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All Journal Agrotrop : Journal on Agriculture Science International Journal of Biosciences and Biotechnology Journal of Agricultural Science and Biotechnology Bumi Lestari Ecotrophic, Journal of Environmental Science E-Jurnal Agroekoteknologi Tropika (Journal of Tropical Agroecotechnology) Nandur
Khamdan Khalimi
Program Studi Agroekoteknologi Fakultas Pertanian Universitas Udayana
Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Earth & Planetary Sciences Energy Environmental Science

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UJI EFEKTIVITAS RIZOBAKTERI SEBAGAIA GEN ANATGONIS Sclerotium rolfsii PENYEBAB PENYAKIT BUSUK BATANG PADA TANAMAN KACANG TANAH (Arachis hypogea L.) Ni Kadek Desy Andya Dewi; Dewa Ngurah Suprapta; Khamdan Khalimi
Journal of Agricultural Science and Biotechnology Vol 8 No 1 (2019)
Publisher : Journal of Agricultural Science and Biotechnology

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Abstract

Groundnut (Arachis hypogea L.) is one of important commodities in Indonesia, however the production of groundnut is still relatively low which ispartly because of the plant disease i.e. stem rot disease caused by Sclerotium rolfsii. Rhizobacteria is one of the solutions to be used as the biological controlagent against S. rolfsii. The aim of this study is to know the effectiveness of rhizobacteria to control stem rot disease caused by S. rolfsii. The results the studyshowed that among 130 isolates of rhizobacteria tested against S. rolfsii, 11 isolates showed inhibitory activity, and among them one isolate, BjTk11 fromexposed the highest inhibitory activity (96.09%) against S. rolfsii. On a green house experiment, treatment with formula of isolate BjTk 11 at doses of 5 g, 10 g,15 g and 20 g per polybag effectively controlled the pre emergence damping off (disease incidence = 0%) while the disease incidence on control was 23.5%. Nopost emergence damping off symptom was observed on groundnut treatment with formula of isolate BjTk11, while the disease incidence on control was 75%. The number of sclerotia in soil of control was 79/gram of soil which is obviously higher than those of treatments. The mode of action the rhizobacteria isolatesBjTk11 in inhibiting the growth of S. rolfsii is through antibiosis where the rhizobacteria produce the antifungal compound Butanedial, R - (-) - 1,2propanediol, 2,3-Butanediol, Isopropyl Alcohol, Acetic acid, anhydride with formic Acid , 2-bromohexane, 4,5-Diamino-6-hydroxypyrimidine, 4H-Pyran-4one, 3-hydroxy-2-methyl and Cyclotrisiloxane, hexamethyl-. The presence of these compounds probably closely related to the inhibitory activity against S. rolfsii.
Pemanfaatan Rizobakteri Penghasil Indole Acetic Acid Sebagai Agens Penginduksi Ketahanan Tanaman Tembakau Terhadap Cucumber Mosaic Virus (CMV) Ni Luh Gede Sriyanti; Khamdan Khalimi; I Gede Rai Maya Temaja
Journal of Agricultural Science and Biotechnology Vol 8 No 1 (2019)
Publisher : Journal of Agricultural Science and Biotechnology

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Abstract

Tobacco is a high economic value commodity, in order to meet the demand for tobacco in the market it requires good quantity and quality. One of the diseasethat often causes harm to tobacco is Cucumber Mosaic Virus (CMV). The utilization of indole acetic acid (IAA)-producing rhizobacteria can be used to increase plant growth and resistance from pathogenic infection. The aim of this study was to determine the effectiveness of IAA producing rhizobacteria formula in increasing the growth of tobacco plants in greenhouses, and to determine the effectiveness of rhizobacterial formulations in increasing tobacco plant resistanceagainst the infection of CMV. Identification of compounds in tobacco leaves that infected with CMV was carried out to determine the effectiveness of rhizobacteria in inducing plant resistance. Compound analysis was carried out using Gas Mass Chromatography Spectroscopy (GCMS). The effectiveness of rhizobacteria in increasing plant growth and resistance was conducted in a greenhouse. Based on the results of the study, four of IAA-producing rhizobacteria isolates were Stenotrophomonas maltophilia Sg3, Proteus mirabilis BjB17, Bacillus thuringiensis TNJbx3.3, and Bacillus cereus GR12, all the rhizobacterial isolates were effective in increasing plant height, leaf number, leaf chlorophyll content, wet weight and dry weight leaf. Based on the results of the rhizobacterial effectiveness test, all rhizobacterial isolates were effective in increasing plant resistance as indicated by a lower intensity of infection. Based on the analysis of the tobacco leaves compounds content showed that rhizobacteria S. maltophilia Sg3 and P. mirabilis BjB17 were able to induce resistance to tobacco plants by forming antiviral compounds namely 2-Naphthalene-sulfonic acid and rhizobacteria Bacillus cereus GR12 can stimulate the formation of 1-Naphthalene-sulfonic acid compounds and 2-Naphthalenesulfonic acidin tobacco as a form of induction of resistance to CMV infection.
POTENSI RIZOBAKTERI YANG DIISOLASI DARI RIZOSFER TANAMAN PADI SEBAGAI AGEN HAYATI UNTUK MENGHAMBAT PERTUMBUHAN JAMUR Pyricularia oryzae, PENYEBAB PENYAKIT BLAS PADA TANAMAN PADI Valente Quintao; Dewa Ngurah Suprapta; I Gede Rai Maya Temaja; Khamdan Khalimi
Journal of Agricultural Science and Biotechnology Vol 4 No 1 (2015)
Publisher : Journal of Agricultural Science and Biotechnology

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Abstract

Rice (Oryza sativa L.) is one of the staple foods for more than 60 percent of world population. Indonesian people generally are still depending on the availability of rice to fulfill Indonesian food demand. The average of rice productivity in Indonesia is 4.56 ton/ha which is relatively lower when compared with other rice growing countries, such as Australia 8.22 ton/ha;  Japan 5.85 ton/ha and China 6.06 ton/ha. One of the causes for the low productivity of rice in Indonesia is the occurrences of rice blast disease caused by Pyricularia oryzae.  To control this disease, the farmers are still rely on the use of synthetic fungicides however this measure can not effectively control the disease,  and potentially cause the health and environmental problems. It is necessary to find an alternative measure  that save to human health as well as friendly to the environment. This study was  done to evaluate the potential use of rhizobacteria isolated from rhizospheres of rice as antagonist against Pyricularia oryzae the  cause of rice blast disease.  The result showed that five isolates of rhizobacteria namely  Xanthomonas lumininescens isolate Ch3Da, Serratia liquefaciens isolate Gh13DaB, Enterobacter agglomerans isolate Gg14DtB, Enterobacter agglomerans isolate Ch2Da, and Enterobacter agglomerans isolate Ch4BaB significantly (P<0.05) inhibited the growth of Pyricularia oryzae on potato dextrose agar (PDA) medium with inhibitory activity varied from 39.46% to 46.66%. All of these isolates produced extra cellular substances which probably responsible for the inhibitory activity. This result suggested that five isolates of rhizobacteria tested in this study can be further developed as bio-control agent to reduce the development of rice blast disease.
UJI ANTAGONIS BAKTERI ENDOFIT TERHADAP Colletotrichum scovillei PENYEBAB PENYAKIT ANTRAKSONA PADA CABAI BESAR (Capsicum annuum L.) I Gede Karta Satria Wibawa; Dewa Ngurah Suprapta; Khamdan Khalimi
Journal of Agricultural Science and Biotechnology Vol 8 No 1 (2019)
Publisher : Journal of Agricultural Science and Biotechnology

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Abstract

Colletotrichum scovillei is a newly discovered species causing anthracnose disease on chilli pepper (Capsicum annuum L.) in Bali. With the increasing reports of Colletotricum spp. resistance against chemical fungicide, biological control is taken as the alternatives to control this disease. In this study a total of 69 endophytic bacteria isolates was recovered from different solanaceae plants samples collected from nine regions of Bali and screened for the antagonism againstC. scovillei. From the potential 15 candidates of isolates with inhibition activity above 90%, hypersensitivity test were taken on tobacco leaf, and isolate CBCKB1 was chosen for further test. The CBCKB1 isolate was identified as Bacillus amyloliquefaciens based on molecular identification. Detached fruit bioassay proved that isolate CBCKB1 formula was effective in reducing the disease percentage and disease severity of anthracnose disease on chilli pepper.
POTENSI RIZOBAKTERI YANG DIISOLASI DARI RIZOSFIR TANAMAN GRAMINAE NON-PADI UNTUK MEMACU PERTUMBUHAN BIBIT PADI Ni Made Intan Maulina; Khamdan Khalimi; Gusti Ngurah Alit Susanta Wirya; Dewa Ngurah Suprapta
Journal of Agricultural Science and Biotechnology Vol 4 No 1 (2015)
Publisher : Journal of Agricultural Science and Biotechnology

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Abstract

Rice (Oriza sativa L.) is the main food for Indonesian people, however the rice production is not enough to fulfill all national demand. Rice productivity in Indonesia was about 4,5 ton/ha, relatively low when compared to other rice growing countries such Australia, Japan, and China. It is necessary to find a measure to increase the rice productivity in Indonesia. One of the Measure is the use of rhizobacteria isolated from the rhizozpheres of the plants belongs to the family Graminae. This study was done in order to find potential rhizobacteria from rhizospheres of five plant species belong to the family Graminae, namely Cymbopogon ciatrus, Imperata cylindrica, Saccharum officinarum, Pennisetum purpureum and Schizostachum mosum. Result of this study showed that 10 isolates of rhizobacteria obviously increased the number of lateral roots of rice seedling cultivar Ciherang. The rice seedling treated with isolate Al7Kla produced the highest number of lateral roots when compared to other isolates. This isolate may be used to promote the rice growth in order to increase the yield.
PEMANFAATAN RAGI (SACCAROMYCES SP.) DALAM PENGENDALIAN PENYAKIT TUMBUHAN YANG RAMAH LINGKUNGAN Khamdan Khalimi
Bumi Lestari Journal of Environment Vol 10 No 2 (2010)
Publisher : Environmental Research Center (PPLH) of Udayana University

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Abstract

One of the most important challenges is preventing and controlling pest and diseases problems, which can partly or completely ruin agricultural crops. Survey done in Bali at three main vanilla growing areas indicated that the stem rot disease was the main and the most destructive disease on vanilla. The objective of the research is to control the vanilla stem rot disease caused by pathogenic fungus Fusarium oxysporum f, sp.vanillae. The test for antagonistic activity by fungi was done through side by side culture and antifungal activity test on vanilla seedlings. Results of this study showed that Saccaromyces sp.effectively suppressed the growth of fungi on Potato Dextrose Agar medium and vanilla seedlings.
PEMANFAATAN PLANT GROWTH PROMOTING RHIZOBACTERIA UNTUK BIOSTIMULANTS DAN BIOPROTECTANTS Khamdan Khalimi; Gusti Ngurah Alit Susanta Wirya
ECOTROPHIC : Jurnal Ilmu Lingkungan (Journal of Environmental Science) Vol 4 No 2
Publisher : Master Program of Environmental Science, Postgraduate Program of Udayana University

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Abstract

Various findings on the benefit of plant growth promoting rhizobacteria (PGPR) for agriculture have been reported by many research institutional. The enthusiasm to commercialize these bacteria as a promising alternative technology is triggered mainly by the to develop environmentally benign agriculture by reducing the use of synthetically agrochemical inputs (fertilizers and pesticides). These result suggested that application of PGPR could promoted the plant growth and increase the resistance of plant against fungi pathogen.
Uji Daya Hambat Bakteri Paenibacillus polymyxa terhadap Pertumbuhan Jamur Colletotrichum sp. Secara In Vitro IDA AYU ISTRI MAYADIANTI; KHAMDAN KHALIMI; NI WAYAN SUNITI
Jurnal Agroekoteknologi Tropika (Journal of Tropical Agroecotechnology) Vol.9, No.4, Oktober 2020
Publisher : Program Studi Agroekoteknologi, Fakultas Pertanian, Universitas Udayana

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Abstract

Inhibition Test of Paenibacillus polymyxa on the Growth of the Fungus Colletotrichum sp. in vitro Fungus Colletotrichum sp. is an airborne pathogen that causes anthracnose disease in chili. Utilization of biological agents of Paenibacillus polymyxa is the choice in suppressing the growth and fungal attack of Colletotrichum sp. The use of biological agency is environmentally friendly. The purpose of this study was to determine the inhibitory properties of P. polymxya bacteria against the growth of the fungus Colletotrichum sp. in vitro.The results showed that P. polymyxa isolates C1 was able to inhibit the growth of C. acutatum isolates BLCB, C. gloesporioides isolates KLCR2, C. scovillei isolates SGCR, C. fructicola isolates KRCR, C. truncatum isolates DPCR2 and C. nymphae isolates BLCR, with the percentage of inhibition ranging from 82.74% to 86.52%. Bacterial filtrate P. polymyxa C1 was able to inhibit the growth of fungal colonies of C. acutatum isolate BLCB, C. gloesporioides isolate KLCR2, C. scovillei isolates SGCR , C. fructicola isolates KRCR, C. truncatum isolates DPCR2 and C. nymphaeae isolates BLCR with inhibitory power percentage ranging from 71.90% to 82.48%.
Uji Aktivitas Antijamur Bacillus cereus terhadap Colletotrichum fructicola KRCR Penyebab Penyakit Antraknosa pada Buah Cabai Rawit (Capsicum frutescens L.) KRISNA SANUBARI PURBA; KHAMDAN KHALIMI; NI WAYAN SUNITI
Jurnal Agroekoteknologi Tropika (Journal of Tropical Agroecotechnology) Vol.10, No.1, Januari 2021
Publisher : Program Studi Agroekoteknologi, Fakultas Pertanian, Universitas Udayana

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Abstract

Test of Antifungal Activity of Bacillus cereus Against Colletotrichum fructicola Causing Anthracnose Disease in Cayenne Pepper (Capsicum frutescent L.) Anthracnose or fruit rot disease that attacks ceyenne pepper is caused by the pathogenic fungus Colletotrichum fructicola, this disease can cause crop failure. Currently, control of this disease is still using synthetic fungicides, but this method can cause demage to the ecosystem. Biopesticides are one of the enviromentally friendly control of plant phatogens because biopesticides use biologycal agents. The purpose of this study was to determine the ability of B. cereus bacteria to inhibit the growth of C. fructicola KRCR cause anthracnose desease in vitro. The result showed that bacteria B. cereus was able to inhibit the growth of fungal colonies C. fructicola KRCR on potato dextrose agar (PDA) with an inhibitory percentage of 90.55% when compared to controls. The results of the B. cereus filtrate test a concentration of 50% is able to inhibit the growth of C. fructicola KRCR with an inhibitory percentage of 87.56%.
Pengujian Bakteri Endofit Indigenous dalam Mengendalikan Penyebab Penyakit Busuk Buah Kakao (Phytophthora palmivora Butler) Secara In Vitro SAMI MAIDA SIJABAT; I MADE SUDARMA; KHAMDAN KHALIMI
Jurnal Agroekoteknologi Tropika (Journal of Tropical Agroecotechnology) Vol.10, No.4, Oktober 2021
Publisher : Program Studi Agroekoteknologi, Fakultas Pertanian, Universitas Udayana

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Abstract

Testing Indigenous Endophytic Bacteria in Controlling Causes of Cacao Rot Disease (Phytophthora palmivora Butler) In Vitro Phytophthora palmivora is the cause of important diseases in cacao, including pod rot. Fruit rot is the most dominant disease because it causes great losses. The use of biological agents is an environmentally friendly way to treat rot disease. One of these biological agents is endophytic bacteria in inhibiting the growth of the fungus P. palmivora which causes pod rot disease in cocoa (Theobroma cacao L.). The research was conducted in vitro. The results of the isolation and inhibition test showed that 1 isolat of endophytic bacteria had the best inhibition, namely BC2 isolat which was able to control P. palmivora fungi disease with an inhibitory percentage of 95.47% when compared to the control at 5 days after inoculation. The results of the inhibition test of the bacterial filtrate of BC2 isolat against P. palmivora at a concentration of 50% showed that the bacterial filtrate of BC2 isolates was able to inhibit the growth of P. palmivora fungi with an inhibitory percentage of 99.80% when compared to the control. The results of the inhibition test of BC2 isolat bacteria on P. palmivora fungi biomass showed that BC2 isolat bacteria were able to inhibit the formation of fungi biomass by 72.72% when compared to the control.
Co-Authors ANINDA OKTAVIA RAHARINI Dewa Ngurah Suprapta DONI SITUMORANG Gede Wijana GUSTI AYU KOMANG CANDRA PARWATI Gusti Ngurah Alit Susanta Wirya HENRI PAN BAGUS MULIA NAPITUPULU I Dewa Nyoman Nyana I G. R. M. TEMAJA I GEDE KARTA SATRIA WIBAWA I Gede Karta Satria Wibawa I Gusti Made Wira Kesuma Jaya I GUSTI NGURAH RAKA I Ketut Siadi I MADE SUDARMA I MADE SUDARMA I NYOMAN GEDE ASTAWA I PUTU WIRYA SUPUTRA IDA AYU ISTRI MAYADIANTI KEZIA KEZIA KOMANG ADI MAHARTHA KRISNA SANUBARI PURBA Ni Kadek Desy Andya Dewi NI KOMANG SRI BAWANTARI Ni Luh Gede Sriyanti Ni Luh Putu Citra Innosensia NI MADE INDRA PUSPAWATI Ni Made Intan Maulina NI WAYAN SUNITI NURI MANDAN SARI PARDIANTA PATRISIUS SINAGA Putu Perdana Kusuma Wiguna Retno Kawuri ROYAN PRACAHYO SAMI MAIDA SIJABAT SOLICHATUN SOLICHATUN SUSAN CAMILA FOEH TRISNA AGUNG PHABIOLA Valente Quintao WAYAN ADIARTAYASA