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All Journal Jurnal Sain Veteriner Buletin Peternakan Jurnal Veteriner Jurnal Ilmu Ternak Veteriner Indonesian Journal of Biotechnology Journal of Health (JoH) Jurnal Kedokteran Hewan

Asmarani Kusumawati

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Author-ID : 235070

Biochemistry, Genetics & Molecular Biology Health Professions Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Nursing Public Health Veterinary Other

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Serotype detection, molecular characterization and genetic relationship study on Pasteurella multocide local isolate Sri Suryatmiati Prihandani; Susan M Noor; Asmarani Kusumawati
Jurnal Ilmu Ternak dan Veteriner Vol 22, No 2 (2017): JUNE 2017
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Pasteurella multocide is a bacteria that causes snoring disease or Haemorrhagic Septicaemia (HS) in Indonesia with high mortality and morbidity in heterogeneous species including cattle as a source of animal products with high economic value. The complexity of conventional and biochemical identification is a major obstacle in the detection of this disease because P. multocide has five serotypes A, B, D, E and F, while serotype B is the leading cause of HS cases in Asia including Indonesia. Therefore, it is necessary to conduct a research that can determine the serotype and molecular characterization and genetic study of five isolates of P. multocide from Lampung and Kupang by Polymerase Chain Reaction (PCR) technique. After PCR was performed on specific genes, capsular genes, 16S rRNA genes, sequencing and analysis using Bioedit, BLASTn, CLUSTALW and MEGA7.0.25, it was found that the five isolates were divided into two serotype groups: A and B. Isolate P. multocide (code: PMc) from Lampung is high homolog with ATCC isolate 12945, so it can be used as a positive control serotype A in the detection of other P. multocide isolates with PCR. Whereas, isolate P. multocide from Kupang can be used as positive control of serotype B because it is identical to P. multocide PMTB2.1 (CP007205.2) from Malaysia that is isolated from buffalo infected by HS.

Cortisol and Estradiol Profile in Cross-bred Ettawa Does: The Effects of Body Condition Scoring (BCS). Puji Astuti; D. Tri Widayati; S. Sunendar; K. Suharto; Asmarani Kusumawati; A. Junaidi
Indonesian Journal of Biotechnology Vol 13, No 1 (2008)
Publisher : Universitas Gadjah Mada

Body Condition Scoring (BCS) is an estimation of the muscle and fat development of an animal. Thin ewes that are fighting to maintain their own body weight and low concentration of cortisol are not able to ovulate as ewes in a more desirable condition due to lack of oestradiol concentration. The aims of this research are to monitor the cortisol and oestradiol profile in Cross-bred ettawa does and to determine effect of BCS on the cortisol and oestradiol profile. Eight does were used in this research. These animals were devided equally into 2 groups based on Body Condition Scoring (BCS), namely BCS 2, which body weight range between 25-30 kgs as group I ( >n=4 ) and BCS 3 which consists of ettawa with body weight range between 33-40 kg as group II ( n=4 ). All animals were synchronized using implant of CIDR and PGF2alpha. Blood from jugular vein were collected every 3 and 6 hours as soon as oestrus until 72 hours. Serum contained cortisol and oestradiol then assayed using ELISA</div><div>method. Cortisol and oestradiol concentrations were compared between groups by T test. The results showed that average concentration of cortisol is 47.17 ± 42.19 ng/mL for BCS 2 and 112.40±74.41ng/mL for BCS 3 (P<0.05), whereas concentration of oestradiol is 72.25±30.62 pg/mL for BCS 2 and 145.72±100.18 pg/mL for BCS 3 (P<0.05). Either cortisol or oestradiol have very synchronized wave except 2 of animals from BCS 2 (50%), which has tendency to suppress each other. It was concluded that profile of cortisol and oestradiol hormone have a very similar pattern, and BCS can affect hormone profile.

Characterization of envelope-transmembrane Gene of Jembrana Disease Virus Tabanan 1995 Isolate Asmarani Kusumawati; Rarastoeti Pratiwi; Pudji Astuti; Penny Humaidah Hamid
Indonesian Journal of Biotechnology Vol 15, No 1 (2010)
Publisher : Universitas Gadjah Mada

The availability of specific and rapid detection methods is essential for monitoring the health status of farmed species, particularly in viral disease as in this case early diagnosis is a critical factor in containing disease outbreaks. Jembrana Disease Virus (JDV) is a lentivirus that causes an acute, severe disease syndrome in infected Bali cattle in Indonesia, resulting in heavy economic losses because of the high mortalities. The virus-host interaction and the modes of transmission are still unknown. The goal of the research was to designa probe candidate of Jembrana Disease Virus based on envelope-transmembrane (env-tm) gene to optimize Jembrana disease detection method. The DNA fragment derived from env-tm of JDV was used, cloned in pGEX-TM and expressed in E.coli DH 5α. Sequence analysis was conducted with BLAST programs from NCBI. Sequence analyses of the fragments of env-tm clone, indicated that it has a very closed genetic relation with 97,68% homology identity. Probe was designed based on the conserved region of env-tm using Geneious resulted in JT2 252 bp long. BLAST analyses showed that probes had high specifity to other strains of JDV in Indonesia.Key words : probe, env-tm, JDV, specifity, sensitivity.

DIGoxigenin (DIG) Labeled Probe Candidate of Surface Antigen 1 (SAG1) for Toxoplasma gondii Detection Asmarani Kusumawati; Nafratilova Septiana; Sri Hartati
Indonesian Journal of Biotechnology Vol 16, No 1 (2011)
Publisher : Universitas Gadjah Mada

Toxoplasma gondii is one of the opportunistic pathogen that causes toxoplasmosis. Infection of Toxoplasma gondii has been estimated as high both in human and animal. The manifestation of infection were abortion, hydrocephalus, brain calcification, chorioretinal scar, and loss of productivity even to death in patients with acquired immunosuppression. Early diagnostic method which are rapid and accurate is essential for T. gondii detection because of its high prevalence. The purpose of this study was to develop a sensitive probes derived from Surface Antigen 1 (SAG1) for detection T. gondii and to examine the specificity and sensitivity of probe as diagnostic tool for toxoplasmosis. This research used SAG1 gene of T. gondii local isolate IS-1 that was cloned into pGEX-2T and transformed into Eschericia coli DH5α. The sequence of SAG1 was labeled with DIGoxigenin (non radioactive labeled) using PCR DIG Labeling Mix to derive 213 bp (probe-TS). BLAST and dot-blot hybridization analyses showed that probes had high specifity with other strains of T. gondii. Probe was able to detect T. gondii DNAup to 10 ng/μl of total sample DNA.

Penggunaan Protein Reporter GFP (Green Fluorecent Protein) untuk Pemantauan Fungsi Annexin I pada Phagositosis Brucellasuis Asmarani Kusumawati
Jurnal Sain Veteriner Vol 20, No 2 (2002): Desember
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

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Ekspresi Dan Isolasi Protein Rekombinan Surface Antigeni (Sagi) Toxoplasma GondII Isolat Lokal Pada Escherica Coli BL21 Sri Hartati; Asmarani Kusumawati; Hastari Wuryastuti; J.Sri Widada
Jurnal Sain Veteriner Vol 24, No 2 (2006): DESEMBER
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

CONSTRUCTION OF AN EXPRESSION VECTOR BASED ON pCDNA3.1(+) EXPRESSING JEMBRANA DISEASE VRIUS ENV-TM SUBUNIT GENE IN EUKARYOTIC SYSTEM Asmarani Kusumawati; Joannes Sri Widada; Basuki Hidayat
Jurnal Sain Veteriner Vol 22, No 1 (2004): Juli
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Faktor Resiko Bovine Brucellosis pada Tingkat Peternakan di Kabupaten Belu Propinsi Nusa Tenggara Timur Rosalinda Petra Maria Tae Lake; Asmarani Kusumawati; Setyawan Budiharta
Jurnal Sain Veteriner Vol 28, No 1 (2010): JUNI
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

KLONING DAN EKSPRESI GEN PENYANDI SAGI TOXOPLASMA GONDII ISOLAT LOKAL PADA VEKTOR pGEX-2T Sri Hartati; Hastari Wuryastuti; Joannes Sri Widada; Sumartono .; Asmarani Kusumawati
Jurnal Sain Veteriner Vol 22, No 2 (2004): DESEMBER
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

PENGARUH PEMBERIAN DEKSAMETASON PADA KELINCI LOKAL BUNTING YANG DIFETEKTOMI Slamet Soebagyo; Asmarani Kusumawati
Jurnal Sain Veteriner Vol 21, No 2 (2003): DESEMBER
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Co-Authors A. Junaidi Agung Budiyanto ANIS KARUNIAWATI Aris Junaidi B. Sardjono Bambang Sumiarto Bambang Sutrisno Basuki Hidayat Charles Rangga Tabbu Claude Mona Airin D. Tri Widayati Diah Tri Widayati Dini Wahyu Kartika Sari Dwi Priyowidodo Elly Widyarni Eka Purnamasari Hastari Wuryastuti HERA MAHESHWARI I Gusti Agung Komang Diafari Djuni Hartawan J.Sri Widada Joannes Sri Widada Joeannes Sri Widada Joko Prastowo K. Suharto Khairun Nissa Kresno Suharto Kurniasih . LUTHFIRALDA SJAHFIRDI Nafratilova Septiana Ni Wayan Kurniani Karja Penny Humaidah Hamid Pratiwi Pujilestari Sudarmono Pratiwi Sudharmono PUDJI ASTUTI Rarastoeti Pratiwi Rifia Tiara Fani Ronny Martien Rosalinda Petra Maria Tae Lake S. Sunendar Sarmin - Setyawan Budiharta Sitarina Widyarini Slamet Soebagyo Soesanto Mangkoewidjojo Sri Hartati Sri Suryatmiati Prihandani Sri Widada Sumartono . Sunendar - Surya Agus Prihatno Susan M Noor Susan M. Noor Susan Maphilindawati Noor Tenri Ashari Wanahari Tri Untari Yuli Purwandari Kristiangingrum

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