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All Journal HAYATI Journal of Biosciences Journal of Tropical Biodiversity and Biotechnology Darmabakti Cendekia: Journal of Community Service and Engagements Berkala Penelitian Hayati
Sugiharto
Department Of Biology, Faculty Of Science And Technology, Universitas Airlangga, Mulyorejo, Surabaya, East Java 60115; Biotechnology Of Tropical Medicinal Plants Research Group, Faculty Of Science And Technology, Universitas Airlangga, Mulyorejo, Sur
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Computer Science & IT Earth & Planetary Sciences Economics, Econometrics & Finance Engineering Environmental Science Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Public Health Social Sciences

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Gynura procumbens Adventitious Root Extract Altered Expression of Antioxidant Genes and Exert Hepatoprotective Effects Against Cadmium-Induced Oxidative Stress in Mice Sugiharto; Dwi Winarni; Anjar Tri Wibowo; Ufairanisa Islamatasya; Idqa Nurtri Bhakti; Nabilatun Nisa; Boon Chin Tan; Yosephine Sri Wulan Manuhara
HAYATI Journal of Biosciences Vol. 29 No. 4 (2022): July 2022
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.29.4.479-486

Abstract

Exposure to cadmium (Cd) could increase of reactive oxygen species (ROS) and changes in expression of antioxidant genes. Gynura procumbens is a medicinal plant that is rich in phenolic and flavonoid compounds. The aimed of study to evaluate the hepatoprotective effect of G. procumbens adventitious root (GAR) extract against Cd toxicity, especially expression rate of hepatic antioxidant genes. Twenty-five male mice were treated as follows: P1 (control), P2 (Cd100mg/L), P3 (GAR100mg/L + Cd100mg/L), P4 (GAR200mg/L + Cd100mg/L), and P5 (GAR300mg/L + Cd100mg/L). The samples (blood and liver) were collected for analysis of malondialdehyde (MDA) levels, superoxide dismutase (SOD) and catalase (CAT) activities, and their relative gene expression were determined. The hematological assay showed Cd-treated administered with GAR extract increased the number of red blood cells (RBC), haemoglobin concentration (HGB), hematocrit (HCT), mean corpuscular volume (MCV), and mean corpuscular haemoglobin (MCH), but reduced the level of alanine aminotransferase (ALT) and aspartate aminotransferase (AST). In addition, the GAR extract decreased the MDA production, but increased the activities of SOD and CAT. These enzymatic activities were positively correlated with their respective gene transcripts. Our study revealed that GAR extract administration showed marked hepatoprotective effects against Cd-induced oxidative stress.
EMPOWERMENT OF YOUTH COMMUNITY IN POSO REGENCY, THROUGH PRODUCE TOMATO AND MELON BY HYDROPONIC SYSTEM Yosephine Sri Wulan Manuhara; Alfinda Novi Kristanti; Sugiharto Sugiharto; R. Djarot Sugiarso; Yohanes Kartjito Putro; Arga Wal Yudha
Darmabakti Cendekia: Journal of Community Service and Engagements Vol. 4 No. 1 (2022): JUNE 2022
Publisher : Faculty of Vocational Studies, Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (602.059 KB) | DOI: 10.20473/dc.V4.I1.2022.8-15

Abstract

Background: Tentena City is one of the tourist centers with rapid economic growth located in Poso Regency because Poso Lake is very famous and able to change the economic level of the community. But there are still 20% of people who live very poor and 60% of the lower middle class and the remaining 20% of the middle to upper middle. Through the youth community Pukat Tentena is expected to foster and encourage millennials to develop vegetable and fruit entrepreneurs using more modern cultivation techniques. Objectives: empowering the youth community in Tentena city, Pamona Puselemba Subdistrict, Poso Regency, Central Sulawesi with entrepreneurial tomato fruit products and melons produced by hyrdoponik drip irrigation system. Methods: This community service activity was carried out in Tentena village, Pamona Puselemba Subdistrict, Poso Regency, Central Sulawesi Province with TENTENA PUKAT Youth Community partners starting from May to September 2021. Results: Mitra has successfully cultivated tomato and melon plants with hydroponic drip irrigation system techniques in green houses until they are obtained salesable results. The average number of tomatoes per stalk is 4.05 and the average fruit diameter is 5.1 cm; While the average weight of melons is 1.2 -1.8 kg. Conclusion: The results of this community service activity provide benefits for partners, namely providing additional knowledge about how to cultivate tomatoes and melons with hydroponic drip irrigation system techniques and increase partner income by selling tomatoes and melons from hydroponic cultivation. 
EFEKTIVITAS KURKUMIN SEBAGAI ANTIOKSIDAN DAN INHIBITOR MELANIN PADA KULTUR SEL B16-F1 Sugiharto; Arbakariya Ariff; Syahida Ahmad; Muhajir Hamid
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 17 No 2 (2012): June 2012
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (231.789 KB) | DOI: 10.23869/211

Abstract

Melanin inhibitors have become increasingly important ingredients in medication and cosmetics for the prevention of hyperpigmentation. In the last few years, a huge number of natural herbal extracts have been tested as inhibitors of melanin synthesis and some of these effects are related to the antioxidant properties. The objectives of this study were to determine of curcumin properties as antioxidant activity and melanin inhibitors. In this study, our data indicated that antioxidant assay with DPPH showed IC50 was 16,05 μg/ml. In the absence of α-MSH (α-Melanocyte Stimulating Hormone), melanin content assay in cell B16-F1 indicated that the highest activity of curcumin to reduce melanin content of 45,67% at 25 μg/ml. Meanwhile, in the presence of α-MSH at the same concentration indicated that the highest activity was 53,87%. Based on the data, curcumin has potential properties as antioxidant activity and melanin inhibitor.
PENGARUH INFUS RIMPANG TEMULAWAK (Curcuma xanthorrhiza) TERHADAP KADAR HEMOGLOBIN DAN JUMLAH ERITROSIT TIKUS PUTIH YANG DIBERI LARUTAN TIMBAL NITRAT [(PbNO3)2] Sugiharto
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 10 No 1 (2004): December 2004
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23869/419

Abstract

The purpose of this experiment was to study the effect of turmeric (Curcuma xanthorrhiza) rhizome infuse to hemoglobin concentration and number of erythrocyte of [(PbNO3)2] treated rats. Pb was presumed to be link at sulphyhydril groups that may caused an inhibition to several enzymatic process, such as d-ALAD and heme sintetase which is caused the inhibits of Hb synthesis and erythropoesis. Twenty female rats was used in this experiment. They were divided into five groups, i.e. (A) control (treated with 1 ml aquadest); (B) treated with 1/2 ml of 12 ppm lead solution and 1/2 ml aquadest; (C) treated with 1/2 ml of 12 ppm lead solution and 1/2 ml of 20% turmeric rhizome infuse; (D) treated with 1/2 ml of 50 ppm lead solution and 1/2 ml aquadest; (E) treated with 1/2 ml of 50 ppm lead solution and 1/2 ml of 20% turmeric rhizome infuse. The treatment was given orally every day (30 days) using a modified syringe. After 30 days of treatment, the blood sample were taken about 2 ml by heart puncture. The Hb concentration was determined using by Cyanmethemoglobin method and number of erythrocyte was counting on Haemocytometer Improved Neubauer. Data were analyzed by Anova and LSD test (α = 0.05). The results of this study shows that 20% of turmeric rhizome infuse statistically has a significant effect (P < 0.05) to increasing of hemoglobin concentration but hasn’t significant to prevent the number of erythrocyte decreasing of [(PbNO3)2] treated.
The Protective Effect of Gynura procumbens Adventitious Root against Lead Acetate Toxicity in Mice Sugiharto Sugiharto; Dwi Winarni; Ufairanisa Islamatasya; Abdul Hakim Muhsyi; Ahimsa Buena Merpati; Yosephine Sri Wulan Manuhara
Journal of Tropical Biodiversity and Biotechnology Vol 7, No 2 (2022): August
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jtbb.69453

Abstract

Lead induced oxidative stress contributes to increase the productivity of reactive oxygen species (ROS) and to disrupt the antioxidant balance. Gynura procumbens adventitious root (GPAR) methanol extract contains abundant phenolic and flavonoids compounds as antioxidants and can be used as traditional medicinal plants. The objective of this study is to evaluate the protective effect of GPAR against lead acetate toxicity in mice to haematological parameter, histological of hepatic cells, and activities of antioxidant enzymes. The data obtained from five groups of treatment: P1 (control), P2 (Pb acetate-100 mg/L), P3 (GPAR-100 mg/L + Pb acetate-100 mg/L), P4 (GPAR-200 mg/L + Pb acetate-100 mg/L), P5 (GPAR-300 mg/L + Pb acetate-100 mg/L). The results indicated that administration of methanol extract of GPAR can prevent the decreasing of haematological parameter, maintain the percentage of normal hepatic cells, activities of superoxide dismutase (SOD), and catalase (CAT) due to lead acetate treatment. The effective dose of GPAR extract was 300 mg/L. This study provides that methanol extract of G. procumbens adventitious root exerts protective effects against lead acetate toxicity in mice. 
Co-Authors Abdul Hakim Muhsyi Ahimsa Buena Merpati Alfinda Novi Kristanti Anjar Tri Wibowo Arbakariya Ariff Arga Wal Yudha Boon Chin Tan Dwi Winarni Idqa Nurtri Bhakti Muhajir Hamid Nabilatun Nisa R. Djarot Sugiarso Syahida Ahmad Ufairanisa Islamatasya Ufairanisa Islamatasya Yohanes Kartjito Putro Yosephine Sri Wulan Manuhara