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All Journal Jurnal Sains dan Teknologi Health Notions Meditory : The Journal of Medical Laboratory Jurnal Pengabmas Masyarakat Sehat International Journal of Islamic Education, Research and Multiculturalism (IJIERM) Jurnal Pengabmas Masyarakat Sehat

Nyoman Mastra

Poltekkes Kemenkes Denpasar

Author-ID : 336305

Religion Aerospace Engineering Arts Humanities Biochemistry, Genetics & Molecular Biology Computer Science & IT Decision Sciences, Operations Research & Management Dentistry Education Environmental Science Health Professions Medicine & Pharmacology Nursing Public Health Veterinary Other

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UJI AKTIVITAS ANTIBAKTERI EKSTRAK ETANOL DAUN JARAK PAGAR (Jatropha curcas Linn) PADA BERBAGAI KONSENTRASI TERHADAP PERTUMBUHAN BAKTERI Pseudomonas aeruginosa nyoman mastra
Meditory : The Journal of Medical Laboratory Vol 9, No 2 (2021): Volume 9, nomer 2, Desember 2021
Publisher : Jurusan Analisis Kesehatan, Poltekkes Kemenkes Denpasar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33992/m.v9i2.1736

Background, Pseudomonas aeruginosa is the main bacterium in nosocomial infections. Pseudomonas aeruginosa is a bacterium that is resistant to almost all of antibiotics. As a result of these resistance cases, it is necessary to find the alternative products derived from natural ingredients antibacterial agents, one of which is Jatropha leaves. Objective, the objective of this study was to know the antibacterial activity of ethanol extract of Jatropha leaves at concentrations of 20, 40, 60 and 80% against the growth of Pseudomonas aeruginosa. Method, This study used experimental posttest only control group design with disc diffusion method used concentrations of 20, 40, 60 and 80%. Positive control using ciprofloxacin antibiotics and negative control using 96% ethanol. Results, of the study obtained the average diameter of inhibitory zones at concentrations of 20, 40, 60%, and 80% respectively were 11.5 mm, 18.3 mm, 19.5 mm, and 20.7 mm. Respectively the analysis of Least Significant Difference test, it was found that there were significant differences in inhibition zones in each extract concentration. Conclusion, the conclusion of this study is that the ethanol extract of Jatropha leaves has antibacterial activity against the bacterium Pseudomonas aeruginosa. Keywords: Jatropha leaves, extract, antibacterial activity, Pseudomonas aeruginosa

TINJAUAN TENTANG KOLESTEROL DARAH PADA SOPIR BUS PARIWISATA DI KABUPATEN BADUNG nyoman mastra
Meditory : The Journal of Medical Laboratory Vol 5, No 1 (2017): Juni 2017 Meditory
Publisher : Jurusan Analisis Kesehatan, Poltekkes Kemenkes Denpasar

ABSTRACT Background The lifestyle of consuming high fatty foods, smoking and drinking coffee frequently in the work place could be expected to cause high cholesterol that affects the health of the tourism bus driver. Objective The aims study to determine a cholesterol levels in tourism bus driver at Badung regency. Methods This research is a descriptive study. Tourism bus driver who works at Indah Putra Transport. Result Of the 39 respondents obtained 18% had normal cholesterol levels and 82% had high cholesterol levels. Based on the frequency of smoking every day, the highest frequency indicated by smoking more than 20 cigarettes per day by 25 respondents (64%). The frequency of coffee consumption < 5 cups per day, 41% had high cholesterol levels, consume coffee > 5 cups of coffee per day, 41% have cholesterol levels high. Based on Physical activity habits 3˗4 times a week indicated 5% had high cholesterol levels and physical activity < 3 times a week indicated 77% had high cholesterol levels. 1˗5 year working period earned as much as 8% had high cholesterol levels, 6˗10 year working period earned 3% had normal cholesterol levels and 23% had high cholesterol and the rest of the respondents were 51% in working period above of 11 years have high cholesterol levels. Conclution It can be conceded that bad lifestyle of tourism bus drivers can affect the level of cholesterol. Keywords : driver, cholesterol, lifestyle

KADAR SERUM GLUTAMATE PIRUVAT TRANSAMINASE PECANDU MINUMAN KERAS DI BANJAR AMBENGAN DESA SAYAN UBUD GIANYAR nyoman mastra; I Gusti Ayu Tari Diva Pradnya Dewi; I Wayan Merta
Meditory : The Journal of Medical Laboratory Vol 4, No 2 (2016): Desember 2016
Publisher : Jurusan Analisis Kesehatan, Poltekkes Kemenkes Denpasar

Background Banjar Ambengan is one banjar contained in Sayan village, where people still have the habit of gathering accompanied by excessive alcohol consumption. Consumption of alcohol has a direct relationship with the death cases caused by liver sirosis. The liver mechanism disturbance leads to the case of swelling as the result of the rise of serum glutamate piruvat transminase (SPGT) enzyme. Objective This study is aimed at investigating the SPGT level of the serum of the alcoholic. The study uses the non-probability sampling technique called snowball sampling. The samples of this study are thirty alcoholic men aging upper eighteen. The respondents will be interviewed to get the deeper information. The research reveals that 6 respondents have high SPGT level (20%) and 24 of them have normal SPGT level (80%). Result of the examination serum SGPT levels alcoholics in the study ranged from 11 to 79.20 U / L. The measurement results SGPT levels are high on the research respondents based on the characteristics of age at most 33.3%, based on the frequency of consumption of at most 33.3%, based on the period of consumption at most 50%, based on the volume of consumption at most 66.7%, based on the type of liquor consumed at most 66.7%, and based on the type of work at most 50%. Conclusion Keywords: Liquor, Liver, Addict, SGPT Levels

PERBEDAAN ZONA HAMBAT PERTUMBUHAN Staphylococcus aureus PADA BERBAGAI KONSENTRASI EKSTRAK ETANOL DAUN BIDURI SECARA IN VITRO nyoman mastra
Meditory : The Journal of Medical Laboratory Vol 6, No 1 (2018): Meditory, volume 6 , nomer 1 , Juni 2018
Publisher : Jurusan Analisis Kesehatan, Poltekkes Kemenkes Denpasar

ABSTRACTBackground Staphylococcus aureus is a pathogenic bacteria that can cause infection. Biduri leaf has antibacterial compound such as tannin, flavonoid, saponin, and polyphenol.Objective This study aimed to know the difference of growth inhibition zone of Staphylococcus aureus at the various concentration of ethanol extract of biduri leaf and to determine an effective concentration.Methods This study was the true experiment with posttest only control design, used Kirby-Bauer disk diffusion method with five concentrations (20%, 40%, 60%, 80%,100%), positive control (chloramphenicol 30 µg) and negative control (ethanol 96%).Result The result of this study showed the average of inhibition zone diameter of each concentration consecutively 26,2 mm, 28,3 mm, 29,7 mm, 31 mm and 31,5 mm. One Way Anova statistic analysis showed that the value of p is 0.000 so there is the difference of growth inhibition zone of Staphylococcus aureus at various concentrations of ethanol extract of a biduri leaf.Conclusion The conclusion of this study is there are differences in growth inhibition zone of Staphylococcus aureus at various concentrations of ethanol extract of biduri leaf and an effective concentration that can inhibit of Staphylococcus aureus is concentration 20% with mean inhibition zone is 26,2 mm. Keywords: ethanol extract of biduri leaf; Staphylococcus aureus; inhibition zone

PERBEDAAN ZONA HAMBAT PERTUMBUHAN Staphylococcus aureus PADA BERBAGAI KONSENTRASI REBUSAN DAUN SALAM (Syzygium polyanthum) SECARA IN VITRO nyoman mastra
Meditory : The Journal of Medical Laboratory Vol 5, No 2 (2017): meditory ,Volume 5, No 2, Desember 2017
Publisher : Jurusan Analisis Kesehatan, Poltekkes Kemenkes Denpasar

AbstractBackground Staphylococcus aureus is one of causes infection and this bacteria have been resistence for many antibiotic. Bay leaf have antibacterials substance, which stew leaves can be treat infection caused Staphylococcus aureus. The purpose of this study was to determine differences in growth inhibition zone of Staphylococcus aureus at various concentrations of water stew of bay leaf.Method The method of this study is true experiment with posstest only control design, and used Kirby Bauer disk diffusion method with various concentrations of water stewed of bay leaf (20%, 40%, 60%, 80%,100%), positive control (chloramfenicol 30 μg) and negative control (sterile distilled water). Result The result showed that the average diameter of inhibition zone in concentration 20%, 40%, 60%, 80%, and 100% is 7 mm, 8,4 mm, 9,6 mm, 10,5 mm and 11,5 mm. Based on statistical analysis using oneway ANOVA available the value of p (0,000) α (0,05), so the inhibition zone is significant difference of growth inhibition zone of Staphylococcus aureus at various concentrations of stewed water bay leaf. Conclusion Water stew of bay leaf can inhibit the growth of Staphylococcus aureus, and there are differences in growth inhibition zone of Staphylococcus aureus at various concentrations of water stew of bay leaf. Keywords: stew of bay leaf; Staphylococcus aureus; inhibition zone

UJI AKTIVITAS ANTIBAKTERI REBUSAN DAUN SIRIH (Piper betle Linn) TERHADAP BAKTERI Streptococcus pyogenes nyoman mastra
Meditory : The Journal of Medical Laboratory Vol 7, No 1 (2019): Meditory, volume 7 , nomer 1 , Juni 2019
Publisher : Jurusan Analisis Kesehatan, Poltekkes Kemenkes Denpasar

ABSTRACTBackground: Infection is a disease caused by pathogenic microbes and is very dynamic. ISPA is defined as an acute respiratory disease caused by infectious agents that were transmitted from human to human. One of the microbes that caused ISPA is Streptococcus pyogenes, which is a gram-positive bacteria and belongs to Streptococcus sp. β-hemolytic class A. Nowadays, many people are begin to use natural materials to deal with microbial infections. One of them is the betel leaf (Piper betle Linn.) Which has antibacterial and antibiotic components for the treatment of respiratory problems, and bacterial infectious diseases.Purpose: This research was conducted with the aim to know the antibacterial activity of betel leaves (Piper betle Linn) decoction against Streptococcus pyogenes bacteria. Methods: This research used true experimental design with Posttest Only Control Design, and data analysis with One Way Anova and Least Significant Deference (LSD). Betel leaves decoction water is made in concentrations of 20%, 40%, 60%, 80%, and 100%, then tested on Streptococcus pyogenes bacteria with two replicates and four repetitions.Result: From a total of 40 data obtained, the mean diameter of the inhibitory zone at each concentrations is 20% 0 mm, 40% 12.9 mm, 60% 22.7 mm, 80% 25.5 mm, and 100% 30 mm.Conclution: Betel leaves decoction water is able to inhibit the growth of Streptococcus pyogenes with significant difference of drag zone diameter at each concentration. Keywords: Antibacterial, Betel Leaves, Streptococcus pyogenes

IDENTIFIKASI JAMUR Candida albicans PADA URINE WANITA MENOPAUSE DI BANJAR TAMAN DESA DARMASABA KECAMATAN ABIANSEMAL KABUPATEN BADUNG nyoman mastra
Meditory : The Journal of Medical Laboratory Vol 8, No 1 (2020): Meditory, vol. 8 no 1 Juni 2020
Publisher : Jurusan Analisis Kesehatan, Poltekkes Kemenkes Denpasar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33992/m.v8i1.1084

ABSTRACTBackground: menopausal women are a predisposing factor for candidiasis. In menopausal women humoral changes occur in the body and pH in the vagina. This change in pH can lead to increased fungal growth of Candida albicans. Purpose: this study aims to identify the fungus Candida albicans in urine samples of menopausal women at Banjar Taman, Darmasaba Village, Abiansemal District, Badung Regency. Method: this research was conducted descriptively with a cross sectional approach. The sampling technique with Non-probality sampling is Purposive Sampling. Urine samples were cultured on SDA media. Result: the growing colonies were identified macroscopically and microscopically and tested germ tube. Identification results showed that from 33 urine samples, 5 (15%) were positive, there was a Candida albicans fungus which showed protruding from the surface of the media, the surface on the colonies was smooth, yellowish white, and had a yeast odor. On microscopic examination it was found that yeast, pseudohifa, clamidospora, blastospora and germ tube. Conclusion: the percentage value shows the occurrence of Candida albicans fungi in the urine of menopausal women in Banjar Taman, Darmasaba Village, Abiansemal Subdistrict, Badung Regency so that menopausal women are expected to always maintain personal hygiene and routinely conduct microbiological examinations.Keywords: urine, menopausal women, candida albicans

GAMBARAN ANGKA LEMPENG TOTAL DAN IDENTIFIKASI BAKTERI Escherichia coli PADA LOLOH BUNGA TELENG DI DESA PENGLIPURAN KECAMATAN BANGLI nyoman mastra
Meditory : The Journal of Medical Laboratory Vol 9, No 1 (2021): Meditory, Volume 9, No 1, Juni Tahun 2021
Publisher : Jurusan Analisis Kesehatan, Poltekkes Kemenkes Denpasar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33992/m.v9i1.1307

AbstractBackground : Teleng flower herb is a processed beverage made from teleng flower which is popular as a traditional drink from Penglipuran Village. As one of the beverage products, teleng flower herbs have to obtain consent from the BPOM before distributed and consumed. Purpose : this study was conducted to analyze the quality of microbiology of teleng flower herb. The analyzed parameters were total plate count (ALT) and E. coli bacteria identification. Method : this study used descriptive research with laboratory examinations which was conducted at the Bacteriology Laboratory. The 7 samples were obtained from producers with three times repetition so that it became 21 samples with sampling techniques were saturated sampling. Result : The ALT examination results show that 5 samples (71.4%) are not qualify and 2 samples (28.6%) are qualify based on BPOM Regulation Number 16 In 2016. The obtained results of E. coli identification show that there were 4 (57.1%) contain of E. coli pathogens and 3 samples (42.9%) are not contains of E. coli pathogens. It can be concluded that most of the hygiene of teleng flower herb at Penglipuran Village does not meet the requirements. Conclusion : Based on these results, we suggest to the producers of teleng flower herb to maintain the quality of teleng flower herb that they sell. Key words: teleng flower herb, microbiological quality, total plate count, E. coli

UJI AKTIVITAS ANTIBAKTERI EKSTRAK ETANOL AKAR ENCOK (Plumbago zeylanica L.) TERHADAP PERTUMBUHAN Pseudomonas aeruginosa SECARA IN VITRO nyoman mastra
Meditory : The Journal of Medical Laboratory Vol 7, No 2 (2019): Meditory , vol7, no 2, 2019
Publisher : Jurusan Analisis Kesehatan, Poltekkes Kemenkes Denpasar

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (510.042 KB)

ABSTRACTBackground Pseudomonas aeruginosa is negative gram bacteria which cause urinary tract infection. Encok root contains tannins, alkaloids, saponins, tri-terpenoids and flavonoids. The purpose This purpose is to observe the antibacterial activities on encok root ethanol extract with various concentrations towards Pseudomonas aeruginosa bacteria growth. The Method The type of experiment used true experiment with posttest only control group design using Kirby-Bauer diffusion discs method on five concentrations (20%, 35%, 50%, 65% and 80%), work controls (Ciprofloxacin 5µg) and negatif control (96% ethanol). The result showed that the average diameter of inhibition zone of encok root ethanol extract at concentration 20% (10,5 mm), 35% ( 11,7 mm), 50% (11,8 mm) 65% (12,3 mm) and 80% (15,3 mm). These five concentration have medium until strong inhibition potential. Statistic analysis with One Way Anova the value of p(0,000)α(0,05). In Least Significant Difference test showed the significant different of inhibition zone in each extract concentration. Conclusion This examination showed that antibacterial activity of ethanol extract of encok root with various concentrations (20%, 35%, 50%, 65% and 80%) was difference in towards the growth of Pseudomonas aeruginosa bacteria.Keyword: Encok roots, antibacterial activity, Pseudomonas aeruginosa

GAMBARAN KADAR ENZIM KOLINESTERASE DALAM DARAH PADA KELOMPOK TANI MEKAR NADI DI DESA BATUNYA KECAMATAN BATURITI nyoman mastra
Meditory : The Journal of Medical Laboratory Vol 6, No 2 (2018): Meditory, volume 6, nomer 2, Desember 2018
Publisher : Jurusan Analisis Kesehatan, Poltekkes Kemenkes Denpasar

Background: Pesticides is poison and dangerous materials. It can cause negative effects to human health directly or indirectly. Blood cholinesterase enzyme analysis is one of the methods to indicate that someone is poisons by pesticide. Purpose: This research is a descriptive study. The aim of this study is to illustrate the cholinesterase levels in the farmer’s blood especially the farmers in Batunya village. Methods: The population in this research was 30 farmers who are the members of Groups Tani Mekar Nadi. The sampling method that used in this research was saturation sampling. Data collection used the questionnaire. The test-mateChE cholinesterase test system (model 400) is the device that used to check the level of cholinesterase in the farmers’ blood. Result: The result show that 22 peoples (73,3%) cholinesterase level was abnormal. In the other hand, 8 of the farmer (26,7%) has normal level of cholinesterase. Conclusion: From the result it can be concluded that it’s still important to monitor the level of cholinesterase in farmers’ blood to increase awareness towards the danger of pesticide, always use self-protection device, and conducting a good pesticide management.Keywords: pesticide, cholinesterase, poisoned

Co-Authors Cok Dewi Widya hana Sundari Heri Setiyo Bekti I Gede Sudarmanto I Gede Sudarmanto I Gusti Agung Ayu Dharmawati I Gusti Agung Dewi Sarihati I Gusti Ayu Sri Dhyanaputri I Gusti Ayu Tari Diva Pradnya Dewi I Nyoman Jirna I Nyoman Jirna I Nyoman Purna I Wayan Juniarsana I Wayan Merta I Wayan Merta I Wayan Sudiadnyana Ida Ayu Made Sri Arjani Kadek Eliana Kesuma Dewi Ni Ketut Rusminingsih Ni Komang Juniawati Ni Nyoman Dewi Supariani Nur Habibah

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