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All Journal Dental Journal (Majalah Kedokteran Gigi) Conservative Dentistry Journal

Devi Eka Juniarti

Department Of Conservative Dentistry Faculty Of Dental Medicine, Universitas Airlangga

Author-ID : 3196524

Dentistry

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Daya Antibakteri EkstrakMeniran (Phyllanthus niruri linn) Terhadap BakteriEnterococcus faecalis (Antibacterial Activity Of Phyllanthus niruri linn Extract Against Enterococcus faecalis Bacteria) Tri Desiana KH; Achmad Sudirman; Devi Eka Juniarti
Conservative Dentistry Journal Vol. 6 No. 2 (2016): July - December
Publisher : Universitas Airlangga

Background. Enterococcus faecalis is an anaerobic facultative gram-positive bacteria which contribute to the failure of root canal treatment with the number of prevalence 24% to 77%. At the preparation stage, a material for irrigation which has antibacterial activity to Enterococcus faecalis is needed. Phyllantus niruri linn is one of herbal medicament which is potential as antibacterial agent as it contains active antibacterial chemical-compound. Purpose. The purpose of the study is to identify Minimum Inhibitory Concentration and Minimum Bactericidal Concentrationof Phyllantus niruri linn against Enterococcus faecalis.Method. The research method used is laboratory experimental. Enterococcus faecalis ATCC 29212 was suspended into several concentration of Phyllantus niruri linn extract from dilution method on BHIB medium. Each tube was incubated for 24 hours. Then, each tube was subcultured to Nutrient agar medium using spreader in a petridish. Each petridish was incubated for 24 hours and the growth of the colony was manually calculated using CFU/ml unit. Result. At the concentration of 6.25%, Phyllanthus niruri linn was able to inhibit the growth of Enterococcus faecalis as 90% and there was no bacteria at the concentration of 12.5%. Conclusion. 6.25% concentration of Phyllanthus niruri linn extract was Minimum Inhibitory Concentration and 12.5% concentration was Minimum Bactericidal Concentration to Enterococcus faecalis.

UJI SITOTOKSISITAS EKSTRAK DAUN MIMBA (Azadirachta indica) TERHADAP SEL FIBROBLAS BHK 21 Qurni Restiani; Mandojo Rukmo; Devi Eka Juniarti
Conservative Dentistry Journal Vol. 7 No. 1 (2017): January - June
Publisher : Universitas Airlangga

Background: The leaves of neem (Azadirachta indicia) is one of herbal medicine that recommended as an alternative material of root canal irrigants. The active ingredients of neem leaves such as alkaloids, tannins, saponins and flavonoids has been proven to have antibacterial activity against E. faecalis. The ideal properties of an alternative material of root canal irrigants is not only have antibacterial activity but also is not toxic to the tissues, but the toxicity of neem leaves remains unclear until now. Objective: The aims of this study to determine the toxicity effect of neem leaves extract in specific concentration. Methods: This research was an experimental laboratory in vitro study of baby hamster kidney fibroblast (BHK-21). The neem leaves extract was made by maceration method using ethanol 96% and certain dilution performed to obtain various concentrations. Cytotoxicity test was conducted by MTT assay and the optical density was measured using ELISA reader at wavelength of 620 nm. Then, the optical density values were calculated using the formula for determining the number of survival fibroblasts after tested. Results: The percentage of survival fibroblast at concentration of 50%, 55%, 60%, 65%, 70%, 75%, 80%, 85%, 90%, 95, and 100% in sequence were 71.3%, 64, 2%, 62%, 60.2%, 40.1%, 36.3%, 36.7%, 29%, 28.4%, 27.5%, and 25.6% . Conclusion: The extract of neem leaves (Azadirachta indica) has cytotoxic effects at concentration of 70% up to 100%.

Perbedaan Daya Antibakteri Ekstrak Temulawak (Curcuma xanthorrhiza Roxb.) 3,125% dan Chlorhexidine 0,2% terhadap Lactobacillus acidophilus Nanik Zubaidah; Devi Eka Juniarti; Firza Basalamah
Conservative Dentistry Journal Vol. 8 No. 1 (2018): January - June
Publisher : Universitas Airlangga

Background: Caries is a process of demineralization of hard tissues of teeth due to the activity of bacterial metabolism. Lactobacillus acidophilus as one of the cariogenic bacteria that important process of deep caries. Efforts that can be used to inhibit cariogenic bacteria for example using antibacterial agents such as chlorhexidine that have been shown to inhibit Lactobacillus acidophilus bacteria, but chlorhexidine has some side effects. It takes basic herbal ingredients as an alternative agent that can inhibit the growth of cariogenic microorganisms, one of them is temulawak extract. Purpose: This research was conducted to compare antibacterial agent between Curcuma xanthorrhizaRoxb and chlorhexidine 0,2% to inhibit Lactobacillus acidophilus. Methods: The research was a laboratory experimental study. Temulawak extract was made by maceration method with etanol 96% and certain dilution was performed to obtain concentration of temulawak extract 100%, 50%, 25%, 12,5%, 6,25%, 3,125%, 1,56%, 0,781%. A preliminary study was conducted to determine the value of KHM and KBM of temulawak extract against Lactobacillus acidophilus were known by counting the growth of bacteria colonies on muellerhinton media in CFU/ml. From the calculation of the number of colonies Lactobacillus acidophilus, obtained KHM value of 3.125% and KBM value of 6.25%. Further research is conducted by comparing antibacterial ability of temulawak extract used KHM concentration and chlorhexidine 0,2% by using diffusion method by measurement of inhibiton zoneResult: Measurement result of inhibition zone by using calipers show that chlorhexidine 0,2% have inhibiting zone (in mm) 15.1; 15.2; 15.4; 14.9; 15.0; 15,15; 15.5; 15.7; 15.8; 16.0; 14.6; 15.2; 14,85; 15,1; 15.3; 15,2, while the measurement of inhibition zone of temulawak extract 3,125% are (in mm) 10,0; 10.3; 10.2; 9.9; 9.8; 10.1; 10.3; 10.1; 10.45; 10.1; 9.95; 9.85; 8.85; 9.35; 9.45; 9.4. Conclusion: Chlorhexidine 0,2% more potential as antibacterial agent than temulawak extract 3.125% to inhibit Lactobacillus acidophilus.

Effect Of Epigallocatechin-3-Gallate (EGCG) On The Number Of Macrophage Cells In Inflammation Of Pulp With Mechanical Injury Kun Ismiyatin; Devi Eka Juniarti; Djoko Agus Purwanto; Adecha Ekalipta Primazafira
Conservative Dentistry Journal Vol. 10 No. 1 (2020): January-June
Publisher : Universitas Airlangga

Background: Pulpitis is an inflammatory pulp that can occur due to mechanical trauma that causes perforation of the pulp. Treatment of pulpitis Emergency frequently using Eugenol. High concentrations of Eugenol have a cytotoxic effect that causes local necrosis and inhibits the recovery process. An alternative consideration with Epigallocatechin 3-gallate (EGCG) which has good antioxidant properties and increases the complexity of inflammation by inhibiting the production of inducible nitric oxide synthase (iNOS) and nitric oxide so that levels migration of inflammatory cells to the area of injury will decrease and inflammation will occur shorter then initiates the proliferation so the recovery process and tissue repair will be more rapidly occurring. One of the cells that support tissue repair is macrophages. Purpose: this study aims to explain the effect of EGCG on the number of macrophage cells in pulp inflammation with mechanical injury. Methods: The study used 24 Wistar rats teeth divided into four groups, namely control (C), and 3 treatment groups T1, T2, and T3. Each group consisted of 6 rats prepared, then T1 was named EGCG hydrogel 60 ppm, T2 was given EGCG hydrogel 90 ppm, while T3 was given EGCG hydrogel 120 ppm. On the 3rd day, Wistar rats were decapitated to continue making HPA preparations. Results:This study showed a significant difference in each group (p< 0.05) using One-Way Anova analysis. Conclusion: EGCG hydrogel 90 ppm is effectivein increasing the number of macrophage cells.

THE EFFECT OF CALCIUM HYDROXIDE-PROPOLIS ON THE NUMBER OF MACROFAG CELLS ON THE WISTAR RAT DENTAL WITH PULPA PERFORMANCE Setyabudi Setyabudi; Devi Eka Juniarti; Ira Widjiastuti; Maughfirah Shintya Fathori
Conservative Dentistry Journal Vol. 10 No. 2 (2020): July-December
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/cdj.v10i2.2020.58-61

Background: Cellular response of dental pulp to existing lesions, caused by infiltration of inflammatory cells that migrate from blood vessels to the site of injury. Macrophages are the cells that most appear when inflammation occurs. Treatment for maintaining pulp tissue vitality is called pulp capping. Calcium hydroxide as a pulp capping material has the ability to trigger the growth of dentin bridges or remineralization, but calcium hydroxide can induce migration and proliferation of inflammatory cells. Currently the field of dentistry is developing propolis as an alternative pulp capping material. That is because propolis has anti-inflammatory properties. Objective: To analyze the effect of the combination of calcium hydroxide-propolis on the number of macrophage cells in the teeth of wistar rats with pulp perforation. Method: This study used 30 samples of Wistar rats which were preparated until perforation and then divided into 3 groups. The first group continued with filling with cention, the second group continued with application of calcium hydroxide and filled with cention, and the third group continued with the application of combination of calcium hydroxide and propolis and then being filledwithcention. Macrophage cell is calculated histopathologically by using compound light microscope on the 3rd day with 400x magnification. Results: The Kruskal-Wallis test results showed significant differences in macrophages after the combination of calcium hydroxide and propolis (p = 0,000). The combined application of calcium hydroxide and propolis showed higher macrophages than the application of calcium hydroxide and the control group. Conclusion: Application of calcium hydroxide - propolis combination was proven increase the number of macrophage cells in Wistar Rats (Rattus Norvegicus) with pulp perforation

Complex Aesthetic Treatment of Patients with Multiple Caries Devi Eka Juniarti; Tamara Yuanita; Nirawati Pribadi; Tarsardo Marbun; Nadien Sekar Pramesti
Conservative Dentistry Journal Vol. 12 No. 1 (2022): January-June
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/cdj.v12i1.2022.38-44

Background: The need for dental esthetics is very important in recent years. Fulfilling aesthetic demands with both non-invasive and minimally invasive techniques can maintain tissue continuity. A 38 year old female patient came to the clinic with an esthetic complaint of maxillary anterior teeth. The following case report describes a step by step complex aesthetic treatment of a patient with multiple caries. Appealing aesthetic results will restore the patient's smile through restorative treatment in dentistry. Purpose: The purpose of this case report is to explain the management of complex aesthetic treatment of patients with multiple caries Case: A 38-year-old female patient complained her upper front teeth is turning black. The patient complained that her teeth had hurt ± 3 months ago and sometimes felt sore. The patient wants her teeth to be treated because she feels less confident when she smiles and wants to fix these teeth. The history of treatment on the tooth in question have had root canal treatment and been filled since 8 years ago. The tooth has had braces (orthodontic) treatment and was removed ± 7 years ago. The patient stated that she had no systemic health disorder. Case management : The treatment of this complex case were done using root canal treatment, endodontic retreatment, direct and indirect veneer, dowel, and crown. Conclusion: complex aesthetic treatment of patients with multiple were done aiming for good aesthetic result

Treatment of multiple diastemas in maxillary anterior teeth with indirect veneers: A case report Devi Eka Juniarti; Satria Aji Prasidha; Rhegina Zada Aramita Putri; Yulianti Kartini Sunur
Conservative Dentistry Journal Vol. 12 No. 2 (2022): July-December
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/cdj.v12i2.2022.77-81

Background: Multiple diastema is one of the most frequently encountered aesthetic problems related to patient confidence. This problem is still a challenge for the clinician to restore the gap between the teeth as desired by the patient. Indirect veneers have been approved as one of the treatment plans to deal with this aesthetic problem. Purpose: This case report aims to describe the management of multiple diastema in maxillary anterior teeth with indirect veneer restorations. Case: A 20-year-old woman came to RSKGM Universitas Airlangga with the main complaint of a gap between her upper front teeth. The patient was then explained about all the treatment options and agreed to a treatment plan for the indirect veneers restoration. Case Management: Treatment was carried out in 5 visits involving 6 maxillary anterior teeth. The teeth were vital and there was no discoloration. Post-treatment control was carried out one week after insertion. One of the advantages of bonded porcelain veneers is the minimally invasive treatment. Only 0.5 mm reduction was required on the incisal and labial surfaces. Conclusion: Indirect veneer restoration with minimally invasive techniques can provide good results in treating multiple diastema in the maxilla.

Effective Dose of Nano Propolis as Anti-Pain in Animal Models of Mus Musculus Using Writhing Test Method Annisa Salsabila Witjaksana; Ira Widjiastuti; Devi Eka Juniarti
Conservative Dentistry Journal Vol. 13 No. 1 (2023): January-June
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/cdj.v13i1.2023.7-10

Background: Odontogenic pain has a high prevalence where this pain is the result of noxious physical stimuli or calming inflammatory mediators that stimulate receptors located at the terminal ends of nociceptive C and AÎ´ afferent nerve fibers. Nano propolis is an alternative material that is used as a medicine in dentistry. Nano propolis is a mixture of natural resins containing flavonoids and phenolic acids which play a role in suppressing pain response through COX and NF-kB inhibition mechanisms. Nanoparticle technology is capable of preparing active drug ingredients in nano-sized particles and can affect drug effectiveness, because particle size affects the process of solubility, absorption and distribution of drugs. Research on the potential of nano propolis as an anti-pain is still minimal, so further research is needed on the function of nano propolis as an alternative anti-pain agent. Purpose: To determine the effective dose of nano propolis on pain response in Mus musculus. Methods: This research was conducted using the writhing test method to see the analgesic effect of nano propolis. The research sample consisted of 28 Mus musculus which were divided into four treatment groups, namely the piroxicam control group, and the nano propolis treatment group of 17.5 mg/kg, 35 mg/kg, and 70 mg/kg. Results: The results showed that the four treatment groups did not have a significant difference, in other words, they had the same anti-pain effect. Conclusion: Nano propolis 35 mg/kgBB is an effective dose in suppressing pain response in Mus musculus.

Inhibitory effect of nano Stolephorus insularis and calcium hydroxide on glucosyltransferase (GTF) activity of Lactobacillus aciophilus Tamara Yuanita; Devi Eka Juniarti; Tarsardo Marbun
Conservative Dentistry Journal Vol. 13 No. 1 (2023): January-June
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/cdj.v13i1.2023.29-32

Background: Dental caries is the most common multifactorial infectious disease worldwide which referes to the process of destruction tooth hard tissue caused by bacterial by-products in the form of acids through carbohydrate fermentation. Lactobacillus acidophilus is one of the main cariogenic bacteria that causes caries by forming biofilms with the help ofglucosyltransferase (GTF) enzymes. Calcium hydroxide has limited antibacterial effects. Nano brown anchovy contains fluor as the active compound which has the potential as an antibacterial alternative agent. Purpose: To explain the inhibition of the combination of nano Stolephorus insularis and calcium hydroxide on the activity of the glucosyltransferase enzyme of Lactobacillus acidophilus bacteria. Methods: This study used a combination of nano Stolephorus insularis 3.125% with calcium hydroxide as a treatment and aquades as a control. The GTF enzyme was obtained from the supernatant centrifuged by Lactobacillus acidophilus in BHIB. The activity of the GTF enzyme was considered by calculating the fructose levels using High Performance Liquid Chromatography (HPLC) in a certain formula.Results: Decreased levels of fructose was obtained in the treatment group. From the results of Mann- Whitney data analysis, there were significant difference in the study groups. Conclusion: the combination of nano Stolephorus insularis with calcium hydroxide is effective to inhibit the glucosyltransferase enzyme activity of Lactobacillus acidophilus bacteria.

Co-Authors Achmad Sudirman Achmad Sudirman Adecha Ekalipta Primazafira Adioro Soetojo Ananta Tantri Budi Annisa Salsabila Witjaksana Dian Agustin Wahjuningrum Djoko Agus Purwanto Dyanita Hasri Eresha Melati Kusuma Wurdani Eric Priyo Prasetyo Evelyn Tjendronegoro Firza Basalamah Galih Sampoerno Ira Widjiastuti Ira Widjiastuti Karlina Samadi Kun Ismiyatin Mandojo Rukmo Maughfirah Shintya Fathori Nadien Sekar Pramesti Nanik Zubaidah Nirawati Pribadi Qurni Restiani Rhegina Zada Aramita Putri Satria Aji Prasidha Setyabudi Setyabudi Tamara Yuanita Tarsardo Marbun Tri Desiana KH Yulianti Kartini Sunur

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