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Kurniasih Kurniasih
Fakultas Kedokteran Hewan, Universitas Gadjah Mada, Yogyakarta
Veterinary

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Studi Lesi Makroskopis dan Mikroskopis Embrio Ayam yang Diinfeksi Virus Newcastle Disease Isolat Lapang yang Virulen Hamdu Hamjaya Putra; Haryadi Wibowo; Tri Untari; Kurniasih Kurniasih
Jurnal Sain Veteriner Vol 30, No 1 (2012): JUNI
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (7586.928 KB) | DOI: 10.22146/jsv.2468

Abstract

Newcastle disease (ND) is caused by Avian paramyxovirus, family Paramyxoviridae, one of the major diseases in chickens. This research was aimed to find lesions in chicken's embryo organs macroscopically andmicroscopically, infected by pathogenic ND virus. Embryonic chicken eggs (ECE) were inoculated by the ND Salatiga virus and ND La Sota virus as a control avirulent virus. Aquabidestilata used as a negative control. ECEwich showed the death of the embryos, stored in the refrigerator. The allantois fluid was collected, for further examination of viral growth. Chicken embryos that died then observed macroscopically. The organs of chicken embryos were made into histopathologic preparations stained with Hematoxylin and Eosin (H&E) for microscopic analysis. The identification of ND virus growth on isolates was done by haemagglutination andhaemagglutination inhibition test using an anti-ND serum. The chicken embryos that were infected by the ND Salatiga virus died approximately 26 hours post-inoculation. Macroscopic lesions were visible as haemorrhagein the skin. Microscopic lesions indicated the congestion and haemorrhage in lungs, inflammation and congestion in the skin, congestion in intestines, liver, kidneys and heart. There was also mild congestion on theskin in chicken embryos infected by ND La Sota virus. The microscopic lesions showed congestion in lungs, liver, kidneys and heart, also the inflammation and congestion on the skin. The macroscopic and microscopic lesions of chicken embryos infected by the ND Salatiga virus were more severe than lesions caused by ND La Sota virus.Key words: Newcastle disease, chicken embryos, macroscopic lesions, microscopic lesions, La Sota
Pengaruh Serbuk Jambe (Areca catechu) Dibandingkan Serbuk Gabungan Jambe (Areca catechu) dan Binahong (Anredera cordifolia) Terhadap Ascariasis pada Ayam Buras Okti Herawati; Kurniasih Kurniasih; Joko Prastowo
Jurnal Sain Veteriner Vol 36, No 1 (2018): Juni
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (8078.461 KB) | DOI: 10.22146/jsv.38449

Abstract

Infestasi Ascaridia galli mengakibatkan penurunan produktivitas dari ayam buras baik daging maupun telur. Pengendalian infestasi A. galli dilakukan menggunakan tanaman yang mengandung antiparasit sebagai anthelmintik. Penelitian ini bertujuan untuk mengetahui efek pemberian serbuk Jambe dan serbuk gabungan Jambe dengan Binahong terhadap infestasi Ascaridia galli pada ayam buras berdasarkan jumlah egg per gram (epg) feses, berat badan, jumlah cacing A.galli, dan perubahan histopatologi usus. Hewan yang digunakan dalam penelitian ini ialah ayam buras yang berumur 2 bulan dan positif terinfestasi cacing Ascaridia galli. Dilakukan penghitungan jumlah egg per gram (epg) dan berat badan sebelum perlakuan. Sebanyak 10 ekor ayam buras yang terbagi ke dalam 2 kelompok. Kelompok 1 diberi serbuk Jambe dengan dosis 0,1mg/kg BB sedangkan kelompok 2 diberi serbuk gabungan Jambe 0,05mg/kg BB dan Binahong 0,0125mg/kg BB. Pengobatan dilakukan secara per oral selama 7 hari, pada hari ke 10 dilakukan eutanasi. Data hasil perhitungan jumlah egg per gram (epg), jumlah cacing, berat badan dan perubahan histopatologi dianalisa secara diskriptif. Pemberian serbuk Jambe maupun serbuk gabungan Jambe dan Binahong dapat menurunkan jumlah epg, meningkatkan berat badan ayam dan membunuh cacing A.galli sebesar 80% untuk kelompok 1 dan 40% untuk kelompok 2. Perubahan histopatologis usus halus terlihat adanya infiltrasi sel radang berupa eosinofil dan limfosit di duodenum, jejunum dan ileum serta terdapat potongan cacing cestoda pada kedua kelompok. Serbuk Jambe lebih berpotensi sebagai anthelmentik dibandingkan serbuk gabungan Jambe dan Binahong dilihat dari penurunan jumlah epg, peningkatan berat badan dan jumlah cacing pada usus. Pemberian serbuk Jambe maupun serbuk gabungan Jambe dan Binahong dapat membunuh nematoda tetapi tidak untuk cestoda.
Immunodiagnosis Infeksi Aeromonas hydrophila pada Ikan Yuli Purwandari Kristianingrum; Sitarina Widyarini; Kurniasih Kurniasih; Bambang Sutrisno; Charles Rangga Tabbu; Sugiyono Sugiyono
Jurnal Sain Veteriner Vol 36, No 1 (2018): Juni
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (8456.913 KB) | DOI: 10.22146/jsv.38858

Abstract

Aeromonas hydrophila causes a disease that often infects fish and is known as Motile Aeromonas Septicaemia (MAS), Hemorrhagi Septisemia, Ulcer disease or Red-Sore disease. The   aims of this study were to develop polyclonal antibody of  Aeromonas hydrophila in the rabbits to   confirm the diagnosis of Aeromonas hydrophila  in the fish by immunohistochemistry staining method. Preparation of polyclonal antibodies was performed on the rabbits used to Aeromonas hydrophila bacteria that have been tested biochemically by intravenous and intraperitoneal injection. Doses of Aeromonas hydrophila  bacteria were 109 CPU/ml  of 0.5 ml at first injection, 1 ml at second injection, 2 ml at thirth injection and 3 ml at fourth injection. Blood serum collection was performed at week 5 after injection from  an  ear and intracardial vein. The result of antibody titer was 28 = 1024 which measured by   tube test. Furthermore, polyclonal   antibody was used to immunohistochemistry  staining with 400x dilution. The results of the staining showed that an immunopositive reaction in the liver, skin,lien,  gill, kidney, and heart of fish to Aeromonas hydrophila antibody. The research conclution was polyclonal antibody from rabbit can be used to accurately confirm the diagnosis of Aeromonas hydrophila  based on antigen and antibody reaction. 
Co-Authors Bambang Sutrisno Charles Rangga Tabbu Hamdu Hamjaya Putra Haryadi Wibowo Joko Prastowo Okti Herawati Sitarina Widyarini Sugiyono Sugiyono Tri Untari Yuli Purwandari Kristiangingrum