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All Journal Agrotrop : Journal on Agriculture Science journal of internal medicine AGRITROP Journal of Agricultural Science and Biotechnology Jurnal Veteriner Bumi Lestari Ecotrophic, Journal of Environmental Science E-Jurnal Agroekoteknologi Tropika (Journal of Tropical Agroecotechnology) Biogenesis: Jurnal Ilmiah Biologi Jurnal Ilmiah Teknologi Pertanian Agrotechno MNJ (Malang Neurology Journal) JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA Agrica: Journal of Sustainable Dryland Agriculture Indonesian Journal of Pharmacology and Therapy
Dewa Ngurah Suprapta
Program Studi Agroekoteknologi Fakultas Pertanian Universitas Udayana
Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Chemistry Computer Science & IT Earth & Planetary Sciences Energy Environmental Science Immunology & microbiology Medicine & Pharmacology Neuroscience Veterinary

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EFEK SITOTOKSIK DAN ANTIPROLIFERATIF EKSTRAK ETANOL UMBI UBI JALAR UNGU (Ipomoea batatas L) TERHADAP SEL LINE KANKER PAYUDARA T47D 1 Sumardika, I Wayan; Wiwiek Indrayan, Agung; Jawi, I Made; Suprapta, Dewa Ngurah; Adnyan, Losen
journal of internal medicine Vol. 11, No. 1 Januari 2010
Publisher : journal of internal medicine

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Abstract

Cancer is still one of the leading death causes worldwide. Consuming antioxidants can reduce the cancer incidence. Anthocyanin that contained in purple sweet potatoes can inhibit DNA damages. In order to prove the activity of purple sweet potatoes on cancer cell, it is necessary to have some direct research on cancer cell line.This study is a simple experimental research method. The activities of the anticancer are evaluated from cytotoxic and antiproliferative effects on cell line breast cancer T47D. Cytotoxic effects is examined with cultured cell stained with tryphan blue exlusion. Each well are contain of 36,000 cells, which is given ethanol extract purple sweet potatoes with 10 dose variation starting from 500  g/mL up to 10,000  g/mL replicated three times, then the inhibitory percentage is calculated. Antiproliferative activity is evaluated by incubating cancer cells that have been given ethanol extract purple sweet potatoes in 3 doses variation; 500, 1,000 and 2,000  g/ml in 24, 48 and 72 hours.Result of this study showed that ethanol extract purple sweet potatoes had cytotoxic activities on T47D breast cancer cell line in these dose variations, 500; 1,000; 2,000; 3,000; 4,000; 5,000; 6,000; 7,000; 8,000; 9,000 and 10,000  g/ml. The cytotoxic activity on each of dose variations above are 27.56; 42.67; 57.78; 66.67; 72.44; 79.56; 85.33; 87.56; 92.44;100; and 100 %. Ethanol extract purple sweet potatoes showed antiproliferative activities on T47D breast cancer cells in 24, 48, 72 hours incubation in concentrations 500; 1,000; and 2,000  g/ml; those are 35,700; 29,800; 25,500 cells (24 hours); 72,500; 60,300; 52,600 cells  (48 hours); 149,500;122,600;107,300 cells  (72 hours). In conclusion, ethanol extract purple sweet potatoes had cytotoxic and antiproliferative activities on cell line breast cancer T47D.
PENGENDALIAN PENYAKIT BUSUK BUAH KAKAO DENGAN PESTISIDA NABATI SUDARMA, I MADE; SUPRAPTA, D. N.
AGRITROP Vol. 28, No. 1 Maret 2009
Publisher : AGRITROP

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Abstract

Pod rot disease in Cacao (Theobroma cacao) caused by Phytophthora palmivora has been known to cause serious damage at cacao-growing area in the world. The disease can cause crop loss until 90%. Hitherto cacao farmer controls disease only on usage of synthetic pesticide. Therefore important is performed a operation research of disease with other alternative, like usage of leaf extract Piper betle and clove flowers (Eugenia caryophillata), as botanical pesticide. Research has been conducted in Pulukan village, Sub-Province Jembrana in January until April 2006, to study botanical pesticide effectivity (plant extract) to control pod rot disease. Research applies completely randomized block design, with four treatments and six replications, causing is required 24 experiment units. Every re-attempt unit thrice and ten tree pears observed as sample. Disease percentage is observed every one week once in each treatment. Results of the experiment indicated that leaf extract of P. betle and clove flowers effective controls pod rot disease (alone or in combination). The extract mixture P. betle and clove flower (combination) can suppress disease until 64,57 %, clove flowers extract (alone) 33,33% and synthetic pesticide (Dithane M45) equal to 60,40%. Result of this depicts treatment tested is usable to reduce pathogen pod rot disease of cacao.
UPAYA MEMINIMALISIR DAMPAK LINGKUNGAN DARI PENGGUNAAN PESTISIDA DALAM PERTANIAN (DAMPAK LINGKUNGAN DAN PENANGGULANGANNYA) Jayanti , Hadis; Suprapta , Dewa Ngurah
AGRICA Vol. 2 No. 1 (2009): June
Publisher : Agriculture Faculty of Flores University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37478/agr.v2i1.541

Abstract

Utilization of synthetic pesticide seems up to now remains to be one of the methods in controlling pest and plant disease because it plays a role in determining the quality and quantity of the crops. Improver of synthetic pesticide in agriculture can adversely affect the ecosystem such as water and soil pollution. The occurrence of pest resistance, the outbreak of secondary pest, the resurgence of pest, the death of predators, the residue of pesticide in food, and the pesticide residue that affects man's health. Although it has been widely known that pesticide has a bad impact on the environment, the use of pesticide is still needed in agricultural practice. Its use needs to pay attention to the concept of Integrated Pest Management in which the use of pesticide is only made when it is absolutely necessary, that is, when the population of pest increases and lies above one level of the pest population, the Economic Threshold (Untung, 1993). To know the safety level of pesticide, evaluation of toxicity of a pesticide is needed and it is commonly tested in the animal of high level. This is done in such a way that the toxicity and safety level of the pesticide can be known by farmers, users of pesticide, and the environment. Based on the information on the toxicity level, it is expected that the steps of environmental management can be taken to minimize the negative impact of pesticide. The effort of minimizing the negative impact of pesticide can be done in a variety of ways. For example, the direction was given to the users in terms of giving the dosage, application time, safe working procedure, choosing pesticide with the precise target, choosing a pesticide that can decompose easily, application target, a time before harvest, tool and exact application technique, keeping as small pollution as possible. In the future, it is hoped that the use of pesticide will decrease and will be more selective, and supported by new inventions in the technology of processing pesticide disposal and the technology of utilizing botanical pesticide which is all expected to be effective in overcoming the disturbance of pest and plant disease at the same time minimizing environmental degradation.
POTENTIAL OF ANTHOCYANIN BASED POLY (METHYL METHACRYLATE) NANOPARTICLES SPECIFIC ACTIVATED MICROGLIA IN MANAGEMENT INFLAMMATORY PAIN ON HERNIATED NUCLEUS PULPOSUS: A LITERATURE REVIEW Widyadharma, I Putu Eka; Satyarsa, Agung Bagus Sista; Sanjaya, Feliani; Gitari, Ni Made; Niryana, I Wayan; Purwata, Thomas Eko; Jawi, I Made; Suprapta, Dewa Ngurah; Sudewi, AA Raka
Malang Neurology Journal Vol 7, No 1 (2021): January
Publisher : Malang Neurology Journal

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.mnj.2021.007.01.9

Abstract

Pain is an unpleasant sensory and emotional experience that can affect the quality of life and leads to decreased productivity in patients. Low back pain (LBP) is one of the significant causes of disability worldwide with lifelong incidence. The purpose of this literature review describes the potential of anthocyanin-based Poly (Methyl Methacrylate) (PMMA)   nanoparticles as the management of inflammatory pain in the Hernia Nucleus Pulposus (HNP ). The method used is a literature study by entering the keyword. Of the 77 journals reviewed, 47 journals were found by the topic and used as a reference for this work. The anthocyanin-based PMMA nanoparticles act as anti-nociceptors by inhibiting microglia that produce inflammatory mediators in HNP. Poly (Methyl Methacrylate) nanoparticles have specific targets in microglia. Anthocyanins have the effect of inhibiting inflammatory pain through many destinations. Anthocyanin inhibits the synthesis of nitric oxide (NO ) and prostaglandin E2 (PGE 2) and inhibits the activation of p38 MAPK and NF-kB pathways that express TNF-α and IL-1β genes as anti-nociceptive. The anthocyanin-based PMMA nanoparticles have potential as a novel therapy for inflammatory pain in HNP. There has been no research between these modalities. Therefore, further research is needed to find out the exact potential of anthocyanin-based PMMA nanoparticles.
UTILIZATION OF BETEL LEAVES EXTRACT TO PREVENT THE GROWTH OF Fusarium oxysporum f.sp. capsici 1 CAUSING FUSARIUM WILT DISEASE IN BELL PEPPER Leana, Ni Wayan Anik; Suprapta, Dewa Ngurah
JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA Vol. 21 No. 1 (2021): MARCH, JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA
Publisher : Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jhptt.12126-33

Abstract

Utilization of betel leaves extract to prevent the growth of Fusarium oxysporum f.sp. capsici causing fusarium wilt diseasein bell pepper. Betel (Piper betle Linn.) is commonly used in Indonesian traditional medicine due to its antimicrobial properties,thus it is often used as an ingredient in natural pesticides. The effectiveness of betel leaf extract as a control for Fusariumoxysporum in several plants showed it’s potential to control the fungi in bell pepper. F. oxysporum f.sp. capsici is one of theimportant pathogens causing Fusarium wilt disease in bell pepper. It is one of the most devastating plant diseases due to itsability to cause a crop failure. The test on the inhibitory capacity of betel leaf extract on the growth of F. oxysporum f.sp.capsici were carried out by growing the fungi on PDA mixed with various concentrations of betel leaf extracts. The resultsshowed that betel leaf extract treatment at 0.02% concentration was able to inhibit the growth of F. oxysporum f.sp. capsici inPDA at 95.54%. The full inhibition of colony growth (100%) was achieved in the extract treated with the concentration of0.03%. Meanwhile treatment of 0.17% betel leaf extract was able to inhibit the growth of F. oxysporum f.sp. capsici on bellpepper stems. Following this result, fractionation of the betel leaf extract by column chromatography was conducted, resultingin 44 fractions. The bioassays of those fractions showed that, there were seven fractions that reveal inhibition capabilityagainst F. oxysporum f.sp. capsici.
Isolation and molecular identification of fungi causing stem rot disease in Bali's local legumes Ni Made Susun Parwanayoni; Dewa Ngurah Suprapta; Nyoman Darsini; Sang Ketut Sudirga
Biogenesis: Jurnal Ilmiah Biologi Vol 9 No 1 (2021)
Publisher : Department of Biology, Faculty of Sci and Tech, Universitas Islam Negeri Alauddin Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24252/bio.v9i1.20426

Abstract

Efforts to improve food security in Indonesia, particularly Bali, need to be supported by improvements in cultivation techniques, including the management of pests and diseases. Meanwhile, legume crops are often attacked by stem rot diseases which potentially decrease production and leads to economic losses. This disease is generally caused by the soil-borne pathogenic fungus Sclerotium rolfsii or Athelia rolfsii. The macroscopic and microscopic morphologies of these two species are the same and difficult to distinguish, hence, molecular identification is needed to differentiate between the species. Therefore, this study aims to isolate and molecularly identify the fungi causing stem rot disease in local legume plants in Bali. The methods used include isolation of pathogenic fungi from legumes showing symptoms of stem rot disease, pathogenicity test, identification of isolates with the highest virulent levels, DNA extraction, DNA amplification by PCR and electrophoresis, ITS region sequencing and computer analysis sequences. The isolation procedure identified six fungal isolates coded SKT, SKB1, SKB2 SKB3, SKL and SKN isolates. SKT isolates had the highest virulence rate as indicated by the pathogenicity test of peanut plants. Furthermore, molecular identification results show that SKT isolate is Athelia rolfsii, a similar clade with the fungi sequences in GenBank with 100% bootstrap support.
UJI EFEKTIVITAS RIZOBAKTERI SEBAGAIA GEN ANATGONIS Sclerotium rolfsii PENYEBAB PENYAKIT BUSUK BATANG PADA TANAMAN KACANG TANAH (Arachis hypogea L.) Ni Kadek Desy Andya Dewi; Dewa Ngurah Suprapta; Khamdan Khalimi
Journal of Agricultural Science and Biotechnology Vol 8 No 1 (2019)
Publisher : Journal of Agricultural Science and Biotechnology

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Abstract

Groundnut (Arachis hypogea L.) is one of important commodities in Indonesia, however the production of groundnut is still relatively low which ispartly because of the plant disease i.e. stem rot disease caused by Sclerotium rolfsii. Rhizobacteria is one of the solutions to be used as the biological controlagent against S. rolfsii. The aim of this study is to know the effectiveness of rhizobacteria to control stem rot disease caused by S. rolfsii. The results the studyshowed that among 130 isolates of rhizobacteria tested against S. rolfsii, 11 isolates showed inhibitory activity, and among them one isolate, BjTk11 fromexposed the highest inhibitory activity (96.09%) against S. rolfsii. On a green house experiment, treatment with formula of isolate BjTk 11 at doses of 5 g, 10 g,15 g and 20 g per polybag effectively controlled the pre emergence damping off (disease incidence = 0%) while the disease incidence on control was 23.5%. Nopost emergence damping off symptom was observed on groundnut treatment with formula of isolate BjTk11, while the disease incidence on control was 75%. The number of sclerotia in soil of control was 79/gram of soil which is obviously higher than those of treatments. The mode of action the rhizobacteria isolatesBjTk11 in inhibiting the growth of S. rolfsii is through antibiosis where the rhizobacteria produce the antifungal compound Butanedial, R - (-) - 1,2propanediol, 2,3-Butanediol, Isopropyl Alcohol, Acetic acid, anhydride with formic Acid , 2-bromohexane, 4,5-Diamino-6-hydroxypyrimidine, 4H-Pyran-4one, 3-hydroxy-2-methyl and Cyclotrisiloxane, hexamethyl-. The presence of these compounds probably closely related to the inhibitory activity against S. rolfsii.
POTENSI RIZOBAKTERI YANG DIISOLASI DARI RIZOSFER TANAMAN LEGUMINOSAE UNTUK MENGENDALIKAN JAMUR Sclerotium rolfsii PENYEBAB PENYAKIT REBAH KECAMBAH PADA TANAMAN KEDELAI Komang Adi Mahartha; Dewa Ngurah Suprapta; Gusti Ngurah Alit Susanta Wirya
Journal of Agricultural Science and Biotechnology Vol 6 No 1 (2017)
Publisher : Journal of Agricultural Science and Biotechnology

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Abstract

Damping off disease caused by Sclerotium rolfsii is an important disease of soybean, because soybean plant will die after attacked by pathogen. This pathogen can survive for a long time in the soil by sclerotia form. Some of rhizobacteria isolated from the rhizospheres of Leguminosae plants were known could control soil-borne pathogens. This study aims to test rhizobacteria isolated from the rhizosphere of Leguminosae plants that able to suppress the growth of S. rolfsii. Total of 11 isolates of 112 isolates rhizobacteria that could be isolated from Leguminosae plants had antifungal activity against S. rolfsii. Inhibition test of 11 isolates of rhizobacteria against S. rolfsii showed that three isolates had the highest ability to inhibit the growth of S. rolfsii namely isolates KtD1, KtD6, and KtB3. This result suggested that three isolates of rhizobacteria tested in this study can be further developed as bio-control agent to reduce the development of damping off disease on soybean.
POTENSI RIZOBAKTERI YANG DIISOLASI DARI RIZOSFER TANAMAN PADI SEBAGAI AGEN HAYATI UNTUK MENGHAMBAT PERTUMBUHAN JAMUR Pyricularia oryzae, PENYEBAB PENYAKIT BLAS PADA TANAMAN PADI Valente Quintao; Dewa Ngurah Suprapta; I Gede Rai Maya Temaja; Khamdan Khalimi
Journal of Agricultural Science and Biotechnology Vol 4 No 1 (2015)
Publisher : Journal of Agricultural Science and Biotechnology

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Abstract

Rice (Oryza sativa L.) is one of the staple foods for more than 60 percent of world population. Indonesian people generally are still depending on the availability of rice to fulfill Indonesian food demand. The average of rice productivity in Indonesia is 4.56 ton/ha which is relatively lower when compared with other rice growing countries, such as Australia 8.22 ton/ha;  Japan 5.85 ton/ha and China 6.06 ton/ha. One of the causes for the low productivity of rice in Indonesia is the occurrences of rice blast disease caused by Pyricularia oryzae.  To control this disease, the farmers are still rely on the use of synthetic fungicides however this measure can not effectively control the disease,  and potentially cause the health and environmental problems. It is necessary to find an alternative measure  that save to human health as well as friendly to the environment. This study was  done to evaluate the potential use of rhizobacteria isolated from rhizospheres of rice as antagonist against Pyricularia oryzae the  cause of rice blast disease.  The result showed that five isolates of rhizobacteria namely  Xanthomonas lumininescens isolate Ch3Da, Serratia liquefaciens isolate Gh13DaB, Enterobacter agglomerans isolate Gg14DtB, Enterobacter agglomerans isolate Ch2Da, and Enterobacter agglomerans isolate Ch4BaB significantly (P<0.05) inhibited the growth of Pyricularia oryzae on potato dextrose agar (PDA) medium with inhibitory activity varied from 39.46% to 46.66%. All of these isolates produced extra cellular substances which probably responsible for the inhibitory activity. This result suggested that five isolates of rhizobacteria tested in this study can be further developed as bio-control agent to reduce the development of rice blast disease.
ISOLASI DAN IDENTIFIKASI JAMUR PENGHASIL MIKOTOKSIN PADA BIJI KAKAO KERING YANG DIHASILKAN DI FLORES Emilia Simpllisiu Ake Wangge; Dewa Ngurah Suprapta; Gusti Ngurah Alit Susanta Wirya
Journal of Agricultural Science and Biotechnology Volume 1, No 1, Tahun 2012
Publisher : Journal of Agricultural Science and Biotechnology

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Abstract

A study entitled “Isolation and Identification of Mycotoxin-producing Fungi on Cocoa Beans Produced in Flores” was done in order to know the population and species of fungi and to know the concentration of mycotoxin in the dried cocoa beans. The dry cocoa beans used in this study were collected from several locations in Flores. Isolation of the fungi was done using Sabouraud Dextrosa Agar (SDA), while the determination of mycotoxin concentration was done based on Enzym Linked Immunosorbent Assay (ELISA). Results of this study showed that,  there were several fungi were found in the dried cocoa beans produced in Flores, namely Aspergillus niger, A. flavus, A. parasiticus, A. fumigatusandMucor sp. All tested dried cocoa beans contained aflatoxin B1, where the highest aflatoxin B1 concentration was found in the fermented beans from West Manggarai with the concentration at 3.65 ppb, while the lowest concentration was 2.21ppb, which was found in the cocoa beans from Nagekeo. The highest concentration of ochratoxin A was 0.38  ppb, which was found in the cocoa beans from West Manggarai, while no ochratoxin A was detected in the cocoa beans from Ende.
Co-Authors Agung Bagus S. Satyarsa Agung Wiwiek Indrayan Agus Selamet Duniaji ALPIN JUAN HAGATA PINEM Anak Agung Ngurah Subawa BERTA BR GINTING Emilia Simpllisiu Ake Wangge Gitari, Ni Made Gusti Ngurah Alit Susanta Wirya Hadis Jayanti HENRI PAN BAGUS MULIA NAPITUPULU I B Yoga I Dewa Made Sukrama I G. R. M. TEMAJA I Gede Karta Satria Wibawa I GEDE KARTA SATRIA WIBAWA I Ketut Suada I MADE ADNYANA I Made Jawi I Made Sudana I MADE SUDARMA I Putu Eka Widyadharma I WAYAN GENTA ARIAWAN I Wayan Niryana I Wayan Sumardika Jayanti , Hadis K Agung Sudewa Ketut Suata Khamdan Khalimi Komang Adi Mahartha l MADE SUDARMA LASTRI APRIANI Leana, Ni Wayan Anik Losen Adnyan M. Sudiana Mahendra Made Agus Hendrayana MADE SUSUN PARWANAYONI Meitini Proborini Wahyuni Ni Kadek Desy Andya Dewi NI KOMANG SRI BAWANTARI NI LUH GEDE SRIYANTI NI LUH PUTU SONIA SAVITA DEWI Ni Made Intan Maulina Ni Nyoman Puspawati Ni Putu Ristiati NI WAYAN SUNITI NOVA SULIYANTI SIANTURI Nyoman Darsini PARDIANTA PATRISIUS SINAGA Raka-Sudewi A. A. SANG KETUT SUDIRGA Sanjaya, Feliani Sukmawati Sukmawati Sukmawati Sukmawati Thomas Eko Purwata Valente Quintao