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All Journal Jurnal Kesehatan Andalas Ensiklopedia of Journal Health and Medical Journal Science Midwifery Jurnal Ilmu Kesehatan Indonesia
Tofrizal Tofrizal
Bagian Patologi Anatomi, Fakultas Kedokteran Universitas Andalas, RSUP Dr. M. Djamil, Padang, Indonesia
Arts Humanities Biochemistry, Genetics & Molecular Biology Computer Science & IT Dentistry Health Professions Immunology & microbiology Law, Crime, Criminology & Criminal Justice Medicine & Pharmacology Nursing Public Health Social Sciences Veterinary

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Ekspresi Protein Sitoplasmik SOCs2 pada Adenokarsinoma Kolorektal: Studi Imunohistokimia Nita Afriani Azmal; Shinta Ayu Intan; Tofrizal Tofrizal
Jurnal Kesehatan Andalas Vol 11, No 1 (2022): Online March 2022
Publisher : Fakultas Kedokteran, Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jka.v11i1.1982

Abstract

 Inflammatory cytokines have a role in triggering cell signals in colorectal cancer. They increase signaling pathways into cells via the Janus kinase pathway (JAK) - signal transducer and activator of transcription (STAT) -suppressor of cytokine signaling (SOCs) JAK-STAT. SOCs2 protein is a cytoplasmic protein that can inhibit activation of JAK-STAT pathway so that it prevents cascade of signals into the nucleus. This protein is thought to have decreased in adenocarcinoma colorectal. Objectives: To reveal the expression of SOCs2 protein in 37 paraffin block samples in Anatomy Pathology Department Medical Faculty Andalas University. Methods: Data were taken from medical records and histopathology was assessed by HE. SOCs2 expression was evaluated by immunohistochemistry using SOCs2 polyclonal antibody (bs-1896R). Cytoplasmic expression was counted using Image J software. Results: The average of age was 56.57 years, 21 samples (55.8%) were male, depth of invasion was mostly in the tunica muscularis (T3), and 7 cases of malignancy (18.9%) were poorly differentiated. SOCs2 expression was  5 cases (13.5%).   Conclusion: There was SOCs2 expression in a small proportion of colorectal adenocarcinoma.Keywords:  SOCs2 protein, cytoplasmic inhibitor, JAK-STAT, cell signaling
STRUKTUR HISTOLOGI DAN JUMLAH SEL GOBLET PADA SEDIAAN HISTOPATOLOGIS RADANG USUS BESAR DENGAN PEWARNAAN HEMATOXYLIN-EOSIN (HE) DAN PERIODIC ACID-SCHIFF (PAS) Chairani Chairani; Fitra Wahyuni; Tofrizal Tofrizal; Salsabila Salsabila
Ensiklopedia of Journal Vol 4, No 3 (2022): Vol 4 No. 3 Edisi 2 April 2022
Publisher : Lembaga Penelitian dan Penerbitan Hasil Penelitian Ensiklopedia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (316.544 KB) | DOI: 10.33559/eoj.v4i3.137

Abstract

Abstract: Goblet cells and the mucus they produce serve as an important barrier to prevent pathogens invading the mucosal tissue that can cause inflammation. In the inflamed tissue, will have changed in the structure and number of goblet cells. Hematoxylin-Eosin (HE) staining is a standard stain used to examine the histological structure of tissues while Periodic Acid-Schiff (PAS) is a histological stain other than HE that can stain carbohydrates and carbohydrate-rich macromolecules. The purpose of this study was to observe differences in the structure and number of goblet cells in the inflammatory colon using HE and PAS staining. This study used 27 samples of paraffin block of rat colon tissue which were divided into three groups, namely the normal group, acute intestinal inflammation, and chronic intestinal inflammation. The tissue samples were then stained with HE and PAS staining techniques. In this study, statistical analysis of an independent t-test and Mann-Whitney test was used to observe differences in the number of goblet cells in inflammatory colon tissue using HE and PAS staining in each group. This study concludes that the number of goblet cells that can be observed in inflammatory colon tissue using HE and PAS staining is significantly different with a p-value <0.05 where the average number of goblet cells using PAS staining is higher than using HE.Keywords: goblet cells, inflammatory colon, Hematoxylin-Eosin, Periodic Acid-Schiff.
Hubungan Jumlah Eosinofil dengan Ukuran Polip Nasal dan Karakteristik Pasien yang Didiagnosis di Sentra Diagnostik PA FK-UNAND Tahun 2017-2019 Salma Yuri Khairunnisa; Tofrizal Tofrizal; Netti Suharti
Jurnal Ilmu Kesehatan Indonesia Vol 2 No 1 (2021): Maret 2021
Publisher : Fakultas Kedokteran, Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1678.277 KB) | DOI: 10.25077/jikesi.v2i1.291

Abstract

Background: Nasal polyps is a soft masses filled with fluid in nasal mucosa. The incidence is not high, but nasal polyps are related with inflammatory process. Factors that can affect nasal polyps are eosinophils, polyp size, age and gender of the patient. Objective: This study aims to discuss the association between eosinophils with polyp size, age and gender of polyp patient. Methods: The type of this study was an analytic using a cross sectional approach. This research took place from February- October 2020 in the Pathology Anatomy Diagnostic Central of Faculty of Medicine, Andalas University with 19 total sample and this study used total sampling technique. Results: The results based on bivariate analysis of Chi-Square test between amount of eosinophils with polyp size, age and gender of polyps patients have p values (p = 0, 036), (p = 0.264), (p = 1, 00). Conclusion: There is a significant relationship between eosinophils and polyp size. There is no significant relationship between eosinophils with age and gender of patients with nasal polyps in the Pathology Anatomy Diagnostic Central of Faculty of Medicine, Andalas University in 2017- 2019.
Profil Pasien Deep Vein Thrombosis di RSUP Dr. M. Djamil Padang Tahun 2018 Muhammad Farhan; Masrul Syafri; Tofrizal Tofrizal
Jurnal Ilmu Kesehatan Indonesia Vol 2 No 2 (2021): Juni 2021
Publisher : Fakultas Kedokteran, Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1259.561 KB) | DOI: 10.25077/jikesi.v2i2.531

Abstract

Latar Belakang. Deep Vein Thrombosis (DVT) merupakan sebuah kondisi oklusi yang disebabkan oleh terbentuknya trombus patologis pada sirkulasi vena dalam tubuh. DVT merupakan sebuah penyakit yang dapat dicegah namun masih memiliki tingkat morbiditas dan mortalitas yang tinggi. Pemeriksaan menggunakan Ultrasonografi (USG) Doppler merupakan metode yang direkomendasikan untuk menegakkan diagnosis DVT. Objektif. Tujuan penelitian ini adalah untuk mengetahui profil dari pasien DVT di RSUP Dr. M. Djamil Padang pada tahun 2018. Metode. Penelitian ini merupakan penelitian deskriptif observasional dengan pendekatan cross sectional melalui pengumpulan data dari hasil pemeriksaan USG Doppler dan rekam medis. Background. Deep Vein Thrombosis (DVT) is an occlusion caused by patological thrombus that formed at deep vein circulation of the body. DVT is a preventable disease but the morbidity and mortality rate of DVT are still high. The recommended method for diagnosing DVT is by examination with Doppler Ultrasonography (USG) for identificating thrombus at deep vein circulation. Objective. The objective from this study is to identifying clinical profile of DVT patient at Dr. M. Djamil General Hospital Padang in 2018. Method. This is a descriptive observational research with cross sectional method by collecting data from Doppler USG examination report and patient medical record. Results. From this research we found 66 DVT patient, most patients were in the age group of 56-65 years old (23 patients), most thrombus located at lower extremities (49 patients), especially at femoral vein (29 patients). Mostly patients shown with swelling at thrombus location (59 patients), and immobilization was the most risk factor found in the patients (58 patients.) Conclusion. From this research we conclude that mostly DVT patients at M. Djamil General Hospital Padang are elderly and mainly women, mostly DVT located at left leg especially in femoral and poplitea vein and shown with swelling at leg, and mostly DVT patients were having immobilization and malignancy as risk factor. Keywords : Deep vein thrombosis, Doppler USG, clinical profile Hasil. Pada penelitian ini didapatkan 66 pasien DVT di RSUP Dr. M. Djamil Padang pada tahun 2018. Kelompok usia tertinggi ditemukan pada rentang usia 56-65 tahun sebanyak 23 pasien. Lokasi trombus paling sering ditemukan pada tungkai sebanyak 49 pasien, terutama pada vena femoralis sebanyak 29 pasien. Manifestasi klinis yang paling sering ditemukan berupa pembengkakan sebanyak 59 pasien, dan faktor risiko yang paling sering ditemukan imobilisasi sebanyak 58 pasien. Kesimpulan. Kebanyakan pasien DVT di RSUP Dr. M. Djamil Padang berusia lanjut serta memiliki DVT pada tungkai terutama pada vena femoralis sebelah kiri dengan manifestasi pembengkakan, dan memiliki faktor risiko imobilisasi. Perlunya penerapan profilaksis DVT pada pasien yang memiliki resiko tinggi untuk menekan angka kejadian DVT. Kata kunci: Deep vein thrombosis, USG Doppler, profil klinis
Effect of Giving Young Papaya (Carica Papaya L.) Fruit Extract on Endometrial Histology of Female Rats (Rattus Norvegicus) Cecen Suci Hakameri; Tofrizal; Elly Usman
Science Midwifery Vol 9 No 1, Oktober (2020): Science Midwifery
Publisher : Institute of Computer Science (IOCS)

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Young papaya contains several phytochemicals such as saponins, alkaloids and flavonoids. The active compounds in young papaya are thought to be able to cause disruption of the hypothalamic-pituitary pathway which causes disruption of GnRH secretion and affects the ovarian cycle which affects the normal endometrial cycle. The aim of this study was to determine the effect of giving young papaya fruit extract (Carica papaya L.) on endometrial histology of female rats (Rattus norvegicus). This study uses the Post Test Only Control Group method. Total samples were 24 rats (Rattus norvegicus), 2-3 months old and 200-300 grams in weight. Samples were taken randomly, divided into 4 groups. Group (K) without being given treatment. Group (P1) was given 200 mg / 200gr BW of young papaya extract. Group (P2) was given young papaya fruit extract 400 mg / 200gr BW. Group (P3) was given young papaya fruit extract 600 mg / 200gr BW. After 20 days of treatment, the endometrial thickness and density of the endometrium were measured. The results showed that the administration of young papaya fruit extract (Carica papaya L.) caused changes in the thickness of the endometrium and inhibited endometrial gland density. The results of statistical analysis using the one way ANOVA test showed the effect of giving papaya extract on endometrial thickness (p = 0.009) and the effect of giving papaya extract on gland density. It can be concluded that there is an effect of giving young papaya (Carica papaya L.) fruit extract on endometrial thickness and endometrial gland density.
Hubungan Ekspresi Calretinin dan Choline Transporter dengan Keberadaan Sel Ganglion dan Serabut Saraf dalam Diagnosis Penyakit Hirschsprung di RSUP Dr. M. Djamil Padang Tahun 2021-2022 Dini Kusumardani; Noza Hilbertina; Tofrizal Tofrizal; Aswiyanti Asri; Budi Pratama Arnofyan; Husna Yetti
Health and Medical Journal Vol 6, No 2 (2024): HEME May 2024
Publisher : Universitas Baiturrahmah

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33854/heme.v6i2.1484

Abstract

Latar Belakang: Penyakit Hirschsprung merupakan kelainan bawaan yang ditandai dengan tidak adanya sel ganglion di lapisan submukosa atau muskularis propria dari kolon distal dan rektum. Prevalensi kejadiannya 1:5000 kelahiran hidup di populasi umum. Diagnosis pasti penyakit Hirschsprung adalah dengan pemeriksaan histopatologi untuk menentukan adanya sel ganglion dan serabut saraf, tetapi pada sampel jaringan dengan lapisan submukosa yang tidak adekuat dapat sulit untuk menentukan sel ganglion dan serabut saraf. Untuk mengatasi masalah ini dapat dilakukan pemeriksaan imunohistokimia Calretinin dan Choline Transporter. Calretinin, merupakan protein pengikat kalsium yang terlibat dalam pensinyalan kalsium. Calretinin tidak diekspresikan pada segmen aganglionik dan bersifat imunopositif pada sel ganglion atau serabut saraf kolon normal. Choline Transporter merupakan penanda alternatif untuk saraf kolinergik yang efektif dalam mengidentifikasi kasus penyakit Hirschsprung dan secara kualitatif mirip dengan Asetilkolinesterase. Tujuan: penelitian ini bertujuan untuk mengetahui hubungan Calretinin dengan keberadaan sel ganglion dan Choline Transporter dengan keberadaan serabut saraf dalam menegakkan diagnosis penyakit Hirschsprung. Metode: Penelitian ini merupakan penelitian cross sectional dengan sampel sebanyak 70 kasus penyakit Hirschsprung yang telah didiagnosis di Laboratorium Patologi Anatomi RSUP Dr. M. Djamil Padang 2021-2022. Sampel diperoleh secara simple random sampling dari blok paraffin. Dilakukan penilaian ulang pada slaid untuk menilai gambaran histopatologis. Ekspresi Calretinin dan Choline Transporter dilihat dengan pemeriksaan imunohistokimia. Untuk mengetahui korelasi dilakukan analisis statistik bivariat menggunakan uji chi-square dengan tingkat kemaknaan p<0,05. Hasil: Dari 70 kasus, didapatkan ekspresi Calretinin negatif pada 28 kasus (80%) dan memiliki hubungan yang bermakna dengan keberadaan sel ganglion (p = 0,003). Didapatakan ekspresi Choline Transporter positif pada 29 kasus(82,86%) dan memiliki hubungan yang bermakna dengan keberadaan serabut saraf (p = 0,000). Kesimpulan: Penelitian ini menyimpulkan bahwa terdapat hubungan yang bermakna antara ekspresi Calretinin dengan keberadaan sel ganglion dan ekspresi Choline Transporter berhubungan dengan serabut saraf.
Co-Authors Aswiyanti Asri Budi Pratama Arnofyan Cecen Suci Hakameri Chairani Dini Kusumardani Fitra Wahyuni Husna Yetti Intan, Shinta Ayu Masrul Syafri Muhammad Farhan Netti Suharti Nita Afriani Azmal Noza Hilbertina Salma Yuri Khairunnisa Salsabila Salsabila