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Sammy N.J. Longdong
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Environmental Science

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Sensitivitas ekstrak tanaman kemangi (Ocimum sactum) terhadap bakteri Aeromonas hydrophila Sambuaga, Mutiara E.; Longdong, Sammy N.J.; Manoppo, Henky
e-Journal BUDIDAYA PERAIRAN Vol 6, No 1 (2018)
Publisher : Universitas Sam Ratulangi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35800/bdp.6.1.2018.19520

Abstract

The research was conducted to appraise the antimicrobial activity of Ocimum sactum extract on A. hydrophila. Fresh basil was purchased from the local market,  put in plastic bags and brought to Laboratory of Fish Health, Environment, and Toxicology, Faculty of Fisheries and Marine Science Sam Ratulangi University.  The basil was cleaned and separated between trunk and leaf, and then macerated in alcohol 70% at room temperature for 24 hours. The extract was filtered using Whatman paper and evaporated using rotary evaporator. Paper disc (diameter: 6 mm) was immersed in the extract,  placed on TSA previously inoculated with A. hydrophila, and incubated at 28oC for 24 hours.  The result showed  that trunk and leaf extracted with alcohol 70% did not inhibit the grow of bacteria while aquades extract was able to inhibit growth of bacteria indicated by the present of clear zone around the paper discs with a diameter of 16.5 mm for leaf and 20.5 mm for basil trunk. The ability of these two extracts to inhibit the growth of bacteria was categorized as strong. Thus, aquades extract of basil was able to inhibit the growth of pathogenic bacteria. Keywords: Ocimum sactum, antimicrobial, disc diffusion method, medicinal plant,  inhibition zone
Isolasi bakteri probiotik Lactobacillus sp dari usus ikan mas (Cyprinus carpio) Novitarizky, Intan A.; Manoppo, Henky; Longdong, Sammy N.J.
e-Journal BUDIDAYA PERAIRAN Vol 6, No 2 (2018)
Publisher : Universitas Sam Ratulangi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35800/bdp.6.2.2018.20492

Abstract

The objective of research was to isolate the probiotic bacteria from intestine of carp.  Isolation was performed by weighing 1 g of intestine, crushed and inserted in a reaction tube containing 9 mL of NaCl then centrifuged. Supernatant was taken and spread over the agar medium by scatter method. The bacteria-stocked medium was then incubated in an incubator at 28°C for 24-48 hours. The growing colonies were re-isolated on the agar medium to obtain pure colonies. The bacteria were then identified by morphological observation, resulting pigment and gram staining. Five nile tilapia (sized 8 – 10 cm) were used in pathogenicity test and placed in the aquarium. Fish were injected with 0.2 ml of Lactobacillus solution containing 1x107 CFU/mL via intraperitoneal injection. The results showed that the bacteria Lactobacillus sp had rod-shaped characteristics, white pigment and gram positive and  not pathogenic, which means it can be used as probiotic for aquaculture.Keywords : Lactobacillus sp, carp, probiotic, pathogenicity test
Pengaruh penambahan karbon aktif terhadap pertumbuhan dan kelangsungan hidup lele (Clarias gariepinus) dalam wadah terkontrol Tamba, Ermaulina; Mokolensang, Jeffrie F.; Pangkey, Henneke; Longdong, Sammy N.J.; Undap, Suzanne L.
e-Journal BUDIDAYA PERAIRAN Vol 7, No 1 (2019)
Publisher : Universitas Sam Ratulangi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35800/bdp.7.1.2019.24389

Abstract

This study aimed to determine the effect of addition of different activated carbon on growth of catfish, feeding efficiency, and feeding conversion ratio and survival rate. This research was carried out in the Aquaculture Technology laboratory, Faculty of Fisheries and Marine Science. The media test was an aquarium with the size of 60 x 40 x 40 cm placed in laboratory. The experimental design was a completely randomized design with 5 treatments and 3 repetitions. The charcoal used in this experiment was A: 1%, B: 2%, C: 3%, D: 4%, E: without charcoal. The observed variables were total growth, relative growth, daily growth, feeding efficiency, feed conversion ratio, and survival rate. The results of statistical analysis on growth parameters indicated the same effect on the growth of 4-6 cm catfish juvenile. The highest weight gain was achieved in treatment C (2,62±0,1), which was pellet feeding with a composition of 3% charcoal, as well as relative growth (413,47±36,3), and daily growth (1.3±0,2). The treatment showed significant effect on efficiency. The highest feed conversion ratio was in treatment E (2,06±1,4), which was pellet feeding with 0% charcoal, and the lowest in treatment C (0,76±0,1). This study concluded that addition of charcoal 1%, 2%, 3%, 4% and without charcoal had the same effect in triggering 4-6 cm catfish growth.Keywords: Active carbon, catfish, growth, feeding efficiency, feeding conversion
Kajian kualitas air pada area budidaya kurungan jaring tancap (KJT) di Danau Tutud Desa Tombatu Tiga Kecamatan Tombatu Kabupaten Minahasa Tenggara Tokah, Christian; Undap, Suzanne L.; Longdong, Sammy N.J.
e-Journal BUDIDAYA PERAIRAN Vol 5, No 1 (2017)
Publisher : Universitas Sam Ratulangi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35800/bdp.5.1.2017.14837

Abstract

The aim of this study was to measure and assess the physical and chemical parameters of water quality in the area of ​​fixnet cage cultureat Lake Tutud Tombatu TigaVillagewhich included temperature, pH, DO, TDS, NO3, NO2, NH3 and PO4 in a different time.This research was conducted from August to November 2016. The research activities consisted of direct measurements in the field (in situ) using a Horiba instrument and laboratory analysis (ex situ) at the Agency for Industrial Research and Development Research Institute of Standardization and Industrial Manado. Determination points were done by purposive sampling which refers to the physiographic location wherever possible in order to represent or describe these waters.Water quality measured at4 stations using a Horiba at a depth of 0.5 meters from the bottom of the lake.Station I represented Inlet water, Station II where the cultivation A, Station III where the cultivation B and Station IVwhere no cultivation. The data obtained and collected were primary data i.e., measurement of physical and chemical parameters of water quality as well as watching for signs of sick fish, dead fish, and the growth of farmed fish.The results showed the water temperature ranged between 28-29 ° C, TDS 0266-0412 mg/L and chemical parameters for dissolved oxygen 2-5 mg/L, pH 7-8 and for the results of laboratory analysis, N03(0.7 - 6.3 mg/L), NO2(0001-0002 mg/L), NH3 (0180-1920 mg/L), PO4(0020-0209 mg/L). In general, the existence of water quality of lake Tutud were still in good condition except for NH3 concentrationthat exceeded quality standard limitsof Government Regulation No. 82 of 2001. Basically lake Tutud can still be used for fish farming, but the addition of new cages were not recommended.   Keywords: Lake Tutud,water quality, physical parameters, chemical parameters, fix net cage
Konsentrasi Hambatan Minimum ekstrak Portulaca grandiflora terhadap penyakit Motile Aeromonad Septicaemia Lauluw, Dafid; Kreckhoff, Reni L.; Longdong, Sammy N.J.; Mantiri, Desy M.; Watung, Juliaan Ch.; Tumbol, Reiny A.
e-Journal BUDIDAYA PERAIRAN Vol 6, No 2 (2018)
Publisher : Universitas Sam Ratulangi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35800/bdp.6.2.2018.20659

Abstract

The objective of this study was to determine the minimum concentration of extract in inhibiting motile motile aeromonad septicaemia. The material used was the stems and leaves of the plant P. grandiflora as much as 10 g. The crushed simplicia was then macerated with aquades with a ratio of 1:10,  then filtered with filter paper and, put in an Erlenmeyer, and evaporated in an incubator at temperature of 30°C. The sensitivity test was performed by placing a paper disc containing extract on the agar medium that had been scratched with bacterial colonies. Clear zone formed around the paper disc was compared with standard classification table for clarifying the inhibition level.  The results showed that stem and leaf extract had inhibition zone against Aeromonas hydrophila bacteria that caused motile aeromonad septicaemia. The best result was obtain at concentration of extract 3.25 mg / L) with an average diameter of 22.67 mm and for leaf extract had a moderate to strong zone for all treatments. These proved that stem and leaf extract of P. grandiflora plant had antibacterial activity and can be used as control of MAS disease caused by A. hydrophila bacteria. Keywords:  Portulaca grandiflora plant, motile aeromonad septicemia,  minimum       inbibition concentration
Efikasi ekstrak tanaman obat Binahong Anredera cordifolia (Ten) Steenis untuk meningkatkan pertumbuhan ikan Nila Oreochromis niloticus Wantah, Monika M.; Longdong, Sammy N.J.; Kreckhoff, Reni L.; Mantiri, Desy M.H.; Tumbol, Reiny A.; Manoppo, Henky
e-Journal BUDIDAYA PERAIRAN Vol 6, No 2 (2018)
Publisher : Universitas Sam Ratulangi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35800/bdp.6.2.2018.20502

Abstract

The aim of this research was to study the effect of Binahong extract on the growth of Nile tilapia. A number of 225 fish measuring 10-12 cm in length with an average weight of 15 g was obtained from Fishery Development Center and Training, Tateli. The fish were put  in oxygenated plastic bags and transported to Laboratory of Aquaculture Technology. The fish was kept in aquarium equipped with an aerator with a density of 15 fish per aquaria. The test material used was the stem of Binahong plant stem, macerated with alcohol 70% for 3 days. The extract was then filtered with whatman filter paper and extract was concentrated using a Rotary Evaporator. The extract was further diluted to obtain  concentrations of 12.5, 25, 50, and 100 mg / mL Binahong  extract as treatment was given to the fish after mixed first into the pellet. The fish was fed with treatment feed for 30 days. Control feed was not supplemented with binahong extract. Growth data was measured at day 30 after treatment. The results showed that the extract of binahong had significant effect on fish growth (p <0.01) with the best result achieved at concentration of 100 mg/mL. In conclusion, supplementation of  binahong extract into fish feed could improve growth of  fish in which the best growth was obtained in fish fed diet containing 100 mg/mL of binahong extract.Keywords: Medicinal plants, tilapia, Anredera cordifolia, growth, maceration
Pertumbuhan dan kelangsungan hidup ikan nila Oreochromis niloticus yang dikultur pada sistem akuaponik dengan kepadatan biofilter kangkung yang berbeda Gerung, Pramulya R.A; Mudeng, Joppy D.; Salindeho, Indra R.N.; Longdong, Sammy N.J.; Pangkey, Henneke; Rumengan, Inneke F.M.
e-Journal BUDIDAYA PERAIRAN Vol 10, No 2 (2022)
Publisher : Universitas Sam Ratulangi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35800/bdp.10.2.2022.38562

Abstract

This research was conducted to determine the optimal density of kale plants as biofilter in an aquaponic system that can trigger the highest growth and survival of tilapia. The experiment was designed in a completely randomized design (CRD) with 3 treatments and 3 replications so that there were 9 experimental units. The factor tested was the different density of kale plants as biofilter in the aquaponics system, where treatment A: 6 kale plants/0,24m2, B: 12 kale plants/0,24m2, and  K: without kale plants (as control). This study was conducted for 28 days.  Collected data  included weight, length and number of fish, which were then converted to  absolute growth rate (weight and length) , daily growth rate, survival rate and kale plant growth. Data  were analyzed using ANOVA  and then Least Significance Differences (LSD) for  further analysis.   The results  showed that the different density of  kale plants had no significant effect on absolute growth rate (weight and  length)  and daily growth rate, but had a significant effect on the survival rate of fish. The LSD results  showed that density of 6 kale plants/0,24m2 (A) were not significantly different from that of 12 individuals kale plants/0,24m2 (B), but both treatments were significantly different from the control (K).  The range of ammonia  was between 0.29 – 0.91 mg/l, nitrite 0.55 – 1.06 mg/l, and nitrate 10 - 47 mg/l.
Inventarisasi dan identifikasi ektoparasit yang menginfeksi benih ikan Nila (Oreochromis niloticus) Massora Dudung; Reni L. Kreckhoff; Reiny A. Tumbol; Sammy N.J. Longdong; Winda M. Mingkid
e-Journal BUDIDAYA PERAIRAN Vol. 11 No. 2 (2023): April - Desember
Publisher : Universitas Sam Ratulangi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35800/bdp.v11i2.49945

Abstract

This study aimed to identify the parasites in tilapia seeds cultivated by the Matelenteng Fish Cultivation Group in Tumaluntung Village, as well as to analyze the prevalence, incidence, dominance index and preference level of parasites in tilapia seeds. This research was conducted from May to July 2023 in Tumaluntung Village, Kauditan District, North Minahasa Regency, North Sulawesi Province. Parasite examination was carried out on 15 samples of tilapia seeds which included the skin, fins, and gills of the fish. The fish samples were taken from the Matelenteng fish cultivation location in Tumaluntung Village, Kauditan District, North Sulawesi Province. The fish were taken from 1 breeding pond and put into plastic bags containing water, then given oxygen, and then brought to the Aquaculture Technology Laboratory of FPIK Unsrat for examination. Before the examination was carried out, the test fish were put into a bucket and given aeration so that the tilapia seeds remained alive until they were examined. Next, the length of the tilapia seeds was measured using a ruler. Examination of the tilapia seed samples was carried out using the smear preparation method where the examined organ was scraped, and the results of the scraping were placed in a Petri dish before being smeared on a prepared glass slide. Parasite examination was conducted using a microscope with a magnification of 100x. The inventory and identification results found 2 types of parasites, namely Epistylis sp. with 13 individuals in 3 samples of tilapia seeds and Dactylogyrus sp. with 2 individuals in 2 samples of tilapia seeds infecting the tilapia seeds. The prevalence rate of Epistylis sp. parasites was obtained at 33.33% or generally, there is a mild infection. Dactylogyrus sp. with a prevalence rate of 13.33% is classified as often infected with an incidence rate at a very mild level. The preference level of parasites obtained a value of X2 hit 3.74 < X2 tab 9.210 indicating a difference in the preference level of parasites in the tilapia seed body organs. Keywords: Ectoparasites, prevalence, incidence, preference level
Analisis kandungan nitrogen dan fosfor pada sedimen Danau Tondano di area budidaya Toulimembet Yedieli Telaumbanua; Sipriana S. Tumembouw; Juliaan Ch. Watung; Suzanne L. Undap; Pankie L. Pangemanan; Sammy N.J. Longdong
e-Journal BUDIDAYA PERAIRAN Vol. 11 No. 2 (2023): April - Desember
Publisher : Universitas Sam Ratulangi

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

The aim of the research was to analyze the nitrogen and phosphorus content in sediments in the cultivation area of Toulimembet Village. This research method is "survey" determining sampling points "purposive sampling" taking into account that the research location is a fish cultivation area with a floating net system, and data is obtained using primary data and secondary data. Primary data collected were in situ measurements of water quality parameters (temperature, turbidity, TDS, pH, DO) in the Toulimembet Village cultivation area and sediment analysis (Nitrogen and Phosphorus) at the Manado Standardization Research Center Laboratory (BARISTAND). Secondary data was obtained from various sources such as journals and previous research reports. Based on research results in Toulimembet Village, Kakas District, Minahasa Regency, North Sulawesi Province, nitrogen (0.78-1.16%), phosphorus (0.04-0.08%), temperature (25.73-27.01°C), turbidity (4.03-4.90 NTU), TDS (0.160-0.164 g/l), pH (7.89-10.52), DO (7. 20-12.67 mg/L), ammonia (0.07 mg/L), phosphate (0.0030-0.0158 mg/L), nitrite (0.069-0.295 mg/L) are still suitable for cultivation activities. Keywords: Fish culture, water quality, floating net cage, eutrophication
Prevalensi dan insidensi ektoparasit pada udang vaname (Litopenaeus vannamei) di tambak CV. Pasifik Delyano Farm Leyda H. Mokoginta; Reni L. Kreckhoff; Reiny A. Tumbol; Sammy N.J. Longdong; Hariyani Sambali; Winda M. Mingkid
e-Journal BUDIDAYA PERAIRAN Vol. 11 No. 2 (2023): April - Desember
Publisher : Universitas Sam Ratulangi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35800/bdp.v11i2.51960

Abstract

This study aimed to inventory the parasites that attack vaname shrimp (Litopenaeus vannamei) and to determine and determine the prevalence and incidence rates of parasites that infect vaname shrimp. The research was carried out at CV. Pasifik Delyano Farm, Iyok Village, District. Bolangitang, Kab. North Mongondow. Parasite identification is carried out in the Laboratory of Aquaculture Technology FPIK Unsrat. The test animals used in this rstudy were vannamei shrimp with a weight ranging from 7-10 grams with a length of 10-15 cm. The number of shrimp samples taken was 10 in the pond. Methods for examining and observing ectoparasites were carried out on the gills, walking legs, swimming legs, and tail of vannamei shrimp and then identified to obtain prevalence and incidence rates. The observation results were then analyzed using prevalence and incidence rate formulas. Based on the research results, two types of parasites were found that infect shrimp, walking legs, swimming legs, and tail, namely Vorticella and Zootanium. Prevalence rates based on parasite type were obtained in Vorticella sp. 40% and Zoothamnium sp 30% in the category of infections that generally occur while the prevalence rate based on organs, swimming legs was higher (0.4%) than in tail (0.3%) and walking legs (0.2%). based on the level of parasite incidence in all observed shrimp organs, Zoothamnium sp. was higher than Vorticella sp.  Keywords: diseases, parasites, aquaculture, intensive cultivation
Co-Authors Gerung, Pramulya R.A Hariyani Sambali Henky Manoppo Henneke Pangkey Indra R.N. Salindeho Inneke F. M Rumengan Joppy Mudeng Juliaan Ch. Watung Kreckhoff, Reni L. Lauluw, Dafid Leyda H. Mokoginta Mantiri, Desy M. H Massora Dudung Mokolensang, Jeffrie F. Novitarizky, Intan A. Pankie L. Pangemanan Reiny A. Tumbol Reiny A. Tumbol Sambuaga, Mutiara E. Sipriana S. Tumembouw Suzanne L Undap Suzanne L. Undap Tamba, Ermaulina Tokah, Christian Wantah, Monika M. Winda M. Mingkid Winda M. Mingkid Yedieli Telaumbanua