Tujuan penelitian ini adalah untuk mengetahui kemampuan teknologi NIRS guna memprediksi kadar lemak dan protein daging sapi aceh. Penentuan kadar protein dan lemak daging sapi aceh dilakukan pada regio Longissimus dorsi. Sampel diperoleh dari pasar Peunayong dan Lambaro, meliputi 2 sampel daging dengan masing-masing daging 3 kali pengulangan. Penelitian ini menggunakan metode Principal Component Analysis (PCA) untuk menentukan kandungan protein dan lemak daging sapi aceh Hasil penelitian ini menunjukan bahwa nilai aktual laboratorium dengan nilai prediksi NIRS memperoleh nilai yang akurat ditunjukkan dengan masing-masing R-square prediksi kadar protein dan lemak daging sapi aceh 0,99 dan 0,99 yaitu variabel prediksi terbaik. Kesimpulan dari penelitian ini adalah NIRS mampu memprediksi kadar protein dan lemak daging sapi aceh dengan sangat baik karena diperoleh nilai aktual dengan nilai prediksi dan metode NIRS dapat memprediksi kadar protein dan lemak daging sapi aceh secara akurat, karena diperoleh nilai R² = 0,99.This study aims to know the ability of NIRS technology to predicted of fat and protein content of aceh beef. Determination of protein and fat content of aceh beef was done in the Longissimus dorsi region. This research used Principal Component Analysis (PCA) method to determine protein and fat content of aceh beef. Samples were obtained from Peunayong and Lambaro markets. Sampled beef consisted of beef 2 samples with 3 repetition. The results of this study showed that the actual value of the laboratory with a predicted value of NIRS obtained an accurate value indicated by each R-square prediction protein and fat content of aceh beef aceh of 0.99 and 0.99 was the best predictive variable. It can be concluded that NIRS was able to predict the levels of protein and fat of beef as accurately. The conclusion of this study were that NIRS was able to predicted the protein and fat content of aceh beef very well because it was obtained by the actual value with prediction value and the NIRS method can predict the protein and fat content of beef aceh accurately, because the value of R² = 0,99.