Article Per Year (5 Year)
p-Index From 2019 - 2024
1.203
P-Index
This Author published in this journals
All Journal Jurnal Teknologi Dan Industri Pangan Jurnal AGROTEKNOLOGI Agrointek Jurnal Pangan dan Agroindustri agriTECH TEKNOLOGI PANGAN : Media Informasi dan Komunikasi Ilmiah Teknologi Pertanian Advances in Food Science, Sustainable Agriculture and Agroindustrial Engineering (AFSSAAE) Berkala Penelitian Hayati
. Harijono
Fakultas Teknologi Pertanian, Universitas Brawijaya Malang, Jl. Veteran, Malang 65145
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Engineering Immunology & microbiology Industrial & Manufacturing Engineering Materials Science & Nanotechnology Mechanical Engineering Medicine & Pharmacology

Published : 23 Documents Claim Missing Document
Claim Missing Document
Check
Articles

Found 8 Documents
Search
Journal : agriTECH

 1 
Potensi Hipolipidemik Polisakarida Larut Air Umbi Gembili (Dioscorea esculenta L.) pada Tikus Hiperlipidemia Herlina Herlina; Harijono Harijono; Achmad Subagio; Teti Estiasih
agriTECH Vol 33, No 1 (2013)
Publisher : Faculty of Agricultural Technology, Universitas Gadjah Mada, Yogyakarta, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (218.072 KB) | DOI: 10.22146/agritech.9561

Abstract

This study aimed to determine the hypolipidemic effect of water soluble polysaccharides (WSP) of gembili tuber directly extracted with water (WSPc) and WSP, which was extracted with deproteinase process using protease enzymes from Aspergillus oryzae (WSPd). WSPc and WSPd were given to male wistar rats aged 2-3 month  were analyzed at total cholesterol, triglycerides, LDL cholesterol (Low Density Lipoprotein), HDL cholesterol (High Density Lipoprotein), digesta pH and SCFA (Short Chain Fatty Acid) of rat caecum digesta. The study was conducted for 4 weeks, rats were divided into 3 groups: control, WSPc and WSPd. Each group consisted of 6 rats, each group of rats was fed the same of the standard AIN-93M diet. WSPc and WSPd were given every day by forced feeding method at dose of 40 mg/100g weight of rats. A nested experimental design was employed in the experiment.  The results showed that the deproteinase process using Aspergillus oryzae protease 0.05% could reduce the protein levels of WSP gembili tuber 49.6%. PLAd was more potent hypolipidemic with WSPc and control. Giving WSPd during testing in rats can reduce the total cholesterol (52.92%), triglycerides (40.8%), LDL cholesterol (92,98%) and increased HDL cholesterol (46.95%). Fermentation of WSP in rats caecum produce SCFA  in the form of acetic acid, propionic acid and butyric acid.ABSTRAKPenelitian ini bertujuan untuk mengetahui efek hipolipidemik polisakarida larut air (PLA) umbi gembili yang diekstrak langsung dengan air (PLAc) dan PLA yang diekstrak dengan proses deproteinasi menggunakan enzim protease dari Aspergillus oryzae (PLAd). PLAc dan PLAd diberikan pada tikus wistar jantan umur 2-3 bulan untuk diuji kolesterol total, trigliserida, kolesterol LDL (Low Density Lipoprotein), kolesterol HDL (High Density Lipoprotein), pH digesta, dan SCFA digesta caecum tikus. Penelitian dilakukan selama 4 minggu pengujian, 18 ekor tikus dibagi menjadi 3 kelompok yaitu: perlakuan kontrol, PLAc dan PLAd. Setiap kelompok terdiri dari 6 ekor tikus, masing-masing kelompok tikus diberi ransum  yang sama yaitu diet standar AIN-93M. Pemberian PLAc dan PLAd dilakukan setiap hari selama pengujian dengan bantuan alat sonde (force feeding) dengan dosis 40 mg/100g berat tikus. Data yang diperoleh dianalisis dengan rancangan percobaan Rancangan tersarang (Nested Design). Hasil penelitian menunjukkan bahwa proses deproteinasi menggunakan protease Aspergillus oryzae 0,05% dapat mengurangi kadar protein PLA umbi gembili sebesar 49,6 %. PLAd lebih berpotensi sebagai hipolipidemik dibandingkan dengan PLAc dan kontrol. Pemberian PLAd umbi gembili selama pengujian pada tikus hiperlipidemia dapat menurunkan kolesterol total (52,92%), trigliserida (40,8%), kolesterol LDL (92,98%) dan peningkatan kolesterol HDL(46,95%). Hasil fermentasi PLA umbi gembili dalam caecum tikus menghasilkan SCFA (short chain fatty acid) berupa asam asetat, propionat dan butirat.
Potensi Hipolipidemik Polisakarida Larut Air Umbi Gembili (Dioscorea esculenta L.) pada Tikus Hiperlipidemia Herlina Herlina; Harijono Harijono; Achmad Subagio; Teti Estiasih
agriTECH Vol 33, No 1 (2013)
Publisher : Faculty of Agricultural Technology, Universitas Gadjah Mada, Yogyakarta, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (218.072 KB) | DOI: 10.22146/agritech.9561

Abstract

This study aimed to determine the hypolipidemic effect of water soluble polysaccharides (WSP) of gembili tuber directly extracted with water (WSPc) and WSP, which was extracted with deproteinase process using protease enzymes from Aspergillus oryzae (WSPd). WSPc and WSPd were given to male wistar rats aged 2-3 month  were analyzed at total cholesterol, triglycerides, LDL cholesterol (Low Density Lipoprotein), HDL cholesterol (High Density Lipoprotein), digesta pH and SCFA (Short Chain Fatty Acid) of rat caecum digesta. The study was conducted for 4 weeks, rats were divided into 3 groups: control, WSPc and WSPd. Each group consisted of 6 rats, each group of rats was fed the same of the standard AIN-93M diet. WSPc and WSPd were given every day by forced feeding method at dose of 40 mg/100g weight of rats. A nested experimental design was employed in the experiment.  The results showed that the deproteinase process using Aspergillus oryzae protease 0.05% could reduce the protein levels of WSP gembili tuber 49.6%. PLAd was more potent hypolipidemic with WSPc and control. Giving WSPd during testing in rats can reduce the total cholesterol (52.92%), triglycerides (40.8%), LDL cholesterol (92,98%) and increased HDL cholesterol (46.95%). Fermentation of WSP in rats caecum produce SCFA  in the form of acetic acid, propionic acid and butyric acid.ABSTRAKPenelitian ini bertujuan untuk mengetahui efek hipolipidemik polisakarida larut air (PLA) umbi gembili yang diekstrak langsung dengan air (PLAc) dan PLA yang diekstrak dengan proses deproteinasi menggunakan enzim protease dari Aspergillus oryzae (PLAd). PLAc dan PLAd diberikan pada tikus wistar jantan umur 2-3 bulan untuk diuji kolesterol total, trigliserida, kolesterol LDL (Low Density Lipoprotein), kolesterol HDL (High Density Lipoprotein), pH digesta, dan SCFA digesta caecum tikus. Penelitian dilakukan selama 4 minggu pengujian, 18 ekor tikus dibagi menjadi 3 kelompok yaitu: perlakuan kontrol, PLAc dan PLAd. Setiap kelompok terdiri dari 6 ekor tikus, masing-masing kelompok tikus diberi ransum  yang sama yaitu diet standar AIN-93M. Pemberian PLAc dan PLAd dilakukan setiap hari selama pengujian dengan bantuan alat sonde (force feeding) dengan dosis 40 mg/100g berat tikus. Data yang diperoleh dianalisis dengan rancangan percobaan Rancangan tersarang (Nested Design). Hasil penelitian menunjukkan bahwa proses deproteinasi menggunakan protease Aspergillus oryzae 0,05% dapat mengurangi kadar protein PLA umbi gembili sebesar 49,6 %. PLAd lebih berpotensi sebagai hipolipidemik dibandingkan dengan PLAc dan kontrol. Pemberian PLAd umbi gembili selama pengujian pada tikus hiperlipidemia dapat menurunkan kolesterol total (52,92%), trigliserida (40,8%), kolesterol LDL (92,98%) dan peningkatan kolesterol HDL(46,95%). Hasil fermentasi PLA umbi gembili dalam caecum tikus menghasilkan SCFA (short chain fatty acid) berupa asam asetat, propionat dan butirat.
Potensi Hipolipidemik Polisakarida Larut Air Umbi Gembili (Dioscorea esculenta L.) pada Tikus Hiperlipidemia Herlina Herlina; Harijono Harijono; Achmad Subagio; Teti Estiasih
agriTECH Vol 33, No 1 (2013)
Publisher : Faculty of Agricultural Technology, Universitas Gadjah Mada, Yogyakarta, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (218.072 KB) | DOI: 10.22146/agritech.9561

Abstract

This study aimed to determine the hypolipidemic effect of water soluble polysaccharides (WSP) of gembili tuber directly extracted with water (WSPc) and WSP, which was extracted with deproteinase process using protease enzymes from Aspergillus oryzae (WSPd). WSPc and WSPd were given to male wistar rats aged 2-3 month  were analyzed at total cholesterol, triglycerides, LDL cholesterol (Low Density Lipoprotein), HDL cholesterol (High Density Lipoprotein), digesta pH and SCFA (Short Chain Fatty Acid) of rat caecum digesta. The study was conducted for 4 weeks, rats were divided into 3 groups: control, WSPc and WSPd. Each group consisted of 6 rats, each group of rats was fed the same of the standard AIN-93M diet. WSPc and WSPd were given every day by forced feeding method at dose of 40 mg/100g weight of rats. A nested experimental design was employed in the experiment.  The results showed that the deproteinase process using Aspergillus oryzae protease 0.05% could reduce the protein levels of WSP gembili tuber 49.6%. PLAd was more potent hypolipidemic with WSPc and control. Giving WSPd during testing in rats can reduce the total cholesterol (52.92%), triglycerides (40.8%), LDL cholesterol (92,98%) and increased HDL cholesterol (46.95%). Fermentation of WSP in rats caecum produce SCFA  in the form of acetic acid, propionic acid and butyric acid.ABSTRAKPenelitian ini bertujuan untuk mengetahui efek hipolipidemik polisakarida larut air (PLA) umbi gembili yang diekstrak langsung dengan air (PLAc) dan PLA yang diekstrak dengan proses deproteinasi menggunakan enzim protease dari Aspergillus oryzae (PLAd). PLAc dan PLAd diberikan pada tikus wistar jantan umur 2-3 bulan untuk diuji kolesterol total, trigliserida, kolesterol LDL (Low Density Lipoprotein), kolesterol HDL (High Density Lipoprotein), pH digesta, dan SCFA digesta caecum tikus. Penelitian dilakukan selama 4 minggu pengujian, 18 ekor tikus dibagi menjadi 3 kelompok yaitu: perlakuan kontrol, PLAc dan PLAd. Setiap kelompok terdiri dari 6 ekor tikus, masing-masing kelompok tikus diberi ransum  yang sama yaitu diet standar AIN-93M. Pemberian PLAc dan PLAd dilakukan setiap hari selama pengujian dengan bantuan alat sonde (force feeding) dengan dosis 40 mg/100g berat tikus. Data yang diperoleh dianalisis dengan rancangan percobaan Rancangan tersarang (Nested Design). Hasil penelitian menunjukkan bahwa proses deproteinasi menggunakan protease Aspergillus oryzae 0,05% dapat mengurangi kadar protein PLA umbi gembili sebesar 49,6 %. PLAd lebih berpotensi sebagai hipolipidemik dibandingkan dengan PLAc dan kontrol. Pemberian PLAd umbi gembili selama pengujian pada tikus hiperlipidemia dapat menurunkan kolesterol total (52,92%), trigliserida (40,8%), kolesterol LDL (92,98%) dan peningkatan kolesterol HDL(46,95%). Hasil fermentasi PLA umbi gembili dalam caecum tikus menghasilkan SCFA (short chain fatty acid) berupa asam asetat, propionat dan butirat.
Potensi Hipolipidemik Polisakarida Larut Air Umbi Gembili (Dioscorea esculenta L.) pada Tikus Hiperlipidemia Herlina Herlina; Harijono Harijono; Achmad Subagio; Teti Estiasih
agriTECH Vol 33, No 1 (2013)
Publisher : Faculty of Agricultural Technology, Universitas Gadjah Mada, Yogyakarta, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (218.072 KB) | DOI: 10.22146/agritech.9561

Abstract

This study aimed to determine the hypolipidemic effect of water soluble polysaccharides (WSP) of gembili tuber directly extracted with water (WSPc) and WSP, which was extracted with deproteinase process using protease enzymes from Aspergillus oryzae (WSPd). WSPc and WSPd were given to male wistar rats aged 2-3 month  were analyzed at total cholesterol, triglycerides, LDL cholesterol (Low Density Lipoprotein), HDL cholesterol (High Density Lipoprotein), digesta pH and SCFA (Short Chain Fatty Acid) of rat caecum digesta. The study was conducted for 4 weeks, rats were divided into 3 groups: control, WSPc and WSPd. Each group consisted of 6 rats, each group of rats was fed the same of the standard AIN-93M diet. WSPc and WSPd were given every day by forced feeding method at dose of 40 mg/100g weight of rats. A nested experimental design was employed in the experiment.  The results showed that the deproteinase process using Aspergillus oryzae protease 0.05% could reduce the protein levels of WSP gembili tuber 49.6%. PLAd was more potent hypolipidemic with WSPc and control. Giving WSPd during testing in rats can reduce the total cholesterol (52.92%), triglycerides (40.8%), LDL cholesterol (92,98%) and increased HDL cholesterol (46.95%). Fermentation of WSP in rats caecum produce SCFA  in the form of acetic acid, propionic acid and butyric acid.ABSTRAKPenelitian ini bertujuan untuk mengetahui efek hipolipidemik polisakarida larut air (PLA) umbi gembili yang diekstrak langsung dengan air (PLAc) dan PLA yang diekstrak dengan proses deproteinasi menggunakan enzim protease dari Aspergillus oryzae (PLAd). PLAc dan PLAd diberikan pada tikus wistar jantan umur 2-3 bulan untuk diuji kolesterol total, trigliserida, kolesterol LDL (Low Density Lipoprotein), kolesterol HDL (High Density Lipoprotein), pH digesta, dan SCFA digesta caecum tikus. Penelitian dilakukan selama 4 minggu pengujian, 18 ekor tikus dibagi menjadi 3 kelompok yaitu: perlakuan kontrol, PLAc dan PLAd. Setiap kelompok terdiri dari 6 ekor tikus, masing-masing kelompok tikus diberi ransum  yang sama yaitu diet standar AIN-93M. Pemberian PLAc dan PLAd dilakukan setiap hari selama pengujian dengan bantuan alat sonde (force feeding) dengan dosis 40 mg/100g berat tikus. Data yang diperoleh dianalisis dengan rancangan percobaan Rancangan tersarang (Nested Design). Hasil penelitian menunjukkan bahwa proses deproteinasi menggunakan protease Aspergillus oryzae 0,05% dapat mengurangi kadar protein PLA umbi gembili sebesar 49,6 %. PLAd lebih berpotensi sebagai hipolipidemik dibandingkan dengan PLAc dan kontrol. Pemberian PLAd umbi gembili selama pengujian pada tikus hiperlipidemia dapat menurunkan kolesterol total (52,92%), trigliserida (40,8%), kolesterol LDL (92,98%) dan peningkatan kolesterol HDL(46,95%). Hasil fermentasi PLA umbi gembili dalam caecum tikus menghasilkan SCFA (short chain fatty acid) berupa asam asetat, propionat dan butirat.
Aktivitas Hidrolisis Enzim Lipase dari Kentos Kelapa terhadap Minyak Kelapa Mohammad Su’i; Harijono Harijono; Yunianta Yunianta; Aulani’am Aulani’am
agriTECH Vol 30, No 3 (2010)
Publisher : Faculty of Agricultural Technology, Universitas Gadjah Mada, Yogyakarta, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (407.029 KB) | DOI: 10.22146/agritech.9669

Abstract

This research was aimed to study hydrolysis conditions of houstorium lipases enzyme using coconut oil as substrate. Hydrolysis conditions studied were substrate (coconut oil) concentration, enzyme substrate ratio, duration of hydro- lysis and effect of stirring to hydrolysis. The results show  that lipase of coconut houstorium may be optimally used at a coconut oil concentration of 10 %, enzyme to substrate ratio of 3 : 10 (v/v) and hydrolysis for 60 minutes with stirring.ABSTRAKPenelitian ini mempelajari kondisi hidrolisis minyak kelapa yang optimum menggunakan enzim lipase dari kentos kelapa. Kondisi hidrolisis yang dipelajari meliputi konsentrasi substrat optium, perbandingan enzim : substrat dan lama hidrolisis yang optimum serta pengaruh pengadukan selama hidrolisis. Hasil penelitian menunjukkan bahwa, hidrolisis minyak kelapa menggunakan enzim lipase kentos kelapa menghasilkan asam lemak bebas paling banyak pada kon- sentrasi substrat (minyak kelapa) 10 %, perbandingan enzim : substrat yaitu 3 : 10 (v/v), lama hidroloisa 60 menit dan dilakukan pengadukan selama hidrolisis.
Aktivitas Hidrolisis Enzim Lipase dari Kentos Kelapa terhadap Minyak Kelapa Mohammad Su’i; Harijono Harijono; Yunianta Yunianta; Aulani’am Aulani’am
agriTECH Vol 30, No 3 (2010)
Publisher : Faculty of Agricultural Technology, Universitas Gadjah Mada, Yogyakarta, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (407.029 KB) | DOI: 10.22146/agritech.9669

Abstract

This research was aimed to study hydrolysis conditions of houstorium lipases enzyme using coconut oil as substrate. Hydrolysis conditions studied were substrate (coconut oil) concentration, enzyme substrate ratio, duration of hydro- lysis and effect of stirring to hydrolysis. The results show  that lipase of coconut houstorium may be optimally used at a coconut oil concentration of 10 %, enzyme to substrate ratio of 3 : 10 (v/v) and hydrolysis for 60 minutes with stirring.ABSTRAKPenelitian ini mempelajari kondisi hidrolisis minyak kelapa yang optimum menggunakan enzim lipase dari kentos kelapa. Kondisi hidrolisis yang dipelajari meliputi konsentrasi substrat optium, perbandingan enzim : substrat dan lama hidrolisis yang optimum serta pengaruh pengadukan selama hidrolisis. Hasil penelitian menunjukkan bahwa, hidrolisis minyak kelapa menggunakan enzim lipase kentos kelapa menghasilkan asam lemak bebas paling banyak pada kon- sentrasi substrat (minyak kelapa) 10 %, perbandingan enzim : substrat yaitu 3 : 10 (v/v), lama hidroloisa 60 menit dan dilakukan pengadukan selama hidrolisis.
Aktivitas Hidrolisis Enzim Lipase dari Kentos Kelapa terhadap Minyak Kelapa Mohammad Su’i; Harijono Harijono; Yunianta Yunianta; Aulani’am Aulani’am
agriTECH Vol 30, No 3 (2010)
Publisher : Faculty of Agricultural Technology, Universitas Gadjah Mada, Yogyakarta, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (407.029 KB) | DOI: 10.22146/agritech.9669

Abstract

This research was aimed to study hydrolysis conditions of houstorium lipases enzyme using coconut oil as substrate. Hydrolysis conditions studied were substrate (coconut oil) concentration, enzyme substrate ratio, duration of hydro- lysis and effect of stirring to hydrolysis. The results show  that lipase of coconut houstorium may be optimally used at a coconut oil concentration of 10 %, enzyme to substrate ratio of 3 : 10 (v/v) and hydrolysis for 60 minutes with stirring.ABSTRAKPenelitian ini mempelajari kondisi hidrolisis minyak kelapa yang optimum menggunakan enzim lipase dari kentos kelapa. Kondisi hidrolisis yang dipelajari meliputi konsentrasi substrat optium, perbandingan enzim : substrat dan lama hidrolisis yang optimum serta pengaruh pengadukan selama hidrolisis. Hasil penelitian menunjukkan bahwa, hidrolisis minyak kelapa menggunakan enzim lipase kentos kelapa menghasilkan asam lemak bebas paling banyak pada kon- sentrasi substrat (minyak kelapa) 10 %, perbandingan enzim : substrat yaitu 3 : 10 (v/v), lama hidroloisa 60 menit dan dilakukan pengadukan selama hidrolisis.
Aktivitas Hidrolisis Enzim Lipase dari Kentos Kelapa terhadap Minyak Kelapa Mohammad Su’i; Harijono Harijono; Yunianta Yunianta; Aulani’am Aulani’am
agriTECH Vol 30, No 3 (2010)
Publisher : Faculty of Agricultural Technology, Universitas Gadjah Mada, Yogyakarta, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (407.029 KB) | DOI: 10.22146/agritech.9669

Abstract

This research was aimed to study hydrolysis conditions of houstorium lipases enzyme using coconut oil as substrate. Hydrolysis conditions studied were substrate (coconut oil) concentration, enzyme substrate ratio, duration of hydro- lysis and effect of stirring to hydrolysis. The results show  that lipase of coconut houstorium may be optimally used at a coconut oil concentration of 10 %, enzyme to substrate ratio of 3 : 10 (v/v) and hydrolysis for 60 minutes with stirring.ABSTRAKPenelitian ini mempelajari kondisi hidrolisis minyak kelapa yang optimum menggunakan enzim lipase dari kentos kelapa. Kondisi hidrolisis yang dipelajari meliputi konsentrasi substrat optium, perbandingan enzim : substrat dan lama hidrolisis yang optimum serta pengaruh pengadukan selama hidrolisis. Hasil penelitian menunjukkan bahwa, hidrolisis minyak kelapa menggunakan enzim lipase kentos kelapa menghasilkan asam lemak bebas paling banyak pada kon- sentrasi substrat (minyak kelapa) 10 %, perbandingan enzim : substrat yaitu 3 : 10 (v/v), lama hidroloisa 60 menit dan dilakukan pengadukan selama hidrolisis.
Co-Authors AGUSTIN KRISNA WARDANI Aly Akbar Anna Ingani Widjajaseputra Aulani'am Aulia Azizah Syamsuri Chi-I Chang Desy Ambar Sari Dr.Ir. Yunianta, DEA Erni D.Sofia Murtini Feronika Heppy Sriherfyna Hera Sisca Prasmita, Hera Sisca I Komang Suwita Kgs Ahmadi KIKI FIBRIANTO Lulus Mualimin Moh. Sui Siti Narsito Wulan Sri Hastuti Suminar Diyah Nugraheni Syaiful Adnan Teti Estiasih Teti Estiasih Teti Estiasih Teti Estiasih Wenny Bekti Sunarharum