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All Journal Journal of Health (JoH) Meditory : The Journal of Medical Laboratory Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Jurnal Analis Medika Biosains (JAMBS) BALABA (JURNAL LITBANG PENGENDALIAN PENYAKIT BERSUMBER BINATANG BANJARNEGARA) Journal of Indonesian Medical Laboratory and Science (JoIMedLabS)

Hieronymus Rayi Prasetya

D-III Teknologi Laboratorium Medis, Sekolah Tinggi Ilmu Kesehatan Guna Bangsa, Yogyakarta, Indonesia

Author-ID : 3435728

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Environmental Science Health Professions Immunology & microbiology Medicine & Pharmacology Nursing Public Health Veterinary

Published : 9 Documents Claim Missing Document

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Articles

Perbedaan Waktu Penambahan Reagen AHG Berpengaruh Terhadap Hasil Pemeriksaan Uji Silang Serasi Metode Tabung Syaqina Rassajati; Diani Mentari; Relita Pebrina; Hieronymus Rayi Prasetya
Jurnal Analis Medika Biosains (JAMBS) Vol 9, No 1 (2022): JURNAL ANALIS MEDIKA BIOSAINS (JAMBS)
Publisher : Poltekkes Kemenkes Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32807/jambs.v9i1.267

Transfusi darah adalah proses pemberian darah dari seseorang (donor) kepada orang lain (resipien) yang bertujuan untuk mengganti darah hilang akibat perdarahan, mengatasi shock, memelihara serta mempertahankan kesehatan resipien. Analisis pretransfusi khususnya pada uji serologi sangat diperlukan untuk menjamin darah yang ditransfusikan aman dan tidak menyebabkan reaksi transfusi. Proses pencocokan darah donor dan dengan darah pasien/resipien dilakukan melalui uji. uji silang serasi (crossmatch). Uji silang serasi dapat dilakukan melalui 2 metode yaitu yaitu menggunakan tabung dan gel card. Uji silang serasi metode tabung terdiri dari 3 fase yaitu fase 1, fase 2 dan fase 3. Pada Fase 3 merupakan tahapan yang paling berpengaruh, karena ada penambahan Anti Human Globulin (AHG). Reagen AHG berisi immunoglobulin yang berfungsi sebagai penghubung antibodi inkomplit (antibodi IgG yang menyelubungi eritrosit), sehingga akan terdeteksi melalui terbentuknya aglutinasi (gumpalan). Namun perlu diketahui bahwa ikatan antar antibodi tidak dapat bertahan lama. Ikatan antar antibodi (AHG dan antibodi inkomplit) dapat terlepas kembali (disosiasi), sehingga hasil pemeriksaan dapat menjadi false negatif. Tujuan penelitian ini adalah untuk mengetahui apakah waktu penambahan AHG mempengaruhi derajad aglutinasi pada saat uji silang serasi. Penelitian ini menggunakan metode uji silang serasi metode tabung dengan menggunakan golongan darah O rhesus positif sebagai sampel. Analisis data dilakukan melalui pengamatan derajat aglutinasi pada tiap fase uji silang serasi. Pada penelitian ini, adanya perbedaan waktu penambahan AHG mempengaruhi hasil pemeriksaan uji silang serasi. Kesimpulan dalam penelitian ini adalah efektifitas penambahan AHG pada uji silang serasi metode tabung yaitu pada menit ke 2-10 menit.

Gambaran Anemia pada Lanjut Usia di Panti Sosial Tresna Werdha Abiyoso Yogyakarta Tahun 2013 Hieronymus Rayi Prasetya; Sistiyono Sistiyono; Maria Elisabeth Enjel Naur
Journal of Health (JoH) Vol 1 No 1 (2014): Journal of Health - January 2014
Publisher : LPPM STIKES Guna Bangsa

Background: Anemia is a main health problem of elderly. The aging process causes many psychological changes in nearly all body systems of elderly that causes many kinds of processes of nutrient absorptions in the body disturbed. There is an opinion says that generally elderly has lower hemoglobin levels compared to the younger people. Other research showed that elderly often suffered from calorie and protein malnutrition and micronutrient. It happened because the HCL and pepsin secretion was reduced. Consequently, the absorption of Vitamin B12 and iron decreased that causes Anemia. Method: It was a descriptive analysis with laboratory tests. The sample of this research was 60 year-old and older patients in TresnaWerdhaAbiyoso Nursing Home Yogyakarta. There were 96 patients. The data of research results were analyzed descriptively and presented in the forms of tables and graphs. Results: Of the 96 old patients, there were 31 patients suffered from anemia that consisted of 27 patients with mild anemia and 10 patients with moderate anemia. Based on the morphology of erythrocytes in elderly, there were two kinds of anemia. They were normocytic-normochromic anemia with 84 patients and microcytic-hypochromic anemia with 12 patients. Conclusion: The description of anemia in elderly who stayed in TresnaWerdhaAbiyoso Nursing Home Yogyakarta showed that 32.29% of the elderly suffered from anemia. 72.97% suffered from mild anemia. 27.03% suffered from moderate anemia. The anemia classifications based on morphology in elderly in Tresna Werdha Abiyoso Nursing Home Yogyakarta were 87.5% normocytic-normochromic anemia and 12.5% microcytic-hypochromic anemia.

Perbedaan Hitung Jumlah Trombosit Menggunakan Darah Vena dan Darah Kapiler Hieronymus Rayi Prasetya; Maria Irena Dentri; Sistiyono Sistiyono
Journal of Health (JoH) Vol 3 No 2 (2016): Journal of Health - July 2016
Publisher : LPPM STIKES Guna Bangsa

Background: Platelets play a role in hemostasis which is the body's mechanisms to prevent and stop the bleeding. Platelets participate in the effort to close the wound, so that the body does not experience a loss of blood and protected from foreign cells. Examination of the platelet count is very important in the diagnosis of diseases, one of which is the diagnosis of dengue hemorrhagic fever (DHF). Examination of blood counts, especially platelets in clinical laboratories causes blood samples in use are not always the venous blood but could use capillary blood. Capillary blood samples are used primarily in pediatric patients, because the venous blood sampling is difficult, patient loads, and also shorten the time when taking blood. The purpose of this study was to determine whether there is a difference in counting the number of platelets using samples of blood veins and capillaries. Methods: Quantitative research with observational approach using a cross sectional study design in the 30 samples of student D3 Health Analyst STIKes To Nation Yogyakarta. Statistical methods in use are independent T test. Results: The research subjects were 30 samples of student D3 Health Analyst STIKes To Nation Yogyakarta. The results of the examination of venous blood platelet count and blood capillaries have different average values are 247 530 cells / ml of blood, for blood platelets veins and 184 270 cells / ml of blood for capillary blood platelets. Spearman correlation analysis Obtained results of the examination of venous blood platelet count and blood capillaries normal distribution (p> 0.05). 0.129 venous blood platelet counts, while the number of blood platelets kapilernya 0.089. Conclusion: There is a significant difference from the results of counting the number of blood platelets using veins and capillaries, where the use of capillary blood samples showed that lower platelet counts.

Potensi Etnomedicine Daun Ubi Jalar Ungu (Ipomoea batatas L. Poir) dan Daun Ubi Jalar Putih (Ipomoea batatas L.) Sebagai Obat Demam Berdarah di Sleman DIY Yuliana Prasetyaningsih; Novita Sari; Hieronymus Rayi Prasetya; Visensa Gerosa Naer
Journal of Health (JoH) Vol 6 No 1 (2019): Journal of Health - Januari 2019
Publisher : LPPM STIKES Guna Bangsa

Pendahuluan: Kearifan lokal, pengobatan tradisonal, dan pengetahuan etnobotani perlu dipelajari dan dikembangkan. Salah satu masalah yang masih sering terjadi pada masyarakat adalah munculnya penyakit demam berdarah yang disebabkan nyamuk Aedes Aegypti. Demam Berdarah Dengue merupakan penyakit yang ditakuti karena menurunkan konsentrasi trombosit ke tingkat rendah (trombositopenia) dan dapat menyebabkan perdarahan. Jumlah sel trombosit yang rendah, harus segera ditingkatkan untuk menghindari terjadinya syok. Secara empiris, masyarakat menggunakan air rebusan daun ubi jalar pada kasus-kasus DBD (Demam Berdarah Dengue) dan menunjukkan perbaikan. Penelitian ini bertujuan untuk mengetahui potensi etnomedicine daun ubi jalar ungu (ipomoea batatas l. poir) sebagai obat demam berdarah di wilayah Sleman, DIY. Selain itu juga untuk mengetahui persentase peningkatan jumlah trombosit pada Tikus Putih (Rattus norvegicus) dengan pemberian infusa Daun Ubi Jalar Ungu dan ubi jalar putih (Ipomoea batatas .L) Jenis penelitian: True experimental dengan rancangan penelitian pretest dan post test with control group design. Penelitian ini menggunakan 35 tikus putih jantan yang dibagi dalam 7 kelompok perlakuan infusa daun ubi jalar ungu yaitu kelompok kontrol, kelompok dosis ringan, kelompok dosis sedang dan kelompok dosis berat untuk masing-masing infusa daun ubi jalar. Tikus diturunkan jumlah trombositnya melalui mekanisme kerusakan limpa dengan induksi anilin secara intravena. Setelah 24 jam penginduksian, hewan coba diberi perlakuan sesuai kelompok. Semua kelompok dihitung jumlah trombositnya sebelum dan sesudah perlakuan. Data yang diperoleh dari hasil penelitian dianalisis secara deskriptif yang disajikan dalam bentuk tabel dan narasi. Hasil penelitian: Terjadi peningkatan jumlah trombosit pada kelompok kontrol sebanyak 43%. Pada kelompok infusa daun ubi jalar ungu dosis ringan tidak ada peningkatan, kelompok dosis sedang meningkat sebesar 94%, dan kelompok dosis tinggi diperoleh peningkatan sebesar 224%. Pada kelompok infusa dun ubi jalar putih terdapat peningkatan pada dosis tinggi yaitu 7,2 ml/200gr BB atau 26,46 %. Hasil uji statistik menunjukkan bahwa pemberian infusa daun ubi jalar putih pada pre test dan post test memiliki perbedaan yang bermakna dengan nilai p value 0,023. Sedangkan pemberian infusa daun ubi jalar ungu sebelum dan sesudah perlakuan tidak memiliki perbedaan secara bermakna yang ditunjukkan dengan nilai p value 0,550. Kesimpulan: Infusa daun ubi jalar ungu mampu meningkatkan jumlah trombosit yang lebih banyak dibandingkan dengan infusa daun ubi jalar putih. Infusa daun ubi jalar ungu dan ubi jalar putih memiliki potensi etnomedicine sebagai obat demam berdarah di Sleman, DIY.

Gambaran Indeks Eritrosit Pada Anak Jalanan Di Kota Yogyakarta Hieronymus Rayi Prasetya; Titah Dewi Rahadian
Journal of Health (JoH) Vol 6 No 1 (2019): Journal of Health - Januari 2019
Publisher : LPPM STIKES Guna Bangsa

The transportation sector has proven to be one of the causes of lead pollution in the air. Lead pollution is a result of vehicle fuel use. Lead is a metal that is very dangerous because it can accumulate in the body resulting in reduced production of erythrocytes and erythrocyte morphological abnormalities. Buskers have a risk of red cell Indices abnormalities due to exposure to vehicle exhaust gas every day. Buskers in the city of Yogyakarta carry out activities (busking) every day around trafficlight so that it will increase the risk of respiratory poisoning. The study was conducted by giving questionnaires to 32 respondents and the red cell Indices examination was performed using a hematology analyzer. The results showed that the average value of red cell Indices based on sex, age, duration of work, alcohol and cigarette consumption habits were still within normal limits (normocytic normochromic). There are no red cell Indices abnormalities (size and color) in buskers in Yogayakarta City

Deteksi Filariasis Bancrofti dengan Filariasis Test Strip (FTS) dan Perilaku Pencegahan Gigitan Nyamuk di Desa Endemis Filariasis Amban Manokwari Papua Barat Nurlaili Farida Muhajir; Hieronymus Rayi Prasetya; Novyan Lusiyana; Desto Arisandi
BALABA: JURNAL LITBANG PENGENDALIAN PENYAKIT BERSUMBER BINATANG BANJARNEGARA Volume 16 Nomor 1 Juni 2020
Publisher : Balai Penelitian dan Pengembangan Kesehatan Banjarnegara Badan Litbangkes Kemenkes RI

Mass Drug Administration (MDA) of filariasis in Amban, the endemic area of filariasis, was carried out since 2016. The treatment target of the filariasis area is the area with the microfilaria rate (Mf rate ≥ 1%). The purposes of this study were to detect Wuchereria bancrofti antigens after 3 years of mass treatment and to find out the environmental characteristic and preventive behavior from mosquito bites in Amban the endemic filariasis village Manokwari, West Papua. This observational analytic study was conducted in Amban by examining the subject with the AlereTM Filariasis Strip Test (FTS) and questionnaires collection on 56 research subjects. Respondents were taken capillary blood and followed by tracking environmental and behavioral data of questionnaires. The data obtained was performed by bivariate analysis. The result showed that 56 respondents were negative for W. bancrofti filarial antigen (Mf rate 0%). Research subjects were dominated by the age range of adults, moderate education, and unemployment. The subjects living environment 58.9% far from the garden and 57.1% far from standing water. Preventive behavior from mosquito bites showed that 44.1% use wire ventilation; 44.6% use repellent; 19.6% do not do an outdoor activity at the night; 39.3% use mosquito nets; 28.6% do not do habits of hanging clothes. The level of education was related to the behavior to use repellent (p = 0.025).

HUBUNGAN TIMBAL DARAH TERHADAP KELAINAN SEL DARAH PADA ANAK JALANAN DI KOTA YOGYAKARTA Hieronymus Rayi Prasetya
Meditory : The Journal of Medical Laboratory Vol 9, No 1 (2021): Meditory, Volume 9, No 1, Juni Tahun 2021
Publisher : Jurusan Analisis Kesehatan, Poltekkes Kemenkes Denpasar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33992/m.v9i1.1291

Introduction : Increasing ownership of motorized vehicles will be accompanied by increased use of fuel followed by high air pollution (lead). Pb can cause erythrocyte hemolysis and inhibit the formation of hemoglobin. This causes a decrease in the life span of erythrocytes and increases the fragility of erythrocyte membranes. Lead poisoning in the blood is characterized by basophilic stippling in erythocytes. Street musicians who do their work on the edge of the road (traffic light) are a group of populations that are susceptible to Pb poisoning, due to exposure to vehicle fumes every day as well as low knowledge about health conditions and the use of personal protective equipment (masks).Aims : This study aims to determine the effect of lead exposure on quantity (amount) and quality of blood cells (morphology).Method : The study was conducted by survey method, questionnaire and laboratory examination (blood lead, complete blood count, blood cell morphology). The data obtained were 32 samples analyzed using Spearman nonparametric with a confidence level of 95%.Results : The results of blood lead examination obtained 100% of respondents had normal lead levels (100 µg / L). The results also showed no association between blood lead with hemoglobin, erythrocytes, platelets, leukocytes, hematocrit, neutrophils, lymphocytes, monocytes, eosinophils, and basophils. Based on the results of morphological examination of erythrocytes, it shows that all respondents did not experience erythrocyte size, color and shape abnormalities and found no basophilic stippling.Conclutions : There is no relationship between blood lead and the quantity of blood cells. Morphological examination of blood cells found no cell abnormalities and found no basophilic stippling. Despite being exposed to vehicle fumes every day, respondents of street children did not experience lead poisoning and did not experience blood cell disorders.

Gambaran Populasi Golongan Darah Subgroup A (A1, A2) di PMI Kulon Progo Hieronymus Rayi Prasetya; Bambang Heru Budianto; Hernayanti Hernayanti
Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Vol 34, No 1 (2017)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.mib.2017.34.1.375

Subgroup A1 and A2 are the most important in the blood group A. Subgroup A1 has the A antigen more than A2 subgroup, the A2 subgroup can cause misidentification of blood group due to poor A antigen and genetic variation possessed. Misidentification of the blood group will increase the risk of transfusion reactions. This research aims to describe the A1 and A2 subgroup population in Kulon Progo district. This study was conducted with a cross sectional sampling technique. The sample in this study were taken from donors of blood group A in Kulon Progo Red Cross. Identification of A1 and A2 subgroup is done by using lectin (Dolichos biflorus extract). The result of the examination of 53 samples showed that 96,2% was A1 subgroup and 3,8% was A2 subgroup.Key words : Subgroup A1, Subgroup A2, Population, Kulon Progo

PENGGUNAAN SIX SIGMA PADA PEMERIKSAAN JUMLAH LEUKOSIT DI RSUD PANEMBAHAN SENOPATI BANTUL Hieronymus Rayi Prasetya; Nurlaili Farida Muhajir; Magdalena Putri Iriyanti Dumatubun
Journal of Indonesian Medical Laboratory and Science Vol 2 No 2: Oktober 2021
Publisher : Asosiasi Institusi Pendidikan Tinggi Teknologi Laboratorium Medik Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.53699/joimedlabs.v2i2.72

Internal quality assurance is a prevention and control activity that must be carried out by the laboratory continuously and covers all aspects of laboratory examination parameters. Hematology examination in the laboratory is carried out using a Hematology analyzer, but this tool has limitations, one of which is that it can make leukocyte count reading errors. In order for the results of the tool to be reliable, it is necessary to carry out quality control on the hematology analyzer. The use of Westgard multirule is commonly used in laboratories, but the application of six sigma is still very rarely used, especially in the field of hematology. This research aims to know the internal quality control of the analytical stage of the Hematology analyzer for the leukocyte count based on the analysis of Westgard and Six sigma. This type of research is descriptive research. The sample in this study is the control value data for the examination of the leukocyte count for 1 month at Panembahan Senopati Hospital. The data were analyzed using the Westgard rules and Six sigma. At low level, 13s deviation (random error) is obtained. At the normal level, there is a deviation of 12s (warning). At high level 12s deviation is obtained (warning). The sigma scale at all control levels shows a scale above 6. Analysis based on six sigma for leukocyte count showed an average of 7.16 sigma which indicates that leukocyte examination using a hematology analyzer has an accuracy of 99.9%.

Co-Authors Bambang Heru Budianto Desto Arisandi Diani Mentari Hernayanti Hernayanti Magdalena Putri Iriyanti Dumatubun Maria Elisabeth Enjel Naur Maria Irena Dentri Novita Sari Novyan Lusiyana Nurlaili Farida Muhajir Nurlaili Farida Muhajir Relita Pebrina Sistiyono Sistiyono Sistiyono Sistiyono Syaqina Rassajati Titah Dewi Rahadian Visensa Gerosa Naer Yuliana Prasetyaningsih

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