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All Journal HAYATI Journal of Biosciences Microbiology Indonesia Jurnal Matematika & Sains Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal
Enny Ratnaningsih
Departemen Kimia Fakultas Matematika dan Ilmu Pengetahuan Alam, Institut Teknologi Bandung
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Earth & Planetary Sciences Environmental Science Immunology & microbiology Mathematics Medicine & Pharmacology

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Journal : HAYATI Journal of Biosciences

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Characterization of Extracellular Penicilin G Acylase Produced by A New Local Strain of Bacillus subtilis BAC4 SUPARTONO SUPARTONO; ENNY RATNANINGSIH; SADIJAH ACHMAD; OEI BAN LIANG
HAYATI Journal of Biosciences Vol. 15 No. 2 (2008): June 2008
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (130.423 KB) | DOI: 10.4308/hjb.15.2.71

Abstract

Penicillin G acylase (PGA) which catalyses penicillin G hydrolysis reaction is a key enzyme for the industrial production of penicilin G derivatives used in therapeutics. A new local strain of Bacillus subtilis BAC4 was found capable of producing extracellular PGA. However, characteristics of this extracellular PGA are not known. The goal of this research was to characterize the extracellular PGA produced by B. subtilis BAC4. Enzyme production was carried out by batch fermentation, followed by enzyme purification and characterization of the PGA. The PGA activity was determined by the Kornfeld method, with optimal activity for hydrolysing penicillin G observed at 43 oC and pH 8.5. The activation energy of penicillin G hydrolysis by the PGA of B. subtilis BAC4 was determined as 4.9 kcal.mol-1 and Vmax and Km values were found to be 0.7 µmole.min-1.mg-1 and 3.5 mM respectively. PGA catalytic activity was competitively inhibited by phenylacetic acid with an inhibition constant, Ki(PAA) , of 347.2 mM. It was concluded that the extracellular PGA of B. subtilis BAC4 can hydrolyse penicillin G efficiently. Key words: PGA, extracellular, Bacillus, local
Co-Authors . WISJNUPRAPTO DEBBIE SOEFIE RETNONINGRUM Debbie Sofie Retnoningrum EDWAN KARDENA Endang Purwantini Hartiwi Diastuti Muhaimin Muhaimin OEI BAN LIANG Oei Ban Liang Pujawati Suryatmana Sadiah Achmad Sadijah Achmad Supartono -