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Journal : Proceedings of Annual International Conference Syiah Kuala University - Life Sciences

Characteristics of frozen-thawed semen on Simmental and Limousin bulls in Ungaran, Indonesia Triva Murtina Lubis; Prabowo Purwono Putro; Aris Junaidi; . Hamny
Proceedings of The Annual International Conference, Syiah Kuala University - Life Sciences & Engineering Chapter Vol 1, No 1 (2011): Life Sciences
Publisher : Syiah Kuala University

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Abstract

The present research aimed to study the characteristics of frozen-thawed semen in beef bulls ex-import in Ungaran, Indonesia. 5 heads Simmental and 5 heads Limousin of 5.5 years old were used in this research. The research was done in Ungaran Artificial Insemination Center, Central Java, Indonesia and Laboratory of Reproduction and Obstetry, Faculty of Veterinary Medicine, Gadjah Mada University, Yogyakarta, Indonesia. Frozen semen was obtained from this AI center. Results of the research indicated that the average individual motility of Simmental and Limousin is 39.00±5.48% and 36.00±2.24%. The average percentage of live spermatozoa is 54.00±10.75% for Simmental and 48.20±9.78% for Limousin while  the average proportion of abnormal spermatozoa is 9.60±3.36% and 6.80±4.15% for both Simmental and Limousin, respectively. On average, Simmental frozen-thawed semen have the higher mean proportion of sperm motility, live sperm, and abnormal sperm than that of Limousin frozen-thawed semen
The traits of fresh and frozen semen on brahman bulls Triva Murtina Lubis; Prabowo Purwono Putro; Aris Junaidi; Sumarti Suryaningsih
Proceedings of The Annual International Conference, Syiah Kuala University - Life Sciences & Engineering Chapter Vol 2, No 1 (2012): Life Sciences
Publisher : Syiah Kuala University

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Abstract

The present study aimed to determine quality and parameters traits of both fresh and frozen semen in 4-year old Brahman bulls. Semen was collected from 5 bulls twice a week using artificial vagina in Ungaran ArtificialInsemination Center, Central Java, Indonesia. Immediately after collection, samples were evaluated for semen volume, color, odor, viscosity, mass movement, individual movement, spermatozoa concentration and livespermatozoa percentage. Sperm concentration was measured using a spectrophotometer. Good quality fresh semen was processed to be frozen semen. Parameters evaluated for frozen semen include spermatozoa individualmovement, live spermatozoa, and abnormal spermatozoa percentage. The results showed that the semen volume is 6.90 ± 0.54 whereas the average of sperm concentration, individual motility and live sperm are 1754.54 ± 212.67;65.50 ± 3.74% and 76.30 ± 2.64%, respectively. Meanwhile, for frozen semen, the average percentage of motility is 38.00 ± 2.74% while the average proportions of live sperm and abnormal sperm are respectively 45.20 ± 9.86% and 6.00 ± 2.83%. In conclusion, in average, all parameters of fresh and frozen semen of Brahman bulls are in normal range. Based on the Indonesian National Standard, frozen semen produced could be used for insemination