at 370C. Identification of the colonies that grew with ZN staining and MPT 64 immunochromatography test. The results of the Wilcoxon Signed Rank Test statistical test obtained p-value of 0.157 (p> 0.05). It can be concluded that there is no difference in the culture results of Mycobacterium tuberculosis on solid media (Lowenstein Jensen) and liquid media (Mycobacteria Growth Indicator Tube). Keywords: Mycobacterium tuberculosis; Lowenstein Jensen (LJ);Mycobacteria Growth Indicator Tube (MGIT)

Sorgum Agar (Sorghum bicolor) as Substitute Nutrient Agar Media for Cultivation Escherichia coli Pestariati Pestariati; Mufidah Mufidah
Health Notions Vol 5, No 07 (2021): July
Publisher : Humanistic Network for Science and Technology (HNST)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33846/hn50706

Media (nutrient agar) is a medium that is often used for bacteriological examinations and the price is relatively more expensive, Sorghum bicolor materials that are easy to get and cheap. Sorghum contains carbohydrates and protein which are suitable for manufacturing agar nutrients. This research was an experimental quantitative analysis laboratory. The research used Sorghum bicolor flour as carbohydrates and proteins in nutrient agar media. This study used variations in the mass of sorghum flour 2.50 grams, 3.75 grams, 5.00 grams, and 6.25 grams. The average number of bacterial colonies at mass variations was 2.50 grams, 3.75 grams, 5.00 grams, and 6.25 grams, namely 70.75 × 1013 CFU / mL, 60.75 × 1013 CFU / mL, 49, 25 × 1013 CFU / mL, and 42 × 1013 CFU / mL with significant differences in the value of p = 0.000 or

RESISTANCE PATTERN OF Escherichia Coli AGAINTS ANTIBIOTICS IN URINARY TRACT INFECTION PATIENTS IN RSUD DR. SOETOMO SURABAYA Novi Ariana; Pestariati Pestariati; Retno Sasongkowati; Debby Kusumaningrum
Journal of Community Medicine and Public Health Research Vol. 1 No. 2 (2020): Journal of Community Medicine and Public Health Research
Publisher : Universitas Airlangga

Pathogenic bacteria cause Urinary Infection imore or lessi about 85 - 95% are bacteria Escherichia coli. The treatment of Urinary Tract Infection (UTI) requires adequate supportive therapy and antibiotics, but antibiotic resistance is an obstacle in UTI treatment. This study aims to determine the types of antibiotics that are resistant to Escherichia coli in patients with UTI. This is an observational study with a descriptive method to determine the type of antibiotic-resistant to Escherichia coli in patients with UTI in the Laboratory of Clinical Microbiology RSUD Dr. Soetomo. Antibiotics that were resistant to Escherichia coli in UTI patients were cefazolin (80.49%), Amoxicillin Clavulanic Acid (73.17%), Ampicillin (73.17%), Tetracycline (68.29%), while antibiotics that were resistant to Escherichia coli ESBL was Amoxicillin Clavulanic Acid (100%), Ampicillin (100%), Cefazolin (99.7%), Aztreonam (99.7%), Ceftazidime (99.7%). The prevalence of Escherichia coli in UTI patients was 27.5%, while Escherichia coli ESBL was 72.5%. Based on the results of the research that has been obtained, it is better to do research on antibiotic resistance patterns periodically.

PENGARUH PENYIMPANAN URINE TERHADAP JUMLAH lEUKOSIT DAN ERITROSIT PADA PENDERITA INFEKSI SALURAN KEMIH DENGAN METODE SY (STANDARD YIELD) Edy Haryanto; Pestariati .; Anik Handayati; Sri Sulami Endah Astuti
JURNAL PENELITIAN KESEHATAN Vol 13, No 1 (2015): Jurnal Penelitian Kesehatan
Publisher : Poltekkes Kemenkes Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1482.879 KB)

Urinary tract infection ( un ) is a pathological condition that is often encountered and marked with urinesediment results indicate nitrite, leucocytes and positive bacteria. In some hospitals, inpatient specimendelivery is often delayed, causing delayed examination. Urine samples should be examined, therefore itis kept at room temperature, lysis of erythrocytes and leukocytes and urine will turn into alkaline. Thisstudy aims to prove how much the influence of the long treatment of urinary tract infection to the numberof leukocytes and erythrocytes urine sediment . "This type of research is quantitative analysis " todetermine the number of leukocytes and erythrocytes urine sediment urinary tract infection sufferers whoimmediately performed ( 0 hours) , stored for 2 hours, 3 hours and 4 hours using SY ( standard yield) .The results of the examination number of leukocytes to the 20 sediment samples with immediate unpatients obtained an average of 59 cells / mL , after the delay of 2 hours earned the average of 35 cells /mL, 3 hours on the average of 18 cells / mL and 4 hours obtained an average of 12 cells / uL. The resultsof the examination of erythrocyte sediment amount to ISK 20 patient samples obtained immediatelyperformed the average of 20 cells / mL , after the delay of 2 hours earned the average of 14 cells / mL , 3hours on the average of 9 cells / mL and 4 hours obtained the average of 6 cells / mL . From the Post Hoctest results obtained three treatment groups proved no longer influence to urinary tract infections.suspension 0 hour, 2 hours, and 3 hours to the number of leukocytes and erythrocytes urine sediment.There is no longer influence to urinary tract infections suspension 3 hours and 4 hours on the number ofleukocytes and erythrocytes urine sediment.Key words: ISK, Sedimen urtn, /eukocit dan erithrocit.

Studi Komparasi Hasil Pemeriksaan Mycobacterium Tuberculosis Pada Pasien Suspek Tuberkulosis Dengan Menggunakan Tes Cepat Molekuler dan Mikroskopis di RSUD Karangasem I Nyoman Krisna Wicaksana; Pestariati Pestariati; Syamsul Arifin
Journals of Ners Community Vol 13 No 1 (2022): Journals of Ners Community
Publisher : Fakultas Ilmu Kesehatan Universitas Gresik

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55129/jnerscommunity.v13i1.1746

Tuberkulosis merupakan suatu penyakit kronik menular yang disebabkan oleh Mycobacterium tuberculosis yang menyebabkan jumlah kematian global mencapai 1.3 juta pada tahun 2020 dan ditambah parah setelah adanya pandemi COVID-19 yang mengakibatkan terbatasnya layanan tuberkulosis, kasus di Indonesia selalu mengalami peningkatan tiap tahunnya, tahun 2019 ditemukan sebanyak 543.874 kasus meningkat dari 420.994 kasus di tahun 2017. Metode pemeriksaan yang banyak digunakan di negara endemik tuberkulosis adalah Mikroskopis, namun terdapat beberapa kekurangan dalam penggunaanya dibandingkan dengan metode Tes Cepat Molekuler. Penelitian ini bersifat observasional dengan pendekatan cross sectional yang bertujuan untuk mengetahui perbedaan hasil pemeriksaan Mycobacterium tuberculosis pada pasien suspek tuberkulosis dengan menggunakan Tes Cepat Molekuler dan Mikroskopis di RSUD Karangasem. Penelitian ini dilaksanakan dari bulan Desember 2021 sampai April 2022 menggunakan sampel sputum yang diperoleh dari RSUD Karangasem. Penelitian menunjukan adanya perbedaan hasil sensitivitas dan spesifisitas pada dua metode. Sensitivitas metode Tes Cepat Molekuler lebih tinggi daripada metode Mikroskopis (100% dan 75%) sedangkan nilai spesifisitas metode Mikroskopis lebih tinggi daripada metode Tes Cepat Molekuler (100% dan 95%).

Potensi Ikan Tongkol Dan Ikan Lele Sebagai Media Alternatif Pertumbuhan Escherichia coli Muhammad Sholakhuddin Al-Ayubi; Pestariati Pestariati; Anita Dwi Anggraini; Diah Titik Mutiarawati
THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST Vol 5, No 2 (2022): The Journal Of Muhammadiyah Medical Laboratory Technologist
Publisher : Universitas Muhammadiyah Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30651/jmlt.v5i2.14407

Media pertumbuhan yang mengandung nutrisi yang dibutuhkan oleh bakteri dan yang sering digunakan untuk pertumbuhan bakteri yaitu media Nutrient Agar. Mahalnya harga media pertumbuhan bakteri mendorong para peneliti untuk menggunakan media pertumbuhan bakteri yang terbuat dari bahan-bahan alami yang mudah didapatkan dan tidak mengeluarkan biaya yang mahal. Ikan tongkol memiliki kandungan protein sebesar 26,46% dan ikan lele yang mempunyai kandungan protein sebesar 24,63%. Escherichia coli merupakan bakteri Gram negatif bersel satu, bersifat patogen dan dapat menyebabkan penyakit diare. Jenis penelitian yang dilakukan adalah Eksperimen. Hasil penelitian ini menunjukkan jumlah rata-rata koloni terbanyak pada pertumbuhan Escherichia coli dengan menggunakan media alternatif Ikan Tongkol yaitu pada variasi massa 5 gram yaitu 118 x 10-13 CFU/mL, sedangkan jumlah rata-rata koloni terbanyak pada pertumbuhan Escherichia coli dengan menggunakan media alternatif Ikan Lele yaitu pada variasi massa 5 gram yaitu 104 x 10-13 CFU/mL. Media substitusi Nutrient Agar dari tepung daging Ikan Tongkol dan Ikan Lele dengan variasi massa 1 gram, 3 gram dan 5 gram dapat menumbuhkan koloni bakteri Escherichia coli dengan jumlah dan karakteristik yang berbeda. Hal tersebut disebabkan oleh kandungan protein di dalam tepung daging Ikan Tongkol dan Ikan Lele yang dapat memenuhi kebutuhan nutrisi pertumbuhan Escherichia coli. Ikan tongkol dan ikan lele mempunyai potensi sebagai bahan dasar pembuatan media alternatif untuk pertumbuhan bakteri Escherichia coli ditandai dengan adanya pertumbuhan koloni bakteri pada media alternatif ikan tongkol dan ikan lele. Kata Kunci : Ikan Tongkol, Ikan Lele, Nutrient Agar, Escherichia coli

Deteksi Gen Tem (Temoneira) dari Isolat Klinis Escherichia Coli Penghasil Extended Spectrum Beta Lactamases (ESBL) Pasien Penderita Infeksi Saluran Kemih Ida Lestari; Pestariati Pestariati; Sri Sulami Endah Astuti
Malahayati Nursing Journal Vol 5, No 1 (2023): Volume 5 Nomor 1 2023
Publisher : Universitas Malahayati Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33024/mnj.v5i1.7832

ABSTRACT Urinary tract infection (UTI) is a most common infectious disease. The prevalence of UTIs in Indonesia is estimated at 180.000 per year and can affect people of all ages. In East Java, the number of cases of Urinary Tract Infection reaches 3-4 cases per 100,000 population per year. The most dominant urinary tract infection is caused by Escherichia coli bacteria from the multidrug-resistant Gram-negative group, such as producing of Extended Spectrum Beta Lactamases (ESBL). Extended Spectrum Beta-Lactamases (ESBLs) are Beta- Lactamase enzymes which ability can cause bacteria resistant to penicillin, cephalosporin generation 1, 2 and 3, and aztreonam (but not cefamycin and carbapenem). The causes of bacteria producing ESBL enzymes is the presence of ESBL gene. The one of ESBL gene groups which responsibility to producing ESBLs to hydrolyzing beta-lactamase antibiotics is temoneira enzyme (TEM). This study aim to determine the presence of TEM gene in Escherichia coli clinical isolates producing Extended Spectrum Beta-Lactamases (ESBL) from UTI patients urine in RSPAL Dr. Ramelan Surabaya. This type of research is descriptive observational with cross sectional approach. The sample used in this study was the clinical isolate Escherichia coli ESBL, as many as thirty isolates from UTIs patient urine in RSPAL Dr. Ramelan Surabaya. The detection of TEM gene used the PCR (Polymerase Chain Reaction) method. The result of the detection of TEM gene found were 3% (1/30) in RSPAL. Dr Ramelan Surabaya. The analysis of distribution of Escherichia coli ESBL bacteria in patient care room was obtained 54% (16/30) in ICU rooms, 43% (1/30) Non ICU, 3% (1/30) PICU rooms, and not found 0% in the NICU rooms, and outpatient clinic. Keywords: Urinary Tract Infection (UTI), Antimicrobial Resistance, Escherichia Coli Clinical Isolate, ESBL, and TEM (Temoneira) Gen ABSTRAK Infeksi saluran kemih (ISK) merupakan penyakit infeksi yang sangat umum terjadi. Angka kejadian kasus ISK di Indonesia diperkirakan sebesar 180.000 kasus pertahun dan dapat menjangkit semua orang dari segala usia. Sedangkan untuk wilayah Jawa Timur jumlah kasus Infeksi Saluran Kemih mencapai 3-4 kasus per 100.000 penduduk per tahun. Bakteri Escherichia coli dari golongan gram negatif multidrugresiten, seperti penghasil Extended Spectrum Beta Lactamases (ESBL) yang paling dominan menyebabkan infeksi saluran kemih. Extended Spectrum Beta- Lactamases (ESBL) adalah enzim Beta- Lactamase yang kemampuanya dapat menyebabkan bakteri resisten terhadap penisilin, sefalosporin generasi 1, 2, dan 3, serta aztreonam (tetapi tidak terhadap sefamisin dan karbapenem). Penyebab bakteri menghasilkan enzim ESBL karena adanya gen yang mengkode ESBL. Kelompok gen ESBL yang bertanggungjawab menghasilkan ESBL dalam menghidrolisis antibiotik beta-lactamase salah satunya adalah enzim temoneira (TEM). Tujuan penelitian ini yaitu untuk mengetahui keberadaan gen TEM pada isolat klinis Escherichia coli penghasil Extended Spectrum Beta-Lactamases (ESBL) dari urin pasien ISK di RSPAL Dr. Ramelan Surabaya. Jenis penelitian ini yaitu obervasional deskriptif dengan pendekatan cross sectional. Total sampel penelitian ini yaitu sebanyak tiga puluh isolat klinis Escherichia coli ESBL. Deteksi gen TEM menggunakan metode PCR (Polymerase Chain Reaction). Hasil deteksi gen TEM yang berhasil ditemukan sebesar 3% (1/30) di RSPAL. Dr. Ramelan Surabaya. Analisis penyebaran bakteri Escherichia coli ESBL di ruang perawatan didapatkan 54% (16/30) di ruang ICU, 43% (13/30) Non ICU, 3% (1/30) PICU serta tidak ditemukan 0% di ruang NICU, dan klinik rawat jalan. Kesimpulan pada penelitian ini adalah itemukan Gen TEM pada isolat Klinis E.coli dari urine pasien ISK sebnyak satu isolat (3%) di ruang perawatan Non ICU. Kata Kunci: Infeksi Saluran Kemih (ISK), Resistensi Antimikroba, Isolat Klinis Escherichia coli, ESBL, dan gen TEM (temoneira)

Ability Analysis Of Waste Milkfish (Chanos chanos) As Alternative Medium Of Nutrient Agar On Escherichia coli And Staphylococcus aureus Growth Gusti Naila Rachmawati Gufron; Pestariati; Syamsul Arifin
Medicra (Journal of Medical Laboratory Science/Technology) Vol 5 No 2 (2022): December
Publisher : Universitas Muhammadiyah Sidoarjo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21070/medicra.v5i2.1646

Waste milkfish got a lot from the residual processed milkfish production of Sidoarjo. The intestines, liver, and kidneys of waste milkfish contain protein, carbohydrates, and fat. The abundant availabilities were usable as a bacterial growth medium, including Nutrient Agar. Nutrient Agar has a high price and is produced by many foreign companies that can trigger a hard availability in laboratories of domestic healthcare, especially in frontier, outermost, and underdeveloped areas. This research aims to analyze the ability of waste milkfish as an alternative medium for Nutrient Agar on the growth of Escherichia coli and Staphylococcus aureus. The ability has been observed by the characteristics and number of bacterial colonies. This research was held in the bacteriology laboratory of the Medical Laboratory Technology Department, Health Polytechnic of Surabaya health ministry. The research type was True-experimental with a post-test-only control group design. Based on the preliminary test results, the waste milkfish mass variations were found in 7 grams, 7.5 grams, 8 grams, 8.5 grams, and 9 grams. Bacteria have been inoculated by the spread plate method, bacterial colony growth has been calculated by the Total Plate Count method. The data were analyzed by the Kruskal-Wallis test with a Sig. value <0.05 that there were differences in the number of Escherichia coli and Staphylococcus aureus colonies in milkfish waste media with various mass. The results showed that the best ability of waste milkfish as an alternative medium for Nutrient Agar on bacterial growth was found in 9 grams mass variation. the average number of Escherichia coli colonies were 186 × 1013 CFU/mL and Staphylococcus aureus colonies were 188 × 1013 CFU/mL.

Pemanfaatan Teri Jengki (Stolephorus indicus) Dan Layang Deles (Decapterus macrosoma) Sebagai Media Alternatif Pertumbuhan Escherichia coli Armelia Gitasari Nurhasanah; Pestariati Pestariati; Anita Dwi Anggraini; Sri Sulami Endah Astuti
THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST Vol 6, No 1 (2023): The Journal Of Muhammadiyah Medical Laboratory Technologist
Publisher : Universitas Muhammadiyah Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30651/jmlt.v6i1.14452

ABSTRAK Nutrient agar merupakan jenis media umum yang sering digunakan pada laboratorium, harga media ini relatif mahal. Nutrient agar berbentuk padat, terdiri atas campuran ekstrak daging, pepton serta agar. Ikan teri jengki dan ikan layang deles jumlahnya melimpah di Indonesia. Ikan teri jengki (Stolephorus indicus) mengandung protein 51.54%, sementara ikan layang deles (Decapterus Macrosoma) mengandung protein sebesar 57,89%. Escherichia coli digunakan sebab bakteri ini merupakan salah satu jenis mikroorganisme kontrol positif yang direkomendasikan berdasarkan Data Sheet Nutrient Agar OXOID. Penelitian ini bersifat eksperimental dengan tiga perlakuan pada masing-masing bahan yaitu variasi massa 3 gram, 4 gram dan 5 gram yang dilakukan di Laboratorium Bakteriologi Jurusan Teknologi Laboratorium Medis Poltekkes Kemenkes Surabaya. Hasil penelitian menunjukkan jumlah koloni rata-rata Escherichia coli yang tumbuh pada kontrol positif sebanyak 118 x 1013, sementara pada media ikan teri jengki variasi massa 3 gram adalah 72 x 1013 CFU/mL, 4 gram sebanyak 85 x 1013 CFU/mL, 5 gram sebanyak 94 x 1013 CFU/mL. Pada media ikan layang deles variasi 3 gram sebanyak 88 x 1013 CFU/mL, 4 gram sebanyak 96 x 1013 CFU/mL, 5 gram 108 x 1013 CFU/mL. Variasi massa yang paling baik dan mendekati hasil kontrol positif (nutriet agar) pada ikan teri jengki dan ikan layang deles adalah variasi massa 5 gram.Kata kunci: ikan teri jengki, ikan layang deles, Escherichia coli, nutrient agar

Kolesterol Dan Tekanan Darah Lansia Di Posyandu Abiyoso Polkesbaya Juliana Christyaningsih; Luthfi Rusyadi; Minarti; Mamik; Pestariati; Diah Titik Mutiarawati; Suliati; Retno Sasongkowati; Evy Diah Woelansari; Suhariyadi; Wisnu Istanto; Lully Hanni Endarini; Museyaroh
JURNAL RAKAT SEHAT (JRS) : Pengabdian Kepada Masyarakat Vol 2 No 2 (2023): Jurnal Rakat Sehat: Pengabdian Kepada Masyarakat
Publisher : UPPM Poltekkes Kemenkes Banjarmasin

Show Abstract | Download Original | Original Source | Check in Google Scholar

As people age, the physical state of the human body will gradually deteriorate due to a decrease in the quantity of cells present, leading to a gradual reduction in functionality referred to as the aging process. In elderly humans (aged more than 60 years) excessive cholesterol levels and high blood pressure are one of the causes of diseases such as heart disease which has been recognized by WHO as the number one cause of death in the world. The buildup of fatty deposits on the linings of blood vessels has the potential to lead to obstructions within the blood vessels. The objective of this initiative is to enhance understanding regarding the hazards associated with cholesterol. This outreach effort took place in July 2023, conducted at Posyandu Abiyoso, Surabaya Health Polytechnic. The activity consists of several processes 1). conducting a survey of the location of the Abiyoso posyandu of the Surabaya Health Polytechnic, 2). Opening registration and taking participants' personal data, 3) conducting participant health interviews, 4). Checking cholesterol levels and blood pressure of participants. From the community service activities carried out, it was found that 42 of the 65 (64.6%) participants who took part in the examination at the integrated service post had high cholesterol levels, and 60 of the 65 (92.3%) participants had high blood pressure or hypertension. By conducting health checks on cholesterol levels and blood pressure, there is an expectation that the society will come to realize and comprehend. the dangers of hypertension and hypercholesterolemia so that the community can prevent the occurrence of sustainable diseases.

Co-Authors Anik Handayati Anita Dwi Anggraini Anita Dwi Anggraini Armelia Gitasari Nurhasanah Debby Kusumaningrum Diah Titik Mutiarawati Diah Titik Mutiarawati Edy Haryanto Gusti Naila Rachmawati Gufron I Gusti Bagus Wiksuana I Nyoman Krisna Wicaksana Ida Lestari Juliana Christyaningsih Lully Hanni Endarini Luthfi Rusyadi Mamik Meilinda Rachmawati MINARTI Mufidah Mufidah Muhammad Sholakhuddin Al-Ayubi Museyaroh Novi Ariana Retno Sasongkowati Retno Sasongkowati Sri Sulami Endah Astuti Sri Sulami Endah Astuti Sri Sulami Endah Astuti Suhariyadi Suliati Syamsul Arifin Wisnu Istanto Woelansari, Evy Diah

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