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All Journal Jurnal Gizi dan Pangan Jurnal Ilmu Pertanian Indonesia ACTA VETERINARIA INDONESIANA Jurnal Pengolahan Hasil Perikanan Indonesia Hemera Zoa Jurnal Ilmu Komputer dan Informasi Jurnal Veteriner Majalah Kedokteran Bandung Jurnal Ilmu Ternak dan Veteriner INDONESIAN JOURNAL OF PHARMACY Pharmascience Jurnal Kedokteran Gigi Universitas Padjadjaran Jurnal Medik Veteriner Jurnal Pascapanen dan Bioteknologi Kelautan dan Perikanan Jurnal Farmamedika (Pharmamedica Journal) Jurnal Dunia Gizi Jurnal Kedokteran Hewan

Ekowati Handharyani

Department Of Clinic, Reproduction And Pathology, Faculty Of Veterinary Medicine, IPB University, Bogor, Indonesia

Author-ID : 274303

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Computer Science & IT Dentistry Environmental Science Health Professions Immunology & microbiology Medicine & Pharmacology Public Health Veterinary

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Extrahepatic bile duct ligation in broiler chickens: ultrastructural study of Ito cell Handharyani, Ekowati; Ochiai, K; Winarto, A
Indonesian Journal of Animal and Veterinary Sciences Vol 9, No 4 (2004)
Publisher : Indonesian Animal Sciences Society

The Ito cell (fat-storing cell) is a cell lying in perisinusoidal space of liver. The function of Ito cell is expanding from a site of fat-storing site to a center of extracellular matrix metabolism and mediator production in the liver. This study was performed in order to evaluate the Ito cells in cholestatic condition. The artificial cholestatic was conducted by ligation of extrahepatic bile ducts (bile duct ligation = BDL) in broilers. The results showed that BDL induced bile congestion, fibrosis, proliferation of Ito cells and intrahepatic bile ductules. Immunohistochemistry demonstrated that Ito cells were scattered throughout the fibrotic areas, and larger in size with more extensive immunoreactivity than those in normal livers. Ultrastructural study demonstrated that Ito cells were closely associated with the production of extracellular collagen fibers. Ito cells actively react against hepatocytic injuries, especially in fibrogenesis of cholestatic livers. Â Key words: Bile duct, ito cell, broiler

Seroprevalence of Q fever in sheep and goat in West Java area Setiyono, Agus; Handharyani, Ekowati; Mahatmi, Hapsari
Indonesian Journal of Animal and Veterinary Sciences Vol 13, No 1 (2008)
Publisher : Indonesian Animal Sciences Society

Q fever is a zoonotic disease caused by Coxiella burnetii, a species of bacteria that is distributed globally. Ruminant especially sheep and goats may play an important role in the transmission of the disease to human. The research of seroprevalence of Q fever in sheep and goats was carried out from August 2006 to March 2007 in West Java area. A total of 138 sera were collected; 69 sera from sheep and 69 sera from goats. The indirect immunoflourescent antibody test was used to determine the seroprevalence of Q fever. The seropositive based on the dilution of serum starting from 1 : 16. Seropositive were observed in 22 samples (31.88%) of sheep and 14 samples (20.28%) of goats. The highest titer of 1 : 128 was observed in 3 pregnant sheep. The results of the present study suggested that Q fever was endemic in West Java area. Key Words: Q Fever, Prevalence, Indirect Immunoflourescent Antibody Test

Pemberian Asam Valproat pada Induk Tikus Bunting Menghambat Sintesis Insulin pada Sel Otak Anak Tikus Komariah, Komariah; Kiranadi, Bambang; Winanto, Adi; Manalu, Wasmen; Handharyani, Ekowati
Majalah Kedokteran Bandung Vol 49, No 3 (2017)
Publisher : Faculty of Medicine, Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15395/mkb.v49n3.1119

Asam valproat memengaruhi aktivitas histone deacetylase yang berperan dalam ekspresi gen selama organogenesis. Insulin berperan dalam proliferasi dan diferensiasi sel-sel saraf dentate gyrus hipokampus. Penelitian ini bertujuan mempelajari pengaruh pemaparan asam valproat pada induk bunting terhadap ekspresi gen insulin pada dentate gyrus. Penelitian dilakukan di UPHL IPB pada bulan Mei 2015 hingga Desember 2016 denganÂ 84 ekor anak tikus yang dilahirkan oleh induk tikus kontrol yang diberi asam valproat 250 mg pada umur kebuntingan 10, 13, dan 16 hari digunakan untuk pengamatan kadar glukosa, insulin, DNA, RNA, dan rasio RNA/DNA serta pengamatan mikroskopis otak. Pengamatan dilakukan selang waktu empat minggu, dimulai dari umur 4 sampai 32 minggu. Anak tikus yang dilahirkan oleh induk tikus yang diberi asam valproat selama kebuntingan mempunyai kadar glukosa otak yang lebih tinggi (p<0,01) dan insulin yang lebih rendah (p<0,05).Â Selama periode pertumbuhan, anak tikus yang dilahirkan oleh induk tikus yang diberi asam valproat mengalami peningkatan kadar glukosa dan penurunan kadar insulin (p<0.05). Pengamatan mikroskopis sel-sel dentate gyrus menunjukkan degenerasi sel dan tidak terlihat reaksi imunoreaktif terhadap insulin, namun terjadi penurunan konsentrasi DNA, RNA, serta rasio RNA/DNA (p<0,05). Pemberian asam valproat pada induk tikus pada umur kebuntingan 10, 13, dan 16 hari memengaruhi organogenesis otak anak tikus sehingga menyebabkan kerusakan sel-sel saraf penghasil insulin otak yang ditunjukkan olehÂ penurunan sekresi dan kadar insulin. [MKB. 2017;49(3):156â€“64]Kata kunci: Asam valproat, dentate gyrus, insulin, organogenesisÂ Valproic Acid Administration in Pregnant Rats Inhibits Insulin Synthesis n in Brain Cells of the OffspringsValproic acid affects the activity of histone deacetylase which eventually affects gene expression during organogenesis. Insulin plays a role in regulating the proliferation and differentiation of the dentate gyrus. This experiment was designed to study the effects of valproic acid administration on the expression of insulin gene in the dentate gyrus of pregnant rats.Â This study was carried out at the Laboratory Animal Management Unit (UPHL) of Bogor Agricultural UniversityÂ from May 2015 to December 2016, with eighty four newborn rats from control rats and VA-administered rats at the age of 10, 13, and 16 days of pregnancy. The measurements were done for glucose, insulin, DNA, and RNA concentrations in dentate gyrus and to observe the microscopic condition of the brain at the ages of 4 to 32 weeks with 4 weeks interval.Â Rats born form mothers administered by 250 mg valproic acid during pregnancy had higher glucose concentrations (p<0.01) and lower insulin concentrations (p<0.05) in the dentate gyrus. During the post-natal growth phase, rats born from mothers administered with valproic acid had increased glucose concentrations and decreased insulin concentrations (p<0.05). Microscopic observations of the dentate gyrus showed degeneration of nerve cells with no immunoreaction to insulin was seen in dentate gyrus cells. However, the concentrations of DNA, RNA, and the ratio of RNA/DNA in the dentate gyrus cells decreased (p<0.05). The administration of valproic acid to pregnant rats at the age of 10, 13, and 16 days of pregnancy destructsÂ brain organogenesis of the offsprings which eventually disturbs insulin production in the dentate gyrus indicated by decreased insulin secretion and concentrations.Â [MKB. 2017;49(3):156â€“64]Key words: Dentate gyrus, insulin, organogenesis, valproic acid

Pengaruh Minyak Ikan Lele terhadap Kerusakan Sel Saraf, Sel Hepatosit, dan Sel Ginjal Tikus Sprague Dawley Firmansyah, Hardi; Kusharto, Clara Meliyanti; Roosita, Katrin; Handharyani, Ekowati; Rosmiati, Risti
Jurnal Dunia Gizi Vol 4, No 1 (2021): Edisi Juni
Publisher : Study Program of Nutrition, Public Health Faculty, Institut Kesehatan Helvetia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33085/jdg.v4i1.4749

Pendahuluan; Minyak ikan lele (Clarias gariepinus) mengandung omega 3 dan omega 6 yang dapat menurunkan kadar trigliserida dalam darah dan memberikan efek positif terhadap jaringan hati. Tujuan; untuk menganalisis pengaruh pemberian minyak ikan lele terhadap jumlah sel saraf, sel hapatosit dan sel ginjal yang rusak pada tikus galur Sprague Dawley yang diberi pakan hiperkolesterolemia. Bahan dan Metode; Desain penelitian ini Randomized Control trial (RCT) pada hewan coba tikus galur Sprague Dawley dengan empat perlakuan yaitu A1 (kontrol), A2 (minyak ikan lele), A3 (minyak ikan lele + omega 3) dan A4 (minyak ikan lele+omega 3+vitamin E). Sel-sel yang mengalami abnormalitas dihitung di bawah mikroskop cahaya (pembesaran 20x40) untuk kemudian dianalisis univariat (rata-rata±standar deviasi) serta analisis bivariat (one-way ANOVA). Hasil; Rata-rata jumlah sel yang mengalami kerusakan pada sel saraf di hipokampus pada kelompok tikus kontrol (A1) tidak berbeda signifikan dibandingkan semua kelompok tikus yang diberikan minyak ikan lele (A2, A3, A4) (p0,05). Hasil serupa juga ditunjukkan pada rara-rata jumlah sel hepatosit dan sel ginjal yang rusak. Kesimpulan; Pemberian minyak ikan lele dengan komposisi berbeda tidak memberikan perbedaan signifikan pada kerusakan sel saraf, sel hepatosit, dan sel ginjal tikus galur Sprague Dawley.

The effect of the extract of pasak bumi roots (Eurycoma longifoliaJack.) on liver function Panjaitan, Ruqiah Ganda Putri; Manalu, Wasmen; Handharyani, Ekowati; ., Chairul
Indonesian Journal of Pharmacy Vol 22 No 1, 2011
Publisher : Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia

The aim of this research is to study the effects of the methanol extract and its derived fraction (n-hexane, chloroform, ethyl acetate, and methanolwater) on liver function of male rats. The treatment groups were administered 500 mg/kg body weight of metanol extract and its derived fractions (n-hexane, chloroform, ethyl acetate, n-butanol, methanol-water) of E. longifolia for 7 consecutive days. Positive control group received 25 mg silymarin/kg body weight and negative control group received 2 mL aquadest/kg body weight daily for 7 consecutive days. Liver function was monitored by measuring alanine transaminase (ALT), aspartate transaminase (AST), total protein, alkaline phosphatase (ALP), total bilirubin, direct bilirubin, and indirect bilirubin consentrations in the serum. Compared with control, oral administration of methanol extract and derived fractions of methanol extract of E. longifolia root had no significant effects on liver function (p>0.05). Methanol-water fraction gave similar results to silymarin.Key words: Eurycoma longfolia Jack.,liver function, biochemical blood Histopathologi

IDENTIFIKASI KOMPONEN KIMIA DAN AKTIVITAS ANTIOKSIDAN DALAM TANAMAN TORBANGUN (Coleus amboinicus Lour) Trini Suryowati; Rimbawan Rimbawan; Rizal M Damanik; Maria Bintang; Ekowati Handharyani
Jurnal Gizi dan Pangan Vol. 10 No. 3 (2015)
Publisher : Food and Nutrition Society of Indonesia

ABSTRACTThe objective of this study was to determine the chemical compound in leaves, stem, and root by GC-MS technique and antioxidant activity of torbangun leaves (Coleus amboinicus Lour). The torbangun leaves ethanol extract were tested to antioxidant activity assay using DPPH, and á-glucosidase inhibitory effects was measured with a spectrophotometric method. The analysis of leaves revealed the presence of Carbamic acid, monoammonium salt (CAS) Ammonium carbamate (11.73%), Hexadecanoic acid (CAS) Palmitic acid (8.35%), I-Limonene (5.92%), Heptadecene-(8)- carbonic acid-(1) (4.76%), Oxacycloheptadec-8-en-2-one (CAS) Ambrettolide (4.70%). The analysis of stem revealed the presence of Formamide (CAS) Methanamide (22.48%), 12,13- Dimethyl-2,7- dioxa 5,10 diazatricyclo [4.4.4.0(1,6)] trans -tetradecan-12 (13.22%), Hexadecanoic acid (CAS) Palmitic acid (11.51%), 2-Propanone, 1-hydroxy- (CAS) Acetol (10.14%), 9-Octadecen-1-ol, (Z)- (CAS) cis-9-Octadecen-1-ol (7.09%). The analysis of roots revealed the presence of Methanamine, N-methyl- (CAS) Dimethylamine (28.45%), Acetic acid (CAS) Ethylic acid (9.78%), 3.2-Propanone, 1-hydroxy- (CAS) Acetol (6.41%), 1-Propen-2-ol, acetate (CAS) Isopropenyl acetate (5.16%), 4.73 Phenol, 2-methoxy- (CAS) Guaiacol(4.73%). The DPPH result of torbangun leaves ethanol extract obtained by IC50 247,942 ppm and ascorbic acid standard was 1 ppm. IC50 values inhibition of á-glucosidase extract was >100 ppm and glucobay standard was 0.264 ppm. This research provided a chemical compound and the torbangun leaves ethanol extract capable of acting as antioxidant based on IC50 values.Keywords: antioxidant activity, chemical compound, Coleus amboinicus LourABSTRAKPenelitian ini bertujuan untuk mengetahui komponen senyawa kimia dalam daun, dahan, dan akar menggunakan analisis Kromatografi Gas-Spektrometri Massa (GC-MS) serta aktivitas antioksidan daun torbangun (Coleus amboinicus Lour). Daun torbangun yang diekstrak etanol dianalisis aktivitas antioksidan menggunakan test DPPH, dan daya hambat enzim á-glukosidase diukur dengan metode spektrofotometer. Hasil analisis dalam daun menunjukkan komponen kimia Carbamic acid, monoammonium salt (CAS) Ammonium carbamate (11,73%), Hexadecanoic acid (CAS) Palmitic acid (8,35%), I-Limonene (5,92%), Heptadecene-(8)-carbonic acid-(1) (4,76%), Oxacycloheptadec-8-en-2-one (CAS) Ambrettolide (4,70%). Hasil analisis dalam dahan menunjukkan komponen kimia Formamide (CAS) Methanamide (22,8%), 12,13-Dimethyl-2,7-dioxa5,10diaza tricyclo [4.4.4.0(1,6)] trans-tetradecan-12 (13,22%), Hexadecanoic acid (CAS) Palmitic acid (11,51%), 2-Propanone,1-hydroxy-(CAS) Acetol (10,14%), 9-Octadecen-1-ol, (Z)- (CAS) cis-9-Octadecen-1-ol (7,09%). Hasil analisis dalam akar menunjukkan komponen kimia Methanamine, N-methyl-(CAS) Dimethylamine (28,45%), Acetic acid (CAS) Ethylic acid (9,78%), 3.2-Propanone, 1- hydroxy- (CAS) Acetol (6,41%), 1-Propen-2-ol, acetate (CAS) Isopropenyl acetate (5,16%), 4.73 Phenol, 2-methoxy- (CAS) Guaiacol (4,73%). Hasil uji antioksidan terhadap daun torbangun dengan metode DPPH didapatkan IC50 247,942 ppm dibandingkan standar vitamin C 1 ppm. Nilai IC50 dari penghambatan enzim á-glukosidase dalam ekstrak daun torbangun >100 ppm dibandingkan dengan standar glukobay 0,264 ppm. Hasil penelitian ini menunjukkan bahwa daun torbangun yang diekstrak dengan etanol mampu berperan sebagai antioksidan berdasarkan nilai IC50.Kata kunci: aktivitas antioksidan, Coleus amboinicus Lour, komponen kimia

Pemberian minyak ikan lele (Clarias gariepinus) terhadap bobot badan dan perubahan histopatologi hati, ginjal, dan otak tikus galur Sprague dawley yang diberi pakan hiperkolesterolemia Hardi Firmansyah; Katrin Roosita; Clara Meliyanti Kusharto; Ekowati Handharyani
Jurnal Gizi dan Pangan Vol. 12 No. 2 (2017)
Publisher : Food and Nutrition Society of Indonesia

The objective of this study was to analyze changes in body weight and cell morphology in liver, kidney and brain tissue of Sprague dawley rats administeredÂ hypercholesterolemia diets for four weeks. Four diet treatments were applied in this study: control, catfish oil, catfish oil with the addition of omega 3 and catfish oil with the addition of omega 3 and vitamin E. Four weeks after the intervention, the rats were sacrificed and the internal organs (liver, brain, and kidney) were taken for histological preparations by hematoxylin-eosin (HE) and immunohistochemistry test. The study showed that the catfish oil intervention tend to reduced weight gain of rats fed hypercholesterolemia diets. More lipid accumulation in liver of the control group was found than the intervention groups. However, there was no difference in the histopathology of the kidney and brain tissues between the control group and intervention groups. It is concluded that catfish oil study provides a positive effect in rat liver tissue, but no difference exists between the brain and kidney tissue. There is no defect found at hippocampus of rats by formed of amyloid plague.

Potential of okra (Abelmoschus esculentus L.) extract to reduce blood glucose and malondialdehyde (MDA) liver in streptozotocin-induced diabetic rats Putri Puncak Anjani; Evy Damayanthi; Rimbawan Rimbawan; Ekowati Handharyani
Jurnal Gizi dan Pangan Vol. 13 No. 1 (2018)
Publisher : Food and Nutrition Society of Indonesia

The study aimed to analyze the potential of okra to reduction blood glucose and MDA liver in streptozotocin (STZ)-induced diabetic rats. Design experiment used in this study was pre and post test controlled group design. The first step of this study was analyzed bioactive compound of okra extract. The next step was administired orally of okra extract to control and diabetic rats induced by streptozotocin 50 mg/kgBW for 14 days. Sprague dawley rats were divided into six groups: normal control (N), diabetic control (DM), diabetic treated with green okra extract (GOE) with the dosage of 5 mg/kgBW quercetin and 10 mg/kgBW quercetin, diabetes treated with purple okra extract (POE) with the dosage of 5 mg/kgBW quercetin and 10 mg/kgBW quercetin. Blood glucose were measured each five days and malondialdehyde (MDA) liver in rats were measured in the end of intervention. The following results show that phenolic and quercetin of purple okra extract were higher (3.60%; 0.45 mg/g) than green okra extract (3.58%; 0.27 mg/g). Administration of GOE I, GOE II, POE I and POE II in diabetic rats showed significant (P<0.05) reduction in blood glucose level (115.25 mg/dl; 86 mg/dl; 180.75 mg/dl; 91 mg/dl) and improve level of MDA. These results suggest that intervention of okra extract based on quercetin compound show an antihyperglicemic potential and improve MDA level.

Serologi dan virologi virus avian influenza H5N1 pada kucing jalanan di Kota Bogor Sri Murtini; R. Susanti; Ekowati Handharyani
Jurnal Ilmu Pertanian Indonesia Vol. 14 No. 1 (2009): Jurnal Ilmu Pertanian Indonesia
Publisher : Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1426.848 KB)

Highly pathogenic avian influenza (HPAI) H5N1 virus is a known pathogen in birds. Recently, the virus has been reported to cause sporadic fatal disease in tigers, leopards, and other exotic felids as well as domestic cats in Thailand. The present study was carried out to investigate the presence of AI H5N1 virus infection in stray cats roaming around residential, traditional and chicken farms in Bogor, West Java. Ninety serum samples were tested using HI test to screened for the presence of antibody to AI H5N1. Virus isolation was done in SPF embrionated chicken eggs and identify using HI, AGP and RT-PCR. The results showed that 18,9% of stray cats developed antibodies against H5 with geometric mean titre 23,1 . Stray cats lived in traditional markets 18–40% developed antibodies in the titre ranging from 22,8 to 24,5. Only two out of nine stray cats which lived in chicken farm developed low antibody titres again H5 (21). None of the stray cats lived in residencial area have developed antibodies against H5. This study revealed that stray cats have been contact with AI H5. Avian influenza H5 viruses were isolated in eight out of 33 pooled of rectal swab samples. The viral cleavage site sequences are CCTCAAAGAGAGAGC AGAAGAAAGAAGAGAGGT which represent amino acid sequences of PQRESRRKKRG. Based on the cleavage site sequence, the isolates are similar with the AI H5 virus subtype isolated from human in Indonesia during 2005–2007.

Lesio Aterosklerosis pada Arteri Iliak Monyet Ekor Panjang sebagai Hewan Model Peripheral Arterial Disease (PAD) Sri Rahmatul Laila; Dewi Apri Astuti; Irma Herawati Suparto; Ekowati Handharyani; Dondin Sajuthi
Acta VETERINARIA Indonesiana Vol. 7 No. 1 (2019): Januari 2019
Publisher : IPB University

Aterosklerosis pada arteri iliak berkaitan dengan kejadian peripheral arterial disease (PAD) pada manusia. Penelitian ini bertujuan untuk menganalisis lesio aterosklerosis pada arteri iliak sebagai salah satu faktor penyebab PAD. Penelitian ini menggunakan sembilan monyet ekor panjang jantan dewasa dari 3 kelompok respons yang diberi pakan aterogenik IPB-1 selama 2 tahun. Arteri common iliak diambil pada saat nekropsi, diproses menjadi slaid histologi dan diwarnai dengan Verhoeff van Giesson (VVG). Keparahan lesio dikategorikan sebagai grade I sampai VI dan dianalisis secara deskriptif. Area lesio ditentukan melalui panjang lamina elastik interna (PLEI), area intima (AI), serta ketebalan maksimum intima (KIM) dan dianalisis dengan ANOVA. Hasil penelitian menunjukkan 55,56% monyet membentuk lesio plak aterosklerosis (grade IV sampai VI) yang mana mereka berasal dari kelompok intermediet dan hiper-responsif. Kelompok hiper-responsif menunjukkan AI dan KMI paling tinggi (0,979 mm2; 0,352 mm) diikuti kelompok intermediet (0,372 mm2; 0,237 mm) dan kelompok hipo-responsif (0,049 mm2; 0,052 mm) (P<0,05). Hasil ini menunjukkan semakin tinggi kolesterol plasma, semakin luas area lesio yang terbentuk. Keseluruhan hasil mendukung bahwa monyet yang diberi pakan aterogenik IPB-1 selama 2 tahun mampu membentuk lesio plak aterosklerosis pada arteri common iliak, dan berpotensi sebagai hewan model PAD, kecuali kelompok hipo-responsif.

Co-Authors ., Chairul A Winarto Adi Winanto Adi Winarto Agus Buono Agus Setiyono Akhmad Endang Zainal Hasan Aziz Kustiyo Bambang Kiranadi Bambang Sumiarto Bambang Widyo Prastowo BIBIANA W LAY Chairul - Chairul Chairul Clara Meliyanti Kusharto Clara Meliyanti Kusharto DENI NOVIANA DEWI APRI ASTUTI Dewi Ratih Agungpriyono Dhany Nugraha Ramdhany Dondin Sajuthi Eflisa Endah Putri Elly Munadziroh Erni Sulistiawati Erwin Erwin Etriwati E Evy Damayanthi Geneva Ariesta Gunanti . Handayu Untari Hapsari Mahatmi Hardi Firmansyah Hernomoadi . Hernomoadi Hernomoadi Irma Herawati Suparto K Ochiai Katrin Roosita Kiranadi Bambang Komariah Komariah Komariah Lili Andriani M.B. Malole . Maria Bintang Masdiana C. Padaga Masriani . Ni Luh Putu Ika Mayasari Novera Nirmalasanti Octarina Octarina Okti Nadia Poetri Putri Puncak Anjani R. Susanti Retno Damajanti Soejoedono Retno Damayanti Soejoedono Rimbawan , Risti Rosmiati Rizal M Damanik Ruqiah Ganda Putri Panjaitan safrina dyah hardiningtyas Sangko Sayuti Nasution Sophia Setyawati sri murtini . Sri Purwaningsih Sri Rahmatul Laila Sri Wahyuni Surachmi Setiyaningsih Tanjung Penataseputro Trini Suryowati Wasmen Manalu Wiwin Winarsih Yuli Purwandari Kristiangingrum Yuni Andriani Yusuf Ridwan Zulfa Zakiah

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