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All Journal Jurnal Pengolahan Hasil Perikanan Indonesia Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati Jurnal Pascapanen dan Bioteknologi Kelautan dan Perikanan Journal of Marine and Coastal Science
safrina dyah hardiningtyas
Departemen Teknologi Hasil Perairan, Fakultas Perikanan Dan Ilmu Kelautan, IPB University, Jalan Agatis, Kampus IPB Dramaga, Bogor, 16680, (0251) 8622915
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Environmental Science Immunology & microbiology

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Journal : Jurnal Pengolahan Hasil Perikanan Indonesia

 1 
AKTIVITAS ANTIOKSIDAN DAN EFEK HEPATOPROTEKTIF DAUN BAKAU API-API PUTIH safrina dyah hardiningtyas; sri - purwaningsih; Ekowati - Handharyani
Jurnal Pengolahan Hasil Perikanan Indonesia Vol 17 No 1 (2014): Jurnal Pengolahan Hasil Perikanan Indonesia
Publisher : Department of Aquatic Product Technology IPB University in collaboration with Masyarakat Pengolahan Hasil Perikanan Indonesia (MPHPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (3054.319 KB) | DOI: 10.17844/jphpi.v17i1.8140

Abstract

Penelitian ini bertujuan untuk mendapatkan ekstrak kasar daun api-api putih yang memiliki aktivitas antioksidan terbaik, menentukan kandungan fitokimia dan efek Hepatoprotektif ekstrak kasar yang dipilih secara in vivo. Daun api-api putih diekstrak menggunakan tiga jenis pelarut, yakni metanol, etil asetat dan n-heksana. Dalam uji in vivo dilakukan evaluasi terhadap kadar malondialdehid (MDA), enzim aspartat transaminase (AST), enzim alanin transaminase (ALT), dan histopatologi. Hasil penelitian menunjukkan bahwa ekstraksi daun api-api putih dengan pelarut etil asetat menghasilkan aktivitas antioksidan terbaik dengan kandungan fitokimia terdiri dari flavonoid dan steroid/triterpenoid. Pemberian ekstrak etil asetat daun api-api putih pada tikus yang telah diinduksi CCl4 dapat menormalkan berbagai parameter biokimia stres oksidatif (kadar MDA, AST, dan ALT) dibandingkan dengan standar Silymarin. Ekstrak daun api-api putih berpotensi untuk melindungi hati tikus dari kerusakan oksidatif yang diinduksi CCl4. Efek Hepatoprotektif ekstrak daun api-api putih berkorelasi dengan aktivitas antioksidannya.Kata kunci: antioksidan, Avicennia marina, ekstrak, hepatoprotektor
Depolimerisasi Kitosan Menggunakan Sinar Ultraviolet dan Katalis Asam Klorida: Depolymerization of chitosan with ultraviolet ray and chloride acid catalyst Devi Faustine; Iriani Setyaningsih; Safrina Dyah Hardiningtyas
Jurnal Pengolahan Hasil Perikanan Indonesia Vol 23 No 3 (2020): Jurnal Pengolahan Hasil Perikanan Indonesia 23(3)
Publisher : Masyarakat Pengolahan Hasil Perikanan Indonesia (MPHPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17844/jphpi.v23i3.31656

Abstract

Chitosan is a natural product of chitin deacetylation which has a long molecular chain and high molecular weight, this makes chitosan constrained in the process of its application. To overcome this, it is necessary to do a depolymerization process that can reduce the molecular weight of chitosan and shorten the molecular chain of chitosan. Chitosan depolymerization in this study was carried out using ultraviolet radiation and strong acids to use chitosan polymer chains to produce chitosan with shorter chains and can be used in water or neutral solvents. Depolymerization begins with dissolving chitosan with hydrochloric acid solution, then irradiated with ultraviolet light for 45 minutes, 60 minutes, 75 minutes. Chitosan solution was precipitated using isopropyl alcohol, and filtered with 40 mesh nylon cloth. Chitosan which has a pH of seven is dried to get chitosan dissolved air. The air soluble chitosan obtained was analyzed by comparing each given administration and chitosan control. The use of ultraviolet light and HCl solutions designed to depolymerize the characteristics of chitosan produced. Selected based on chitosan depolymerized with 75 minutes of ultraviolet light irradiation with yield of 70.07±14.00%, solubility 99.80±0.17%, white degree 99.99±0.00%, viscosity 49.78±0.31 cP, molecular weight 169.46±0.30 kDa, and zeta potential 73.03±1.92 mV. The decrease in molecular weight and viscosity on the characteristics of water-soluble chitosan shows that the process of chitosan depolymerization occurs when the depolymerization effect increases with the duration of ultraviolet light irradiation.
Pengaruh Jenis Pelarut dan Ultrasonikasi terhadap Ekstrak Fikoeritrin dari Kappaphycus alvarezii : The Effect of Different Solvent Type on Phycoerithryn Extract from Kappaphycus alvarezii Anne Mumtaza Putri; Uju; Safrina Dyah Hardiningtyas
Jurnal Pengolahan Hasil Perikanan Indonesia Vol 24 No 2 (2021): Jurnal Pengolahan Hasil Perikanan Indonesia 24(2)
Publisher : Department of Aquatic Product Technology IPB University in collaboration with Masyarakat Pengolahan Hasil Perikanan Indonesia (MPHPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17844/jphpi.v24i2.37182

Abstract

Kappaphycus alvarezii is a type of red seaweed (Rhodophyta) which contains phycobiliprotein pigments. Red seaweed is dominated by phycoerythrin type phycobiliprotein. Phycoerythrin can be obtained from the extraction process. The purpose of this study was to determine the effect of solvent type and acceleration time of ultrasonication extraction on phycoerythrin pigments. The extraction process was carried out in stages using distilled water, CaCl2 and phosphate buffer with ultrasonication acceleration of 35-55 minutes. The results showed that the phycoerythrin pigment extract of K. alvarezii using distilled water produced a more concentrated pigment color than buffer phosphate and CaCl2, showing the highest concentration of 0.057 mg/mL, and a purity index of 0.189. Water solvent is safe to use because water does not have toxic power. So, there is no need to conduct a preliminary test for solvent toxicity. It is more economical than other types of solvents. The extraction of phycoerythrin pigment using distilled water for 45 minutes resulted in a more concentrated pigment color. The concentration of phycoerythrin pigment before and after precipitation was 0.170 mg/mL and 0.421 mg/mL, respectively. The phycoerythrin pigment purity index before and after precipitation was 0.301 and 0.831, respectively. The best antioxidant activity (% inhibition) was found at concentrations of 50 ppm and 80 ppm phycoerythrin pigment with extraction time of 45 minutes at 62% and 89% respectively. Protein profile using SDS-Page showed that phycoerythrin has a molecular weight of 16.27 kDa in the subunit, 17.79 kDa in the subunit β, and 30.60 kDa in the subunit γ.
Depolimerisasi Kitosan dari Cangkang Udang dengan Enzim Papain dan Iradiasi Sinar Ultraviolet: Depolymerization of Chitosan from Shrimp Shell Using Papain Enzyme and Ultraviolet Light Irradiation Rizfi Fariz Pari; Dewi Mayangsari; Safrina Dyah Hardiningtyas
Jurnal Pengolahan Hasil Perikanan Indonesia Vol 25 No 1 (2022): Jurnal Pengolahan Hasil Perikanan Indonesia 25(1)
Publisher : Masyarakat Pengolahan Hasil Perikanan Indonesia (MPHPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17844/jphpi.v25i1.40311

Abstract

Chitosan is a natural polymer with positive charge that is good to be applied in the pharmaceutical field as a drug carrier. However, commercial chitosan has a large molecular weight and low degree of deacetylation, so its application is limited due to its low solubility in water. This study aims to determine the effect of the depolymerization method of two classes of chitosan on the market. The types of chitosan used to produce chitooligosaccharide are pharmaceutical grade and food grade. The method used were UV-C light irradiation (75 minutes), papain enzyme hydrolysis (15 g/L for 16 hours, 40oC and pH 5) and a combination of UV and enzymes. The results showed that the grade of chitosan and the depolymerization method affected the characteristics of the product. The three methods used in pharmaceutical grade chitosan produce chitooligosaccharide which dissolved directly in water with a neutral pH. Chitooligosaccharide had yield of >62%, whiteness degree of 99.33%, molecular weight <5.5 KDa, and degree of deacetylation >85%. Food grade chitosan which has been depolymerized by different methods cannot be dissolved in water with a neutral pH. Food grade chitosan through the combination of UV+enzyme depolymerization has the same deacetylation quality as pharmaceutical grade chitosan.
Ekstraksi Polisakarida Ulvan dari Rumput Laut Ulva lactuca Berbantu Gelombang Ultrasonik pada Suhu Rendah : Ultrasonic Wave Assisted Extraction of Ulvan Polysaccharide from Ulva Seaweed at Low Temperature Wahyu Ramadhan; Uju Uju; Safrina Dyah Hardiningtyas; Rizfi Fariz Pari; Nurhayati Nurhayati; Devani Sevica
Jurnal Pengolahan Hasil Perikanan Indonesia Vol 25 No 1 (2022): Jurnal Pengolahan Hasil Perikanan Indonesia 25(1)
Publisher : Masyarakat Pengolahan Hasil Perikanan Indonesia (MPHPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17844/jphpi.v25i1.40407

Abstract

Ulvan merupakan polisakarida bersulfat utama dari rumput laut Ulva yang memiliki aktivitas biologis penting. Umumnya ulvan diekstrak menggunakan suhu tinggi dan dengan rentang waktu yang lama, oleh karena itu diperlukan metode ekstraksi alternatif yang dapat menghasilkan ulvan yang memiliki kualitas yang baik namun tetap mendukung konsep efisiensi energi. Salah satu metode green extraction yang dapat mengurangi penggunaan pelarut organik dan energi yang tinggi selama ekstraksi adalah dengan bantuan gelombang ultrasonik. Tujuan penelitian ini adalah untuk menentukan waktu, suhu dan jenis pelarut dengan bantuan ultrasonik yang paling baik dalam menghasilkan rendemen dan karakteristik ulvan yang sesuai dibandingkan dengan ulvan yang diekstraksi dengan metode konvensional atau tanpa bantuan ultrasonikasi. Pada penelitian tahap awal digunakan kombinasi antara jenis pelarut (akuades, HCl dan NaOH) dengan kondisi ekstraksi suhu rendah (50, 60, dan 70℃) selama 45 dan 90 menit dengan bantuan ultrasonik. Rendemen tertinggi ulvan diperoleh dari ekstraksi menggunakan NaOH pada suhu 70℃ selama 90 menit dengan bantuan ultrasonik sebesar 16,90±0,45%. molekul dan viskositas ulvan yang dihasilkan dengan berbantu ultrasonik menunjukkan penurunan nilai dibandingkan dengan tanpa ultrasonikasi. Hasil Fourier-transform infrared spectroscopy mengkonfirmasi pita serapan grup sulfat yang merupakan residu gula rhamnose pada panjang gelombang 1125 cm-1 dan C-O-S pada 983 cm-1. Ulvan yang diekstrak dengan sonikasi menunjukkan kandungan sulfat 39, 58 dan 53% untuk masing- masing pelarut akuades, HCl dan NaOH. Perlakuan ultrasonikasi memberikan pengaruh pada kandungan sulfat 2-14% lebih tinggi dibandingkan dengan sulfat yang dihasilkan dari metode konvensional.
Kinerja Pembangkit Biolistrik Salt Bridge Mirrobial Fuel Cell Variasi Rasio Karagenan-Karboksimetil Selulosa: The Performance of Salt Bridge Microbial Fuel Cell Bioelectric Generator in Variated Carrageenan-Carboxylmethyl Cellulose Ratio Bustami Ibrahim; Safrina Dyah Hardiningtyas; Steffen
Jurnal Pengolahan Hasil Perikanan Indonesia Vol 25 No 2 (2022): Jurnal Pengolahan Hasil Perikanan Indonesia 25(2)
Publisher : Masyarakat Pengolahan Hasil Perikanan Indonesia (MPHPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17844/jphpi.v25i2.39872

Abstract

Sistem microbial fuel cell (MFC) adalah teknologi terbarukan yang dapat mengubah materi organik menjadi energi berupa listrik. Tujuan penelitian ini menentukan rasio karagenan (K) : karboksimetil selulosa (KMS) yang optimal pada jembatan garam untuk menghasilkan energi listrik dalam sistem MFC dan menentukan hasil listrik tertinggi dari limbah cair pindang ikan melalui teknologi MFC salt bridge. Jembatan garam K:KMS dibuat dengan perlakuan rasio K:KMS 1:1, 0,5:1, 0,6:1 (b/b). Perlakuan terbaik adalah K:KMS dengan perbandingan 1:1 menghasilkan tegangan listrik tertinggi sebesar 0,88 V. Jembatan garam K:KMS dengan perbandingan 0,5:1 menghasilkan kuat arus tertinggi sebesar 1,22 mA, serta daya listrik tertinggi sebesar 0,85 mW. Nilai efisiensi penurunan BOD, dan TAN mengalami penurunan sebesar 90,36%, dan 60,45% pada perlakuan rasio K:KMS 1:1. Sistem salt bridge MFC ini menunjukkan kinerja yang sangat baik dan berpotensi untuk dikembangkan di masa depan.
Co-Authors Anne Mumtaza Putri Bustami Ibrahim Dedi Soedarma Devani Sevica Devi Faustine Dewi Mayangsari Dona Fradilla Bahri Ekowati Handharyani Hefni Effendi MUJIZAT KAWAROE Nurhayati Nurhayati Pipih Suptijah Ranula Supraba Rizfi Fariz Pari Rizfi Fariz Pari Sri Purwaningsih Steffen TATI NURHAYATI Uju Uju Uju Wahyu Ramadhan