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Mukhamad Nur Khamid
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ANTIBACTERY ACTIVITIES OF ETHANOL EXTRACT 70% KERSEN LEAVES (Muntingia calabura L.) ON THE BACTERIA Salmonella typhi Ni Ima Wuri Handayani; Mukhamad Nur Khamid; Ambar Yunita Nugraheni
STIKES DUTAGAMA KLATEN Vol 9 No 2 (2017): JURNAL ILMU KESEHATAN STIKES DUTA GAMA KLATEN
Publisher : STIKES DUTAGAMA KLATEN

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5737/v9i2.261

Abstract

Background: Typhoid fever is infectious disease caused by Salmonella typhi. The activity of Salmonella typhi can be blocked by antibacterial. Extracts of cherry leaves (Muntingia calabura L.) can be used for alternative medication because it contains of active substance as antibacterial. Objective: This research was aimed to investigate the antibacterial activity of 70% ethanol extracts of cherry leaves in resisting Salmonella typhi. Method: Cherry leaves were extracted by maceration method using 70% ethanol. The extract was determined by phytochemical screening using tube test. The determination of antibacterial activity uses Mueller Hinton which was done by Cup-Plate method. This research used various concentration such as 5.3% b/v, 17.6% b/v, 33.3% b/v and 53.8% b/v. Thick extract of cherry leaves was used as extract control, positive control used 250% of chloramphenicol while negative control used 250% of CMC-Na. Result: The result of the research showed that cherry leaves contain flavonoids, tannins, polyphenols and saponins. Minimum Inhibitory Concentration (MIC) toward Salmonella typhi is found at the concentration of 33,3% b/v in the 11,7 mm average of diameter inhibition zone. Conclusion: 70% ethanol extracts of cherry leaves has antibacterial activity in resisting Salmonella typhi.
IDENTIFIKASI CEMARAN SALMONELA Sp. PADA DAGING AYAM BROILER DI WILAYAH PEDAN KABUPATEN KLATEN Akrom Basharudin; Mukhamad Nur Khamid; Waskitho .
STIKES DUTAGAMA KLATEN Vol 8 No 1 (2016): JURNAL ILMU KESEHATAN STIKES DUTA GAMA KLATEN
Publisher : STIKES DUTAGAMA KLATEN

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5737/v8i1.336

Abstract

Latar Belakang : Salmonella merupakan bakteri gram-negatif, tidak berspora, tidak mempunyai simpai, tanpa fimbria dan mempunyai flagel peritik, kecuali Salmonella pullorum dan Salmonella gallinarum. Salmonella masuk kedalam tubuh melalui makanan atau minuman yang terkontaminasi. Salmonella Sp. dikenal sebagai bakteri penyebab salmonellosis. Untuk menyegah penyebaran Salmonella Sp. diperlukan identifikasi keberadaan cemaran Salmonella Sp. Tujuan Penelitian : Untuk mengetahui keberadaan cemaran bakteri Salmonella Sp. pada daging ayam broiler di beberapa tempat penjual daging ayam broiler di wilayah Pedan, Kabupaten Klaten. Metode Penelitian : Penelitian ini merupakan penelitian non eksperimental desain cross-sectional. Sampel diambil di 3 lokasi pasar di wilayah Pedan Kabupaten Klaten yaitu pasar Keden, pasar Temuwangi, dan pasar Kedungan. Masing-masing lokasi diambil 1sampel. Sampel ditumbuhkan dalam medium Lactose Broth (LB), diiinkubasi pada suhu 35°C ± 1°C selama 24 jam. Kultur dari medium lactose Broth kemudian di pindah pada medium Rappavort Vasilidiasis (RV) untuk menghambat bakteri selain Salmonella, diiinkubasi pada suhu 35°C ± 1°C selama 24 jam, tahap berikutnya goreskan pada media Bismuth Sulfite Agar (BSA) diinkubasi selama 24 jam pada suhu 35°C. Hasil Penelitian : Menunjukkan bahwa 3 sampel yang diperoleh dari pasar Keden, Temuwangi, dan Kedungan positif tercemar bakteri Salmonella Sp. Kesimpulan : Hasil identifikasi cemaran Salmonella Sp. pada daging ayam broiler di wilayah Pedan Kabupaten Klaten menunjukan bahwa semua sampel positif tercemar bakteri Salmonella Sp.
PENGARUH ASUPAN JUS DAUN KATUK SEGAR TERHADAP VOLUME ASI PADA IBU MENYUSUI SAAT SUHU BASAL DI DESA DEWAN KETAON BANYUDONO BOYOLALI Yunita Selvianingrum; Titis Sensussiana; Mukhamad Nur Khamid
STIKES DUTAGAMA KLATEN Vol 7 No 2 (2015): JURNAL ILMU KESEHATAN STIKES DUTA GAMA KLATEN
Publisher : STIKES DUTAGAMA KLATEN

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5737/v7i2.353

Abstract

Background: Program the gift ASI do not always go well one of constraint is decrease it produce the ASI. Some factor influencing efficacy produce the ASI of Asupan gizi, food, emotion, physiology, intrauterine device influence. Between above factor of asupan gizi mother very having an in with of efficacy produce the ASI. One of well-balanced gizi during suckling with consuming vegetable vegetable. From some the vegetable of leaf katuk trusted and have is often used by a society to increase volume ASI. Leaf of Katuk containing of is existence of polifenol and steroid which playing a part in of reflek prolaktin to produce the ASI and also can stimulate the hormone oksitosin. Pursuant to data obtained from profile of health of provinsi of Central Java in the year 2010 showing coverage ASI only about 37,18 % from totalizeing baby amount that is 488.495. Research Purposes: To know is there any influence of asupan of fresh leaf katuk juice to volume ASI of at mother suckle in Countryside of Council of Ketaon Banyudono Boyolali Research Method: This Research use the method of research of kuasi eksprerimen (Experiment Pre) where Variable independent in this research is asupan of fresh leaf katuk juice and Variable dependent in this research is volume ASI of at mother suckle the moment of dropsy temperature. Sampel taken by as much 26 respondek with the total technique of sampling. Data obtained to be analysed to use the statistical test of T-Test. Research Result: Statistical Test of t-test got by result of volume ASI of before, moment, pasca treatment assess = 0,000 (ρ<0,05) with the level signifikansi 5%. Conclusion: There is influence of fresh leaf katuk juice to volume ASI of at mother suckle the moment of dropsy temperature. With the value = 0,000 (ρ<0,05) hence Ho refused and is Ha accepted.
PENGARUH PERASAN LABU SIAM TERHADAP PERUBAHAN TEKANAN SISTOLIK DAN DIASTOLIK DI DUKUH WONOREJO DESA TEMUWANGI KECAMATAN PEDAN Ernawati .; Mukhamad Nur Khamid; Yeni Rusyani
STIKES DUTAGAMA KLATEN Vol 7 No 2 (2015): JURNAL ILMU KESEHATAN STIKES DUTA GAMA KLATEN
Publisher : STIKES DUTAGAMA KLATEN

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5737/v7i2.355

Abstract

Background: Primary hypertension is 90% of the hypertension sufferers cases. In the longterm, if it is not cured immediately, it will lead artery’s damage in the body up to the organ which gets the blood supply from the artery and complication of the other organs will occur. Chayote is a vegetable commonly consumed by people to lower the blood pressure. Objective: To identify the influence of chayote’s extract towards the change of the systolic and diastolic pressures of hypertension’s sufferers in Wonorejo, Temuwangi village, Pedan sub district. Method: Experimental design with time series method. This research was done in nine days which consisted of three days for observation before the treatment, three days for the treatment, and three days after the treatment. The extract of chayote as much as 200ml as given twice per day in the morning and in the afternoon and afterwards the measurement of the systolic and diastolic pressure during the nine days as done once every morning. There were twenty five respondents with total sampling method. The date was processed using parametric statistic tests with t-test technique: paired two samples for means. Results: There was the change of systolic and diastolic pressure. Before the treatment, the average of systolic pressure had been 158.32 mmHg. During the treatment, the average of systolic pressure was 146.20 mmHg. After the treatment, the average systolic pressure was 132.32. Mean while, the average of diastolic pressure before the treatment had been 82.28 mmHg. During the treatment, the average of systolic pressure was 79.20 mmHg. After the treatment, the average of systolic pressure was 70.80 mmHg. Conclusion: The extractof chayote affected the change of systolic and diastolic pressures of primary hypertension’s sufferers in Wonorejo, Temuwangi village, Pedan sub-district.
UJI AKTIVITAS ANTIBAKTERI EKSTRAK ETANOL 96% BUAH OKRA (Abelmoschus esculentus L.) TERHADAP BAKTERI Escherichia coli Ardiani Cahyaningrum; Mukhamad Nur Khamid; Muhammad Nurhadi
STIKES DUTAGAMA KLATEN Vol 10 No 2 (2018): JURNAL ILMU KESEHATAN STIKES DUTA GAMA KLATEN
Publisher : STIKES DUTAGAMA KLATEN

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5737/v10i2.432

Abstract

disebabkan Virus, Bakteri, dan Parasit. Bakteri yang sering menyebabkan diare dengan presentasi yang banyak terjadi pada Escherichia coli. Aktivitas bakteri Escherichia coli dapat dihambat dengan antibakteri. Buah Okra (Abelmoschus esculentus L.) diduga dapat digunakan sebagai pengobatan alternatif karena mengandung flavonoid yang merupakan kelompok senyawa polifenol yang berfungsi sebagai antibakteri. Tujuan: Penelitian ini bertujuan untuk mengetahui aktivitas antibakteri ekstrak etanol 96% Buah Okra (Abelmoschus esculentus L.) terhadap bakteri Escherichia coli. Metode Penelitian: Penelitian ini merupakan penelitian eksperimental menggunakan Posttest Only Control Group Design. Buah Okra diekstraksi dengan metode maserasi menggunakan pelarut etanol 96%, kemudian diuapkan menggunakan rotary evaporator sehingga didapat ekstrak kental. Ekstrak yang diperoleh selanjutnya dilakukan skrining fitokimia dengan uji tabung. Uji aktivitas antibakteri menggunakan media Mueller Hinton dengan metode sumuran. Konsentrasi ekstrak yang digunakan yaitu 20%, 40%, 60%,80%, dan 100%. Kontrol positif menggunakan kloramfenikol sedangkan kontrol negatif menggunakan aquades. Hasil: Hasil penelitian menunjukan bahwa tidak ada zona hambat. Kesimpulan: Ekstrak Etanol 96% Buah Okra tidak memiliki aktivitas antibakteri terhadap Escherichia coli yang ditandai dengan tidak adanya zona hambat pada sekitar lubang sumuran. Hal ini disebabkan hilangnya senyawa antibakteri yang dibuktikan dengan skrining fitokimia menunjukan hasil negatif. Dikarenakan tidak adanya zona hambat pada sekitar lubang sumuran, maka tidak diketahui berapa kosentrasi hambat minimumnya
ANALISIS KANDUNGAN FLAVONOID EKSTRAK ETANOL 70% SIMPLISIA DAUN UNGU (Graptophyllum pictum L griff.) DENGAN METODE KROMATOGRAFI LAPIS TIPIS DENSITOMETRI Zidny Aulia; Mukhamad Nur Khamid; Mitta Aninjaya
STIKES DUTAGAMA KLATEN Vol 10 No 2 (2018): JURNAL ILMU KESEHATAN STIKES DUTA GAMA KLATEN
Publisher : STIKES DUTAGAMA KLATEN

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5737/v10i2.433

Abstract

Latar Belakang: Daun ungu berkhasiat sebagai obat yang memiliki kandungan kimia antara lain alkaloid non toksik, saponin, antosianin, flavonoid, alkohol dan lain-lain. Flavonoid di analisis dengan analisa kualitatif menggunakan KLT-Densitometri. Tujuan: Mengetahui jenis Flavonoid ekstrak etanol 70% simplisia daun ungu (Graptophyllum pictum L griff.) menggunakan metode KLT-Densitometri Metode Penelitian: Penelitian ini merupakan penelitian eksperimental menggunakan disain Deskriptif. Serbuk daun ungu diekstraksi dengan metode maserasi menggunakan pelarut etanol 70%, ekstrak yang didapat diuji skrining fitokimia dengan uji tabung analisis jenis flavonoid daun ungu dengan metode KLT-Densitometri. Hasil: Hasil penelitian menunjukkan bahwa daun ungu mengandung Flavonoid dan jenis senyawa Flavonoid adalah rutin, heperoside, dan quercetin. Kesimpulan: Terdapat jenis Flavonoid 300 ppm didapatkan jenis rutin, 200 ppm jenis heperoside dan quercetin dan 100 ppm jenis rutin.
Co-Authors Akrom Basharudin Ambar Yunita Nugraheni Ardiani Cahyaningrum Ernawati . Mitta Aninjaya Muhammad Nurhadi Ni Ima Wuri Handayani Titis Sensussiana Waskitho . Yeni Rusyani Yunita Selvianingrum Zidny Aulia