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The Distribution of Microalgae in a Stabilization Pond System of a Domestic Wastewater Treatment Plant in a Tropical Environment (Case Study: Bojongsoang Wastewater Treatment Plant) Ariesyady, Herto Dwi; Fadilah, Rifka; Kurniasih, Kurniasih; Sulaeman, Aminudin; Kardena, Edwan
Journal of Engineering and Technological Sciences Vol 48, No 1 (2016)
Publisher : ITB Journal Publisher, LPPM ITB

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (352.306 KB) | DOI: 10.5614/j.eng.technol.sci.2016.48.1.7

Abstract

The Bojongsoang Wastewater Treatment Plant (WWTP) serves to treat domestic wastewater originating from Bandung City, West Java, Indonesia. An abundant amount of nutrients as a result of waste decomposition increases the number of microalgae populations present in the pond of the wastewater treatment plant, thereby causing a population explosion of microalgae, also called algal blooming. In a stabilization pond system, the presence of algal blooming is not desirable because it can decrease wastewater treatment performance. More knowledge about the relationship between the nutrients concentration and algae blooming conditions, such as microalgae diversity, is needed to control and maintain the performance of the wastewater treatment plant. Therefore this study was conducted, in order to reveal the diversity of microalgae in the stabilization pond system and its relationship with the water characteristics of the comprising ponds. The results showed that the water quality in the stabilization pond system of Bojongsoang WWTP supported rapid growth of microalgae, where most rapid microbial growth occurred in the anaerobic pond. The microalgae diversity in the stabilization ponds was very high, with various morphologies, probably affiliated with blue-green algae, green algae, cryptophytes, dinoflagellates and diatoms. This study has successfully produced information on microalgae diversity and abundance profiles in a stabilization pond system.
PERBEDAAN HASIL BELAJAR GEOGRAFI ANTARA MEDIA POWER POINT DAN MEDIA CHART PADA MATERI HIDROSFER KELAS X SEMESTER GENAP TAHUN AJARAN 2012/2013 SMA NEGERI 1 BANJARHARJO, BREBES Kurniasih, Kurniasih
Edu Geography Vol 3 No 4 (2015)
Publisher : Edu Geography

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Abstract

Penelitian bertujuan: 1) Untuk mengetahui hasil belajar geografi materi hidrosfer yang pembelajarannya menggunakan media power point 2) Untuk mengetahui hasil belajar geografi materi hidrosfer yang pembelajarannya menggunakan media chart 3) Untuk mengetahui perbedaan hasil belajar geografi materi hidrosfer antara pembelajaran yang menggunakan media power point dengan media chart. Populasi penelitian yaitu siswa kelas X SMA Negeri 1 Banjarharjo. Teknik pengambilan sampel menggunakan purposive random sampling. Variabel penelitian: 1) Hasil belajar siswa materi hidrosfer menggunakan media pembelajaran power point. 2) Hasil belajar siswa materi hidrosfer menggunakan media pembelajaran chart. Teknik analisis data yang digunakan yaitu menggunakan analisis deskriptif persentatif dan uji statistik uji-t. Hasil penelitian menunjukkan: 1) Hasil belajar menggunakan media power point nilai rata-rata kognitif 80,65, sedangkan nilai rata- rata afektif pertemuan I 82,6, pertemuan II 85,1, dan pertemuan III 88,2. 2) Hasil belajar menggunakan media chart nilai rata-rata kognitif 72,60, sedangkan rata-rata nilai afektif pertemuan I 71,5, pertemuan II 76,9, dan pertemuan III 83,1. 3) Uji perbedaan diketahui bahwa t_hitung 4,095  dan  ttabel 1,67, taraf signifikasi 5% dan dk= 33 + 33 – 2 = 64, karena thitung > ttabel sehingga Ho ditolak dan Ha diterima. Research aims: 1) To assess the learning outcomes of the hydrosphere geography learning materials using medium power point  2) To know the result of that study geography hydrosphere learning material using a media chart 3) To determine differences in learning outcomes between the hydrosphere geography teaching materials using the media power point with media charts. The study population are students of class X SMA Negeri 1 Banjarharjo, Purposive sampling technique using random sampling. Research variables: 1) Student learning outcomes hydrosphere material using instructional media power point. 2) Student learning outcomes hydrosphere material using instructional media charts. Data analysis techniques used are persentatif using descriptive analysis and t-test statistical test. The results showed: 1) The results of learning to use the media power point average cognitive score of 80,65, while the average value of 82,6 affective first meeting, the meeting II 85,1, and meeting III 88,2. 2) The result of learning to use the media charts the average value of cognitive 72,60, while the average value of 71,5 affective first meeting, the meeting II 76,9, and meeting III 83,1. 3) Test the difference is know that t-hitung 4.095 and t-tabel 1.67, 5% significance level and df = 33 + 33 – 2 = 64, because of t-hitung > t-table so that Ho is rejected and Ha sccepted.
Lactic Acid Bacteria Isolated from the Gastro-Intestinal Tract of Chicken: Potential Use as Probiotic Harimurti, S; Nasroedin, ES; Nasroedin, Nasroedin; Kurniasih, Kurniasih
ANIMAL PRODUCTION Vol 9, No 2 (2007): May
Publisher : Universitas Jenderal Soedirman, Faculty of Animal Science, Purwokerto-Indonesia

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Abstract

Lactic acid bacteria have been suggested to have several beneficial effects on human and animals.  These bacteria, indigenous to the gastro-intestinal tract, are important in regulating the balance among the desirable and undesirable intestinal microflora and in controlling enteric pathogenic infection in the host.  Objectives of this research are to obtain lactic acid bacteria isolates from gastro-intestinal tract of chicken and to screen their ability as a probiotic agent i.e., their antagonistic against pathogenic bacteria, their survival at low pH and high concentration of bile salt.  In this research, 74 samples used as sources of bacteria, and among them only 11 samples could be isolated as lactic acid bacteria with the total number of isolates of 61.  Based on the preliminary screening i.e., their antagonistic factor against pathogenic bacteria, 20 isolates was further studied.   Based on the identification scheme, these isolates belong to three species, i.e., Lactobacillus murinus, Pediococcus acidilactici, and Streptococcus thermophilus. The result showed that most isolates grow well in the media with the initial pH of 5.5, but their growth were retarded when the initial pH 3.5.  Only one isolate Streptococcus thermophilus Kp-2 showed its growth at initial pH of 3.5.   All isolates did not show any growth at initial pH 2.5, though their viability still high. The result based on the isolates resistance to bile salt showed that most isolates could grow at media with 0.20% of bile salt.  Their growth was inhibited with the increasing bile salt concentration.  However, few isolates could grow well at media with 1% of bile salt. Based on their characteristics three isolates i.e., Lactobacillus murinus Ar-3, Streptococcus thermophilus Kp-2, and Pediococcus acidilactici Kd-6 were selected as probiotic agents for the continuing research. i.e. production of biomass and its application to chicken production. (Animal Production 9(2): 82-91 (2007) Key Words : Lactic acid bacteria, gastro- intestinal tract of chicken, probiotic agents
Identification of Excretory – Secretory Sporozoite Antigens of Eimeria tenella with Monoclonal Antibody Prastowo, J; Nurcahyo, W; Kurniasih, Kurniasih; Wasito, R
ANIMAL PRODUCTION Vol 7, No 2 (2005): May
Publisher : Universitas Jenderal Soedirman, Faculty of Animal Science, Purwokerto-Indonesia

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Abstract

The research done specifically to identified the. Eimeria tenella excretory-secretory sporozoit antigen by using the monoclonal antibody reaction instead. Eimeria tenella excretory-secretory sporozoit antigen. The 107 sporozoits were obtained from existation of 5x107 oocysts with 25 ml tripsin and sodium taurocholat. Excretion - secretion antigen were preparated by freezing and thawing and then be isolated with ice bath 20 second, 20 meter of amplitude as the further treatment.  Determinating the excretion -secretion sporozoit protein by using BCA-test. While the visualization sporozoit protein antigen by SDS-PAGE. Monoclonal antibody were produced through hybridization between B BALB/c limphosit immunized with Eimeria tenella excretion-secretion sporozoite antigen and myeloma cells. The monoclonal antibody identification  of excretion – secretion antigen were done through western blott. The visualization of protein molecule weight of Eimeria tenella sporozoit were resulted 20 protein fraction, included 14, 16, 17, 18, 20, 24, 26, 32, 34, 36, 39, 43, 47, 56, 65, 67, 76, 87, 94, and 96 KDa. There were 12 kinds of monoclonal antibody could be produced from these proteins. While the 5 hybridoma immunoblotting resulted specific reaction by the appearances of reaction ribbon obviously, there were MABset 1 which identified protein epitop with 14.7 KDa of molecule weight,  MAbset 2 which identified protein epitop with 43 KDa of molecule weight, MAbset 3 which identified protein epitop with 42 KDa of molecule weight, MAbset 4 which identified protein epitop with 47 KDa of molecule weight and MAbset 5 which identified protein epitop with 90 KDa of molecule weight. As the conclusion, five of excretion-secretion sporozoit antigen were proper immunogen to stimulate the hospest immunity. (Animal Production 7(2): 95-100 (2005) Key Words: Sporozoit, Antigent, Eimeria tenella, Coccidiosis
Animated Stories In The Teaching Of Writing Rahmawati, Henny; Kurniasih, Kurniasih
JU-ke: Jurnal Ketahanan Pangan Vol 1, No 2 (2017): JU-Ke
Publisher : Universitas Islam Malang

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This study is aimed at investigating the benefits of animated stories in the teaching of writing as suggested by Cahyono, Hidayati, & Zen (2014). This study is, say, a literature review study in which the researcher reviews the material related to the issue and observes the students as well to strengthen the validity of this study. The data obtained from both literature and observation are then analyzed qualitatively showing that the animated stories are not only interesting for the students in writing class but also enable them to learn based on their learning style
CRITICAL READING SKILL AND ITS IMPLICATION TO SPEAKING ABILITY IN MULTICULTURAL CLASSROOM Widowati, Diah Retno; Kurniasih, Kurniasih
Celtic: A Journal of Culture, English Language Teaching, Literature and Linguistics Vol 5, No 2 (2018): December 2018
Publisher : University of Muhammadiyah Malang

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (416.499 KB) | DOI: 10.22219/celtic.v5i2.7615

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People need good background knowledge before they speak. The purpose of the present study was to discuss the implication of students’ critical reading skill to their English speaking performance in classroom activity. Researchers also would like to discuss the effect of multicultural atmosphere to students’ speaking skill. This study employed descriptive qualitative design to explain students’ speaking ability after they read. Therefore, students’ speaking ability could be mapped out after being stimulated by critical reading. The major finding of this study were the fact that reading and analyzing the texts was important for students before they speak. Gaining previous information before students speak made them feel more comfortable and be able to deliver or speak up their ideas better. Besides, the multicultural background of the students were not significantly affect their speaking ability. Yet, some students felt difficult in pronouncing ‘f’ in ‘of’, ‘v’ in very’, and ‘o’ in open’ correctly. This was because of their pronunciation habit in their homeland.
POTENSI DAUN BAMBU SEBAGAI AGEN ANTHELMETIKA PADA TERNAK KAMBING ( Bamboo Leaves Potency as anthelmintic Agent on Goat) Widiarso, Widiarso, B. P.; Nurcahyo, Wisnu; Prastowo, Joko; Kurniasih, Kurniasih
Jurnal Pengembangan Penyuluhan Pertanian Vol 14, No 1 (2017): Jurnal Pengembangan Penyuluhan Peternakan
Publisher : UPPM Politekik Pembangunan Pertanian Yogyakarta Magelang (Polbangtan Yoma)

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Daun bambu telah digunakan secara luas sebagai pakan alternatif pakan ternak ruminansia, namun dalam penggunaannya di lapangan, belum banyak dikaji manfaat lain selain sebagai sumber pakan. Kandungan tannin dalam daun bambu memberikan potensidaun bambu sebagai agen antelmetika. Selain mengandung kandungan nutrisi daun bambu: berat kering 91,27%; protein kasar 4,24%; lemak kasar 8,11%; serat kasar 27,2%; total digesti nutrien 36,42% .Daun bambu (Dendrocalamus strictus) setiap 100 mg mengandung Protein Kasar 15,09; Serat Kasar, 23,15; Lemak Kasar 1,43; Abu 18,03; Fosfor 170; Kalsium, 1550 mg (Attayaya, 2009). Tanin dalam daun bambu apus (Gigantochloa apus) tua 8,81% b/b, tanin dalam daun bambu petung (Dendrocalamus asper) tua 4,84% b/b, dan tanin dalam daun bambu legi (Gigantochloa atter ) tua 3,19% b/b. Hasil pengujiankandungan tanin di atas dapat menunjukkan bahwa daun bambu mepunyai potensi sebagai anthelmetika melawan cacing gastrointestinal. Tanin yang terdapat pada daun bambu adalah tanin terkondensasi. Tanin terkondensasi efektif melawan parasit GI. Efek tanin terkondensasi melawan parasit GI dilakukan baik secara langsung, yaitu melalui interakasi TK-nematoda, mempengaruhi penetasan dan mempengaruhi pertumbuhan larva infektif, maupun secara tidak langsung, yaitu dengan cara mengikat protein tumbuhan di dalamrumen sehingga mencegah degradasi mikrobial sehingga meningkatkan aliran protein ke duodenum yang pada akhirnya akan meningkatkan imunitas hospesKata Kunci: Antelmetika, Daun bambu, Kambing
Phylogenetic Tree dari Empat Isolat Edwardsiella Tarda di Indonesia Narwiyani, Siti; Kurniasih, Kurniasih
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 16, No 2 (2011): June 2011
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (171.155 KB) | DOI: 10.24002/biota.v16i2.118

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Penelitian ini bertujuan mengetahui adanya kemungkinan subspecies Edwardsiella tarda secara molekuler. Edwardsiella tarda dapat diisolasi dari ikan Tilapia dan air yang tercemar dan diidentifikasi secara PCR dengan amplifikasi gen hemolysin. Isolat E. tarda diperoleh dari ikan nila (Yogyakarta), lele (Semarang dan Jambi), kura-kura impor (Brazilia), ikan mas (Pontianak). Isolat atipikal E. tarda (ATCC) dari Singapura digunakan sebagai pembading 4 isolat E. tarda dari Indonesia. Semua isolat E. tarda diekstraksi, diamplifikasi rRNA pada SSU 16S dan disequencing. Hasil sekuensing allignment menggunakan program CLUSTAL W versi 1.8. Selanjutnya dianalisis dengan metode neighbour-joining dan metode maximum parsimony untuk menghasilkan pohon phylogenetik (Saitou dan Nei, 1987). Phylogenetic tree menunjukkan bahwa 3 isolat E. tarda dari ikan merupakan strain yang sama dibanding E. tarda dari kura- kura Brazil dan isolat ATCC yang berasal dari manusia.
EVALUASI MATERIAL RADIANT TUBE HEATER DI UNIT REFINERY MENGGUNAKAN METODE TEKNIK REPLIKA DAN KEKERASAN Syahril, Muhammad; Kurniasih, Kurniasih
Majalah Ilmiah Pengkajian Industri Vol 13, No 3 (2019): Majalah Ilmiah Pengkajian Industri
Publisher : BPPT

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (504.046 KB) | DOI: 10.29122/mipi.v13i3.3621

Abstract

Tulisan ini dibahas tentang evaluasi materialradianttube refinery heateryang telah beroperasi selama ± 15 tahun dengan metode teknik replika dan uji kekerasan. Secara visual, tube yang merupakan spesifikasi dari material 1¼Cr-½Mo-Si telah terdegradasi akibat terekspos pada temperatur tinggi dalam waktu yang lama. Evaluasi dilakukan secara analitis untuk mengetahui kondisi aktual tube dan menjamin kehandalan tube terhadap beban operasi yang akan datang.Studi ini dilakukan dengan melakukan pemeriksaan visual, struktur mikro dengan teknik replika dan uji kekerasan material dengan alat uji kekerasan portable. Pengamatan pada radianttubemenunjukkan bahwa struktur mikro material tube berupa fasa ferit dan perlit yang mulai terspheroidisasi,batas butir masih cukup jelas, nilai kekerasan minimum sebesar 127 HB (kekerasan brinnell) yang identik dengan nilai kekuatan tarik sebesar 438 MPa.Analisis kekuatan material dari nilai kekerasan mengindikasikan bahwa material tube masih dalam kriteria spesifikasi standar material 1¼Cr-½Mo-Si, dimana kekuatanstandarnya adalah minimum 415 MPa. Berasarkan analisis struktur mikro dan nilai kekerasan dapat disimpulkan bahwa material tube heater masih layak dan handal untuk difungsikan dalam operasi sesuai kondisi desain. 
KELANGSUNGAN HIDUP IKAN MAS (Cyprinus carpio L) YANG DIBERI PAKAN EKSTRAK BAWANG PUTIH (Allium sativum) DAN DI INFEKSI Aeromonas hydrophila Lukistyowati, Iesje; Kurniasih, Kurniasih
Jurnal Perikanan dan Kelautan Vol 16, No 02 (2011)
Publisher : Fakultas Perikanan dan Kelautan Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31258/jpk.16.02.%p

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The aim of a research were to investigate the effectiveness of garlic extractmixed with feed given 30 days in encharging common carps after challen with againstAeromonas hydrophila with density of 106 cell/ml. This research conducted by usingcomplete random design with dose addition of garlic extract to feed were controlpositive, control negative, P1 (2,5%), P2 (5%), and P3 (10%). In order to fine out theactive component of garlic extraction methode was adopted using 70% ethanol. Theextracted filtrates were separated using the spectrophotometrical chromatrographymethod (GC-MS) and thin-layered chromatography. Identification of the maincomponents found in the ethanol extract of garlic revealed 13 components but the onethat served as antimicrobial was C6H10S2 CAS disulphide di 2-propenyl(CAS)/(Diallyldisulfide) with and area of 16,95%. Beside the result of thin layeredchromatography showed that garlic extact contained sapononin which is harmful toaquatic animals. Percentage of survival rate P3 (10%) after infected after withAeromonas hydrophila was different compare witn control positive. The highsurvival shown on P3 (10%) concentration with 70%, P1 60%, control negative 60%and P2 30%. Fourteen days after injection the cut cause by Aeromonas hydrophilaclose and the fish recuperated as shown by their lively movement.