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All Journal Ensiklopedia of Journal INDONESIAN JOURNAL OF MEDICAL LABORATORY SCIENCE AND TECHNOLOGY
Chairani
Department Of Medical Laboratory Technology, Fakultas Of Health Science, Universitas Perintis Indonesia, Padang, Indonesia
Arts Humanities Computer Science & IT Health Professions Law, Crime, Criminology & Criminal Justice Social Sciences

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COMBINATION TEST OF CHINESE LEAF EXTRACT (Leucaena leucocephala folium) AND ALOE VERA INHIBITING GROWTH Escherichia coli Putra Rahmadea Utami; Chairani; Hendra Yudha
JURNAL INDONESIA DARI ILMU LABORATORIUM MEDIS DAN TEKNOLOGI Vol 2 No 2 (2020): Increasing Research in Diagnostic Laboratory Testing
Publisher : Universitas Nahdlatul Ulama Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33086/ijmlst.v2i2.1577

Abstract

Chinese petai (Leucaena leucocephala folium) and aloe vera (Aloe vera L.) have medicinal properties among the plants.  The objective of this study was to determine the number of inhibitory zones produced by the ethanol extract of Chinese petai and aloe vera on the growth of Escherichia coli. The research method was In-vitro Experimental Laboratory research design with the Kirby Bauer method. The samples used was Chinese petai and aloe vera with pure strains of E. coli. One Way ANOVA was used to compare the differences in inhibition of Chinese petai and aloe vera on the growth of E. coli. The results of a combination of Chinese petai and aloe vera extract test showed that there were significant differences in the concentration of 25 g, 50 g, 75 g, and 100 g (p <0.05). The ethanol extract of Chinese petai and aloe vera can inhibit the growth of E. coli. From the results of this study found that there was an interaction on the combination of ethanol extract of Chinese petai and aloe vera inhibiting the growth of E. coli with the most effective concentration being 100 g/mL. This study can find out the benefits of petai cina and aloe vera also the public will know the benefits and efficacy of Chinese petai and aloe vera leaves in medicine.
STRUKTUR HISTOLOGI DAN JUMLAH SEL GOBLET PADA SEDIAAN HISTOPATOLOGIS RADANG USUS BESAR DENGAN PEWARNAAN HEMATOXYLIN-EOSIN (HE) DAN PERIODIC ACID-SCHIFF (PAS) Chairani Chairani; Fitra Wahyuni; Tofrizal Tofrizal; Salsabila Salsabila
Ensiklopedia of Journal Vol 4, No 3 (2022): Vol 4 No. 3 Edisi 2 April 2022
Publisher : Lembaga Penelitian dan Penerbitan Hasil Penelitian Ensiklopedia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (316.544 KB) | DOI: 10.33559/eoj.v4i3.137

Abstract

Abstract: Goblet cells and the mucus they produce serve as an important barrier to prevent pathogens invading the mucosal tissue that can cause inflammation. In the inflamed tissue, will have changed in the structure and number of goblet cells. Hematoxylin-Eosin (HE) staining is a standard stain used to examine the histological structure of tissues while Periodic Acid-Schiff (PAS) is a histological stain other than HE that can stain carbohydrates and carbohydrate-rich macromolecules. The purpose of this study was to observe differences in the structure and number of goblet cells in the inflammatory colon using HE and PAS staining. This study used 27 samples of paraffin block of rat colon tissue which were divided into three groups, namely the normal group, acute intestinal inflammation, and chronic intestinal inflammation. The tissue samples were then stained with HE and PAS staining techniques. In this study, statistical analysis of an independent t-test and Mann-Whitney test was used to observe differences in the number of goblet cells in inflammatory colon tissue using HE and PAS staining in each group. This study concludes that the number of goblet cells that can be observed in inflammatory colon tissue using HE and PAS staining is significantly different with a p-value <0.05 where the average number of goblet cells using PAS staining is higher than using HE.Keywords: goblet cells, inflammatory colon, Hematoxylin-Eosin, Periodic Acid-Schiff.
Co-Authors Fitra Wahyuni Hendra Yudha Putra Rahmadea Utami Salsabila Salsabila Tofrizal Tofrizal