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Nur Khasanah
Department Of Plant Protection, Faculty Of Agriculture, University Of Tadulako Jln. Sukarno Hatta Km. 9 Tondo, Palu, Central Sulawesi 49118 Indonesia
Agriculture, Biological Sciences & Forestry Humanities Control & Systems Engineering Economics, Econometrics & Finance Education Environmental Science Nursing Public Health Social Sciences

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Journal : Jurnal Perlindungan Tanaman Indonesia

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Preliminary Valuation Activity of Calotropis gigantea L. Extracts against Several Insect Pests Nur Khasanah; Edhi Martono; Y. Andi Trisyono; Arman Wijonarko
Jurnal Perlindungan Tanaman Indonesia Vol 26, No 2 (2022): In Progress
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jpti.71938

Abstract

Calotropis gigantea produces secondary metabolites. Some toxicity tests on several pest species had been conducted, yet the most susceptible pest species towards C. gigantea extract is still unknown. This study aims to determine the susceptible insect species and study the behavior of susceptible insects to C. gigantea extract. The test methods used in this research were leaf dipping and spraying upon four insect pest species of different orders (Bactrocera carambolae [Diptera: Tephritidae], Nilapavata lugens [Hemiptera: Delphacidae], Sitophilus zeamais [Coleoptera: Curculionidae], and Plutella xylostella [Lepidoptera: Plutellidae]). The concentration used to determine susceptible insects was 25 g.L-1 and control. Deterrence test of susceptible insects using a choice test and no-choice for 1.5 hours observed every five minutes. Five replication were used for each concentration. Behavioral test of susceptible insects using dietary toxicity test. Five bok choy leaf disc was immersed into solutions for 1 minute and air-dried on a petri dish. Observations were carried out for 12 hours with an observation period of every 1.5 hours. A leaf dipping test for 12 hours with an observation period of every 1.5 hours using the feed method. The concentrations used were 12.5 µgL-1and control. The concentration used was the toxicity test of C. gigantea leaf extract 25, 12.50, 6.25. 3.12, 1.56, 0.78, 0.39, 0.19, 0.09, 0.04 µgL-1. P. xylostella was considered a more susceptible test insect towards C. gigantea leaf extract than B. carambolae, N. lugens, and S. zeamais. C. gigantea leaf extract showed a toxic and feeding deterrent to P. xylostella larvae. The LC50 value of C. gigantea extract against P. xylostella by dipping was 16.9 µg.l-1 and 18.5 µg.l-1 by spray. The components of C. gigantea leaf extract consist of alkaloid, tannin, phenol, flavonoid, saponin, and terpenoid. The result of the research showed that C. gigantea leaf extract has a toxic and deterrent substance against P. xylostella and potential to control P. xylostella.
Co-Authors Abdul Wahid Andi Ete Annam, Agung Chairul Arman Wijonarko Asgar Taiyeb Burhanuddin Haji Nasir Burhanuddin Haji Nasir Burhanuddin Nasir Dance Tangkesalu Dwi Rohma Wulandari Edhi Martono Flora Pasaru Idham Idham Idul Fuqra Johanis Panggeso Moh. Hibban Toana Moh. Yunus Mohammad Yunus Mohammad Yunus Nasir, Burhanudin Nova Alvianita Panggalo Nur Edy Nur Hayati Pagiu, Salapu Pasaru, Remalia Bulo razak, Nurhidayah abd. Riskayanti Riski Kaleb Rosmini Rosmini Shahabuddin Saleh Siti Rahmawila Sri Anjar Lasmini Sulaeman Sulaeman Usman Made Valentino Valentino Y. Andi Trisyono Zulfitri