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PENGARUH PEMANASAN BERBASIS GELOMBANG MIKRO DALAM PROSES EKSTRAKSI ENZIMATIS VANILIN PADA POLONG VANILA Vita Paramita; Wahyuningsih Wahyuningsih; Mohammad Endy Yulianto; Oktavian Dita Ratnasari
Prosiding SNST Fakultas Teknik Vol 1, No 1 (2016): PROSIDING SEMINAR NASIONAL SAINS DAN TEKNOLOGI 7 2016
Publisher : Prosiding SNST Fakultas Teknik

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (315.163 KB)

Riset ini bertujuan untuk mengembangkan skema proses ekstraksi enzimatis untuk produksi polong vanila terhidrolisa efisien waktu dan energi. Target yang ingin dicapai berupa optimisasi parameter proses. Kajian dilakukan pada berbagai variabel,meliputi suhu (30 - 50 0C), tipe enzim (protease dan selulase) dengan rasio enzim-substrat 1:50, pH 4,5. Selama tempuhan, diukur kdar senyawa vanilin dengan alat spektrofotometer ultra violet, sebagai fungsi waktu. Penentuan variabel yang berpengaruh dapat menggunakan central composite rotatotional design (CCRD). Percobaan menunjukkan bahwa nilai vanilin optimum mencapai lebih dari 400 mg/L diperoleh pada suhu hidrolisa-ekstraksi antara 50-55 0C dengan lama waktu antara 55-60 menit. Kata kunci: ekstraksi, gelombang mikro, polong vanila, vanilin

Utilization of Rambutan (Naphelium lappaceum L) Peel’s Waste is Becoming More Useful As a Natural Dye of Fabrics Heny Kusumayanti; Vita Paramita; Rizka Amalia; Wahyuningsih Wahyuningsih
Waste Technology Vol 8, No 1 (2020)
Publisher : Diponegoro University

The demand to find an alternatives source of fabric dyes is increasing nowadays, specifically for natural dye. Applying natural dye in fabric, giving important effect of more environmentally friendly than synthetic dye. One of the natural colors that can be used is Naphelium lappaceum L peel’s waste. The method used is dyeing a cloth with extract of Naphelium lappaceum L peels. Variables applied are extract concentration, type of fixation and concentration of fixation. The results of dyeing cotton fabric using Naphelium lappaceum L peel extract show that the use of alum solution as fixation materials provides brown light color, yellowish brown for lime, and blackish grey for ferro sulphate. The different extract concentration resulted significant effect on the colour radiance on fabric.

THE STUDY ON THE EFFECT OF PROCESS VARIABLES TOWARDS BIOADSORBENT PERFORMANCE OF MODIFIED PALM BUNCH FOR THE REDUCTION OF 3-MCPD ON THE PALM OIL Wahyuningsih Wahyuningsih; Mohamad Endy Yulianto; Rizka Amalia; Eflita Yohana
Waste Technology Vol 5, No 2 (2017)
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/5.2.%p

Crude palm oil was consumed as a healthy food oil, it contains a 3-monochloro-propane-1,2-diol (3-MCPD) ester in the range of 0.04-0.05 ppm. The 3-MCPD compound is one of the contaminants belonging to the chloropropanol group that is genotoxin carcinogen. The 3-MCPD ester content specification in palm oil is still a polemic, but some EU countries already use it as a barrier of palm oil trade in the European region. It is therefore necessary to develop an integrated palm oil refining through adsorption with a modified palm empty fruit bunch bioadsorbent to reduce 3-MCPD ester (<0.02 ppm / Codex Standard).

The Application of Functional Theory: Indonesian Presidential Debate Wahyuningsih Wahyuningsih; Deli Nirmala
ASIAN TEFL Journal of Language Teaching and Applied Linguistics ASIAN TEFL: Journal of Language Teaching and Applied Linguistics, VOL 5(1), 2020
Publisher : Lecturer Association of Linguistics, Language Teaching, and Literature Studies in Indonesi

Functional theory is a one of theories that are common used on the research of political debates in American campaign discourse. In the present article, Indonesian presidential debate is analyzed from the perspective of functional theory. The goal of this study is to analyze language function produced by candidates and to evaluate the applicability of functional theory in analyzing Indonesian presidential debate. The authors employ qualitative method in analyzing data. Supporting instruments in analyzing data were functional theory and political discourse analysis (PDA). PDA used in interpreting language function in political context. While, functional theory provides three functions, namely: attacks, defends, and acclaims. The finding shows that two different axioms are found. The first is the use of attack more than defense; it is line with the prediction of functional theory that has been tested in American political debate. Second, the use of acclaims less than attack, it differs in a way from the prediction of functional theory. Fundamentally, functional theory can be used as an instrument in analysis political debates in Indonesia even though the findings were not exactly consistence with the prediction. Thereby, the theory needs to be developed discursively.

ASIDOLISIS ENZIMATIK MINYAK IKAN TUNA( THUNNUS THYNNUS) MENJADI PRODUK ASAM LEMAK KAYA OMEGA-3 DENGAN PEMANFAATAN LIPASE GETAH PEPAYA (carica papaya latex) Wahyuningsih Wahyuningsih; Isti Pudjihastuti; Heny Kusumayanti
METANA Vol 7, No 01 (2011): Juli 2011
Publisher : Universitas Diponegoro

ABSTRACT Incorporation of omega-3 polyunsaturated acids (n-3 PUFA) into Tuna (Thunnus thynnus) fish oil was investigated by using acidolysis enzimatis Process. The product of this modification is expected to be used as an ingredient nutrifition food products, especially in milk and baby food as well as pregnant and lactating The purpose of this study was to optimizing the used of lipased papaya latex (Carica papaya latex) in the product to incorporation fatty acids rich in omega-3.Bioreactor acidolysis enzematik development and Optimization of process the productivity of fatty acids rich in omega-3. Reaction Acidolysis was conducted between tuna (thunnus thynnus) fish oil by microbial lipase (5% of the weight of the substrate mixture) or vegetable lipased (Carica papaya latex) (6-10% by weight subtract mixture) as biocatalist.Acidolysis mixture the bioreactor at 40 o C for (2-6 hours) and the stirring speed of 200 rpm, pH (4.5 to 6.5). to eliminate free fatty acids from the product acidolysis performed neutralization with NaOH The results are expected to show that the lipase papaya latex (Carica papaya latex) can be used as biocatalyst incorporated omega-3 fatty acids in tunaIthunnus thunnyl) fish oil . In this study sought conditions for optimum incorporation rate, ie the use of papaya latex lipase concentration of 8%, the ratio of concentrations of omega-3 fatty acid and palm oil (1:1), pH = 5.5, Optimal time of 4 hours and the temperature (40oC) . The results were analyzed incorporation of omega-3 (EPA and DHA) with GC.EPA generated: 3.57% and DHA = 3.91% Key word:Acidolysis,Tuna fish oil

ASIDOLISIS ENZIMATIK MINYAK IKAN TUNA( THUNNUS THYNNUS) MENJADI PRODUK ASAM LEMAK KAYA OMEGA-3 DENGAN PEMANFAATAN LIPASE GETAH PEPAYA (carica papaya latex) Wahyuningsih Wahyuningsih; Isti Pudjihastuti; Heny Kusumayanti
METANA Vol 8, No 01 (2012): Juli 2012
Publisher : Universitas Diponegoro

Incorporation of omega-3 polyunsaturated acids (n-3 PUFA) into Tuna (Thunnus thynnus) fish oil was investigated by using acidolysis enzimatis Process. The product of this modification is expected to be used as an ingredient nutrifition food products, especially in milk and baby food as well as pregnant and lactating The purpose of this study was to optimizing the used of lipased papaya latex (Carica papaya latex) in the product to incorporation fatty acids rich in omega-3.Bioreactor acidolysis enzematik development and Optimization of process the productivity of fatty acids rich in omega-3. Reaction Acidolysis was conducted between tuna (thunnus thynnus) fish oil by microbial lipase (5% of the weight of the substrate mixture) or vegetable lipased (Carica papaya latex) (6-10% by weight subtract mixture) as biocatalist.Acidolysis mixture the bioreactor at 40 o C for (2-6 hours) and the stirring speed of 200 rpm, pH (4.5 to 6.5). to eliminate free fatty acids from the product acidolysis performed neutralization with NaOH The results are expected to show that the lipase papaya latex (Carica papaya latex) can be used as biocatalyst incorporated omega-3 fatty acids in tunaIthunnus thunnyl) fish oil . In this study sought conditions for optimum incorporation rate, ie the use of papaya latex lipase concentration of 8%, the ratio of concentrations of omega-3 fatty acid and palm oil (1:1), pH = 5.5, Optimal time of 4 hours and the temperature (40oC) . The results were analyzed incorporation of omega-3 (EPA and DHA) with GC.EPA generated: 3.57% and DHA = 3.91% Key word:Acidolysis,Tuna fish oil

Produksi Sabun Cuci Piring Sebagai Upaya Peningkatkan Efektivitas Dan Peluang Wirausaha Rizka Amalia; Vita Paramita; Heny Kusumayanti; Wahyuningsih Wahyuningsih; Maranatha Sembiring; Dina Elvia Rani
METANA Vol 14, No 1 (2018): Juni 2018
Publisher : Universitas Diponegoro

Sabun cuci piring merupakan salah satu kebutuhan dalam rumah tangga yang berfungsi sebagai penghilang kotoran dan lemak pada peralatan makan dan masak. Konsumsi sabun cuci piring yang terus menerus setiap harinya, menyebabkan kebutuhan pengadaan sabun dengan biaya yang tidak sedikit, terlebih pada ibu-ibu kelompok pembuat jajan pasar sederhana di RT 4 RW 7 Kelurahan Pedalangan Kecamatan Banyumanik. Tujuan dilaksanakannya pengabdian kepada masyarakat ini adalah untuk memberi pengetahuan dan pelatihan kepada ibu-ibu RT 4, RW 7, Kelurahan Pedalangan, Kecamatan Banyumanik tentang pembuatan sabun cuci piring cair guna mengurangi pengeluaran masyarakat terhadap pembelian sabun sekaligus untuk menciptakan peluang usaha baru. Dalam kegiatan pengabdian, akan dijelaskan bahan baku, prosedur kerja dan perhitungan biaya produksi pembuatan sabun cuci piring. Dari kegiatan yang dilakukan, mitra dapat menghasilkan luaran produk barang berupa sabun cuci piring cair sesuai dengan metode yang diberikan dalam pelatihan. Production of Dish Washer Soap as Efforts to Increase Effectiveness And Entrepreneurial Opportunity Dishwashing liquid is one of household stuff that used for removing grease and dirt from dishes. Using the dishwashing liquid continuously cause the need of its stuff at a high enough cost. The purpose of this activity is to provide knowledge and training to the making of dishwashing liquid soap in order to reduce cost as well as to create new business opportunity. In this activities, the raw materials and procedure of producing dishwashing liquid will explained, and the production costs of dishwashing manufacture will calculated. From the activities undertaken, can produce the dishwashing liquid soap in accordance with the methods given in the training.

OPTIMASI PRODUKSI PROTEIN SEL TUNGGAL DARI BAGASE TERHIDROLISA DENGAN FERMENTASI OLEH SACCAROMYCES CEREVICEAE Isti Pudjihastuti; Margaretha T S; Wahyuningsih Wahyuningsih; Edy Supriyo
METANA Vol 8, No 02 (2012): Desember 2012
Publisher : Universitas Diponegoro

The Came pulp (bagase) were main contained cellulose, through process delignification and hidrolized can be used to growting sacaromyces cereviceae yeast as fermented media, so can produced biomass microbial as known as single cell protein (SCP).Design experiment was random block with treatment nutrient added as long as fermented process. The measured parameter were protein contained in microbial biomass with Kehjdahl method. Optimum result were formed to 8 days fermentation and nutrient added (NH4)2SO4:1 gr,MgSO47H2O : 0,5 gr, molase (tetes) 20% : 2 ml to media 50 gr. Key word: bagase, Saccaromyces Cereviceae,SCP

Perancangan Pengering Berinsulasi Dengan Aplikasi Pengeringan Ikan Tengiri Wahyuningsih Wahyuningsih; Isti Pudjihastuti
METANA Vol 12, No 2 (2016): Desember 2016
Publisher : Universitas Diponegoro

Prinsip Dasar Rancang Bangun Alat Pengering, peubah-peubah yang harus diperhatikan antara lain Sifat – sifat bahan yang akan dikeringkan,karakteristik pengeringan dari bahan, aliran panas, kualitas produk, fasilitas sekeliling,kapasitas alat,Dari faktor – faktor tersebut diharapkan untuk mendapatkan alat pengering dengan kinerja yang berdayaguna dan berhasil guna. Tujuan dari penelitian ini adalah merancang dan membangun alat pengering ikan berinsulasi untuk memperbaiki proses pengeringan ikan tradisional,meningkatkan nilai gizi, hygiene dan keamanan pangan dan mengaplikasikan alat pengering pada pengeringan ikan teri (Stolephorus heterolobua), serta mengetahui kwantitas asam lemak omega-3. Alat pengering hasil rancang bangun, ukuran 1 m x 1,2m x1,75 m, ukuran kolektor 1,2 m x 0,6 m, dapat dimanfaatkan untuk mengeringkan ikan teri (Stolephorus heterolobua) secara higienis dan mempertahankan kualitas produk Lama pengeringan 72 jam memberikan hasil ikan kering yang memenuhi standar kadar air , untuk pemanas surya kadar air 2,45 %, untuk pemanas buatan kadar air 2,65% Produk yang disukai adalah pengeringan dengan kadar garam 6% Pada produk yang dapat diterima memberikan nilai EPA 0,5972 gr/100 gr, dan DHA 0,4032 gr/100 gr, nilai TBA 1,21 mgr/kg, TVB : 7,04mgr N/100gr,TMA : 5,11 % mgr N. Design of Insulated Dryers Application for Tengiri Fish Dryer Basic Principles of Design Tools dryer, variables that must be considered among other properties - properties of the material to be dried, the characteristic drying of the material, heat flow, quality of products, the facilities around, the capacity of the tool, From factors - these factors are expected to get drier the performance of a very powerful and effective. The aim of this study was to design and build insulated fish dryers to improve the traditional fish drying process, enhance nutritional value, food safety and hygiene and to apply drier at drying anchovy (Stolephorus heterolobua), as well as to know the quantity of omega-3 fatty acids. Drier the result of design, size 1m x 1.2m x1,75 m, collector size of 1.2 mx 0.6 m, can be used for drying fish (Stolephorus heterolobua) hygienically and maintain the quality of products Lama drying 72 hours results dried fish that meets dtandar water content, for solar heating water content of 2.45%, for artificial heating water content of 2.65% preferred product is dried with saline 6% On acceptable products value EPA 0.5972 gr / 100 g and 0.4032 g DHA / 100 g, the value of TBA 1.21 mgr / kg, TVB: 7,04mgr N / 100gr, TMA: 5.11% N mgr

TEKNOLOGI PRODUKSI PUPUK ORGANIK CAIR DARI LIMBAH SAMPAH RUMAH TANGGA DI KELURAHAN LEMPONGSARI, KODYA SEMARANG DENGAN KOMPOSER EM-4 Wahyuningsih Wahyuningsih; Edy Supriyo
METANA Vol 9, No 01 (2013): Juli 2013
Publisher : Universitas Diponegoro

Abstract In Semarang limited landfill waste,new problems.If problems not handled and managed properly,an increase of waste that occur each year could shorten justify the air.In addition,the waste can the quality of natural resources,causing floods,and cause some diseased. Research to be conducted has several specific objectives,as follomposer EM-4.Minimizing the volume of waste in Semarang environment develop a network tool composter for organic fertilizer production of large-scale environmental and examine the addition of decomposers EM-4 the formation of humus to improve the productivity of organic fertilizer in environmentally friendly, operating condition optimization weathering process household waste using waste decomposer EM-4. Composter network of productivity environmentally friendly is expected to replace the function of inorganic fertilizers Research results:the best is on addition EM-4 8 ppm,with the following results:value ratio C/N 14,77;P2O5 1,24%;K2O 0,42% SNI: ratio C/N 10-20 ;0,1% min P2O5 ;0,2%min K2O Keyword: EM-4 composer,liquid compost organic,organic waste

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