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Effect of short one-third lower face toward mastication performance in Deutero Malay Dian Anggraeni; Roosje Rosita Oewen; Jakobus Runkat; Syarief Hidayat
Padjadjaran Journal of Dentistry Vol 26, No 3 (2014): November 2014
Publisher : Faculty of Dentistry Universitas Padjadjaran

The one-third lower face height belongs to ideal proportion, ratio 1:1:1, of vertical face height. The one-third lower face height could be influenced by mastication. The one-third lower face height that shorter than normal ratio is generally followed by deep bite which may lead to temporomandibular disfunction even so eustachian tube disfunction, this condition may interfere mastication function, which can be measured by mastication performance. The research objective was to determine the effects of short lower face height to the mastication performance based on overbite. The research method was a cross-sectional study with epidemiology survey type. Research subjects 12-15 years old junior high school with Deutero Melayu Sub races in Bandung. Sampling technique used multistage random sampling by determining the size of the sample based on potluck sample and gained 24 children (group 1) with the normal ratio of vertical face height and 27 children with short lower facial height which were 11 children overbite normal (group 2) and 16 children deep bite (group 3). Mastication performance by 20 times chewing artificial test food then followed by sieving test. Mastication performance value represented by the median particle size (MPS) and the distribution of the particle distribution (b). The results of average MPS and b between group 1, 2 and 3 used statistical analysis MPS with ANOVA test which Fhit=5.56 and pvalue=0.0075, b which Fhit=3.41 and pvalue=0.0430 showed significant differences. Continued with group T test MPS (value = 0.0925) dan b (value = 0.2076) between group 1 and 2 showed a non significant differences. While between group 1 and 3 showed a highly significant differences in MPS (value = 0.0037 and  = 0,01) and a significant differences in b (value = 0.0141 and  = 0,05). Conclusions of this study were children with short lower face height and normal overbite did not decrease the mastication performance while children with deep bite decrease the mastication performance.

Differences in dental age of small gestational age children based on the severity of enamel dental defects Annisa Maya Kania; Roosje Rosita Oewen; Williyanti Suwondo
Padjadjaran Journal of Dentistry Vol 22, No 2 (2010): July 2010
Publisher : Faculty of Dentistry Universitas Padjadjaran

Small Gestational Age (SGA) is the term used for a new born baby with birth-severe below the 10th percentile on the intrauterine Lubchenco curve, due to Intra Uterine Growth Restriction (IUGR) which can cause defects of several organs such as deciduous teeth enamel. Dental age is the indicator for growth and development of the teeth that determine biological age which is more accurate than chronological age and could be determined by calcification or teeth eruption. The purpose of this study was to obtain dental age in SGA children based on the enamel defect severity level of deciduous teeth as compared to the chronological age. This study was a comparative analytic study with given sample size, conducted towards 18 SGA children aged 4-6 years old, and 21 children with Appropriate Gestational Age (AGA) aged 4-6 years old as the control group. Deciduous teeth enamel defect severity level determined by the developmental defect of enamel (DDE) score FDI modified. In order to determine the permanent teeth growth and development, the indicator was based on dental age using the panoramic radiograph with Dermijian method. The t-test was used to compare the permanent teeth dental age difference between SGA and AGA children as well as the permanent teeth dental age difference of SGA children based on the enamel defect severity level of deciduous teeth as seen in their chronological age. The result showed that there was a difference in the permanent teeth dental age between the SGA and AGA children at chronological ages of 4, ,5, and 6 years old (t count = 3,36; 2,35; and 3,49). Based on the average of permanent teeth dental age, the value of AGA children was higher than SGA children. Furthermore, it also showed that the severe score of EDS in the SGA children at the age of 4, 5, and 6 years old, their permanent teeth dental age was lower than the mild EDS (t count = 1,45). The conclusion of this study was the permanent teeth dental age in SGA children was lower than the AGA children as well as difference between the permanent teeth dental age in SGA children based on their deciduous teeth enamel defect severity, and the severe defect refered to slower permanent teeth dental age than the mild defect.

The comparison of deciduous teeth eruption pattern in small gestational age (SGA) and appropriate gestational age (AGA) children Irawati Kirana; Roosje Rosita Oewen; Williyanti Soewondo
Padjadjaran Journal of Dentistry Vol 22, No 2 (2010): July 2010
Publisher : Faculty of Dentistry Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/pjd.vol22no2.26850

Introduction: Small Gestational Age (SGA) children is defined as infant whose birth’s weight was below the tenth percentile intrauterine growth and development curve of Lubchenco. There are two types of SGA children, namely symmetrycal SGA (the disturbance occurred in the first trimester of pregnancy) and asymmetrical SGA (the disturbance occured in second and third trimesters of pregnancy). The aim of this research was to obtain the deciduous teeth eruption patterns in symmetrical and asymmetrical of SGA children, based on baby teeth that have erupted the eruption of primary teeth. Methods: The research design was descriptive and analytical, with cross-sectional design and using the given sample size. The sample were 28 SGA children aged 1-4 years and 33 Appropriate Gestational Age (AGA) children. Determination of SGA children based on Ponderal Index. Eruptive stage was determined by Nola Modification Scoring by the score between 0-4. (Score (0) for tooth that have not erupted; (1) tooth emerge, (2) ⅓ crown erupted tooth, (3) ½ crown erupted tooth, (4) full crown tooth. Results: The results showed, that there were differences in the pattern of deciduous teeth eruption based on teeth eruption stages that have been erupted. Eruption pattern of SGA children deciduous teeth were slower than the eruption pattern of AGA children deciduous teeth. Conclusion: Different patterns of deciduous teeth eruption in symmetrical SGA children and asymmetrical SGA children were showed. Viewed from the stand point of view the stage of teeth eruption, the deciduous teeth eruption pattern of symmetrical SGA children was slower than the asymmetrical SGA children.

The effects of ethyl acetate fraction of Ananas Comosus (L.) Merr. of tongue cancer cell growth inhibition Supri’s Clone-1, invitro Maureen Martina; Roosje Rosita Oewen; Eriska Riyanti; Achmad Syawqie; S. Supriatno
Padjadjaran Journal of Dentistry Vol 23, No 2 (2011): July 2011
Publisher : Faculty of Dentistry Universitas Padjadjaran

Ananas comosus (L.) Merr has several purposes which include antioxidant and anti-inflammatory activity that shows some pharmacological effects and the subject of anti-cancer or anti-cancer supporting material. The research objective was to analyze the effects of ethyl acetate fraction of Ananas comosus (L.) Merr. of tongue cancer cell growth inhibition Supri’s clone-1 (SP-C1). This type of study was a research laboratory. Next, cell growth inhibition testing by the ethyl acetate fraction of Ananas comosus (L.) Merr. with various concentrations (0; 62.5; 125; 250; 250; 500 and 1000 microgram/ml) using the MTT assay test. Growth barriers identified by Biorad microplate tool reader with a wavelength of 540 nm. The number of SP-C1 cells examined was 2 x 104 cells/wells with incubation time 24 and 48 hours. Data were analyzed using a two-ways ANOVA followed by post hoc test (LSD test) with 95% significance level. The results showed ethyl acetate fraction of Ananas comosus (L.) Merr. able to inhibit the growth of cancer cells SP-C1. Various concentrations of ethyl acetate fraction of pineapple were highly significant, meaning that the concentration effect on cell growth of SP-C1. Similarly, incubation time effect on the growth of SP-C1 cells that were very meaningful. The biggest obstacle effect of ethyl acetate fraction of Ananas comosus (L.) Merr. occurred at a concentration of 1000 ug/ml (43.45%) with an incubation time of 48 hours. Conclusion of this study was the fraction of ethyl acetate Ananas comosus (L.) Merr. has the effect of inhibiting the growth of cancer cells SP-C1.

The effects of temporo mandibular joint disorder with clicking symptom on children mastication performance in Deutero Malay race children aged 12-15 years Henri Hartman; Arlette Suzy Puspa Pertiwi; Jakobus Runkat; Yetty Herdiyati; Roosje Rosita Oewen
Padjadjaran Journal of Dentistry Vol 26, No 3 (2014): November 2014
Publisher : Faculty of Dentistry Universitas Padjadjaran

Temporo Mandibular Joint Disorder (TMD) could be caused by forward head posture. Articular sound/TMJ clicking is the most often sign and symptom for TMD that could happen in human being. The presence of TMD such as TMJ clicking would cause an imbalance masticatory system. The purpose of this research is to investigate TMJ clicking effects to masticatory performance. This research was cross-sectional study with a type of epidemiology survey. Subject were children aged 12-15 years old Deutero-Malay sub-races Live in Bandung and was taken using multi-stage random sampling technique. Subject; consisted of 24 children as control group and 28 children as TMJ clicking group. Both group were then checked for masticatory performance using multiple sieve method and 20x chewing of artificial test food. Mastication performance value represented by median particle size (MPS) particle distribution (b) for each group. MPS from TMJ clicking group (3.0571,SD=0.9990) showed higher value than control group (2.28958,SD=0.66838). Statistic analysis with t-test showed that there’s a significant result in both of group (pvalue=0,0024, α = 0,05). Conclussion, temporo mandibular joint clicking subject has lower masticatory performance.

Ethanol extract of mangosteen (Garcinia Mangostana Linn) peel effect in inhibiting the growth of human tongue cancer cells Supri’s Clone 1, invitro Edi Suanto; Roosje Rosita Oewen; Inne Suherna Sasmita; S. Supriatno; Unang Supratman
Padjadjaran Journal of Dentistry Vol 23, No 2 (2011): July 2011
Publisher : Faculty of Dentistry Universitas Padjadjaran

The incidence of tongue cancer in Indonesia reached 1.01% of all cancers and 42% of oral cavity cancer. Tongue cancer therapies including chemotherapy, radiotherapy, surgery, and all three combined therapy. Search for anti-cancer drugs currently switched on herbal plants, one of which is the mangosteen. Has the properties of mangosteen peel extract inhibited the growth of cancer cells. The purpose of the study, obtain IC50 of ethanol extract of mangosteen peel in inhibiting the growth of human tongue cancer cells SP-C1. Research carried out on 96 preparations of human tongue cancer SP-C1 were incubated with ethanol extract of mangosteen peel, preparations were classified in two groups of incubation time (24 hours and 48 hours) and each group will be given preferential treatment over 6 randomly different concentrations: 0 (control), 62.5 μg/mL, 125 μg/mL, 250 μg/mL, 500 μg/mL and 1000 μg/mL. Model experiments were 2 x 6 factorial experiment with eight replication for each cell. Test results with ANAVA, incubation (24 and 48 hour) SP-tongue cancer cells with various concentrations of C1 ethanol extract of mangosteen peel gives a highly significant, indicating differences cancer cell growth inhibition. Incubation time factor showed the long incubation effect on cancer cell growth inhibition. Furthermore, by Newman Keuls test, showed 500μg/mL concentrations of 24-hour incubation had the best effect. Conclusion of the study of ethanol extract of mangosteen peel could achieve with IC50 values of cell growth resistance 50.3% at a concentration of 500 μg/mL and an incubation time of 24 hours.

The relation between enamel defects in deciduous teeth, nutritional status and the development of basic skills in children with small for gestational age Katina Miguna; Williyanti Soewondo; Roosje Rosita Oewen; Eddy Fadiyana
Padjadjaran Journal of Dentistry Vol 24, No 3 (2012): November 2012
Publisher : Faculty of Dentistry Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/pjd.vol24no3.26834

Introduction: Small for Gestational Age (SGA) babies are those whose birth weight are less than 2 SD or below the 10th percentile curve of Lubchenco intrauterine growth and development. The cause of SGA is due to an Intra-uterine growth restriction (IUGR). IUGR inhibits the development of various organs, including the enamel of deciduous teeth and the central nervous system (CNS). The research aims to analyzed correlation between enamel defects in deciduous teeth, nutritional status and the development of basic skills in children with SGA. Methods: A total of 28 SGA children aged between 3-6 years were obtained from Hasan Sadikin Hospital. The sample comprised of 20 boys and 8 girls to be studied for tooth enamel defects in deciduous teeth, weight, height and development basic capabilities. Study design was descriptive and analytical, with cross-sectional study. Scoring modification Developmental Defect of Enamel (DDE) of the FDI was used to assess the enamel defects in deciduous teeth. Enamel Defect Score (EDS) was calculated according to the Willyanti’s formula. The nutritional status was measured by measuring height, weight, and age and converted to the diagram of Body Mass Index (BMI) / Age of the WHO Child Growth Standards. While the development of SGA children basic skills was measured using pre- screening questionnaire development (KPSP). To determine the linkage between enamel defects in deciduous teeth and nutritional status with basic skills development in SGA children, the non-parametric statistical analysis of Kendall’s Coefficient of Concordance was performed. Results: Statistical test results showed that Kendall’s Coefficient of Concordance (W) = 1.00 is statistically significant with Chi-square value = 56.00 with p (value) <0.01. Conclusion: There is a significant relationship between EDS and nutritional status with the SGA children development of basic skills as well as the development of the child’s ability.

The effect of ethanol extract of mangostene (Garcinia Mangostana Linn.) peel on tongue cancer cells Supri's Clone-1 apoptosis, in vitro Maria Aurora Titiana; Roosje Rosita Oewen; Mieke Hemiawati Satari
Padjadjaran Journal of Dentistry Vol 23, No 3 (2011): November 2011
Publisher : Faculty of Dentistry Universitas Padjadjaran

Apoptosis or programmed cell death serves to regulate physiological and pathological conditions. Garcinia mangostana Linn. is one of the medical herbs that is widely used to suppress human tongue cancer growth by inducing apoptosis. The research aimed to analyze the effect of ethanol extract of Garcinia mangostana Linn. on tongue cancer cells apoptosis Supri's Clone-1 (SP-C1). The type of the research was an experimental laboratory. Tongue cancer cells SP-C1 were treated by various ethanol extract concentrations (0, 300, 400, 500, 600, 700 pg/ml) of Garcinia mangostana Linn. to detect the apoptosis, which was done by acridine orange and ethidium bromide coloring tests. The number of tongue cancer cells SP-C1 2x104 cells/dish. Observation on cells apoptosis was observed by a fluorescent microscope with 40 x magnification. The data were analyzed using one-way ANOVA and was followed by Post Hoc test (Tukey-test) with 95% significance level. The result showed that tongue cancer cells SP-C1 apoptosis treated by various ethanol extracts of Garcinia mangostana Linn. increase significantly. The highest effect of apoptosis was detected at the 700 pg/ml concentration that has an effect on the percentage of tongue cancer cells apoptosis by 65%.

Influence of surface modification to stainless steel on initial Streptococcus mutans adhesion and antibacterial acivity Nurhaerani Nurhaerani; Roosje Rosita Oewen; Yetty Herdiyati
Padjadjaran Journal of Dentistry Vol 22, No 2 (2010): July 2010
Publisher : Faculty of Dentistry Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/pjd.vol22no2.26846

Introduction: Stainless steel is one of the most frequently used material in pediatric dental clinic for stainless steel crown, bracket, molar band and archwire. The purposes of this study were to evaluate the surface characteristic (chemichal composition, hydrophobicity, fluoride release), antibacterial activity of modified stainless steel and to evaluate the initial adhesion S. mutans on the surface of modified stainless steel. Methods: This study was an experimental laboratory. Sample used in this study was Stainless steel which modified with the plasma based fluorine ion implantation. The stainless steel which was fluorine ion implanted by CF4 gas was washed in a ultrasonic bath containing distilled water for 10 minute. The modified stainless steel materials were washed in an ultrasonic bath containing distilled water for 10 minute. Stainless steel material was incubated at 37°C in 2 ml of BHI broth containing S. mutans with concentration 2x107 CFU/ml. After 48 hours incubation, a 0.5 ml of solution was immediately transferred into 4.5 ml of PBS (-) and diluted. A 100 pi of diluted solution was plated on BHI agar. After 48 hours culture at 37°C, the number of colonies was counted. The numbers of fluorine ion implanted material and fluorine ion non-implanted control were ten, respectively. Results: XPS analysis showed the presence of fluorine and chromic fluoride until second layer. The contact angle of modified stainless steel was significantly increased A small amount of fluorine ion released from surface of modified stainless steel was detected until the second day. Fluorine ion implanted of stainless steel was significantly decreased the initial adhesion and increased the antibacterial activity. Conclusion: The adhesion of S. mutans on the surface of stainless steel for 4 hours incubation is shown in Figure 11. Fluorine ion implanted stainless steel showed less bacterial adhesion than fluorine ion non- implanted control (p<0.001).

Anti-tumor agent celecoxib activity towards SP-C1 tongue cancer cells invasion (in vitro) Harun Achmad; Mieke Hemiawati Satari; Roosje Rosita Oewen; S. Supriatno
Padjadjaran Journal of Dentistry Vol 23, No 1 (2011): March 2011
Publisher : Faculty of Dentistry Universitas Padjadjaran

Invasion is a characteristic of the occurrence of cancer and indicates the cancer cells' capability to destroy and degrade the border between the epithet and basal membrane to further spread into the surrounding extra-cellular matrix. The purpose of this research was to find the existence of impediment at the SP-C1 tongue cancer cell using celecoxib chemopreventive medication. The SP-C1 tongue cancer cells were treated in vitro using celecoxib medication as a research subject at the following concentrations 5, 10, 25, 50, 75, 100, 125%; and 0 as control group (only DMEM growth medium treatment). Pure experimental testing was carried out for 24 and 48 hours, with observation and calculation of an average number of SP-C1 tongue cancer cells. The data collected were analyzed using the ANOVA test with Newman Keuls paired range test or t-test. Research results indicated that the average number of SP-C1 tongue cancer cells invasion after administration of celecoxib medication based on administration concentration and time statistically yielded significant results. The ANOVA test results were statistically significant, that is, average occurrence of the number of SP-C1 tongue cancer cells due to the use of celecoxib at certain concentrations compared to that without celecoxib was different. At celecoxib of zero (control) concentration was 24.4 with celecoxib concentration starting at 5 up to 125% experienced a decline from its average 11 to become 2.3. The conclusion of the research was that the greater the celecoxib concentration administered, the greater the effect on the impediment of SP-C1 tongue cancer cell invasion.

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