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All Journal Jurnal Ilmu Pertanian Jurnal Veteriner JOURNAL OF COASTAL DEVELOPMENT Zuriat Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati Indonesian Journal of Biotechnology Jurnal Pertanian Agros jurnal Ilmu Pertanian (Agricultural Science) Menara Perkebunan Proceeding Biology Education Conference Prosiding SNPBS (Seminar Nasional Pendidikan Biologi dan Saintek)
Sukarti Moeljopawiro
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Education Environmental Science Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Public Health Veterinary

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Kariotip Kromosom Salak , Parjanto; Sukarti Moeljopawiro; W. T. Artama; Aziz Purwantoro
Zuriat Vol 14, No 2 (2003)
Publisher : Breeding Science Society of Indonesia (BSSI) / PERIPI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/zuriat.v14i2.6789

Abstract

Penelitian sitogenetika tanaman salak (Salacca zalacca [Gaertner] Voss) merupakan aspek penting dalam analisis genetika tanaman salak dan menunjang program pemuliaan untuk merakit kultivar salak unggul. Analisis kariotip kromosom salak telah dilakukan dengan metode squash dan pewarnaan acetoorcein. Dengan metode ini, pengamatan morfologi kromosom, yakni panjang dan bentuk kromosom, dapat dilakukan dengan hasil baik pada stadia prometafase. Hasil pengamatan menunjukkan bahwa panjang kromosom salak berkisar 1.15 µm–2.38 µm. Rumus kariotip salak adalah 2n = 28 = 11 m + 1 m (SAT) + 2 sm, yakni terdiri atas 11 pasang kromosom metasentris, 1 pasang kromosom metasentris dengan satelit kromosom, dan 2 pasang kromosom submetasentris. Indeks asimetri kariotip intrakromosomal adalah 0.3 ± 0.03, indeks asimetri inter kromosomal adalah 0.2 ± 0.02. Beberapa pasangan kromosom salak mempunyai bentuk dan ukuran yang mirip sehingga sulit dibedakan. Identifikasi kromosom salak dengan teknik chromosome banding perlu dilakukan untuk mendukung hasil yang telah dicapai.
Cytotoxicity of Buah Merah (Pandanus conoideus Lamk.) Extract on Breast Cancer Cell Line (T47D) Tri R. Nuringtyas; Yoga Pratama; G. Galih; Subagus Wahyuono; Sukarti Moeljopawiro
Indonesian Journal of Biotechnology Vol 19, No 1 (2014)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (246.811 KB) | DOI: 10.22146/ijbiotech.8636

Abstract

Buah Merah (Pandanus conoideus Lamk.) has been extensively used to treat various diseases includingcancer. There are many varieties of buah merah and there was no scientifi c study comparing cytotoxicity ofdifferent varieties. The objective of this study was to investigate the cytotoxicity of three varieties of buah merahknown as Barugum, Maler and Yanggiru on breast cancer cell line (T47D). All samples were collected fromPapua, Indonesia. Each sample was extracted consecutively using three solvents chloroform, methanol andwater resulted to nine crude extracts. The cytotoxic activities were determined using MTT assay. The crudeextract showed the lowest IC50 was selected for further bioassay-guided fractionation. Fractionation was doneusing vacuum liquid chromatography coupled with preparative TLC to fi nd the active compounds. Severaldetection reagents were applied to TLC for identifi cation of the class of the potent compounds. The resultshowed that the potent extracts was obtained from Barugum methanol extract followed by Maler chloroformextract with IC50 value of 132.83 μg/ml and 139.72 μg/ml, respectively. All Yanggiru extracts did not showactivity. The bioassay-guided fractionation of Barugum and Maler extracts showed that the most potent fractioneluted by a mixture of hexane:ethyl acetate (75:25), was in Maler variety with IC50 value of 25,7 μg/ml, fourtimes higher than the most potent fraction of Barugum with IC50 value of 104,61 μg/ml. TLC analysis of themost potent fraction showed that the active compounds was class of terpene. Result of this study supportedthe utilization of buah merah Maler variety for breast cancer treatment.
Kariotipe Kromosom Salak Parjanto Parjanto; Sukarti Moeljopawiro; W.T. Artama; Aziz Purwantoro
Jurnal Ilmu Pertanian Vol 10, No 1 (2003): Juni
Publisher : Faculty of Agriculture, Universitas Gadjah Mada jointly with PISPI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/ipas.60067

Abstract

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Respons Imunoglobulin-G dan Imunoglobulin-M Mencit yang Diberi Ekstrak Methanol Alga Biru Hijau dan Diinfeksi Dengan Takizoit Sorta Basar Ida Simanjuntak; Sukarti Moeljopawiro; Wayan Tunas Artama; Subagus Wahyuono
Jurnal Veteriner Vol 12 No 4 (2011)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (153.616 KB)

Abstract

Toxoplasmosis is an infectious disease caused by Toxoplasma gondii. This disease could severelyaffect humans and animals. Up to now there has been no simple treatment to fight toxoplasmosis. Aprospective alternative treatment to overcome this problem is by increasing immunity of the body using animmunostimulant such as Spirulina platensis. The aims of this research were to observe the potency of S.platensis as an immunostimulant and to find the most potential fraction of S. Platensis that can increasethe responses of IgG and IgM antibodies againts toxoplasma. The responses of these antibodies weremeasured using ELISA method. The isolation of compounds from S. platensis using Preparative ThinLayer Chromatography (PTLC) found three fractions which were a top fraction (I), a middle fraction (II),and a lower fraction (III). Forty-eight mice used in this research were divided into four different groupswith 12 mice in each group and treated differently. The top, middle, and lower fractions of S. platensis wereadministered orally to three groups of mice respectively at dose of 3mg/ml for each mouse while the micein the fourth group were kept as untreated controls. The treatment was conducted for 14 days consecutivelyand on the next day, all mice, including the controls, were challenged with tachizoit. The effect of S.platensisfractions on the responses of IgG and IgM antibodies were then measured at various time intervals, i.e. day0 (before infection) and day 1, 2, and 3 after infection. The results showed that IgG response increased inthe day 0 (2.504 OD) and the day 3 after infection (2.608 OD) while IgM response increased in day 1 afterinfection (2.898 OD). In conclusion, S. platensis was an immunostimulant and the middle fraction (II) of S.Platensis was the most potential fraction to increase immunity againts toxoplasma .
Chitosan Mempertipis Dinding dan Memperbesar Diameter Lumen Arteri Koroner Tikus Putih yang Diberi Pakan Lemak Tinggi (CHITOSAN COULD THINEN WALL AND WIDEN LUMEN DIAMETER CORONARY ARTERY OF SPRAGUE DAWLEY RATS INDUCED BY HIGH FAT RATION) Sri Isdadiyanto; Sukarti Moeljopawiro; Nyoman Puniawati; Hastari Wuryastuty
Jurnal Veteriner Vol 14 No 3 (2013)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (232.052 KB)

Abstract

The objective of this study was to analyze the influence of chitosan on coronary artery wall thicknessand lumen diameter of Sprague Dawley rats induced by high fat ration. The animals for this study weretwenty adult male rats divided into four groups, i.e. group I as the control was fed with basal rationcontaining normal fat for 3 months, group II was fed ration containing high fat for three months, group IIIwas fed ration containing high fat and given chitosan 180 mg per kg body weight per day orally in 2 mLaquadest for 3 months, group IV was fed ration containing high fat for three months and after one monthgiven chitosan 180 mg per kg body weight per day orally in 2 mL aquadest for two months. Each groupconsisted of five animals. After 90 days, the rats were necropsied and the hearts were collected tohistopathological. The difference between treatments was statistical analysis by Anava, and continued byLowest Significant Difference Test to locate the difference. Normal fat ration did not influence coronaryartery wall thickness and coronary artery lumen diameter in rats. High fat ration increased coronaryartery wall thickness and chitosan could be decreased coronary artery wall thickness in rats. High fatration + chitosan that given simultaneously could be increased coronary artery lumen diameter in rats.Based on the result of this study, it was concluded that high fat ration was a major factor able to causeatherosclerosis and chitosan was able to prevent atheroma plaque formation.
THE INFLUENCES OF CHITOSAN FROM Penaeus monodon ON C-REACTIVE PROTEIN EXPRESSION IN AORTA AND CORONARY ARTERY OF SPRAGUE DAWLEY RATS BY HIGH FAT INDUCTION Sri Isdadiyanto; Sukarti Moeljopawiro; Nyoman Puniawati; Hastari Wuryastuty
JOURNAL OF COASTAL DEVELOPMENT Vol 16, No 1 (2012): Volume 16, Number 1, Year 2012
Publisher : JOURNAL OF COASTAL DEVELOPMENT

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (610.937 KB)

Abstract

The objective of this study was to analyze the influences of chitosan on C-Reactive Protein expression in aorta and coronary artery of Sprague Dawley rats by high fat induction. The animals for this study were 20 adult male rats divided into four groups, i.e. group I as the control was fed with basal diet containing normal fat for 3 months, group II was fed diet containing high fat for 3 months, group III was fed diet containing high fat and given chitosan 180 mg per kg body weight per day orally in  2 ml aquadest for 3 months, group IV was fed diet containing high fat for 3 months and  after 1 month given chitosan 180 mg per kg body weight per day orally in 2 ml aquadest for 2 months. Each group consisted of five animals. After 90 days, the rats were necropsied and the hearts were collected to histopathological and immunohistochemical analysis by immunohistochemistry streptavidin-biotin method. C-Reactive Protein expression in aorta was negative. Chitosan was able to prevent atheroma plaque formation in coronary artery and CRP may involve in atherosclerosis.
Pengaruh Pemberian Ekstrak Spirulina platensis terhadap Respon Imun Humoral Mencit setelah Uji Tantang Dengan Takizoit Sorta Basar Ida Simanjuntak; Sukarti Moeljopawiro; Wayan Tunas Artama; Subagus Wahyuono
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 16, No 1 (2011): February 2011
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24002/biota.v16i1.61

Abstract

Toksoplasmosis adalah penyakit yang disebabkan oleh infeksi Toxoplasma gondii. Penyakit ini sangat berbahaya pada hewan maupun manusia. Toksoplasmosis sampai sekarang masih sulit ditanggulangi. Untuk itu, dicari akternatif dengan cara pemberian imunostimulator, seperti Spirulina platensis. Tujuan penelitian ini adalah untuk mengetahui ekstrak Spirulina platensis yang paling potensial meningkatkan respon imun humoral dan mengetahui dosis Spirulina platensis yang efektif dalam meningkatkan respons imun humoral. Rancangan penelitian adalah Rancangan Acak Lengkap pola Faktorial. Faktor pertama adalah jenis ekstrak (ekstrak etil asetat, ekstrak air dan Spirulina platensis murni). Faktor kedua adalah dosis ekstrak (0, 5, 10, 15 mg/ekor mencit). Hasil penelitian menunjukkan bahwa ekstrak etil asetat dengan dosis 5 mg/ekor mencit adalah yang terbaik meningkatkan respon imun humoral. Uji lanjut dengan Latin Square Design (LSD) menunjukkan bahwa terdapat interaksi antara jenis ekstrak dan dosis ekstrak.
INDUKSI PEMBUNGAAN IN VITRO PADA ANGGREK BULAN Phalaenopsis amabilis (L.) Blume INDONESIA IN VITRO FLOWERING OF INDONESIAN Phalaenopsis amabilis (L.) Blume Ixora Sartika Mercuriani; Agus Slamet; Bekti Sulistya Utami; Aries Bagus Sasongko; Aziz Purwantoro; Sukarti Moeljopawiro; Endang Semiarti
Agros Journal of Agriculture Science Vol 16, No 2: Edisi Juli 2014
Publisher : Fakultas Pertanian, Universitas Janabadra

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (146.529 KB)

Abstract

Bunga adalah faktor yang sangat penting dalam budidaya anggrek. Salah satu kendala yang sering dijumpai dalam budidaya anggrek adalah lama fase vegetatif yang dibutuhkan tanaman tersebut untuk dapat berbunga. Pada penelitian ini induksi pembungaan dilakukan pada tahap pertumbuhan awal tanaman secara in vitro. Tanaman umur enam bulan setelah tanam (bst) disubkultur pada medium New Phalaenopsis (NP) dengan konsentrasi Nitrogen (N) setengah dari NP (1/2NP), dengan atau tanpa pemberian Benzyl Adenine (BA), variasi konsentrasi KH2PO4 (1,5 mM dan 3 mM), serta dengan atau tanpa pemotongan akar. Kombinasi perlakuan dengan pemberian BA 22.2 µM, kandungan P tinggi (3 mM), dan pemotongan akar mampu mempercepat pembungaan in vitro anggrek Phalaenopsis amabilis (P. amabilis) asli Indonesia pada umur 18 bst.Flowering is very important in orchid cultivation. However, the long vegetative phase to be able to bloom of the plant becomes an important problem. The orchid needs three up to five years after sowing to bloom. In this study, flowering induction is done in the early growth stages of plants. At six months after sowing (mas), plants were sub-cultured on New Phalaenopsis (NP) medium witha half Nitrogen(N) concentration of NP (1/2NP), with or without Benzyl Adenine (BA), concentration variations of Phosphor/P (1,5 mM and 3 mM),  and with or without roots cutting. In vitro flowering of Indonesian Phalaenopsisamabilis (P. amabilis) can induced on medium that contain 22.2 µM BA and 3 mM P with roots cutting at 18 mas.
OPTIMASI KONDISI FERMENTASI UNTUK PRODUKSI SELULOSA BAKTERI OLEH STRAIN SLK-1 DALAM MEDIA DASAR AIR KELAPA (Optimization Of Fermentation Conditions For The Production Of Bacterial Cellulose By Slk-1 Strain In Coconut Water Based Medium) Sarkono Sarkono; Sukarti Moeljopawiro; Bambang Setiaji; Langkah Sembiring
Proceeding Biology Education Conference: Biology, Science, Enviromental, and Learning Vol 9, No 1 (2012): Prosiding Seminar Nasional IX Biologi
Publisher : Universitas Sebelas Maret

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Abstract

ABSTRAK   Tujuan penelitian ini adalah mengoptimasi kondisi fermentasi terbaik strain Bakteri Asam Asetat penghasil selulosa yaitu isolat SLK-1.  Strain ini diisolasi dari buah salak pada penelitian sebelumnya.  Hasil optimasi menunjukkan bahwa kondisi fermentasi optimum untuk pertumbuhan dan produksi selulosa pada isolat SLK-1  dicapai dengan sumber karbon gula pasir, sumber nitrogen ammonium sulfat, pH 7, suhu inkubasi 25°C dan metode fermentasi statis. Karakter struktur permukaan selulosa hasil fermentasi isolat SLK-1 dipengaruhi oleh metode fermentasi yang digunakan.  Metode fermentasi goyangan berpengaruh menurunkan produksi selulosa pada  isolat SLK-1 dan merubah struktur permukaan yaitu susunan mikrofibril lebih renggang dan membentuk gelembung.   Kata Kunci: bakteri asam asetat, optimasi, fermentasi, selulosa bakteri, penggoyangan
Kariotipe Kromosom Salak Parjanto Parjanto; Sukarti Moeljopawiro; W.T. Artama; Aziz Purwantoro
Jurnal Ilmu Pertanian Vol 10, No 1 (2003): Juni
Publisher : Faculty of Agriculture, Universitas Gadjah Mada jointly with PISPI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/ipas.60067

Abstract

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Co-Authors , Parjanto Agus Slamet Agustina A. HANDAYAN Aries Bagus Sasongko Aziz Purwantoro Bambang Setiaji Bekti Sulistya Utami Djoko Santoso ENDANG S. SOETARTO Endang Semiarti G. Galih Hastari Wuryastuty Ixora Sartika Mercuriani Langkah Sembiring Nyoman Puniawati Parjanto Parjanto Sarkono Sarkono Sorta Basar Ida Simanjuntak Sorta Basar Ida Simanjuntak Sri Isdadiyanto Sri Isdadiyanto Subagus Wahyuono Tjut S. Djohan Tri R. Nuringtyas W. T. Artama W.T. Artama Wayan Tunas Artama Yoga Pratama Yuliana Retnowati