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THE IDENTIFICATION OF ANTIBACTERIAL COMPOUNDS IN CLOVE STEM EXTRACT (Syzygium aromaticum) AND ITS EFFECTIVENESS IN INHIBITING THE GROWTH OF Escherichia coli Yunita Diyah Safitri; Ana Amalia; Fatimah Fatimah; Afidatul Muadifah
JBIO: jurnal biosains (the journal of biosciences) Vol 8, No 1 (2022): JBIO: jurnal biosains (the journal of biosciences)
Publisher : Universitas Negeri Medan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24114/jbio.v8i1.28089

Abstract

Clove plants usage in daily life is very diverse, such as kitchen spices, mixtures of various drinks, and medicines. Clove is a spice plant that contains clove oil which is known as an antibacterial agent. The clove stem has a fairly high clove oil content, but this is not widely known by the public. Clove oil is known to have the ability as a fungicide, bactericide, insecticide, and nematicide. The purpose of this study is to identify antibacterial compounds and the effectiveness of clove stem extract on the growth of Escherichia coli. We used GCMS (Gas Chromatography Mass Spectrometry) analysis to identification antibacterial compounds. Antibacterial activity test using paper disc method by measuring the diameter of the inhibition zone formed from clove stem extract at doses of 30%, 50%, and 70% against Escherichia coli. The results of the study were analyzed using ANOVA with significance (p<0.05). The identification of the active compound showed 13 kinds of compounds were found with the dominant compound was alpha-pinene. The results of the antibacterial test showed the antibacterial activity of clove stem extract at doses of 30%, 50% and 70% with inhibition zones formed 12 mm, 12.7 mm, and 14.1 mm respectively. 
Studi aktivitas ekstrak etanol dan sediaan gel daun melinjo (Gnetum gnemon L) sebagai antibakteri terhadap Staphylococcus Aureus Indra Lasmana Tarigan; Afidatul Muadifah; Helda Wika Amini; Tri Kurnia Astutik
CHEMPUBLISH JOURNAL Vol. 4 No. 2 (2019): Chempublish Journal
Publisher : Universitas Jambi, Fakultas Sains dan Teknologi, Program Studi Kimia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22437/chp.v4i2.7631

Abstract

Melinjo (Genus Gnetii gnemonii Folium) merupakan spesies tanaman yang berasal dari semenanjung Malaysia dan Indonesia, diketahui memiliki senyawa bioaktif yang dapat dimanfaatkan dibidang kesehatan. Tujuan penelitian ini ini adalah untuk menganalisis profil senyawa bioaktif daun melinjo (Gnetii gnemonii Folium) yang memiliki fungsi sebagai antimikroba dengan menghambat pertumbuhan bakteri Staphylococcus aureus serta memformulasi gel dari ekstrak daun melinjo. Ekstrak daun melinjo dibuat dengan metode maserasi menggunakan pelarut etanol 70%, 3 hari. Analisis profil senyawa bioaktif daun melinjo menggunakan skrining fitokimia dan pengujian aktivitas antibakteri dilakukan menggunakan metode difusi cakram Kirby-Bauer terhadap Staphylococcus aureus. Konsentrasi ekstrak etanol daun melinjo yang digunakan interval konsentrasi 50%-80%. Ekstrak dengan konsentrasi optimum diformulasikan dalam bentuk gel dan dilakukan evaluasi mutu fisik sediaan gel meliputi organoleptis, uji homogenitas, uji pH, uji daya lekat dan uji daya sebar selama 28 hari. Hasil analisis menunjukkan senyawa bioaktif daun melinjo meliputi Tanin, Saponin, Alkaloid, Flavonoid, dan Triterpenoid. Ekstrak 80% daun melinjo memberikan efek antibakteri dengan zona hambat terbesar 13,08mm. Gel dari ekstrak daun melinjo 80% memberikan efek antibakteri dengan kategori kuat terhadap bakteri Staphylococcus aureus dengan diameter zona hambat sebesar 16,91mm.
A Natural Antiseptic Alternative in Hand Sanitizer Gel : A Combination of Miana and Kemuning Leaves Extract Afidatul Muadifah; Dara Pranidya Tilarso; Ary Kristijono; Khoirul Ngibad; Nabila Putri Salsabila
JI-KES (Jurnal Ilmu Kesehatan) Vol 5, No 2 (2022): JI-KES (Jurnal Ilmu Kesehatan)
Publisher : STIKes Hafshawaty Pesantren Zainul Hasan Probolinggo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33006/ji-kes.v5i2.266

Abstract

Abstrak Penggunaan gel hand sanitizer saat ini menjadi jalan keluar untuk menjaga kesehatan dan kebersihan tangan agar lebih praktis dan mudah dibawa. Penelitian ini bertujuan untuk menghasilkan antiseptik alami dari kombinasi ekstrak daun Miana dan Kemuning terhadap Staphylococcus aureus dan Escherichia coli dalam bentuk gel. Gel hand sanitizer dibuat dari kombinasi ekstrak daun miana dan kemuning yang menunjukkan daya hambat optimum terhadap bakteri Staphylococcus aureus dan Escherichia coli. Kombinasi ekstrak daun Miana dan Kemuning dibuat dalam 3 variasi konsentrasi (K1 = 5%: 15%), (K2 = 10%: 10%), dan (K3 = 15%: 5%). Formulasi gel hand sanitizer diuji kemampuannya sebagai antiseptik dan uji stabilitas fisik pada hari ke 0, hari ke 7, hari ke 14, hari ke 21, dan hari ke 28. Konsentrasi optimum dari kombinasi ekstrak daun miana dan kemuning sebagai antiseptik adalah pada kombinasi K3 = 15%: 5%. Kombinasi antiseptik alami K3 (NAK3) menunjukkan daya hambat sebesar 7,33 mm (terhadap Staphylococcus aureus) dan 09,00 mm (terhadap Escherichia coli) yang termasuk dalam kategori sedang. NAK3 memenuhi persyaratan untuk uji organoleptik, homogenitas, pH, kemampuan menyebar, dan adhesi, serta stabil selama penyimpanan..  Kata kunci: antiseptik, miana, kemuning, gel, hand sanitizer Abstract The use of hand sanitizer gel is currently a more practical and portable way to maintain health and hand hygiene. This study aims to generate a natural antiseptic from a combination of Miana and Kemuning leaves extract toward Staphylococcus aureus and Escherichia coli in gel formulation. The hand sanitizer gel is made from a combination of Miana and Kemuning leaves extract which shows optimum inhibition against Staphylococcus aureus and Escherichia coli bacteria. The combination of Miana and Kemuning leaves extracts was made in 3 variations of concentration (K1 = 5%: 15%), (K2 = 10%: 10%), and (K3 = 15%: 5%). The hand sanitizer gel formulation tested its ability as an antiseptic and on physical stability test on day 0, day 7, day 14, day 21, and day 28. The optimum concentration of the combination of Miana and Kemuning leaves extracts as an antiseptic is at a combination of K3 = 15%: 5%. The combination K3 Natural Antiseptic (NAK3) showed an inhibitory power of 7.33 mm (against Staphylococcus aureus) and 09.00 mm (against Escherichia coli) which was included in the moderate category. The NAK3 meets the requirements for organoleptic tests, homogeneity, pH, spreadability, and adhesion, and is stable during storage. Keywords: antiseptic, miana, kemuning, gel, hand sanitizer
Anti-Cancer Activity Testing of Cumin (Plectranthus Amboinicus) Ethanol Extract Against Artemia Salina Leach by Using Brine Shrimp Lethality Test (BSLT) Method ika Rachutami; Rahma Diyan Martha; Afidatul Muadifah; Algafari Bakti Manggara
Walisongo Journal of Chemistry Vol 5, No 1 (2022): Walisongo Journal of Chemistry
Publisher : Department of Chemistry Faculty of Science and Technology Walisongo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21580/wjc.v5i1.9086

Abstract

The purpose of this study was to determine the anticancer activity of cumin leaf extract (Plectranthus amboinicus), which was applied using the Brine Shrimp Lethality Test (BSLT) method to determine the level of acute toxicity (LC50) of the extract against Artemia salina LEACH larvae. Cumin leaf samples were extracted using the maceration method with 96% ethanol. The cumin leaf extract was then tested qualitatively and quantitatively. Toxicity testing used concentrations of 500 ppm, 1000 ppm, 1500 ppm, 2000 ppm, and 2500 ppm, which were given to Artemia salina LEACH larvae for 24 hours. Calculate the LC50 value in this study using probit analysis. The results of qualitative and quantitative testing showed the presence of saponins by 5.20%, tannins by 8.21%, flavonoids by 23.93%, and alkaloids by 4.37%. The results of the acute toxicity test of the extract using the BSLT method showed that there was anticancer activity with the acquisition of LC50 of 1000 ppm, which was 697.99 ppm.
Program Pendampingan Cara Pemilihan Produk Skincare Aman Untuk Kulit Sehat Bagi Ibu-Ibu Paruh Baya Di Desa Krebet Kecamatan Srengat Afidatul Muadifah; Khoirul Ngibad
Jurnal ABDINUS : Jurnal Pengabdian Nusantara Vol 5 No 1 (2021): Volume 5 Nomor 1 Tahun 2021
Publisher : Universitas Nusantara PGRI Kediri

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29407/ja.v5i1.15311

Abstract

The purpose of this community service is to increase knowledge on the skin health of middle-aged mothers in Krebet Village, Srengat District through a mentoring program for the use of safe skincare. The methods used are (1) observation and interviews; (2) Learning; (3) Assistance; and (4) Evaluation. Results: Middle-aged mothers who have used various types of skincare products obtained from doctor's clinics and cosmetic stores are 24 people. The results of learning on middle-aged mothers in Krebet Village, Srengat District of the qualitative test method and the dangers of mercury and hydroquinone in skincare products show a good value. with a percentage increase in knowledge of 67%. The results of assistance to middle-aged mothers in Krebet Village, Srengat District in stopping the use of positive skincare containing mercury and hydroquinone through replacement with skincare products that are safe for healthy skin shows good results with a 75% increase in skills so that the quality of health is better maintained.
Pemberdayaan Masyarakat Desa Srengat Dalam Pembuatan Antiseptik Alami Dari Ekstrak Daun Miana Afidatul Muadifah; Khoirul Ngibad; Nabila Puteri Salsabela
Jurnal Kreativitas Pengabdian Kepada Masyarakat (PKM) Vol 5, No 2 (2022): Volume 5 No 2 Februari 2022
Publisher : Universitas Malahayati Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33024/jkpm.v5i2.4726

Abstract

ABSTRAK Sulit diterapkan protokol kesehatan dalam upaya pencegahan covid-19 di Desa Srengat, karena memberikan pendidikan kepada masyarakat desa dengan latar belakang yang mayoritas adalah petani dan peternak (bukan akademisi) adalah cukup sulit. Mereka lebih mengedepankan kepercayaan dalam bertindak daripada menggunakan ilmu pengetahuan. Pada sisi lain, ketersediaan tempat mencuci tangan juga kurang memadai  pada mayoritas rumah, sehingga membuat masyarakat semakin tidak terampil dalam mencuci tangan setelah keluar rumah ataupun ketika bertamu. Pengetahuan akan penggunaan antiseptik seperti handsanitizer masih rendah, dan sebagian masyarakat yang mengetahui lebih memperhitungkan biaya jika harus membelinya, sehingga membuat ketersediaan handsanitizer dirumah-rumah warga sangat terbatas padahal banyak masyarakat yang sudah menanam tanaman Miana yang berpotensi sebagai antiseptik. Tujuan dan metode: Meningkatkan pengetahuan masyarakat Desa Srengat tentang covid-19 melalui penyuluhan, pemberdayaan Jam’iyah Yasin Desa Srengat dalam peningkatan jumlah tanaman miana melalui gerakan menanam, dan difusi ipteks serta pelatihan kepada Jam’iyah Yasin Desa Srengat dalam pembuatan produk gel handsanitizer dari ekstrak daun miana sebagai alternatif antiseptik alami dan diberikan secara gratis untuk masyarakat Desa Srengat. Hasil : Program penyuluhan berjalan dengan baik dengan adanya peningkatan pengetahuan masyarakat sebesar 60%, tanaman Miana mengalami peningkatan hasil panen sebesar 62,5% pada bulan ke-3, dan ketersediaan handsanitizer di Desa Srengat sudah cukup memadai (tersedia pada 40 Kartu Keluarga) sehingga dapat digunakan sebagai alternatif antiseptik alami pengganti cuci tangan. Kata Kunci: Antiseptik Alami; Miana; Hand sanitizer; Desa Srengat  ABSTRACT It is difficult to apply health protocols in an effort to prevent covid-19 in Srengat Village, because providing education to village communities with backgrounds whose majority are farmers and ranchers (not academics) is quite difficult. They prioritize trust in action rather than using knowledge. On the other hand, the availability of a place to wash hands is also inadequate in the majority of homes, thus making people increasingly unskilled in washing their hands after leaving the house or when visiting. Knowledge of the use of antiseptics such as hand sanitizers is still low, and some people who know are more aware of the costs if they have to buy them, thus making the availability of hand sanitizers in people's homes very limited even though many people have planted Miana plants which have the potential as antiseptics. Objectives and methods: Increasing the knowledge of the Srengat Village community about covid-19 through counseling, empowerment of Jam'iyah Yasin in Srengat Village in increasing the number of miana plants through planting movements, and the diffusion of science and technology as well as training for Jam'iyah Yasin in Srengat Village in making hand sanitizer gel products from miana leaf extract as an alternative to natural antiseptic and given free of charge to the people of Srengat Village. Results: The extension program went well with an increase in community knowledge of 60%, Miana plants experienced an increase in yields of 62.5% in the 3rd month, and the availability of hand sanitizer in Srengat Village was sufficient (available on 40 Family Cards) so that can be used as an alternative to natural antiseptics instead of hand washing. Keywords: Natural Antiseptic, Miana, Hand sanitizer, Srengat Village
Validasi Metode Penetapan Kadar Boraks pada Kerupuk Puli Menggunakan Spektrofotometer UV-Vis: Validation of Methods of Borax Concentrations Determination in Puli Crackers Using a UV-Vis Spectrophotometer Oppie Anngela; Afidatul Muadifah; Dhanang Prawira Nugraha
Jurnal Sains dan Kesehatan Vol. 3 No. 4 (2021): Jurnal Sains dan Kesehatan
Publisher : Fakultas Farmasi, Universitas Mulawarman, Samarinda, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (271.998 KB) | DOI: 10.25026/jsk.v3i4.258

Abstract

Rice cracker or known as Krupuk puli is the kind of deep-fried crackers in Indonesia that made of rice seasoned with spices and flavor enhancer. Most Rice cracker sellers that peddle their products in the Ngunut traditional market, so that the sellers have to make or prepare the rice cracker as well as possible to make the cracker can be sold. In this case, most manufacturers add hazardous materials (borax) in the process of making rice crackers. The purpose of this research is to validate the method and to know the borax level of rice crackers sold in the Ngunut traditional market by using the UV-Vis spectrophotometry method. Optimization of borax wavelength in the wavelength range of 500-600 nm. The prepared samples are analyzed using UV-Vis spectrophotometry at the maximum wavelength that is 506 nm. The next step, is, validation methods, including linieritas test, accuracy test, precision test, and LOD test & LOQ. The result of this study obtained the optimum wavelength is 506 nm. Based on the validation methods that have been done, obtained the results of inference linearity in the concentration range of 5ppm; 20ppm; 35ppm; 50ppm; 65ppm with a physical value of R2 correlation of 0.993,%recovery by 96.5%, the precision value obtained by 0.375%, and the LOD value of 48.565 ppm amounting and a Loq value of 161.381 ppm. The borax level in sample A amounted to 1,380 ± 1.824 ppm, sample B of 852.1 ± 2.367 ppm, sample C of 1,373 ± 1.824 ppm, and sample D of 185.9 ± 1.788 ppm.
Uji Aktivitas Antibakteri Sediaan Gel Hand Sanitizer Ekstrak Kulit Buah Jengkol (Archidendron pauciflorum (Benth.) Nielsen) terhadap Bakteri Escherichia coli: Antibacterial Activity Test for Gel Hand Sanitizer of Jengkol Rind (Archidendron pauciflorum (Benth.) Nielsen) Extract against Escherichia coli Bacteria Rizka Ahyar Hidayati; Ary Kristijono; Afidatul Muadifah
Jurnal Sains dan Kesehatan Vol. 3 No. 2 (2021): Jurnal Sains dan Kesehatan
Publisher : Fakultas Farmasi, Universitas Mulawarman, Samarinda, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (273.108 KB) | DOI: 10.25026/jsk.v3i2.259

Abstract

Escherichia coli is a bacteria that can cause diarrhea. The emergence of diarrhea is caused by a lack of public awareness in maintaining cleanliness, including hand hygiene. Along with the times, people prefer to use hand sanitizers that are more practical, easy to carry, and easy to use. However, most hand sanitizer preparations contain alcohol which has the potential to cause dryness and irritation if used continuously. Therefore, jengkol rind is used which contains flavonoid compounds, saponins, tannins, and anthraquinones which have antibacterial properties against Escherichia coli. The purpose of this study was to determine the antibacterial activity of the hand sanitizer gel of jengkol fruit peel extract against Escherichia coli using the disc diffusion method. The research method used was experimental. The jengkol rind samples were extracted using the maceration method with 70% ethanol. The positive control used was a hand sanitizer with chloroxylenol content and the negative control was a gel base without extract. Jengkol rind extract is made into preparations in concentrations of 5%, 10%, and 15%. The preparation was tested for physical stability for 28 days including organoleptic, pH, homogeneity, dispersibility, adhesion, protective power, and drying time. The results showed that the preparations with a concentration of 5%, 10%, and 15% jengkol fruit peel extract had an average inhibition zone of 7.00 ± 0.8165 mm, 13.00 ± 0.8165 mm, and 17.25 ± 0.95743 mm. Of the three formulations, the formulation with a concentration of 10% is the most effective concentration because a small concentration already has a strong inhibitory power category. The hand sanitizer gel of jengkol fruit peel extract fulfills the requirements for organoleptic tests, homogeneity, pH, spreadability, adhesion, and protection power and is stable in the 28-day storage period, but does not meet the requirements for drying time.
Analisa Perbandingan Kadar Vitamin C Sediaan Kapsul Bubuk Bawang Putih (Allium sativum, L.) Menggunakan Metode Spektrofotometri UV-Vis: Comparative Analysis of Vitamin C Contents in the Garlic (Allium sativum, L.) Powder Capsules Using the UV-Vis Spectrophotometric Method Ema Krismawar Sari; Rahma Diyan Martha; Afidatul Muadifah
Jurnal Sains dan Kesehatan Vol. 3 No. 4 (2021): Jurnal Sains dan Kesehatan
Publisher : Fakultas Farmasi, Universitas Mulawarman, Samarinda, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (304.914 KB) | DOI: 10.25026/jsk.v3i4.260

Abstract

Abstrak Antioksidan dan penangkal radikal bebas yang dapat merusak sel yaitu fungsi dari vitamin C. Sumber Vitamin C adalah buah-buahan dan sayuran seperti bawang putih. Bawang putih dapat digunakan sebagai alternatif terapi oleh masyarakat dalam bentuk kapsul bubuk dalam berbagai merk. Penelitian ini bertujuan untuk mengetahui perbandingan kadar vitamin C pada lima merk sediaan kapsul bubuk bawang putih. Metode yang digunakan pada penelitian ini yaitu spektrofotometri UV-Vis dan validasi metode seperti uji linieritas, uji presisi, uji akurasi, dan uji LOD dan LOQ. Berdasarkan hasil penelitian didapat panjang gelombang Vitamin C yaitu 247nm dan diperoleh hasil linieritas dalam rentang konsentrasi 5ppm, 10ppm, 15ppm, 20ppm, 25ppm dengan nilai koefisien korelasi yaitu 0,966, limit deteksi 0,160ppm dan limit kuantitasi yaitu 0,365ppm. Akurasi dari metode ini ditentukan berdasarkan hasil perolehan kembali menggunakan metode spike standar, sedangkan presisi diukur dengan menghitung simpangan baku relatif. Kadar vitamin C dalam sampel 1 sebesar 1,755ppm, sampel 2 sebesar 1,444ppm, sampel 3 sebesar 1,231ppm, sampel 4 sebesar 1,134ppm, sampel 5 sebesar 0,853ppm. Hasil penelitian disimpulkan bahwa analisis dalam penetapan kadar vitamin C pada kapsul bubuk bawang putih dengan spektrofotometri UV-Vis terdapat perbedaan bermakna dari kadar vitamin C pada lima merek kapsul bubuk bawang putih yang menggunakan analisis statistika SPSS16 dengan metode Kruskal Wallis yaitu mendapatkan nilai p = 0,009.
Validasi Metode Penetapan Kadar Pengawet Natrium Benzoat pada Sari Kedelai di Beberapa Kecamatan di Kabupaten Tulungagung Menggunakan Spektrofotometer Uv-Vis Siti Awwalul Amanatur Rohmah; Afidatul Muadifah; Rahma Diyan Martha
Jurnal Sains dan Kesehatan Vol. 3 No. 2 (2021): Jurnal Sains dan Kesehatan
Publisher : Fakultas Farmasi, Universitas Mulawarman, Samarinda, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (693.319 KB) | DOI: 10.25026/jsk.v3i2.265

Abstract

Sodium benzoate is an organic preservative chemical compound which use is permitted if the amount is below the maximum threshold. This research aims to validate the UV-Vis Spectrophotometry method which will be used to determine sodium benzoate levels in soybean milk in 3 sub-districts in the Tulungagung Regency using the UV-Vis Spectrophotometer instruments. Before determining the content, the samples were analyzed qualitatively first using the acid-base titration method. The result of this qualitative analysis of the sample will turn pink if it contains sodium benzoate. Then the sodium benzoate wavelength optimization is carried out in the range of 200-400nm, and the optimum wavelength is 226nm. The method validation process is done by using four parameters namely linearity test, accuracy test, precision test, and LOD&LOQ. Based on the validation of the method, the correlation coefficient (R2) of 0.99563 indicates linear, recovery % is 97.58% in the range of 80-120%, RSD is 0.0454% which is ?2%, LOD is 0.33 ppm, and LOQ of 1.0996 ppm. Based on these results, it can be said that the method used is valid because all parameters meet the specified requirements. Analysis of the determination of levels using a UV-Vis Spectrophotometer instruments at wavelength 226nm using 5 samples, from the five samples obtained average rate of 90.639±0.0406. Analysis of the rate determination using UV-Vis Spectrophotometer instruments at 226nm wavelengths show that sample A has a concentration 92,243±0,039 ppm, sample B is 80,286±0,039 ppm, sample C is 99,04±0,063 ppm, sample D is 101,483±0,025 ppm, and sample E is 80,143±0,038 ppm. The content of sodium benzoate in soybean milk is following the requirements of BPOM RI regulation No.36 of 2013 concerning the maximum limit of food use, the use of sodium benzoate in fruit/vegetable juice products and non-fermented soybean products is 600ppm of food, with ADI 0-5mg/kg body weight.