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All Journal AQUATIC SCIENCE & MANAGEMENT e-Journal BUDIDAYA PERAIRAN JURNAL ILMIAH PLATAX KESMAS Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati
Reiny A. Tumbol
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Environmental Science Health Professions Public Health

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Identifikasi, Tingkat Insidensi, Indeks Dominasi dan Tingkat Kesukaan Parasit pada Sidat (Anguilla marmorata) Tumbol, Reiny A.; Longdong, Sammy N.; Kanoli , Tauvan A.
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 16, No 1 (2011): February 2011
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (445.222 KB) | DOI: 10.24002/biota.v16i1.66

Abstract

Penelitian ini dilakukan dengan bertujuan mengetahui jenis-jenis parasit, tingkat insidensi, indeks dominasi dan tingkat kesukaan parasit pada sidat (Anguilla marmorata). Pemeriksaan sampel Sidat dimulai tanggal 25 Mei−16 Juni 2009, di Laboratorium Kesehatan Ikan Balai Budidaya Air Tawar Tatelu. Sampel diambil secara acak dari kolam-kolam pembesaran sebanyak 50 ekor, ukuran panjang 30–45 cm. Pemeriksaan parasit pada Sidat terbagi atas pemeriksaan ektoparasit yaitu mata, mulut, kulit/sisik, insang dan sirip. Pemeriksaan endoparasit meliputi pemeriksaan lambung, usus, hati dan daging. Hasil identifikasi parasit yang menginfeksi Sidat adalah Trichodina sp, Lerneae sp, Myxobolus sp, Gyrodactylus sp, Oxyurida sp, Capillaria, Acanthocephalus sp dan Vorticella sp. Tingkat insidensi dari tiap spesies parasit adalah sebagai berikut; Trichodina sp 92%, Gyrodactylus sp 74%, Lerneae sp 26%, Capillaria sp 24%, Myxobolus sp 22%, Oxyurida sp 6%, Acanthocephalus sp 2% dan Vorticella 2%. Analisis indeks dominasi menunjukan bahwa ada satu jenis parasit yang mendominasi infeksi parasit-parasit yang ada pada organ-organ sampel yang diperiksa dan parasit tersebut adalah Trichodina Sp. Setiap jenis parasit memiliki tingkat kesukaan yang berbeda dalam menempati organ-organ tubuh Sidat.
Efikasi Bakasang sebagai imunostimulan pada pakan sidat (Anguilla marmorata) terhadap infeksi Aeromonas hydrophila Janis, Olvi A.P.; Tumbol, Reiny A.; Longdong, Sammy
e-Journal BUDIDAYA PERAIRAN Vol 4, No 2 (2016)
Publisher : Universitas Sam Ratulangi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35800/bdp.4.2.2016.13219

Abstract

The purpose of this study was to examine the effect of fish silages, bakasang as an immunostimulant in eel (Anguilla marmorata) feed against Aeromonas hydrophila infection. Three hundred elvers with the size of + 10 cm taken from Freshwater Aquaculture Institute (BBAT) Tatelu. The elvers were placed into 4 glass aquarium, each equipped with aerator and 1 for stock. Prior to the study, the eels were acclimatized for two weeks in order to adapt to new environmental condition. Two sets of researches were done: feeding the eels with additional bakasang with different doses for 6 weeks, which then further were challenged with Aeromonas hydrophila by immersion method. This study used a completely randomized design (CRD) with 4 treatments A (0 mL /kg diet), B (50 mL/kg diet), C (100 mL/kg diet), D (150 mL/kg diet). The growth data was taken by measuring the weight and length of the eels at the end of the period of feeding treatment while the mortality data of the eels were observed for 10 days after challenge test.  The results of analysis of variance showed that the resistance of the eels in treatment B (50 mL/kg diet), C (100 mL/kg diet) and D (150 mL/kg diet) significantly different compared with treatment A.  However, among the treatments B, C and D, there were no significant differences.  Furthermore, the best eel growth was observed in treatment B with an absolute growth of 1.14 g, followed by treatment of D with an absolute growth of 1.04 g. In treatment A the absolute growth was only 0.82 g while treatment C was 0. 88 g.  This suggests that the addition of bakasang in eels feed can promote the growth of eels as well as resistance against pathogen infection, thus served as immunostimulant. Keywords: fish silages, immunostimulant, eel,  Aeromonas hydrophila, growth
Suplementasi bakteri probiotik meningkatkan performa pertumbuhan dan efisiensi pakan benih ikan mas (Cyprinus carpio). Mudeng, Cindy C.; Manoppo, Henky; Lantu, Sartje; Kreckhoff, Reni L.; Tumbol, Reiny A.
e-Journal BUDIDAYA PERAIRAN Vol 8, No 1 (2020)
Publisher : Universitas Sam Ratulangi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35800/bdp.8.1.2020.27222

Abstract

Probiotic from carp intestines and its effects on increasing growth performance and feed efficiency had been investigated. Fish used were carp fingerlings weighing 2.33 g  in average. The fish were taken from the Tatelu Freshwater Aquaculture Fisheries Center.  Probiotics used were isolated from the intestines of carp. Before running the experiment, the fish were acclimatized for 10 days under laboratory conditions. During acclimatization the fish were fed commercial pellets without the addition of probiotics. The dose of feeding was 5% /bw/day, 2 times a day at 09:00 am and 16:00 pm. Furthermore, fish are stocked in 15 aquariums each measuring 60x40x40cm3 with a density of 20 fish/aquarium. Each aquarium was equipped with aeration and water pumps for recirculation. Uneaten feed and feces were removed through siphoning. Water exchange was done every 2-3 days.  After acclimatization, the fish were fed with pellet supplemented with probiotic at  concentration of A: without addition of probiotic; B: 1 x 106 cfu /mL; C: 1 x 107 cfu / mL; D: 1 x 108 cfu / mL; E: 1 x 109 cfu/mL. The fish were fed twice a day with feeding dose of 5% /bw/ day, for 21 days. Data collected consisted of daily growth rates, growth rates, feed efficiency and feed conversion ratio. The results of research showed that the addition of probiotics in feed had a very significant effect on growth, feed efficiency, and feed conversion ratio (P <0.01) with the best results achieved in administering probiotics as much as 1 x 108 cfu / mL. In conclusion, probiotic bacteria originating from the intestines of carp can be used to improve the growth performance of the fish itself.
Isolasi dan identifikasi bakteri patogen pada ikan nila (Oreochromis niloticus) yang dibudidayakan di Kecamatan Dimembe Kabupaten Minahasa Utara Tahun 2019 Jr, Arfiandi; Tumbol, Reiny A.
e-Journal BUDIDAYA PERAIRAN Vol 8, No 1 (2020)
Publisher : Universitas Sam Ratulangi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35800/bdp.8.1.2020.27229

Abstract

This study aimed to identify the type of pathogenic bacteria that attack tilapia (Oreochromis niloticus) cultivated in Dimembe District, North Minahasa Regency in 2019. The benefit of this research were to obtain information about the types of bacteria in tilapia (Oreochromis niloticus), so prevention efforts can be made or treatment of the disease. Tilapia as a research sample obtained from existing cultivation sites in the District of North Minahasa Dimembe, North Sulawesi Province. From each location 5-10 individuals were taken with a size of 5-12 cm. The samples were then taken to the laboratory for bacterial isolation. The media for growing bacteria was TSA (Tryptic Soya Agar). The results obtained were adjusted to the Bacterial Fish Pathogens identification book. The data obtained were analyzed descriptively through the presentation of tables and figures. Research results found the bacteria that infected tilapia in North Minahasa Regency including Aeromonas sp, Plesiomonas sp, Flavobacterium sp and Enterobacter sp. Regular and continuous monitoring of fish diseases is needed to monitor the spread of fish diseases, especially quarantine fish diseases in North Minahasa Regency.
Respon imun benih ikan mas, Cyprinus carpio, yang diberi pakan probiotik Lactobacillus sp. dengan konsentrasi berbeda Pangalila, Novelia; Manoppo, Henky; Tumbol, Reiny A.; Lumenta, Cyska; Kreckhoff, Reni L.; Warouw, Veibe
e-Journal BUDIDAYA PERAIRAN Vol 8, No 1 (2020)
Publisher : Universitas Sam Ratulangi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35800/bdp.8.1.2020.27492

Abstract

The research aimed to isolate probiotic bacteria from catfish intestine and assign the concentration of probiotic bacteria which effectively increase the activity of phagocytosis and total leukocytes of carp fingerlings. The fish was taken from Balai Pengembangan dan Pembinaan Pembudidayaan Ikan (BP3I) Tateli. Fish (average initial weight of 2.27 g) were acclimated for one week in 15 aquaria with a density of 25 fish/aquarium. One week after acclimatization, fish were fed with food added with probiotics with different concentrations, namely 1x106, 1x107, 1x108, 1x109 cfu/mL for four weeks. The dose of feed was 5% /body weight/day and feeding time was at 08.00 and 17.00. Data collected were phagocytosis and total leukocyte count. The results showed that supplementation of probiotic significantly increased phagocytic activity and total leukocytes (p<0.01). The best phagocytic activity and total leukocytes were achived in fish fed diet added with probiotics 1x108 cfu/mL. It can be concluded that supplementation of  probiotic onto feed can increase phagocytic activity and total leukocytes of carp.
Konsentrasi Hambatan Minimum ekstrak Portulaca grandiflora terhadap penyakit Motile Aeromonad Septicaemia Lauluw, Dafid; Kreckhoff, Reni L.; Longdong, Sammy N.J.; Mantiri, Desy M.; Watung, Juliaan Ch.; Tumbol, Reiny A.
e-Journal BUDIDAYA PERAIRAN Vol 6, No 2 (2018)
Publisher : Universitas Sam Ratulangi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35800/bdp.6.2.2018.20659

Abstract

The objective of this study was to determine the minimum concentration of extract in inhibiting motile motile aeromonad septicaemia. The material used was the stems and leaves of the plant P. grandiflora as much as 10 g. The crushed simplicia was then macerated with aquades with a ratio of 1:10,  then filtered with filter paper and, put in an Erlenmeyer, and evaporated in an incubator at temperature of 30°C. The sensitivity test was performed by placing a paper disc containing extract on the agar medium that had been scratched with bacterial colonies. Clear zone formed around the paper disc was compared with standard classification table for clarifying the inhibition level.  The results showed that stem and leaf extract had inhibition zone against Aeromonas hydrophila bacteria that caused motile aeromonad septicaemia. The best result was obtain at concentration of extract 3.25 mg / L) with an average diameter of 22.67 mm and for leaf extract had a moderate to strong zone for all treatments. These proved that stem and leaf extract of P. grandiflora plant had antibacterial activity and can be used as control of MAS disease caused by A. hydrophila bacteria. Keywords:  Portulaca grandiflora plant, motile aeromonad septicemia,  minimum       inbibition concentration
Efikasi ekstrak tanaman obat Binahong Anredera cordifolia (Ten) Steenis untuk meningkatkan pertumbuhan ikan Nila Oreochromis niloticus Wantah, Monika M.; Longdong, Sammy N.J.; Kreckhoff, Reni L.; Mantiri, Desy M.H.; Tumbol, Reiny A.; Manoppo, Henky
e-Journal BUDIDAYA PERAIRAN Vol 6, No 2 (2018)
Publisher : Universitas Sam Ratulangi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35800/bdp.6.2.2018.20502

Abstract

The aim of this research was to study the effect of Binahong extract on the growth of Nile tilapia. A number of 225 fish measuring 10-12 cm in length with an average weight of 15 g was obtained from Fishery Development Center and Training, Tateli. The fish were put  in oxygenated plastic bags and transported to Laboratory of Aquaculture Technology. The fish was kept in aquarium equipped with an aerator with a density of 15 fish per aquaria. The test material used was the stem of Binahong plant stem, macerated with alcohol 70% for 3 days. The extract was then filtered with whatman filter paper and extract was concentrated using a Rotary Evaporator. The extract was further diluted to obtain  concentrations of 12.5, 25, 50, and 100 mg / mL Binahong  extract as treatment was given to the fish after mixed first into the pellet. The fish was fed with treatment feed for 30 days. Control feed was not supplemented with binahong extract. Growth data was measured at day 30 after treatment. The results showed that the extract of binahong had significant effect on fish growth (p <0.01) with the best result achieved at concentration of 100 mg/mL. In conclusion, supplementation of  binahong extract into fish feed could improve growth of  fish in which the best growth was obtained in fish fed diet containing 100 mg/mL of binahong extract.Keywords: Medicinal plants, tilapia, Anredera cordifolia, growth, maceration
HUBUNGAN PERILAKU HIDUP BERSIH DAN SEHAT (PHBS) IBU DENGAN KEJADIAN DIARE PADA BAYI USIA 1-4 TAHUN DI DESA KEICI KECAMATAN IBU KABUPATEN HALMAHERA BARAT 2018 Labudo, Lipna; Umboh, Jootje M. L.; Tumbol, Reiny A.
KESMAS Vol 7, No 5 (2018): Volume 7, Nomor 5, September 2018
Publisher : Sam Ratulangi University

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Diare merupakan penyakit dimana seseorang kalau sedang buang air besar dengan konsistens cair dan juga bahkan dapat berupa air dan sering yang biasanya tiga kali atau lebih dalam satu hari. Tujuan penelitian ini untuk mengetahui hubungan lingkungan dengan kejadian diare pada balita. Untuk mengetahui hubungan antara Perilaku Hidup Bersih dan Sehat, Rumah tangga dengan kejadian penyakit diare pada anak  usia 1 sampai 4 tahun di Desa Kie-ici Kecamatan Ibu kabupaten halmahera barat. Metode penelitian yang digunakan pada penelitian analitik dengan menggunakan pendekatan study  potong silang. Jumlah sebanyak 70 responden. Adapun data dikumpulkan dengan cara wawancara, juga kuesioner yang nantinya dianalisis dengan menggunakan uji chi-square (CI=95%, α=0,05) Adapun hasil analisis data primer diketahui bahwa hubungan menggunakan air bersih dengan kejadian diare pada Bayi 1-4 Tahun menunjukkan nilai p value= 0,032 yang berarti terdapat hubungan. Hubungan kebiasaan cuci tanggan dengan menggunakan sabun dengan kejadian diare pada balita usia 1-4 tahun menunjukkan nilai p value =0,012 yang berarti terdapat hubungan. Kata Kunci : menggunakan air bersih, kebiasan mencuci tanggan PHS ABSTRACTDiarrhea is a disease where a person is defecating with liquid consistency and can even be water only and often, usually three times or more in one day. The aim of this research is to know the relation between environment and diarrhea occurrence in under five years old. To find out the relationship between Clean and Healthy Living Behavioral Behavior (PHBS) with the incidence of diarrheal disease in children aged 1 to 4 years old in Kie-ici Village, Ibu district of halmahera district. The research method used is analytic research using cross sectional study approach. Total of 70 respondents. The data were collected through interviews using questionnaires which were later analyzed by using chi-square test (CI = 95%, α = 0.05). The primary data analysis result showed that the relationship between clean water and diarrhea occurrence in Infant 1-4 Years shows the value of p value = 0,032 which means there is a relationship. The relationship of hygiene with soap use with diarrhea occurrence in toddler age 1-4 years shows the value of p value = 0,012 which means there is a relationship. Keywords:  use clean water, waste management, PHBS responsive washing habits
Fish disease mapping in North Sulawesi Province Manumpil, Silvana; Tumbol, Reiny A.; Lasut, Markus T.
AQUATIC SCIENCE & MANAGEMENT Vol 3, No 2 (2015): Oktober
Publisher : Universitas Sam Ratulangi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35800/jasm.3.2.2015.14044

Abstract

Title (Bahasa Indonesia): Pemetaan penyakit ikan di Provinsi Sulawesi Utara This study aims to determine fish diseases and the causative pathogens, and to map the spread of the diseases in fish farms in North Sulawesi province. The organisms studied are freshwater and salt water fish. Fish samples were taken randomly both healthy fish and fish showing clinical signs of infections. Fish samples were randomly taken, 3 fishes from each sampling location, and only 1 fish farm was selected from each regency/city. Salt water fish were taken only from regency/city that has fish farm. To support the fish disease data, some water quality parameters were also measured in situ such as: temperature, pH, dissolved oxygen (DO), brightness and ammonia concentration. Results showed that the fish samples were infected with pathogenic parasites, bacteria and viruses. The bacteria were Vibrio carchariae, Vibrio mimicus, Vibrio damsel, Vibrio alginolyticus, Aeromonas hydrophilla, Pseudomonas sp, Vibrio sp, Aeromonas sp, Flexibacter sp, Pseudomonas flourescens. A. hydrophilla is the dominant species of bacteria in all freshwater fish samples from all sampling locations. The parasites were Dactylogirus sp, Trichodina sp, Ichthyopthirius multifiliis, Argulus sp, Lernea sp. Parasites Trichodina sp is the dominant parasite species found. The viruses were VNN virus (Viral Nervous Necrosis) KHV virus (Koi Herpes Virus). Virus NN virus was found only in grouper cultured in the floating net of Talengen village, district Sangihe Islands. Penelitian ini bertujuan untuk mengetahui jenis-jenis penyakit ikan dan patogen penyebabnya serta memetakan penyebaran penyakit ikan di lokasi budidaya yang ada di provinsi Sulawesi Utara. Sampel yang digunakan adalah ikan air tawar dan air laut. Sampel ikan diambil secara acak baik ikan yang sehat maupun ikan yang memperlihatkan gejalah terinfeksi penyakit. Tiga ekor ikan sampel diambil dari setiap lokasi, dimana dipilih 1 lokasi budidaya di tiap kabupaten/kota. Sampel ikan air laut hanya diambil pada kabupaten/kota yang memiliki lokasi budidaya air laut. pengukuran beberapa parameter kualitas air juga dilakukan secara in situ seperti: suhu, pH, oksigen terlarut (DO), kecerahan dan kandungan amoniak. Hasil penelitian memperlihatkan bahwa sampel ikan yang diperiksa terinfeksi pathogen parasit, bakteri dan virus. Jenis bakteri patogen yang ditemukan adalah: Vibrio carchariae, Vibrio mimicus, Vibrio damsel, Vibrio alginolyticus, Aeromonas hydrophilla, Pseudomonas sp, Vibrio sp, Aeromonas sp, Flexibacter sp, Pseudomonas flourescens. Bakteri Aeromonas hydrophilla merupakan jenis bakteri yang paling dominan yang ditemukan di semua sampel ikan air tawar di lokasi-lokasi budidaya tempat pengambilan sampel. Jenis-jenis parasit yang ditemukan adalah: Dactylogirus sp, Trichodina sp, Ichthyopthirius multifiliis, Argulus sp, Lernea sp. Jenis parasit yang dominan adalah Trichodina sp. Jenis virus yang ditemukan adalah: virus VNN (Viral Nervous Necrosis), virus KHV (Koi Herpes Virus). Virus VNN ditemukan hanya pada sampel ikan kerapu yang dibudidayakan di jaring apung Kampung Talengen, Kabupaten Kepulauan Sangihe.
Penggunaan bakteri probiotik untuk pencegahan infeksi bakteri Streptococcus agalactiae pada ikan Nila, Oreochromis niloticus Umasugi, Aisin; Tumbol, Reiny A.; Kreckhoff, Reni L.; Manoppo, Henky; Pangemanan, Novie P.L.; Ginting, Elvi L.
e-Journal BUDIDAYA PERAIRAN Vol 6, No 2 (2018)
Publisher : Universitas Sam Ratulangi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35800/bdp.6.2.2018.20556

Abstract

This study aimed to evaluate the effect of probiotic on growth and survival of tilapia (Oreochromis niloticus) against Streptococcus agalactiae infection. The test materials used were commercial probiotic bacteria and Streptococcus agalactiae. The probiotic bacteria were administered by mixing into the feed with a dose of 0 mL (without probiotics), 10 mL / kg of feed, 15 mL / kg of feed and 20 mL / kg of feed.  Feed was given 3 times a day at 08.00, 12.00 and 16.00 for 21 days. After that, the challenge test was done with Streptococcus  agalactiae bacteria by cohabitation. Bacteria with a density of 107 cells / mL were mixed into the water and allowed for 2 hours. The result showed that B treatment (10 mL / kg of feed) gave the best result with 93,33 percent of  survival rate. Statistical analysis showed that the survival of fish in treatments B, C and D was significantly different from treatment A (P <0.1). However, there was no significant difference between treatments B, C, and D. It was also found that the addition of probiotic in feed did not affect the growth of Nile tilapia (Orechromis niloticus). Keywords : Probiotic bacteria,  nile tilapia,  Streptococcus  agalactiae, growth,  infection
Co-Authors Aylifia S. Somba Bella, Sintia S. Cyska Lumenta Deiske A. Sumilat Diane J. Kusen Edwin D Ngangi Erly Y. Kaligis, Erly Y. Fitje Losung Galih Arif Kusuma Ginting, Elvi L. Hengky J. Sinjal Henky Manoppo Henky Manoppo Henneke D. Pangkey Henneke Pangkey Henny A. Dien Hutagaol, Anggun F. I.F.M Rumengan jeane T Sumaraw Jimmy Mamuaya John L. Tombokan Jootje M. L. Umboh Jr, Arfiandi Juliaan Ch. Watung Kanoli , Tauvan A. Kreckhoff, Reni L. Labudo, Lipna Lauluw, Dafid Longdong, Sammy N. Mantiri, Desy M. H Manumpil, Silvana Markus T. Lasut Massora Dudung Maweikere, Fabiola C. Merlyn D. Manurung Mokolensang, Jeffrie F. Monijung, Revol D. Mudeng, Cindy C. Ockstan J. Kalesaran Olvi A.P. Janis, Olvi A.P. Pangalila, Novelia Pangemanan, Novie P. L. Pangemanan, Novie P.L. Paputungan, Firgiawan Prislawati Paputungan Ratusmanga, Jihan Remy E. P. Mangindaan Sammy Longdong, Sammy Sammy N. J. Longdong Sammy N. Longdong Sammy N.J. Longdong Sartje Lantu Shifa A. Schram Sinubu, Wulan V. Sipriana S. Tumembouw Steelma V. Rantung Suria Darwisito, Suria Suzanne L Undap Suzanne L. Undap Tauvan A. Kanoli Umasugi, Aisin Undi, Chintia S. Veibe Warouw Wantah, Monika M. Winda M. Mingkid