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All Journal Microbiology Indonesia JERAMI Indonesian Journal of Crop Science
ISHAK MANTI
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology Medicine & Pharmacology

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Detection and Sequence Diversity of Begomovirus Associated with Yellow Leaf Curl Disease of Pepper (Capsicum annuum) in West Sumatra, Indonesia JUMSU TRISNO; SRI HENDRASTUTI HIDAYAT; TRIMURTI HABAZAR; ISHAK MANTI; . JAMSARI
Microbiology Indonesia Vol. 3 No. 2 (2009): August 2009
Publisher : Indonesian Society for microbiology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (195.352 KB) | DOI: 10.5454/mi.3.2.2

Abstract

Yellow leaf curl disease of pepper has become an emerging important disease in West Sumatra since early 2000. Several attempts have been made, including disease survey and detection, in order to identify the causal agent of the disease. Pepper (Capsicum annuum) plants showing yellow leaf curl from West Sumatra were analyzed for presence of Begomovirus employing Polymerase Chain Reaction (PCR) with degenerate primers pAL1v 1978 and pARc 715. A DNA fragment of 1.6 kb was successfully amplified and subjected to direct sequencing. A stem loop region was found in the nucleotide sequence obtained, which contains the conserved nucleotide signature sequence TAATATTAC present in begomoviruses. Based on the stem loop region comparisons and phylogenetic analysis, the virus isolates from West Sumatra showed the closest relationship to Pepper yellow leaf curl Indonesia virus (PYLVIV) and Tomato yellow leaf curl Indonesia virus (TYLCIV). The sequence was different from other Asia Begomoviruses reported earlier. These isolates were divided into three groups which were tentatively called Pepper yellow leaf curl Indonesia virus-West Sumatra-[group 1], -[group 2] and -[group 3] {PYLCIV-WS-[group1], -[group2], and -[group3]}.
The Physiological Differential Response Of Sugar Cane (Saccharum Officinaruml.) On Water Deficit Condition Renfiyeni Renfiyeni; Rhenly Danis; Dila Febria; Ishak Manti
JERAMI Indonesian Journal of Crop Science Vol 2 No 1 (2019): JIJCS
Publisher : Department of Crop Science, Faculty of Agriculture, Andalas University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (286.205 KB) | DOI: 10.25077/jijcs.2.1.1-13.2019

Abstract

Abstract Sugarcane is the main sugar-producing plant in the world and also plays an important role as a raw material for bioethanol production. Productivity improvement of the plant is exposed to environmental stress ie: water shortage which is currently a serious problem associated with the global climate change phenomenon. Understanding of plant responses to environmental stress is one of the keys to be able to resolve the issue. In this regard, the fundamental studies related to the sugarcane plant responses to water stress is very important. This study consists of a combination of two factors, namely the type of clones consisting of PS.864, PSJT.941, and VMC.76-16, 851 as tolerant group clones, PS.862, PS.882 and PS.851as non tolerant clones group and lack of water stress treatment for 5 days. The data were analyzed further using DNMRT at 5% significance level. Observations showed that tolerant clones as well as non-tolerant clones PS.862 showed better resistance response than non tolerant groups. The indication was shown by the value of the Relative Water Content (RWC), Specific leaf area (SLA) and Water Deficit Value (WDV). Total protein profiling of sugarcane grown under water deficit and its counterpart differentially distinguished by suppression of protein expression of about 35 kDa in all clones. While in the water deficit condition expression of a protein with a size of 25 kDa is remarkable expressed.
Co-Authors Dila Febria Jamsari Jamsari Jumsu Trisno Renfiyeni Renfiyeni Rhenly Danis SRI HENDRASTUTI HIDAYAT TRIMURTI HABAZAR