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All Journal Dentika Dental Journal Majalah Kedokteran Gigi Indonesia ODONTO Dental Journal Jurnal Perikanan Universitas Gadjah Mada Odonto dental journal
Heribertus Dedy Kusuma Yulianto
Departemen Biomedika, Fakultas Kedokteran Gigi, Universitas Gadjah Mada
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Dentistry Health Professions Immunology & microbiology Medicine & Pharmacology

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Journal : Majalah Kedokteran Gigi Indonesia

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Dealing with the high-risk potential of COVID-19 cross-infection in dental practice Yulianto, Heribertus Dedy Kusuma; Purwanti, Nunuk; Utami, Trianna Wahyu; Dewi, Anne Handrini; Listyarifah, Dyah; Ruspita, Intan; Nur, Asikin; Susilowati, Heni
Majalah Kedokteran Gigi Indonesia Vol 6, No 1 (2020): April
Publisher : Faculty of Dentistry, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/majkedgiind.56588

Abstract

The World Health Organization reported that the SARS-CoV-2 virus has infected more than 5 million people around the world. Dental care providers and health care professionals need to be aware of the high-risk potential of crossinfection since the routes of virus transmission commonly happen through droplets and aerosols. This review aimed at collecting essential knowledge about the COVID-19 needed by dental practitioners. The review focused on the oral involvement in COVID-19, the role of oral transmission as the high-risk potential of cross-infection and recommended strategies to minimize the risk of cross-infection in dental practice. We searched all the published clinical features from PubMed, Google Scholar, Scopus and hand searched library online databases, from January 2015 until May 2020. Keywords used were “COVID-19”, “Dentistry”, “Dental protection”, “Cross-contamination”, “Aerosol and non aerosol”, and ”Povidone-iodine” with their combinations. We identified 52 articles to review after the initial selection with inclusion and exclusion criteria. Results showed use of topical applications of povidine-iodine and viricidal mouthwash could significantly reduce the high-risk of cross-infection from dentistry patients who are asymptomatic with COVID-19 infection. Further safeguards include suspending all non-emergency procedures temporarily and closely screening patients for symptoms which may be suspected to be COVID-19 infection.
Dealing with the high-risk potential of COVID-19 cross-infection in dental practice Heribertus Dedy Kusuma Yulianto; Nunuk Purwanti; Trianna Wahyu Utami; Anne Handrini Dewi; Dyah Listyarifah; Intan Ruspita; Asikin Nur; Heni Susilowati
Majalah Kedokteran Gigi Indonesia Vol 6, No 1 (2020): April
Publisher : Faculty of Dentistry, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/majkedgiind.56588

Abstract

The World Health Organization reported that the SARS-CoV-2 virus has infected more than 5 million people around the world. Dental care providers and health care professionals need to be aware of the high-risk potential of crossinfection since the routes of virus transmission commonly happen through droplets and aerosols. This review aimed at collecting essential knowledge about the COVID-19 needed by dental practitioners. The review focused on the oral involvement in COVID-19, the role of oral transmission as the high-risk potential of cross-infection and recommended strategies to minimize the risk of cross-infection in dental practice. We searched all the published clinical features from PubMed, Google Scholar, Scopus and hand searched library online databases, from January 2015 until May 2020. Keywords used were “COVID-19”, “Dentistry”, “Dental protection”, “Cross-contamination”, “Aerosol and non aerosol”, and ”Povidone-iodine” with their combinations. We identified 52 articles to review after the initial selection with inclusion and exclusion criteria. Results showed use of topical applications of povidine-iodine and viricidal mouthwash could significantly reduce the high-risk of cross-infection from dentistry patients who are asymptomatic with COVID-19 infection. Further safeguards include suspending all non-emergency procedures temporarily and closely screening patients for symptoms which may be suspected to be COVID-19 infection.
The concentration effect of kulim leaf (scorodocarpus borneensis) extract on Streptococcus mutans ATCC 25175 bacterial hydrophobicity and adhesion Utami, Trianna Wahyu; Hapsari, Adhaninggar Ratna; Hanalda, Dhe Rifdania; Nur, Asikin; Yulianto, Heribertus Dedy Kusuma; Purwanti, Nunuk
Majalah Kedokteran Gigi Indonesia Vol 8, No 3 (2022): December
Publisher : Faculty of Dentistry, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/majkedgiind.80636

Abstract

With the ability to reduce hydrophobicity and inhibit the adhesion of S. mutans ATCC 25175 bacteria, Kulim leaf extract can be used as an alternative to chlorhexidine mouthwash in caries prevention. The objectives of this study are to determine the effect of Kulim leaf extract on hydrophobicity and adherence of the cariogenic bacteria S. mutans ATCC 25175. The test groups were divided into negative control (DMSO 2%), the treatment group (Kulim leaf extractconcentrations of 1.25%, 2.5%, 5%), and positive control (0.1% chlorhexidine). All of groups were received three replicated tests for hydrophobicity and adherence inhibition of S. mutans ATCC 25175 bacteria. The hydrophobicity test was conducted by providing 3 ml of bacterial suspension of S. mutans ATCC 25175 which had been adjusted to the McFarland 0.5 standard for each group. Furthermore, each test group was vortexed for one minute and left to stand for 15 minutes. Each treatment was tested with a wavelength spectrophotometer of 550 nm before and after the provision of 200 µl of n-hexadecane. The absorbance value on the spectrophotometer was then included in the hydrophobicity formula to determine the hydrophobicity percentage of S. mutans ATCC 25175 against n-hexadecane. To test the bacterial adhesion, the 96 wells microplate was inserted with the kulim leaf extract of each concentration,BHI-B, bacteria according to the McFarland 0.5 standard, and for the positive control and negative control. Afterwards, they were incubated at 37 °C for 24 hours before they were rinsed with distilled water, and stained with 0.1% crystal violet. Then, an optical density reading was performed using a microplate reader with a wavelength of 540 nm. The absorbance value was then included in the formula for percentage of bacterial adhesion inhibition. Post-Hoc LSD testshowed a significant difference in mean difference between the negative control group and the other treatment groups (p<0.05). In addition, it was revealed that there was no significant mean difference between treatment groups, and there was no significant difference between positive control and treatment groups of 2.5% and 5% in the hydrophobicity test. However, there was a significant difference between the positive control and the treatment group of 1.25% in the hydrophobicity test and the treatment group of 1.25%, 2.5%, and 5% in the adherence test. This study concluded that Kulim leaf extract concentration affected hydrophobicity and attachment of S. mutans ATCC 25175 with an effective concentration of 2.5%.
Co-Authors Anne Handrini Dewi Asikin Nur Deaoxi Renaschantika Djatumurti Dyah Listyarifah Dyah Listyarifah, Dyah Hanalda, Dhe Rifdania Hapsari, Adhaninggar Ratna Hendri Susanto Heni Susilowati Heni Susilowati Intan Ruspita Intan Ruspita, Intan Morita Nunuk Purwanti Nunuk Purwanti, Nunuk Puspita Hajardhini Roissatun Nasikah Trianna Wahyu Utami Zipora Silka Yoretina