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All Journal Jurnal Sain Veteriner Buletin Peternakan Buletin Veteriner Udayana Jurnal Veteriner BIOTROPIA - The Southeast Asian Journal of Tropical Biology Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati Jurnal Informatika dan Rekayasa Elektronik
Wayan Tunas Artama
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Computer Science & IT Immunology & microbiology Medicine & Pharmacology Public Health Veterinary Other

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ANALISA VISUAL MENGGUNAKAN ETETOOLKIT FRAMEWORK TERHADAP PENYAKIT BETA-THALASSEMIA DI JAWA TENGAH BAGIAN SELATAN Lalu Mutawalli; Moh Reza Syaifur Rizal; Wayan Tunas Artama; Rohmatul Fajriyah; Izzati Muhimmah; Lantip Rujito
Jurnal Informatika dan Rekayasa Elektronik Vol. 2 No. 1 (2019): JIRE April 2019
Publisher : LPPM STMIK Lombok

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36595/jire.v2i1.75

Abstract

Deteksi peristiwa biomolekuler dalam visual yang akan dianalisis menggunakan komputasi untuk mendeteksi efektivitas dan akurasi penyakit. Sebagai hasil utama, banyak analisis visual, mulai dari pengelompokan gen hingga filogenetik, menghasilkan pohon hierarkis. Toolkit Lingkungan Eksplorasi Pohon (ETE) yang membantu manipulasi, analisis, dan visualisasi pohon hierarkis otomatis. Kemudian, dalam makalah ini, daftar mutasi β-thalassemia yang merupakan kelompok kelainan darah herediter yang ditandai oleh anomali dalam sintesis rantai beta hemoglobin yang menghasilkan berbagai fenotipe mulai dari anemia berat hingga individu tanpa gejala klinis. Hasil ini adalah ETEToolkit dapat menguraikan mutasi ini untuk ditampilkan melalui pohon dan penyelarasan dalam satu bingkai, kemudian kita dapat menyesuaikan dan merender ke dalam gambar PDF. Mutasi ini berlokasi di pusat Jawa, Indonesia.
KARAKTERISASI ANTIBODI MONOKLONAL TERHADAP PROTEIN MEMBRAN Toxoplasma gondii ISOLAT LOKAL Rini Widayanti; Widya Asmara; Wayan Tunas Artama
Jurnal Sain Veteriner Vol 22, No 1 (2004): Juli
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1255.568 KB) | DOI: 10.22146/jsv.443

Abstract

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Kajian Molekuler Daerah D-Loop Parsial Deoxyribonucleic acid (DNA) Mitokondria Kuda (Equus caballus) Asli Priangan Yuriadi .; Rini Widayanti; Aris Haryanto; Wayan Tunas Artama
Jurnal Sain Veteriner Vol 27, No 2 (2009): DESEMBER
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1948.518 KB) | DOI: 10.22146/jsv.463

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Deteksi Genotipe K-Kasein dengan Teknik Polymerase Chain Reaction-Single Strand Conformational Polymorphism dan Efek Genetiknya terhadap Kadar Protein dan Lemak Susu Kambing Peranakan Etawah Maria Astuti; Wayan Tunas Artama; Muladno (Muladno); Yustina Yuni Suranindyah
Buletin Peternakan Vol 28, No 1 (2004): Buletin Peternakan Vol. 28 (1) Februari 2004
Publisher : Faculty of Animal Science, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21059/buletinpeternak.v28i1.1487

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ISOLASI TOXOPLASMA GONDII PADA AYAM BURAS Ida Ayu Pasti Apsari; Wayan Tunas Artama; Sumartono -; I Made Damriyasa; Ida Bagus Made Oka; Ida Bagus Ngurah Swacita
Buletin Veteriner Udayana Vol. 3 No.2 Agustus 2011
Publisher : The Faculty of Veterinary Medicine, Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (87.902 KB)

Abstract

A study was conducted Toxoplasma gondii isolate from the brain and the heart of freerangechickens in Bali. The aim of this study to determine the seroprevalence andto isolate Toxoplasma gondii from free-range chicken heart and brain. To achieve of thisstudy observed 311 free-range chicken serum samples with ELISA method and examined225 free-range chicken brain and heart used digestion method. All of the samples takenfrom 9 districts in Bali. The results showed that the seroprevalence of Toxoplasma gondiiinfreerange chicken has 91.64%. Isolation of Toxoplasma godii from the heart and the brainfreerange chicken found the cyst on inoculate heart and brain, but by bioassay in mice for4 weeks observation failed to find tachyzoite form in peritonial exudat
Identifikasi Escherichia coli O157:H7 serta Deteksi Gen Shiga Like Toxin 1 dan 2 Asal Feses Hewan, Daging, dan Feses Manusia I Wayan Suardana; Wayan Tunas Artama; Widya Asmara; Budi Setiadi Daryono
Jurnal Veteriner Vol 11 No 4 (2010)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (196.104 KB)

Abstract

Escherichia coli O157:H7 with the ability to produce shiga-like toxin was isolated from beef, cattle,chicken, and human feces. Due to its importance to human health, it is necessary to identify the genesencoding the production of shiga-like toxin, stx1 and stx2 respectively to further understand the pathogenesis.Isolation of E. coli was done on Eosin Methylene Blue Agar (EMBA), followed by identification on SorbitolMacConkey Agar (SMAC), latex agglutination test, and H7 antiserum test, respectivelly. The existence ofgenes stx1 and stx2 in E. coli O157:H7 was confirmed molecularly using PCR method with specific primersLP 30/31 and LP 43/44, Stx2 (F)/Stx2 (R) respectively. Escherichia coli O157:H7 was isolated from 22 outof 344 samples (6,4%). Some isolates showed gene stx1 and stx2 was detected in two isolates as indicatedby a 384 bp band (stx1 gene), 584 bp and 1588 bp bands (stx2 gene) respectivelly. The results indicatedthat local isolates E. coli O157:H7 are potential as a zoonoses agent.
Pelacakan Protein Wingless-Type MMTV Integration Site Family Member-4 pada Uterus Mencit (DETECTION WINGLESS-TYPE MMTV INTEGRATION SITE FAMILY MEMBER-4 PROTEIN OF MOUSE UTERUS) Agung Janika Sitasiwi; Wayan Tunas Artama; Agung Budiyanto; Edy Dharmana
Jurnal Veteriner Vol 17 No 1 (2016)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (136.168 KB)

Abstract

The aims of this study was to detect the expression of Wingless-type MMTV integration site familymember 4 (Wnt4) protein in the uterus of Swiss Webster mice. Laboratory animals that were used areadult Swiss Webster mice weighing 25-30 grams. Mice were kept and mated in a controlled laboratoryconditions. Pregnancy was determined by the presence of vaginal plug in female mice after breeding.Protein was isolated from the uterus at seven days of gestation. Immunoblotting was performed usingChemiluminescent Western Blot kit. The primary antibody used was anti Wnt-4 antibody with a 1: 1000dilution. The results showed protein bands with molecular weights ranging from 40 kDa showed a positivereaction to the primary antibody that were used so that it can be concluded that the protein is Wnt4protein.
Analisis Sekuen Probe Gena Shiga Like Toxin-2 dari Isolat Lokal Escherichia coli O157:H7 (PROBE SEQUANCE ANALYSIS OF SHIGA LIKE TOXIN-2 GEN FROM ESCHERICHIA COLI O157:H7 LOCAL ISOLATES) I Wayan Suardana; I Nengah Sujaya; Wayan Tunas Artama
Jurnal Veteriner Vol 14 No 2 (2013)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (580.12 KB)

Abstract

A Shiga-like toxin producing Escherichia coli O157:H7 was detected in faecal samples of cattle, andhuman as well as in beef. The performance of agent indicated that it has been identified as harmful andoften life-threatening zoonotic agent. It is therefore important to analysed the genetic characteristic ofShiga toxin Escherichia coli (STEC) and to develop a diagnostic probe in order  to optimalized of diagnostictest  for the agent. The  study was started by amplifiying  stx2 gene, purifying of PCR product, sequencingof stx2 gene, analyzing  of phylogenetic tree, and finally  by analyzing   of  diagnostic  probe candidate.Homology study showed that the genetic sequence of the local isolate of  E. coli O157:H7 i.e SM25(1)isolated from cattle feces has  a genetic and fuctional similarity with  the control isolate i.e E. coli O157:H7ATCC 43894 originated from human.  Further study showed that a probe with  foreward primer  sequanceof 5’-AATTTATATGTGGCCGGGTTC-‘3 which were respectively designed as a PFS and PRS 176 bp product.Appeared to be potential candidate of diagnostic probe for the agent.
Virgin Coconut Oil Meningkatkan Aktivitas Fagositosis Makrofag Ayam Pedaging Pascavaksinasi Flu Burung (VIRGIN COCONUT OIL INCREASES THE PHAGOCYTOSIS ACTIVITY OF MACROPHAGE OF BROILER CHICKEN FOLLOWING AVIAN INFLUENZA VACCINATION) Enny Yusuf Wachidah Yuniwarti; Widya Asmara; Wayan Tunas Artama; Charles Rangga Tabbu
Jurnal Veteriner Vol 14 No 2 (2013)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (135.108 KB)

Abstract

The research objective was to find an alternative avian influenza prevention in broilers by increasinganimal’s antibody titer and macrophages phagocytic  activity.  Virgin coconut oil (VCO) is a food supplementthat is proven safe for human consumption therefore it is assumed to be safe for the animal’s (chickens).Factorial design  2 vaccinated: unvaccinated) x 4 (dose of VCO: 0, 5, 10 and 15 mL/kg feed) were applied inthis study.  A total of 40 day day old chick were allocated in the eight treatments groups.  Feed and drinkingwater were available  ad libitum.  Antibody titers of the animals were detected using ELISA, whereasphagocytic activity of the macrophages were detected from spleen.  The result showed that the highestphagocytic activity and antibody titers were seen in chickens which were given VCO at 10 mL/kg feed.  It isconcluded that the VCO could increased the phagocytic activity of macrophages.
Studi Epidemiologi Agen Zoonosis Escherichia coli O157:H7 melalui Analisis Random Amplification of Polymorphic DNA (RAPD) I Wayan Suardana; Wayan Tunas Artama; Widya Asmara; Budi Setiadi Daryono
Jurnal Veteriner Vol 12, No 2 (2011)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (264.692 KB)

Abstract

Epidemiological studies of zoonotic agent Escherichia coli O157:H7 have been analyzed pheneticallyand or phylogenetically. In a phenetic classification, micoorganisms are arranged into groups (phena) onthe basis of high overall similarity using both phenotypic and genotypic methods without judgementaspect of its ancestry or evolutionary. Due to its importance to epidemiological aspect, the study of geneticvariation of isolates origin from some sources need to be conducted in order to trace the routes of infection.A total of 20 samples obtained from some sources i.e clinically human feces, non-clinically human feces,cattle feces, chicken feces, and beef feces were used in this study. The study was started by confirming allof the isolates using O157 latex agglutination test and H7 antiserum test, followed by genomic DNAanalysis by random amplification of polymorphic DNA /RAPD methods. RAPD results were analyzed using a simple matching coeficient (Ssm) and alogorhythm unweighted pair group method using arithmeticaverages (UPGMA) programe. Results showed there were range of genetic DNA from local isolates (75.1–99,6%) which was almost similar to ATCC 43894 control isolate. The highest similarity (99.6%) to ATCC43894 control was showed by SM-7(1) isolate obtained from cattle fecal and KL-68(1), isolate obtainedfrom clinically human fecal. In addition, KL-52(7) obtained from clinically human fecal had high similarity(99.6%) to MK-35 isolate obtained from chicken fecal. On the other hand, DS-21(4) and DS-16(2) isolatesthat were obtained from beef had high similarity (84.9%) to other isolates including ATCC 43894 controlisolate. The highest similarity of E. coli O157:H7 isolates that were obtained from cattle feces, beef, andchicken feces to human feces isolate indicated that there were both cattle and chicken were potentialreservoirs of the zoonotic agen which can be transmitted to human.
Co-Authors Agung Budiyanto Agung Janika Sitasiwi Aris Haryanto Asih Kurniawati BUDI SETIADI DARYONO Charles Rangga Tabbu Dewa Ayu Agus Sri Laksmi Edy Dharmana Enny Yusuf Wachidah Yuniwarti I Made Damriyasa I Nengah Sujaya I Wayan Suardana Ida Ayu Pasti Apsari Ida Bagus Made Oka Ida Bagus Ngurah Swacita Izzati Muhimmah Lalu Mutawalli Lantip Rujito Lies Mira Yusiati Mahardika Agus Wijayanti Maria Astuti Moh Reza Syaifur Rizal Rini Widayanti Rini Widayanti Rohmatul Fajriyah Saiful Akhyar Lubis Sorta Basar Ida Simanjuntak Sorta Basar Ida Simanjuntak Subagus Wahyuono Sukarti Moeljopawiro Sumartono - Sumartono . Trini Susmiati Widya Asmara Yuriadi . Yustina Yuni Surandiyah