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All Journal Jurnal Sain Veteriner Jurnal Veteriner JURNAL PETERNAKAN Indonesian Journal of Biotechnology Jurnal Pengabdian Kepada Masyarakat (Indonesian Journal of Community Engagement)

Siti Isrina Oktavia Salasia

Department Of Clinical Pathology, Faculty Of Veterinary Medicine, Gadjah Mada University, Indonesia

Author-ID : 289882

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Decision Sciences, Operations Research & Management Immunology & microbiology Materials Science & Nanotechnology Veterinary

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PERANAN BUAH MENGKUDU (Morinda citrifolia) TERHADAP AKTIVITAS FAGOSITOSIS LEUKOSIT POLIMORFONUKLEAR TIKUS (Rattus noroegicus) YANG DIBERI DIET LEMAK TINGGI The Effect of Mengkudu (Morinda dtrifolia Linn.) to The Phagocytose Activity of Polymorphonuclear L Jemmi Setio Utomo; Rinto Sukoco; Adimas Galih; Siti Isrina Oktavia Salasia
Jurnal Peternakan Vol 3, No 2 (2006): September 2006
Publisher : State Islamic University of Sultan Syarif Kasim Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24014/jupet.v3i2.220

This study was done to evaluate the effect of mengkudu (Morinda citrifolia Linn.) to the phagocytose activity of polymorphonuclear leucocytes of high dietary fat of ram.Fifteen female Wistar albino ram (Rattus noroegicus) 1 month of age, were divided into 5 groups of 3 rats. The 1st group was fed with the basic food as a control. The 2N:1 group was fed with the basic food and 5% of fat The 3rd, 4111, and 5111 groups were fed with the basic food and 5% fat suplemented with mengkudu with the dose of 3,2 rot, 3,6 m1, and 4,0 ml/200 g body weight! oral/day for 2 months, respectively. At the end of the reasearch, the blood samples were collected from retroorbital plexus to evaluate the phagocytose activity of polymorphonuclear leucocytes in vitro. .The resulm of the reasearch were shown that the 5% diet fat of rats did not influence the phagocytose activity of polymorphonuclear leucocytes in vitro (22,13±1,33 bacteria/cell) compared to the control (21,11%3,59 bacteria/cell). Mengkudu with the dose of 3,6 ml/200 g body weight could increase the phagocytose activity of polymorphonuclear leucocytes (27,69%2,99 bacteria/cell) significantly (p<O,05) compared to the control (21,11%3,59 bacteria/cell) and the grouJ! ~hichfed the basic food and 5% of fat (22.13±1,33 bacterialcell).

Genetic Determination and Clonal Relationships of Staphylococcus aureus Isolated from Dairy Cows in Baturraden, Central Java, Indonesia Fatkhanuddin Aziz; Siti Isrina Oktavia Salasia; Mitra Slipranata
Indonesian Journal of Biotechnology Vol 19, No 2 (2014)
Publisher : Universitas Gadjah Mada

Cases of mastitis in cows at Baturraden are leading to signifi cant and ongoing problems due to reducedproduction and lower milk quality. This study was designed to identify which of selected virulence determinantgenes of S. aureus are involved in the Baturraden infection, and to determine the clonal relationship amongthese isolates. Seventeen isolates were identifi ed as S. aureus based on their biochemical properties and speciesspecifi city for 23S rRNA and nuc genes. S. aureus isolates were genotypically characterized for the selectedvirulence determinants: coa, clfA, fnbA, fnbB, cap5, spa IgG and spa X- region genes. Clonal relationship analysisamong isolates was carried out using AFLP and results compared with previously confi rmed relationshipsbetween selected S. aureus isolated from other regions. The results show that eight isolates contain all thegenes, but six isolates lack fnbB and two isolates lack cap5 genes. AFLP analysis showed that all isolates of S.aureus originating from cows in Baturraden belong to one cluster. This study provides additional knowledgeabout S. aureus infection in Baturraden cows, including the number of virulence determinant genes that mayplay a role in pathogenicity.

Determination of Haemaglutinin and Gene Encoding Fibronectin Binding Proteins Staphylococcus aureus Isolated from Dairy Milk Cows Feny Prabawati Pratomo; Siti Isrina Oktavia Salasia; Syarifudin Tato
Indonesian Journal of Biotechnology Vol 16, No 2 (2011)
Publisher : Universitas Gadjah Mada

Staphylococcus aureus is a major pathogen causing clinical and subclinical mastitis in dairy milk cows. Themastitis has immense economical impacts, where it reduces of the quantity and quality of milk production.The aims of the research were to analyse haemaglutinin and gene encoding fbronectin binding proteins.Nineteen Staphylococcus aureus isolates used in the present study were isolated from dairy milk cows fromYogyakarta, Solo, Boyolali and Sumedang. The haemagluitinin of S. aureus were determined based onhaemaglutination reaction to erythrocytes of rabbit. Detection of gene encoding fbronectin binding proteinscould be performed with specifc primers using polymerase chain reaction (PCR). The results of studiesshowed that most of S. aureus (78,95%) expressed haemaglutinin based on their ability to aglutinate rabbiterythrocytes. Analysis of gene encoding fbronectin binding proteins of S. aureus revealed gene fnbA withsize of approximately 1300 bp for 57,89% isolates, gene fnbB with size of approximately 900 bp for 31,58%isolates and both of gene fnbA and fnbB could be detected for 31,58% isolates. The characters of S. aureusbased on haemaglutinin, gene fnbA and fnbB of the present study could be used as an information to controlof S. aureus infection in dairy herds.

PROTOTIPE KTT DIAGNOSTIK UNTUK DETEKSI STREPTOKOKOSIS PADA PRIMATA DENGAN ELIS A-ANTIBODI MONOKLONAL PENANGKAP ANTIGEN Siti Isrina Oktavia Salasia; Aris Purwantoro; . Khusnan
Jurnal Sain Veteriner Vol 25, No 1 (2007): JUNI
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Penelitian ini bertujuan mengembangkan sarana diagnostik untuk kontrol streptococcosis pada primata dengan ELISA-monoclonal antibody penangkap antigen M-like protein (MLP) Streptococcus equi subsp. zooepidemiats grup C (SGC). M-like protein SGC diekstraksi dengan menggunakan lisozim dan Nasetilmuramidase. Protein dengan besar sekitar 58 kDa digunakan sebagai antigen untuk menimbulkan antibodi pada mencit Balb/c. Mencit yang mengandung antibodi dengan absorban tertinggi (2,868) diambil limpanya untuk memperolehl imfosit imUn( limfoblast). Hasil fusi sel mieloma dan limfoblast diperoleh4 klon hibridoma yang positif mengandung antibodi terhadap MLP, dengan nilai absorban pada ELISA masing-masing 1,900, 1,963, 1,895 dan 2,050. Hasil propagasi cairan asites mencit Balb/c diperoleh monoklonal antibodi terhadapMLP S. equi subsp. zooepidemicus dengan nilai absorban dan konsentrasi sebagai berikut: asitesI = 1,597(5,50mg), asites 2 : 1,940 (5,75 mg), dan asites 3 : 3,012 (5,80 mg). Antibodi monoklonal memperlihatkanspesifitas yang cukup tinggi karena hanya mengenal I epitop spesifik yang diperlihatkan pada uji Western blot dengan menampakkan pita tunggal pada sekitar 58 kDa dan menunjukkan reaksi positif pada uji dot-blot. Antibodi monoklonal memperlihatkan sensitifitas yang cukup tinggi setelah diuji dengan serum hewan percobaan tikus yang diinfeksi buatan dengan S. equi subsp. zooepidemicus dengan hasil absorban pada uji ELISA lebih dari 1,00 dan menunjukkan reaksi positif pada uji dot-blot. Hasil uji terhadap sampel serum Macacafascicularis menunjukkan bahwa 97 ,56% positif.Kata kunci: S. equi subsp. zooepidemicus, antibodi monoklonal, M-like protein, primata

RELATIONSHIP BETWEEN SURFACE HYDROPHOBICiTY, HAEMAGGLUTTNATION AND ADHERENCE MECHANISM OF STREPTOCOCCUS SUIS Siti Isrina Oktavia Salasia
Jurnal Sain Veteriner Vol 14, No 2 (1995)
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Streptococcus suis is well known as the causative agent of disease in various animal including pigs and human. The streptococcal cultures isolated from pigs and human have been characterized and identified biochemically and serologically as Streptococcus suis. The Cultures could be serotyped with 28 different monospecific antisera. Tne result of tins study indicated that the predominant antigen types of the S. suis cultures belonged to serotypes 2, 1/2, 1. The occurrence of the type specific microcapsule appeared to be realed with the surface hydrophobicity of the bacteria. Toe surface hydrophobicity could be demonstrated by hexadecan adherence tests. The S. suis cultures with hydrophobic surface agglutinated erythrocytes of humans, pigs and rabbits. The relationship between surface hydrophobicity, haemagglutinating and adherence properties of cultures could be demonstrated with 3 cell models. The haemagglutinating cultures adhered in high number to HeLa cells, ephitelial cells and to alveolar macrophages from rabbits. The non haemagglutinating cultures with hydrophobic surfaces adhered only to ephitelial cells and to alveolar macrophages S, suis cultures with hydrophilic surfaces showed no comparable reaction with 3 cell models.

Deteksi Gen Penyandi Sifat Resistensi Metisilin, Penisilin dan Tetrasiklin pada Isolat Staphylococcus aureus Asal Susu Mastitis Subklinis Sapi Perah Fatkhanuddin Aziz; Fajar Budi Lestari; Sarah Nuraidah; Endah Purwati; Siti Isrina Oktavia Salasia
Jurnal Sain Veteriner Vol 34, No 1 (2016): Juni
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Detection of gene encoding resistance of bacteria could be used as an accurate method to determine resistance of Staphylococcus aureus which is causing mastitis in dairy cows to the several antibiotics. This research aimed to detect the gene encoding resistance of methicillin, penicillin and tetracycline from identified S. aureus. Sixty milk samples were collected from subclinical mastitis of cows from various dairy farming in Yogyakarta. Isolation and identification of S. aureus based on the culture, Gram staining and biochemical test. Phenotypes of S. aureus resistances against antibiotics were carried out by disc diffusion method, meanwhilespecies specific gene of S. aureus and the gene encoding methicillin, penicillin and tetracycline were confirmed by PCR method. The results showed 11 isolates representing of Methicillin Susceptible Staphylococcus aureus (MSSA) could be identified, wherein 5 isolates were harboring both of penicillin and tetracycline resistant genes respectively.

Nano Spray TRISWHEAT (Teripang Super Wound Healing Agent) Penyembuh Luka Diabetes Mellitus yang Terinfeksi Bakteri MRSA (Methicillin Resistant Staphylococcus aureus) dengan Ekstrak Teripang Nada Hanifah; Yusuf Farid Achmad; Mellya Permatasari; Marista Kurniati; Ditya Tiwi Syafira; Siti Isrina Oktavia Salasia
Jurnal Sain Veteriner Vol 36, No 1 (2018): Juni
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Sea Cucumber (Stichopus sp) belongs to Holothuroidea family. Sea Cucumber has lot of uses in the medicine sector, such as: for wound healing, anti-biotic, anti-bacteria, anti-tumor, and anti-coagulation. Diabetes mellitus is a disease characterized by high blood sugar levels with metabolic disorders. This disease can cause wounds called gangrene This research has purpose to prove the influence of giving nano spray of sea cucumber extract within 14 days. In the in vitro examination found 40% concentration of sea cucumber extract which is effective to resist the growth of Methicillin Resistant Staphylococcus aureus bacteria and examined to wistar mouse which are already diabetic inducted with Streptozotocin (STZ) and hurt with punch biopsy with the diameter of 0,8 cm on its back and the wound is infected with bacteria which is intradermal injected. Divided into 3 groups : (K-) no medical treatment, nano spray of sea cucumber extract (T), Nano spray penicillin (K+) to wistar mouse towards the speed of diabetic mellitus wound healing which is infected by Methicillin Resistant Staphylococcus aureus bacteria. Parameter used to measure the rate of wound healing is wound size and hitopathology examination.After it’s regularly given the medicine, the result of nano spray sea cucumber extract 40 % concetration category treatment on wound healing process the diameter of wound on the 3rd day 0.8 cm , on the 7th day 0.4 cm and on the 14th day wound fully recover according to macros (wound size) and histopathology examination shows faster and better healing compared to treatment category of nano spray penicillin and control marked by score cell and lymphocyte in a small quantity.

Efek Antikariogenik Ekstrak Daun Beluntas (Pluchea indica) sebagai Penghambat Pertumbuhan Streptococcus Mutans penyebab Karies Gigi Nungki Fatimatuzzahra; Nourmalita Safitri Ningsih; Feny Feny; Adam Darsono; Siti Isrina Oktavia Salasia
Jurnal Sain Veteriner Vol 34, No 2 (2016): Desember
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Anti-plaque agents are known to reduce the formation of dental biofilms such as fluoride and chlorhexidine. However, excessive use of fluoride causes fluorosis and chlorhexidine side effects caused genotoxic. Pluchea indica L is one of the abundance of tropical herbs in the Asia-Pacific reported to have antiinflammatoryproperties and reduces the incidence of gastric necrosis, antinociception, neutralizing toxic venom, antioxidant, antiulcerative, hepatoprotective, antimoeba and broad-spectrum antimicrobial. This study examinesthe effectiveness anticariogenic Pluchea indica leaf extract in inhibiting the growth of Streptococcus mutans causing dental caries in vitro. Determination of Minimum Inhibitoric Concentration (MIC) on Pluchea indica leafextract is by diffusion test on the growth of Streptococcus mutans by Kirby Bauer Test. S. mutans concentration Mc Farland 0.5 standard (108 CFU ml-1) were inoculated in media Müller-Hinton agar (MHA). Paper diskcontaining Pluchea indica leaf extract at each concentration of 10%, 20%, 30%, 40%, 50%, 60%, and 70% were placed in an agar medium inoculated bacteria then incubated at 37°C on condition microaerophilia using candlejar. Determination of significance various Pluchea indica leaf extract concentration using one-way ANOVA test. The result of the diffusion method showed Pluchea indica leaf extract at a minimum concentration of 10% beenable to establish inhibited zone of Streptococcus mutans growth in MHA media. By one-way ANOVA test to various concentrations of extracts using Microsoft Excel 2007 have been obtained with F=4.85, Fcrit = 2.85 andP-value = 0.0071. Based on this research, it was concluded that Inhibitoric Minimum Concentration Pluchea indica leaf extract is capable of effective concentration of 10% and it has a significant (P-value< 0,05) of variousconcentration.

Potensi Ekstrak Daun Sage (Salvia officinalis.L) sebagai anti-Streptococcus suis Penyebab Zoonotik Meningitis Mitra Slipranata; Fajar Budi Lestari; Novra Arya Sandi; Siti Isrina Oktavia Salasia
Jurnal Sain Veteriner Vol 34, No 2 (2016): Desember
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

The phenomenon of microbial resistance to an antibiotic or some kind of specific antibiotics (multi drugs resistance) greatly complicate the treatment process, one of which is Streptococcus suis (S. suis) which is known to cause meningitis in animals and humans. Today, with rising bacterial resistance to a wide rangeof antibiotics, it takes an effort to assess the potential medicinal plants as an antibiotic that is appropriate and safe. Sage (Salvia officinalis.L) is reported to have antibacterial and fungicidal effect. Phenolic acids such assalvin and ether monomethyl salvin which isolated from the sage thought to have antimicrobial activity against several bacterial strains. The purpose of this study was to prove the ability of sage leaf extract as an antibacterialagainst S. suis causes streptococcal meningitis in vitro. In-vitro method used in this research through a two-stage dilution test and the diffusion test on Mueller Hinton Agar (MHA). S. suis isolates (code 225) were tested in vitro against 8 levels sage leaf extract concentration, ie a concentration of 1%, 3% 5%, 7%, 10%, 20%, 40% and 60%. The results of the test obtained by minimum inhibitory concentrations (MICs) and minimum sage known to potentially inhibit the growth of S. suis.

Potensi Ekstrak Atuna racemosa sebagai Anti - Methicillin Resistant Staphylococcus aureus (MRSA) Siti Isrina Oktavia Salasia; Novra Arya Sandi; Fajar Budi Lestari; Verda Farida; Nurbani Aziz
Jurnal Sain Veteriner Vol 35, No 2 (2017): Desember
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Staphylococcus aureus is one of the major causative agents of mastitis in animals and a variety of human diseases such as septicemia, endocarditis, arthritis dan osteomyelitis. Infection of Methicillin-resistant S. aureus (MRSA) has been widely reported and these strains are usually resistant to multiple antibiotics. The purpose of this study was to evaluate the potential of Atuna racemosa, as an alternative herbal medicine against MRSA infection. The MRSA strains were isolated from human and confirmed based on their resistant to various antibiotics and analyzing of the mecA gene by polymerase chain reaction (PCR). Atuna racemosa originated from Ambon, Maluku, Indonesia, were extracted using 70% ethanol. The activities of the Atuna racemosa extract against MRSA were performed by diffusion disc agar and dilussion agar tests. The results showed that Atuna racemosa extract has the barrier effect of MRSA growth at a concentration of 5% in the diffusion test and at a concentration of 7% in the dilution test. Atuna racemosa could be used as an alternative new drugs with dose of 0.07 g/ml (7%) against MRSA which is multi-resistant to many antibiotics.

Co-Authors Achmad Fauzi Adam Darsono Adimas Galih Aris Purwantoro Ditya Tiwi Syafira Endah Purwati Fajar Budi Lestari Fatkhanuddin Aziz Fatkhanuddin Aziz, Fatkhanuddin Fauziah Fitriana Feny Feny Feny Prabawati Pratomo Jemmi Setio Utomo Khusnan k Madarina Wasissa Marista Kurniati Mellya Permatasari Mitra Slipranata Mitra Slipranata Muhammad Novrizal Abdi Sahid Nada Hanifah Nourmalita Safitri Ningsih Novra Arya Sandi Novra Arya Sandi Nungki Fatimatuzzahra Nurbani Aziz Nurulia Hidayah Rinto Sukoco Riza Resita Sarah Nuraidah Sarasati Windria Syarifudin Tato Widianingrum, Desy Cahya Yusuf Farid Achmad

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