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Peranan sorbitol dalam mempertahankan kestabilan pH saliva pada proses pencegahan karies (The role of sorbitol in maintaining saliva’s pH to prevent caries process) Diana Soesilo; Rinna Erlyawati Santoso; Indeswati Diyatri
Dental Journal (Majalah Kedokteran Gigi) Vol. 38 No. 1 (2005): March 2005
Publisher : Faculty of Dental Medicine, Universitas Airlangga https://fkg.unair.ac.id/en

People in Indonesia often consume food containing sucrose. If the sucrose consumed is in a large amount, it will decrease saliva’s pH and soon teeth destruction will happen. To avoid it, it is necessary to change sucrose consumption habit into another kind of sugar, namely sorbitol. Sorbitol is preferred to use, because it is cheaper and easier to get. Sorbitol is made from cassava, which is plentifully grown in Indonesia. Sorbitol is not good media for bacteria to grow. Because sorbitol has a diol, so it’s difficult to catalyst by glucosyltransferase enzyme, which is produced by bacteria Streptococcus mutans. The conclusion is that sorbitol is difficult to be fermented by Streptococcus mutans so it will not decrease saliva’s pH.

Correlation between working positions and lactic acid levels with musculoskeletal complaints among dentists Fiory Dioptis Putriwijaya; Titiek Berniyanti; Indeswati Diyatri
Dental Journal (Majalah Kedokteran Gigi) Vol. 49 No. 4 (2016): December 2016
Publisher : Faculty of Dental Medicine, Universitas Airlangga https://fkg.unair.ac.id/en

Background: Musculoskeletal complaints have been common for dentists since their body is unknowingly often in inappropriate positions when caring for patients. For example, they bend towards patients, suddenly move, and then rotate from one side to another. The repetitive movements are done in long term. High activities and sufficient recovery time can cause a buildup of lactic acid in their blood leading to obstruction of the energy intake from the aerobic system in their muscle cells, resulting in fatigue. As a result, such conditions trigger decreased muscle performances. Purpose: This study aimed to determine the correlation between working positions and lactic acid levels with the risk of musculoskeletal disorders among dentists at Public Health Centers in Surabaya. Method: This research was an analytical observational research using cross sectional approach. Sampling technique used in this research was cluster random sampling with nineteen samples. To evaluate the working positions of those samples, a rapid entire body assessment (REBA) method was used. Meanwhile, to observe the musculoskeletal disorders of those samples, a Nordic body map was used. Data obtained then were analyzed using Pearson correlation test with a significance level (p<0.05). Result: Results of data analysis using the Pearson correlation test showed that the significance value obtained was 0.036. It indicates that there was a correlation between the working positions and the lactic acid levels with the musculoskeletal disorders in those dentists. The results of the Pearson correlation test also revealed that there was a correlation between the working positions and the lactic acid levels among those dentists with a significance value of 0.025. Conclusion: It may be concluded that the wrong body positions during working can increase lactic acid level in the body of dentists. The increased level of lactic acid then can affect their muscles, leading to the high risk of musculoskeletal disorders.

The potency of Immunoglobulin Y anti Porphyromonas gingivalis to inhibit the adherence ability of Porphyromonas gingivalis on enterocytes Nova Andriani Hepitaria; Indeswati Diyatri; Markus Budi Rahardjo; Rini Devijanti Ridwan
Dental Journal (Majalah Kedokteran Gigi) Vol. 53 No. 1 (2020): March 2020
Publisher : Faculty of Dental Medicine, Universitas Airlangga https://fkg.unair.ac.id/en

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/j.djmkg.v53.i1.p20-23

Background: Pophyromonas gingivalis (P. gingivalis) bacteria are the main type of bacterium that cause chronic periodontitis. Immunoglobulin Y (IgY) is a type of immunoglobulin found in poultry, such as chickens and birds. IgY can be used as an alternative method of preventing the accumulation of plaque that causes chronic periodontitis. Purpose: To determine the ability of IgY anti P. gingivalis to inhibit adherence of P. gingivalis. Methods: The samples were divided into eight groups, each group containing 10 ml of IgY anti P. gingivalis and 50 ml of enterocyte cells. The control group contained 50 ml of IgY anti P. gingivalis, and 50 ml of enterocyte cells. Serial dilution was carried out to the first seven groups, with the first group containing 90 ml phosphate-buffered saline (PBS) and 10 ml IgY anti P. gingivalis, and the second to seventh groups containing 50 ml PBS before adding 50 ml of enterocyte cells and 50 ml of bacterial suspension per group. The number of bacteria was calculated as an adherence index value using a light microscope. Results: This study shows that IgY anti P. gingivalis significantly reduces the adherence index value of P. gingivalis. Conclusion: IgY anti P. gingivalis has potency to inhibit the adherence of P. gingivalis.

Density of Streptococcus mutans biofilm protein induced by glucose, lactose, soy protein and iron Indah Listiana Kriswandini; Indeswati Diyatri; Intan Amalia Putri
Dental Journal (Majalah Kedokteran Gigi) Vol. 52 No. 2 (2019): June 2019
Publisher : Faculty of Dental Medicine, Universitas Airlangga https://fkg.unair.ac.id/en

Background: Caries constitute an infectious disease that result from the interaction of bacteria with the host and the oral environment. Streptococcus mutans (S. mutans) represents the main bacterium that causes caries. The ability of S. mutans to form biofilms in the oral cavity is influenced by daily nutrient intake. This study of bacterial biofilm proteins can be used in the manufacture of kits for the detection of infectious diseases such as caries in the oral cavity. A biomarker is required for the manufacture of the detection kit. Consequently, research must first be conducted to determine the molecular weight and density of S. mutans biofilm proteins induced by several different daily nutrients, namely; 5% glucose, 5% lactose, soy protein and 5% iron. Purpose: This study aimed to analyse the density of S. mutans biofilm protein induced by 5% glucose, 5% lactose, soy protein, and 5% iron. Methods: The density of the S. mutans biofilm protein bands induced were measured using EZ Imager Gel DocTM software. Results: A band of biofilm protein (61.7 kDa) was obtained from S. mutans induced by 5% glucose, four bands of biofilm protein (180 kDa; 153,9 kDa; 43,9 kDa; 37,5 kDa) from 5% lactose induction and seven bands of biofilm protein (157,9 kDa; 86,6 kDa; 66,5 kDa; 50,1 kDa; 37,9 kDa; 32,3 kDa; 29,4 kDa) from soy protein induction. In contrast, S. mutans induced by 5% iron did not show any protein bands. The proteins that result from each inducer are of differing densities. Conclusion: The protein bands from each inducer are of different densities which can be used in the further test to make a biomarker for dental caries detection kits.

The effect of X-ray irradiation to the formation of polychromatic erythrocyte cell micronucleus in Wistar rats (Rattus norvegicus) Eha Renwi Astuti; Hutojo Djajakusuma; Indeswati Diyatri; Nastiti Faradilla Ramadhani
Dental Journal (Majalah Kedokteran Gigi) Vol. 54 No. 3 (2021): September 2021
Publisher : Faculty of Dental Medicine, Universitas Airlangga https://fkg.unair.ac.id/en

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/j.djmkg.v54.i3.p128-131

Background: Panoramic and cephalometric radiography is very important for diagnosis, treatment plan, and evaluation of orthodontic treatment results. Panoramic and cephalometric radiography are frequently performed at the same time, causing DNA damage and chromosome aberration. Purpose: This study aims to analyse the effect of X-ray exposure in panoramic and cephalometric radiography on micronuclei cell numbers. Methods: Laboratory-based analytical study with 60 healthy-male Wistar rats weighing 200–300 grams divided into 6 treatment groups (n=10). The control group: without radiographic exposure, the treatment group 2: using panoramic radiographic exposure followed by cephalometric, and the treatment group 3: using panoramic radiographic exposure and 24 hours later performed cephalometric radiographic. The unit of analysis was the polychromatic erythrocytes of mice cell, were examined 24 hours and 48 hours after irradiation had been finished. The polychromatic erythrocytes were examined using May-Gruenwald-Giemsa staining and 100x magnification under a microscope with 2000 cells per view. Data obtained were analysed using the SPSS 20 version software. The mean and standard deviations were calculated for each clinical parameter, and a one‐way ANOVA statistical test of significance was used. Statistical significance was set at p<0.05. Results: The analysis showed a significant increase (p<0.05) in the number of micronucleus in groups that used panoramic radiographic exposure followed by cephalometric. Conclusion: X-ray radiation can increase the number of micronucleus in polychromatic erythrocyte cells in rats.

A mucoadhesive gingival patch with Epigallocatechin-3-gallate green tea (Camellia sinensis) as an alternative adjunct therapy for periodontal disease: A narrative review Yeka Ramadhani; Riski Rahayu Putri Rahmasari; Kinanti Nasywa Prajnasari; Moh. Malik Alhakim; Mohammed Aljunaid; Hesham Mohammed Al-Sharani; T. Tantiana; Wisnu Setyari Juliastuti; Rini Devijanti Ridwan; Indeswati Diyatri
Dental Journal (Majalah Kedokteran Gigi) Vol. 55 No. 2 (2022): June 2022
Publisher : Faculty of Dental Medicine, Universitas Airlangga https://fkg.unair.ac.id/en

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/j.djmkg.v55.i2.p114-119

Background: Periodontitis is a progressive destructive periodontal disease. The prevalence of periodontal disease in Indonesia reaches 74.1% and mostly occurs in the productive age group. Most of the periodontopathogenic bacteria are gram-negative bacteria and have endotoxin in the form of lipopolysaccharide (LPS), which can penetrate the periodontal tissue and induce an inflammatory response. In inflammatory conditions, osteoclastic activity is higher than osteoblastic activity, which causes bone destruction. This results in an imbalance between osteoclast-induced bone resorption and osteoblast-induced bone formation. The current preferred treatment for periodontitis is scaling root planning (SRP), but this therapy cannot repair the damaged periodontal tissue caused by periodontitis. Purpose: To describe the possibility of using a mucoadhesive gingival patch with Epigallocatechin-3-gallate (EGCG) green tea (Camellia sinensis) as alternative adjunct therapy for periodontal disease. Review: EGCG is the main component of green tea catechins, which have antitumor, antioxidant, anti-inflammatory, anti-fibrotic, and pro-osteogenic effects. However, the weaknesses so far regarding the use of EGCG as an alternative treatment is its low oral bioavailability and the concentration of EGCG absorbed by the body decreasing when accompanied by food. EGCG can be used with a mucoadhesive gingival patch to optimise bioavailability and absorption and increase local concentration and sustained release of EGCG. EGCG encourages bone development and braces mesenchymal stem cells (MSCs) differentiation for osteoblast by enhancing the expression of bone morphogenic protein 2 (BMP2). EGCG also has been proven to increase the expression of RUNX2 and ALP activity that induces osteoblast differentiation and bone mineralisation. Conclusion: A mucoadhesive gingival patch containing EGCG Green Tea (C. sinensis) may potentially induce osteoblastic activity as an adjunct therapy to repair the periodontal tissue damage due to periodontal disease.

INOVASI PEMBUATAN HAND SANITIZER UNTUK MENINGKATKAN PERILAKU HIDUP BERSIH DAN SEHAT BAGI KOMUNITAS DI BONDOWOSO Rini Devijanti Ridwan; Tuti Kusumaningsih; Indeswati Diyatri; Sidarningsih Sidarningsih
Jurnal Layanan Masyarakat (Journal of Public Services) Vol. 5 No. 2 (2021): JURNAL LAYANAN MASYARAKAT
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jlm.v5i2.2021.287-291

AbstractClean and Healthy Living Behaviors as known as PHBS are all health behaviors that are carried out with awareness, so that family members or families can help themselves in the health sector and play an active role in health activities in the community. One of the activities that can be carried out to achieve PHBS is washing hands using soap (hand rub / hand wash) or cleaning fluid (hand sanitizer). About 98% of the spread of microorganisms in the body comes from our own hands. Maintaining hand hygiene is an effort to avoid various diseases. The benefits of washing hands are very large, washing hands using soap / hand rub or using a hand sanitizer only takes 20 seconds but is very useful for killing microorganisms, namely bacteria, fungi, and viruses. One way to combat the new corona virus or COVID-19, which has become a pandemic at this time, is to always maintain hand hygiene. With the pandemic, the price of hand sanitizers has skyrocketed because the need has increased sharply. When used too often, alcohol as the main ingredient of hand sanitizers, besides being able to irritate the skin, it can also lift natural oils on the hands and tend to be drier, so herbal ingredients are needed as natural ingredients to reduce the side effects of these hand sanitizers. This community service activity was carried out in collaboration with communities in the Bondowoso district, namely the KAPAS Community in Sumbergading Village, Sumberwringin District and the TSS Community in Sukosari Lor Village, Sukosari District. In the Bondowoso area, there are many youth communities with various activities aimed at advancing people's lives and improving the socio-economy in the region. Various plants can be found in the area, including aloe vera and betel leaf.Keywords : Health, hand wash, hand sanitizer, aloe vera, betel leafAbstrakPerilaku Hidup Bersih dan Sehat (PHBS) merupakan semua perilaku kesehatan yang dilakukan atas kesadaran, sehingga anggota keluarga atau keluarga dapat menolong dirinya sendiri di bidang kesehatan dan berperan aktif dalam kegiatan kesehatan di masyarakat. Salah satu kegiatan yang dapat dilakukan guna tercapainya PHBS adalah mencuci tangan menggunakan sabun (hand rub/hand wash) atau cairan pembersih (hand sanitaizer). Sekitar 98% penyebaran mikroorganisme di tubuh bersumber dari tangan kita sendiri. Menjaga kebersihan tangan salah satu upaya terhindar dari berbagai penyakit. Manfaat mencuci tangan sangat besar, mencuci tangan memakai sabun/hand rub atau menggunakan hand sanitizer hanya membutuhkan waktu 20 detik namun sangat berguna untuk membunuh mikroorganisme, yaitu bakteri, jamur dan virus. Salah satu cara untuk memerangi virus korona baru atau COVID-19 yang telah menjadi pandemi saat ini adalah dengan selalu menjaga kebersihan tangan. Masa pandemi ini mengakibatkan harga hand sanitizer melambung tinggi karena kebutuhan meningkat tajam. Alkohol sebagai bahan utama dari hand sanitizer bila terlalu sering digunakan, selain dapat membuat kulit iritasi juga dapat mengangkat minyak alami pada tangan dan cenderung jadi lebih kering, sehingga diperlukan bahan herbal sebagai bahan alami untuk mengurangi efek samping dari hand sanitizer tersebut. Pada kegiatan pengmas ini dilakukan kerjasama dengan komunitas di wilayah kabupaten Bondowoso yaitu Komunitas KAPAS di Desa Sumbergading Kecamatan Sumberwringin dan komunitas TSS Desa Sukosari Lor Kecamatan Sukosari. Di wilayah Bondowoso banyak didapatkan komunitas anak muda dengan beragam kegiatan yang bertujuan memajukan kehidupan masyarakat dan meningkatkan sosial ekonomi di wilayah tersebut. Beragam tanaman dapat dijumpai di wilayah tersebut, diantaranya aloe vera dan sirih.Kata kunci: Kesehatan, cuci tangan, hand sanitizer, aloe vera, daun sirih

LITERACY CHARACTERISTICS OF CALCULUS AND ITS ROBLEMS IN THE CONTEXT OF COMMUNITY SERVICE Indeswati Diyatri; Keyona Laila Olivia; Mahela Sefrian Luthfimaidah; Dimas Rudianto Fardha
Jurnal Layanan Masyarakat (Journal of Public Services) Vol. 6 No. 1 (2022): JURNAL LAYANAN MASYARAKAT
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jlm.v6i1.2022.143-145

The prevalence of dental and oral diseases in Indonesia tends to increase. The problem of high rate in dental and oral disease are currently influenced by community behavioural factors. High rate of dental and oral disease was also because of the Indonesian population who realized that they has dental and oral problem is only 23% of population. Community behaviour is closely related to the level of public knowledge about gum health and tartar. Lack of knowledge about gum health and ignorance of the dangers of tartar that caused by dental plaque causing people not to take advantage of dental health services when they have dental plaque and even become inflammation of the gums. That condition, will contribute to poor dental health status of the community. Therefore, this community service is carried out for educate the characteristics of calculus, gum inflammation and the treatment of tartar.

INNOVATION FOR MANUFACTURING FERTILIZER FROM COFFEE LEATHER WASTE FOR THE COMMUNITY AND FARMER GROUP IN BONDOWOSO Rini Devijanti Ridwan; Indeswati Diyatri; Sidarningsih; Yuliati
Jurnal Layanan Masyarakat (Journal of Public Services) Vol. 6 No. 1 (2022): JURNAL LAYANAN MASYARAKAT
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jlm.v6i1.2022.212-218

The coffee husk waste has not been utilized optimally by farmers. Coffee skin waste, which has been considered as a leftover material from the production of ground coffee, turns out to have many benefits and uses in life. Based on the results of research by experts, coffee husk waste is useful in agriculture, animal husbandry and fisheries, namely as compost, protein nutrition and additional fiber in animal feed. This solid waste of coffee rind has high levels of organic matter and nutrients that can improve soil structure. One of the efforts that can be done to handle the increasing amount of coffee husk waste is by processing coffee skin waste into compost. The by-products in the form of coffee skins range from 50-60 percent of the harvest. If the yield is 1000 kg of fresh coffee, then about 400-500 kg of coffee beans will be produced and the rest is a by-product in the form of coffee husks. The advantage of this coffee skin compost is that its nitrogen content is quite high, around 6% so that it can substitute for fertilizers containing nitrogen. The problem is that the C/N ratio is high enough that it takes a long time to decompose or the solution is to add nitrogen sources and a decomposer to speed up decomposition. This community service activity was carried out in collaboration with the community in the Bondowoso district, namely the KAPAS Community in Sumbergading Village, Sumberwringin District and the TSS community in Sukosari Lor Village, Sukosari District. In the Bondowoso area, there are many youth communities with various activities aimed at advancing people's lives and improving the socio-economic status of the area. Coffee husk waste is very common in the area, so far, coffee skin waste has not been used optimally, so its benefits cannot be obtained. The solutions offered in this community service activity include: Provide an understanding of the importance of utilizing existing coffee husk waste for communities and communities in the Bondowoso area, Provide training on making fertilizer from coffee husk waste as an innovation in making fertilizer to increase agricultural yields in the Bondowoso area, Exploring and empowering the natural potential that exists in the community environment so that it can be put to good use, Initiate the business of making fertilizer from coffee husk waste as an innovation in making fertilizer for cost efficiency in agriculture Keywords : waste, coffee husk, innovation, fertilizer, health DAFTAR PUSTAKA Dzung NA., Dzung TT.,Khanh VTP.,2013. Evaluation of Coffe Husk Compost for Improving Soil Fertility and Sustainable Coffee Production in Rural Central Highland of Vietnam. Resources and Environment, 3(4), 77-82 Abdoellah, S dan A.Wardani. 1993. Impact of Cocoa Development on Marginal Land to Farmers Income: A Case in Gunung Kidul Regency, Indonesia. Pelita Perkebunan, 9(3), 97 – 104 Bressani, R.1979. The by-products of coffee berries.dalam coffee pulp: composition, technology, and utilization. Editor J. E. Braham and R. Bressani. Ottawa: Institute of Nutrition of Central America and Panama. Berlian, Z., Syarifah, dan Sari DS., 2015. Pengaruh Pemberian Limbah Kulit Kopi (Coffea robusta L.) terhadap Pertumbuhan Cabai Keriting (Capsicum annum L.). Jurnal Biota, 1(1), 22-32. Sahputra, A., Barus A., dan Sipayung R., 2013. Pertumbuhan dan produksi bawang merah (Allium ascalonicum L.) terhadap pemberian kompos kulit kopi dan pupuk organik cair. Jurnal Online Agroekoteknologi, 2(1), 26-35. Maruli, A. 2010. Limbah kopi antar mahasiswa ke jerman. http://www.antaranews.com/berita/2 27334/limbah-kopi-antarmahasiswake jerman/2017.07.18. Afrizon, 2015. Potensi Kopi Sebagai bahan Baku Pupuk Kompos Di Propinsi Bengkulu. AGRITEPA, 2(1). 1, 21-32 Novita E., Fathurrohman A., Pradana HA.,2018. Pemanfaatan Kompos Blok Limbah Kulit Kopi sebagai Media Tanam. Jurnal Agrotek, 2(2), 61- 72 Muryanto, 2004 Muryanto. (2004). Potensi Limbah Kulit Kopi Sebagai Pakan Ternak. Jurnal Lokakarya, 1, 112-114. Triawan DA., Banon C., Adfa M., 2020. Biokonversi Kulit Kopi Menjadi Pupuk Kompos Pada Kelompok Tani Pangestu Rakyat Kabupaten Rejang Lebong. Jurnal Pengabdian Al-Ikhlas, 5(2), 159-165

Differences Post-extraction Collagen-density of Wistar-rat With Aerobic and Anaerobic Interval-training Aqsa Sjuhada Oki; Indeswati Diyatri; Kadek Gede Putra Wibawa
Indonesian Journal of Dental Medicine Vol. 2 No. 2 (2019): July - December
Publisher : Faculty of Dental Medicine Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/ijdm.v2i2.2019.32-34

Background: Tooth extraction is extracting teeth from alveolar bone. In the world, tooth loss is a disease that affects the function and aesthetics oral cavity, it causes 7.5 million Disability-Adjusted Life Year (DALY). Tooth extraction rate in Indonesia is around 24% in 2013 and 19% in 2018. Sports is one of the activities that can help accelerate wound healing after tooth extraction, this study examine differences in collagen density after tooth extraction of Wistar rats given aerobic and anaerobic interval training. Objective: To prove differences in post-extraction collagen density of Wistar Rat (Rattus norvegicus) with aerobic and anaerobic interval training. Method: Wistar rats were divided into 3 groups, control group (K) rat were soaked in a bucket with tap water as high as the rat soles with a duration of 50% of maximum swimming ability (KRM), aerobic interval (P1) 3% load swim test with duration of 3 sets 50 % KRM exercise and 25% KRM rest, anaerobic interval P2 load 6% duration 80% KRM exercise and rest with 4 sets. All groups were observed on the 3rd and 7th day post extraction. Data were analyzed using Kruskal-Wallis and continued with Post-hoc test to test differences between groups. Results: There was only significant difference in collagen thickness between groups K(3) and K7, and another groups had not significant difference. Conclusion: there unsignificant differences in collagen density after tooth extraction in Wistar rats (Rattus norvegicus) with aerobic and anaerobic interval training.

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