Article Per Year (5 Year)
p-Index From 2019 - 2024
0.562
P-Index
This Author published in this journals
All Journal Jurnal Sain Veteriner Jurnal Veteriner JURNAL PETERNAKAN Indonesian Journal of Biotechnology Jurnal Pengabdian Kepada Masyarakat (Indonesian Journal of Community Engagement)
Siti Isrina Oktavia Salasia
Department Of Clinical Pathology, Faculty Of Veterinary Medicine, Gadjah Mada University, Indonesia
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Decision Sciences, Operations Research & Management Immunology & microbiology Materials Science & Nanotechnology Veterinary

Published : 12 Documents Claim Missing Document
Claim Missing Document
Check
Articles

Found 12 Documents
Search

 1   2 
The Empowerment of Livestock Farmers in Subclinical Mastitis Test with GAMA Anti-Haptoglobin in “Sahabat Ternak” Etawah Crossbreed Goat Farm Fajar Budi Lestari; Madarina Wasissa; Muhammad Novrizal Abdi Sahid; Siti Isrina Oktavia Salasia
Jurnal Pengabdian kepada Masyarakat (Indonesian Journal of Community Engagement) Vol 8, No 1 (2022)
Publisher : Direktorat Pengabdian kepada Masyarakat Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (530.747 KB) | DOI: 10.22146/jpkm.60490

Abstract

Sahabat Ternak is one of the Etawah crossbreed (PE) goat farm groups in Sleman. This farm group focuses on goat milk production and processed goods. Problems that commonly arise in dairy goat farms are the cases of subclinical mastitis, which are quite high. This disease may cause a decrease in milk production and quality. The mastitis subclinical detection method which is often used by the farming community is the somatic cell count (SCC) and California mastitis test (CMT). However, both tests have low accuracy. Recently, a new method named GAMA Anti-Haptoglobin, which is more accurate and can be done easily has been established by livestock farmers. This community service aims to empower livestock farmers in applying GAMA Anti-Haptoglobin as a sensitive, rapid, and accurate subclinical mastitis detection kit in Sahabat Ternak goat farm. The method used in this activity consisted of discussion, socialization, and training for livestock farmers, as well as laboratory testing, evaluation of test findings, and treatment for PE goats. After the training, the livestock farmers were able to apply GAMA Anti-Haptoglobin mastitis detection method effectively. The implementation of this easy and accurate field mastitis detection method, as well as personnel with reliable skills, will support in the decrease of mastitis cases and increase milk production and quality, as well as community welfare.
Deteksi dan Analisis Filogenetik Staphylococcal Enterotoxin Y Isolat Bakteri Staphylococcus aureus Asal Kambing Peranakan Etawah Fatkhanuddin Aziz; Desy Cahya Widianingrum; Sarasati Windria; Siti Isrina Oktavia Salasia; Nurulia Hidayah; Achmad Fauzi; Fauziah Fitriana; Riza Resita
Jurnal Veteriner Vol 23 No 4 (2022)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19087/jveteriner.2022.23.4.458

Abstract

Staphylococcus aureus diketahui sering menyebabkan radang ambing pada ternak perah dan memproduksi berbagai jenis enterotoksin yang berpotensi sebagai agen penyebab keracunan makanan melalui susu maupun produk olahan susu yang dikonsumsi. Staphylococcal enterotoxin Y (SEY) asal penyakit kulit pada manusia dilaporkan tahan terhadap uji pemanasan dan menyebabkan muntah pada hewan coba primata yang menunjukkan potensinya sebagai salah satu penyebab keracunan makanan. Pada penelitian ini, deteksi gen sey pada 18 isolat S. aureus asal kambing Peranakan Etawah (PE) dengan teknik PCR, kemudian dilakukan sekuensing gen sey dari isolat S. aureus MR6. Sekuens basa nukleotida ditranslasi menjadi protein menggunakan EMBOSS Transeq (https://www.ebi.ac.uk/Tools/st/emboss_transeq/). Sekuens SEY MR6 selanjutnya dibandingkan dengan database genebank SEY dan enterotoksin lain yang berasal dari isolat S. aureus asal manusia dan sapi menggunakan MultAlin (http://multalin.toulouse.inra.fr/multalin/cgi-bin/multalin.pl). Pohon filogenetik dari protein SEY yang dibandingkan, dibuat menggunakan MEGA Software 5.0. Hasil PCR diketahui 28% isolat, positif gen sey. Analisis SEYcap dari isolat S. aureus kambing PE diketahui mempunyai persentase protein sekuens homologi sebesar 97,3% terhadap SEY asal isolat sapi perah dan manusia. Diketahui terdapat 6 posisi residu asam amino yang berbeda antar SEY yang diperbandingkan. Analisis filogenetik menunjukkan kedekatan kekerabatan dengan SEY asal manusia, namun masih 1 kluster dengan SEY asal sapi.
Co-Authors Achmad Fauzi Adam Darsono Adimas Galih Aris Purwantoro Ditya Tiwi Syafira Endah Purwati Fajar Budi Lestari Fatkhanuddin Aziz Fatkhanuddin Aziz, Fatkhanuddin Fauziah Fitriana Feny Feny Feny Prabawati Pratomo Jemmi Setio Utomo Khusnan k Madarina Wasissa Marista Kurniati Mellya Permatasari Mitra Slipranata Mitra Slipranata Muhammad Novrizal Abdi Sahid Nada Hanifah Nourmalita Safitri Ningsih Novra Arya Sandi Novra Arya Sandi Nungki Fatimatuzzahra Nurbani Aziz Nurulia Hidayah Rinto Sukoco Riza Resita Sarah Nuraidah Sarasati Windria Syarifudin Tato Widianingrum, Desy Cahya Yusuf Farid Achmad