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KOLONI JAMUR ANTAGONIS Trichoderma spp PADA BEBERAPA MEDIA TUMBUH SECARA IN VITRO Haperidah Nunilahwati; Yani Purwanti; Khodijah .; Laili Nisfuriah; Joni Philep Rompas
JURNAL TRIAGRO Vol 2, No 2 (2017): Volume 2 No.2 Juli – Desember 2017
Publisher : Universitas Tridinanti Palembang

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Abstract

Trichoderma is an antagonist biological agent in some fungal pathogens that cause many plant diseases in farmland. The effort to multiply Trichoderma fungi in vitro may use some media material. The appropriate medium is a prerequisite for studying microorganisms in different environments. The study aims to determine the development of Trichoderma fungal colonies in a macroscopic way in some media. The research was conducted in the laboratory of Plant Pest and Disease Faculty of Agriculture Palembang University from September to November 2017. The research was carried out by stages of preparation of isolates, media preparation and Trichoderma fungus inoculation on potato media, cassava media, sweet potato media, rice media, corn and green bean media. Observation of macroscopic colony development was done 6 days after inoculation (hsi), ie density and colony color, then identified, and data were analyzed descriptively. The results showed that Trichoderma fungi developed in potato media, cassava media, sweet potato media, rice media, corn media and green bean media. Trichoderma fungal colonies on potato media, sweet potato media, green bean medium is more dense than in cassava media, rice media and corn media. Trichoderma fungal colonies form a circle with clear boundaries, colony color was dark green and bright green with white side colonies on all media.Keywords: Fungal colonies, biological agents, Trichoderma, media, in vitro
Efek Bionematisida terhadap Serangan Nematoda Puru Akar Meloidogyne incognita (Koffoid & White) Chitwood pada Beberapa Takaran Yani Purwanti; Haperidah Nunilahwati; Khodijah Khodijah
Seminar Nasional Lahan Suboptimal 2018: Prosiding Seminar Nasional Lahan Suboptimal "Tantangan dan Solusi Pengembangan PAJALE dan Kela
Publisher : Pusat Unggulan Riset Pengembangan Lahan Suboptimal (PUR-PLSO) Universitas Sriwijaya

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Abstract

Purwanti et al, 2019. Bionematicidal Effects on the Attack of Root-Knot Nematode Meloidogyne incognita (Koffoid & White) Chitwood at Several Doses. pp. 198-205.Biological control by utilizing antagonistic fungi is an alternative control of root-knot nematode (RKN) which does not damage the environment.  Giving organic material to the soil can increase soil fertility and support the success of biological control.  Bionematicides in the form of manure compost enriched with antagonistic fungi at the right dosage are expected to be able to control the attack of root-knot nematodes and increase tomato growth and production.  The aim of the study wass to determine the effect of bionematicide treatment on chicken manure compost enriched with antagonistic fungi from South Sumatra lowland vegetable crops at various doses in controlling the attack of root-knot nematodes on tomato plants. The bionematicide in the form of  chicken manure compost with Aspergillus 2 fungi and Trichoderma  has a high potential in suppressing root-knot nematode attacks.  Increased bionematicidal dose decreases the amount of nodule formed at the roots of tomatoes. Bionematisidal treatment in the form of chicken manure enriched with Aspergillus 2 and Trichoderma fungi at a rate of  300 g  10 - kg of soil produced the lowest amount of the purse on the root with the highest growth and tomato production.
Pengaruh Jamur Entomopatogen Rhizosfer Pertanaman terhadap Mortalitas Serangga Umpan Omphisa fuscidentalis (Lepidoptera: Pyralidae) di Laboratorium Haperidah Nunilahwati; Yani Purwanti; Laili Nisfuriah; Frank Sinatra
Seminar Nasional Lahan Suboptimal 2019: Prosiding Seminar Nasional Lahan Suboptimal “Smart Farming yang Berwawasan Lingkungan untuk Ke
Publisher : Pusat Unggulan Riset Pengembangan Lahan Suboptimal (PUR-PLSO) Universitas Sriwijaya

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Nunilahwati H, Purwanti Y, Nisfuriah L, Sinatra F.  2019. Effect of entomopathogenic fungi from the rhizosphere of plants on the mortality of insect bait omphisa fuscidentalis (Lepidoptera: Pyralidae) in laboratory. In: Herlinda S et al. (Eds.), Prosiding Seminar Nasional Lahan Suboptimal 2019, Palembang  4-5 September 2019. pp. 246-253. Palembang: Unsri Press. Entomopathogenic fungi are organisms that live as insect parasites and are used in environmentally friendly biological control in an effort to reduce the use of synthetic insecticides. The purpose of this study was to determine the effect of plant rhizosphere entomopathogenic fungi on O. fuscidentalis insect bait mortality in the laboratory. The results of the study can provide information about O. fuscidentalis insect larvae which can be used as bait entomopathogenic fungi for insects. Soil samples used to trap entomopathogenic fungi were taken by purposive sampling from the long bean crop rhizosphere in the Ogan Komering Ilir Regency. Fungi that infect bait insects are cultured in PDA media, incubated for 7 days and then identified. Mortality of bait insect larvae was observed at intervals of 3 days namely 3, 6, 9, 12 and 15 days after larval infestation in soil samples. The results showed the highest average mortality was on observation day 9 of 56% and lowest 0% on observation day 3. Entomopathogenic fungi that infect larvae of O. fuscidentalis bait insects were Beauveria bassiana and Metarhizium sp. O. fuscidentalis insect larvae can be used as bait insects for entomopathogenic fungi from rhizosphere of plants in the laboratory.Keywords: biological control, mortality, Omphisa fuscidentalis,  rizosphere
Efek Takaran Pupuk Hayati terhadap Pertumbuhan dan Produksi Caisim (Brassica juncea L.) Haperidah Nunilahwati; Neni Marlina; Yani Purwanti; Laili Nisfuriah; Ida Aryani; Rosmiah Rosmiah; Zulfakar Zulfakar
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Publisher : Pusat Unggulan Riset Pengembangan Lahan Suboptimal (PUR-PLSO) Universitas Sriwijaya

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Nunilahwati H, Marlina N, Purwanti Y, Nisfuriah L, Aryani I, Rosmiah R, Zulfakar Z. 2022. Effect of dose of biofertilizer on growth and production of caisim (Brassica juncea L.). In: Herlinda S et al. (Eds.), Prosiding Seminar Nasional Lahan Suboptimal ke-10 Tahun 2022, Palembang 27 Oktober 2022. pp. 226-233.  Palembang: Penerbit & Percetakan Universitas Sriwijaya (UNSRI).Biofertilizer are live microorganisms that are mixed into the soil as inoculants to help provide certain nutrients for plants. This study aimed to determine the effect of biofertilizer doses on the growth of mustard greens (Brassica juncea L.). The study was carried out in Seberang Ulu II District, Palembang City, South Sumatra from February to May 2021. The method used was a Non-Factorial Randomized Block Design (RAK) with 6 (six) treatments and 4 (four) replications with treatment H0 = 0 kg/ha, H1 = 20 kg/ha (0.05 g/polybag), H2 = 40 kg/h (0.10 g/polybag), H3 = 60 kg/ha (0.15 g/polybag), H4 = 80 kg/ha (0.20 g/polybag) and H5 = 100 kg/ha (0.25 g/polybag). The results of the diversity analysis showed that the effect of the dose of biofertilizer applied to caisim on plant height (cm), top root weight (g), and bottom root weight (g) had a significant effect, while plant height (cm) and leaf width (cm) had a very significant effect. The application of biofertilizer of 20, 40, 60 kg/ha, and 100 kg/ha on caisim plants showed lower growth and production than the application of 80 kg/ha of biofertilizer. The dose of biofertilizer 80 kg/ha is the best treatment for the growth and production of caisin mustard plants weighing 56.75 g/polybag.