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All Journal Jurnal Kesehatan Masyarakat Biology, Medicine, & Natural Product Chemistry Majalah Obat Tradisional Unnes Journal of Public Health
Budi Mulyaningsih
Faculty of Medicine, Universitas Gadjah Mada
Biochemistry, Genetics & Molecular Biology Health Professions Medicine & Pharmacology Public Health

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INSENCE COMBUSTIBLE SEREH, CENGKEH DAN JERUK SEBAGAI PENOLAK NYAMUK AEDES AEGYPTI Mulyani, Sri; Mulyaningsih, Budi; Lestari, Anindita Winda; Munawaroh, Fitri Ana; Anna, Diescendy Selly
Majalah Obat Tradisional Vol 18, No 3 (2013)
Publisher : Faculty of Pharmacy, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (245.532 KB) | DOI: 10.14499/mot-TradMedJ18iss3pp195-200

Abstract

Nyamuk merupakan salah satu serangga vektor penyakit seperti demam berdarah, malaria, filariasis, dan sebagainya. Penggunaan bahan yang memiliki aktivitas repelan (penolak) seperti sereh, daun jeruk, daun cengkeh merupakan salah satu cara untuk melindungi diri dari gigitan nyamuk. Sediaan dupa (insence combustible) dikenal sebagai sediaan aromaterapi yang dapat digunakan setiap saat, yang umumnya digunakan pada acara-acara keagamaan. Pada penelitian ini dimaksudkan untuk membuat sediaan insence combustible (dupa) yang dapat berfungsi ganda, yaitu sebagai penyegar ruangan juga dapat berfungsi sebagai repelan terhadap nyamuk Aedes aegypti. Penelitian dilakukan dengan membuat sediaan insence combustible dengan  bahan aktif herba sereh, daun cengkeh, dan jeruk, dengan 5 variasi kadar 0,10, 20, 40, 80%. Kemudian sediaan diujikan pada 25 ekor nyamuk, yang terdapat di dalam sangkar nyamuk dobel trap. Jumlah nyamuk di kotak A dan B  dihitung pada waktu 5, 10, 15, 30, 45, dan 60 menit. Selanjutnya dihitung persentase aktivitas repelannya. Juga dilakukan pengujian profil kromatogram bahan uji dengan metoda mikrodestilasi tanur TAS dengan beberapa pereaksi identifikasi. Hasil menunjukkan, bahwa sediaan yang dibuat semua memiliki aktivitas penolak nyamuk dengan kadar efektif  untuk sereh adalah 40%, jeruk 20%, dan cengkeh 10%.  Profil kromatogram menunjukkan daun jeruk memperlihatkan bercak paling banyak untuk senyawa terpenoid dan fenolik, diikuti cengkeh dan sereh. Bercak sebagai senyawa dengan gugus karbonil (aldehid dan keton) hanya ditunjukkan oleh herba sereh.
The Phytochemical Analysis of Hay Infusions and Papaya Leaf Juice as an Attractant Containing Insecticide for Aedes Aegypti Cahyati, Widya Hary; Asmara, Widya; Umniyati, S Rahmah; Mulyaningsih, Budi
Jurnal Kesehatan Masyarakat Vol 12, No 2 (2017): Jurnal KEMAS Vol.12 No.2 : January 2017
Publisher : Jurusan Ilmu Kesehatan Masyarakat Fakultas Ilmu Keolahragaan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/kemas.v12i2.6223

Abstract

Aedes aegypti mosquito population could be controlled by using lethal ovitrap. The addition of hay infusions as a attractant greatly enhance Aedes aegypti eggs trapped, and papaya leaf juice may inhibit Aedes aegypti eggs evolve to larvae or a larvae to pupae stage. This study was conducted to find out the chemical compounds in hay infusion and papaya leaf juice. We used phytochemical test using UV-Vis Spectrophotometry, Thin Layer Chromatography, and High Performance Liquid Chromatoraphy (HPLC) method. The results showed that hay infusion contains 12,75 mg/L ammonium and <1,20 ppm (µg/mL) lactic acid and papaya leaf juice contains 0,25% alkaloid, 0,14% flavonoid, 0,30% saponin, ≤68 mg/L steroid and 11,34% tannin, but negative terpenoid. We concluded that hay infusion and papaya leaf juice contains chemical compounds that could be use as attractant and bioinsecticide to Aedes aegypti, respectively.
Larvicidal Activity of The Mixture of Cashew Nut Shell Liquid (CNSL) and Aqueous Extract of Sapindus rarak DC Against Larvae of Culex quinquefasciatus Fauziah, Rahmi Safarina; Sudarsono, Sudarsono; Mulyaningsih, Budi
Biology, Medicine, & Natural Product Chemistry Vol 3, No 1 (2014)
Publisher : Sunan Kalijaga State Islamic University & Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (244.723 KB) | DOI: 10.14421/biomedich.2014.31.21-23

Abstract

The aim of this study was to evaluate the larvicidal activity of Cashew Nut Shell Liquid (CNSL) against the Culex quinque fasciatus in larval stage. The CNSL was diluted in water by addition of aqueous extract of Sapindus rarak DC to increase its solubility. Larvae were exposed to varying concentrations of that mixture. The larvae mortality was observed after 24 h exposure. LC50 and LC90 value by extrapolation were 20,52 ppm and 55,41 ppm respectively. CNSL were specified by characterizing its physico-chemical properties and  anacardic acid as marker compound by High Performance Chromatography (HPLC). The results were the mixture of Cashew Nut Shell Liquid (CNSL) and Aquous extract of Sapindus rarak DC had larvicidal activity against Cx. Quinque-fasciatus and further investigations were needed to identify the fatty acid derivative as active compound of CNSL which  responsible for larvicidal activity.
The Phytochemical Analysis of Hay Infusions and Papaya Leaf Juice as an Attractant Containing Insecticide for Aedes Aegypti Cahyati, Widya Hary; Asmara, Widya; Umniyati, S Rahmah; Mulyaningsih, Budi
KEMAS: Jurnal Kesehatan Masyarakat Vol 12, No 2 (2017)
Publisher : Department of Public Health, Faculty of Sport Science, Universitas Negeri Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/kemas.v12i2.6223

Abstract

Aedes aegypti mosquito population could be controlled by using lethal ovitrap. The addition of hay infusions as a attractant greatly enhance Aedes aegypti eggs trapped, and papaya leaf juice may inhibit Aedes aegypti eggs evolve to larvae or a larvae to pupae stage. This study was conducted to find out the chemical compounds in hay infusion and papaya leaf juice. We used phytochemical test using UV-Vis Spectrophotometry, Thin Layer Chromatography, and High Performance Liquid Chromatoraphy (HPLC) method. The results showed that hay infusion contains 12,75 mg/L ammonium and <1,20 ppm (µg/mL) lactic acid and papaya leaf juice contains 0,25% alkaloid, 0,14% flavonoid, 0,30% saponin, ≤68 mg/L steroid and 11,34% tannin, but negative terpenoid. We concluded that hay infusion and papaya leaf juice contains chemical compounds that could be use as attractant and bioinsecticide to Aedes aegypti, respectively.
Resistance Status of Aedes albopictus (Skuse) on Malathion in Bengkulu City Triana, Dessy; Umniyati, Sitti Rahmah; Mulyaningsih, Budi
Unnes Journal of Public Health Vol 7 No 2 (2018): Unnes Journal of Public Health
Publisher : Universitas Negeri Semarang in cooperation with Association of Indonesian Public Health Experts (Ikatan Ahli Kesehatan Masyarakat Indonesia (IAKMI))

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (195.976 KB) | DOI: 10.15294/ujph.v7i2.20153

Abstract

Abstract Dengue Hemorrhagic Fever (DHF) is a disease caused by dengue virus and transmitted by Aedes aegypti mosquito as the main vector and Aedes albopictus as secondary vector. In 2016, Bengkulu City was one of 3 cities that experiencing DHF outbreaks in Indonesia. Insecticides malathion have been used since 1990 in bulk in DHF control programs in Bengkulu City and have not had an evaluation report on Aedes sp. vulnerability to malathion. The purpose of this research was to know the resistance status of Ae albopictus mosquitoes from endemic and sporadic areas of DHF in Bengkulu City to malathion. The sample of the study was the Ae. albopictus adult female mosquitoes which collected from endemic and sporadic areas of DHF in Bengkulu City with ovitrap installation. The resistance test was performed by CDC Bottle Bioassay method with malation 96% and 50 μg/ml diagnostic dose of. The results of this study showed 1% mortality on endemic area and 5% on sporadic area. Aedes albopictus of both areas were resistant to malathion. &nbsp; Keywords: Ae. albopictus, bottle bioassay, malathion. &nbsp; Abstrak Demam Berdarah Dengue (DBD) merupakan penyakit yang disebabkan oleh virus dengue dan ditularkan oleh nyamuk Aedes aegypti sebagai vektor utama dan Aedes albopictus sebagai vektor sekunder. Pada tahun 2016, Kota Bengkulu merupakan 1 dari 3 kota yang mengalami KLB DBD di Indonesia. Insektisida malation telah digunakan sejak tahun 1990 secara massal dalam program pengendalian DBD di Kota Bengkulu dan belum memiliki laporan evaluasi mengenai kerentanan Aedes sp terhadap malation. Tujuan penelitian untuk mengetahui status kerentanan nyamuk Ae. albopictus dari daerah endemis dan sporadis DBD Kota Bengkulu terhadap malation. Sampel penelitian adalah nyamuk betina dewasa Ae. albopictus yang dikoleksi dari daerah endemik dan sporadis DBD Kota Bengkulu dengan pemasangan ovitrap. Uji kerentanan dilakukan dengan metode CDC Bottle Bioassay dengan malation 96% dan dosis diagnostik 50 µg/ml. Hasil penelitian ini&nbsp; menunjukkan mortalitas 1% untuk daerah endemis dan 5% untuk daerah sporadis. Aedes albopictus kedua daerah telah resisten terhadap malation. &nbsp; Kata Kunci: Ae. albopictus, bottle bioassay, malation.
Co-Authors Anindita Winda Lestari, Anindita Winda Dessy Triana Diescendy Selly Anna, Diescendy Selly Fitri Ana Munawaroh, Fitri Ana I Dewa Gde Mayun Permana Rahmi Safarina Fauziah, Rahmi Safarina S Rahmah Umniyati, S Rahmah Sitti Rahmah Umniyati Sri Mulyani Widya Asmara Widya Hary Cahyati