Nasril Nasir
Jurusan Biologi Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Andalas

Published : 2 Documents Claim Missing Document
Claim Missing Document
Check
Articles

Found 2 Documents
Search

Pengaruh Faktor Abiotik terhadap Produksi Protease dari Isolat Bakteri M1-23 Mira Rosnawita; Anthoni Agustien; Nasril Nasir
Jurnal Biologi Universitas Andalas Vol 4, No 1 (2015)
Publisher : Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jbioua.4.1.%p.2015

Abstract

A study on the influence of abiotic factors in producing protease by bacterial isolates M1-23 was conducted from May 2013 to December 2013 in the  Laboratory of Microbiology, Department of  Biology, Andalas University, Padang. This study aimed to find optimal conditions by isolate M1-23 to produce protease. This study consists of two stages. First stage used to produce the best protease and the second used to increase enzim homogenity. The result showed that the highest enzyme activity was  at medium temperature 550 C and pH 7.5. The best homogenity was produced at 125 rpm of agitation process.
Teknik Pengujian In Vitro Ketahanan Pisang terhadap Penyakit Layu Fusarium Menggunakan Filtrat Toksin dari Kultur Fusarium oxysporum f. sp. cubense - Jumjunidang; Nasril Nasir; - Riska; H Handayani
Jurnal Hortikultura Vol 15, No 2 (2005): Juni 2005
Publisher : Indonesian Center for Horticulture Research and Development

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jhort.v15n2.2005.p%p

Abstract

Sejauh ini informasi mengenai uji ketahanan pisang terhadap patogen layu fusarium menggunakan filtrat toksin patogennya masih terbatas. Penelitian ini dilaksanakan pada bulan Juni 2002 sampai dengan Maret 2003 di laboratorium kultur jaringan, laboratorium penyakit, dan Rumahkasa Balai Penelitian Tanaman Buah, Solok. Tujuan penelitian ini adalah untuk mendapatkan teknik pengujian dini tingkat in vitro dengan media yang mengandung filtrat toksin dari kultur Fusarium oxysporum f. sp. cubense (Foc) serta mendapatkan tanaman pisang tahan Foc. Rancangan yang digunakan adalah acak lengkap dengan lima perlakuan dan lima ulangan. Setiap ulangan terdiri dari 10 botol, masing-masing berisi satu plantlet. Untuk perlakuan toksin asam fusarat murni, setiap ulangan terdiri dari 2 botol. Perlakuan tersebut adalah konsentrasi 0, 10, 20, 40, dan 60% filtrat toksin asam fusarat dari kultur cendawan Foc dan konsentrasi 0; 0,05; 0,1; 0,2; dan 0,4 μM asam fusarat murni yang berperan sebagai pembanding. Pembuatan perlakuan dilakukan dengan menambahkan filtrat toksin dari kultur Foc dan toksin murni sesuai konsentrasi di atas ke dalam media tumbuh. Filtrat toksin asam fusarat dari kultur Foc pada konsentrasi 60 dan 40% dapat digunakan sebagai media dalam pengujian ketahanan pisang tingkat in vitro. Semakin tinggi konsentrasi filtrat toksin dari kultur Foc dan asam fusarat murni yang ditambahkan, semakin cepat masa inkubasi atau munculnya gejala penyakit. Terjadi fenomena recovery dari tanaman sakit akibat perlakuan toksin murni asam fusarat dan filtrat toksin dari kultur Foc. Filtrat toksin dari kultur Foc berpeluang digunakan sebagai media pengujian dini ketahanan pisang terhadap layu fusarium. In vitro screening techniques for resistance of Musa to fusarium wilt disease by using filtrate toxin from Fusarium oxysporum f. sp. cubense culture. So far, study on the in vitro screening techniques for banana resistant to fusarium wilt disease by using filtrate toxin of its pathogen, was limited. The experiment was conducted at tissue culture laboratory, plant pathology laboratory and screenhouse of Indonesian Fruit Research Institute, Solok from June 2002 to March 2003. The objective of this study was to find out in vitro techniques for selecting of Musa to wilt disease by using filtrate toxin from Fusarium oxysporum f. sp. cubense (Foc) culture and to find out resistant plant. The experiment was arranged in a randomized completely design with five treatments (concentration of filtrate toxin 0; 10; 20; 40; and 60%) and five replications, each replications consist of ten bottle of plantlets. The consentration of fusaric acid used as comparison were 0; 0.05; 0.1; 0.2; and 0.4 μM. The results showed that concentration of filtrate toxin fusarium culture at 60 and 40% could be used as a selection medium for in vitro screening techniques for Musa resistance to Foc. The higher concentration of filtrate toxin of Foc culture and fusaric acid, the faster disease incubation periode appeared. There was recovery of attacked plant due to fusaric acid and filtrate toxin of culture Foc treatment. This filtrate can be used as an early testing medium for resistance to fusarium wilt of banana.