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All Journal Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences Jurnal Kedokteran Brawijaya Medical Journal of Indonesia Research Journal of Life Science Program Kreativitas Mahasiswa - Penelitian INDONESIAN JOURNAL OF PHARMACY MNJ (Malang Neurology Journal) Majalah Kesehatan FKUB
Diana Lyrawati
Laboratorium Farmasi Fakultas Kedokteran Universitas Brawijaya Malang
Agriculture, Biological Sciences & Forestry Education Environmental Science Medicine & Pharmacology Neuroscience Public Health

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Journal : Medical Journal of Indonesia

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Cloning and expression of pab gene of M. tuberculosis isolated from pulmonary TB patient in E.coli DH5α M. Raras, Tri Y.; Lyrawati, Diana
Medical Journal of Indonesia Vol 20, No 4 (2011): November
Publisher : Faculty of Medicine Universitas Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (4108.055 KB) | DOI: 10.13181/mji.v20i4.458

Abstract

Background: Mycobacterium tuberculosis antigen38 is a potent serodiagnostic agent containing two M. tuberculosisspecific B-cell epitopes. The high price of imported diagnostic agents hinders realization of fast clinical TB diagnosis in developing countries. Therefore, we produced recombinant antigen38 (recAg38M) from M. tuberculosis local strain, which might be used to produce economical tuberculosis serodiagnostic kit.Methods: Pab gene that was isolated from pulmonary TB patient in Malang was cloned into a plasmid vector (pGEMTeasy) to construct pMB38. The E.coli DH5α clone carrying pMb38 was selected on X-gal medium. The expression of pab was mediated using pPRoExHTc under the control of Trc promoter and E.coli DH5α as host.Results: Alignment of the pab sequence from the white E.coli DH5α clones with that of M. tuberculosis H37Rv showed 98% homology. The recombinant protein in which the signal peptide has been deleted to prevent the protein being secreted into medium was found in the cytoplasm.Conclusion: pab gene of M. tuberculosis isolated from a TB patient could be expressed in heterologous system in E.coliDH5α. (Med J Indones 2011; 20:247-54)Keywords: Mycobacterium tuberculosis, Pab gene expression, recombinant antigen38
Co-Authors Alifia Putri Febriyanti Aswaty Nur Beta Herilla Sekti Debby Shintiya D Desie Suci Permata Sari Devi Anggraeni Pramulawati Djala, Fany Lairin Edi Widjajanto Elhemeidy, Rehab Mohammed Mustofa Ellen Natalia Faridha Khaira H Firman Mulyo Wicaksono Fitria Nurindro Rahmahani Habiba Aurora Herawati Sudoyo Herin Mawarti Herlina Y Handoko Hidayat Sujuti Imam Sarwono Indah Dina Maritha Juniartha, Putu Kenichi Yano Krisna Bayu Tambunan M Aris Widodo M. Rasjad Indra Mahfud, Rania Arif Martina Kurnia Rohmah Masruroh Rahayu, Masruroh Mohamad Rasjad Indra Mudjiwijono Handaru Muhammad Rasjad Indra Neshya Ruriana Putri, Neshya Ruriana Nia Kurnianingsih Noorma Lukitasari Novita Fahrianti Putri Nur Lailatul Fitria Nur Samsu Nurdiana Nurdiana Oktavia Rahayu Adianingsih Retty Ratnawati Retty Ratnawati Rudy Salam, Rudy Sangkot Marzuki Setyawati Soeharto, Setyawati Supranowo Supranowo Tatit Nurseta Tri Yudani Mardining Raras Utami, Juliyatin Putri Widjadjanto, Edi Yitania Sari