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All Journal ASPIRATOR Prosiding JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Biosaintifika: Journal of Biology & Biology Education Scripta Biologica BioEksakta : Jurnal Ilmiah Biologi Unsoed Prosiding SNPBS (Seminar Nasional Pendidikan Biologi dan Saintek)
Bambang Heru Budianto
Universitas Jenderal Soedirman
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Education Environmental Science Immunology & microbiology Medicine & Pharmacology Veterinary

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AUGMENTASITUNGAU PREDATOR FAMILIA PHYTOSEIIDAE RESISTEN TEMPERATUR SEBAGAI UPAYA KONSERVASI BIODIVERSITAS BERKELANJUTAN DALAM PENGENDALIAN Tetranychus urticae Basuki, Edi; Budianto, Bambang Heru
Prosiding Vol 3, No 1 (2012)
Publisher : Prosiding

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Tujuan dari penelitian ini adalah untuk mengetahui karakter padi gogo toleran kekeringan dengan daya hasil tinggi pada kondisi kadar air tanah rendah pada sistem tanam intercrops dengan rumput. Penelitian dilakukan di lahan tadah hujan Desa Banjaranyar dengan menggunakan Rancangan Petak Terbagi diulang tiga kali. Petak utama terdiri atas tanpa rumput, rumput gajah dan sereh serta anak petak terdiri atas vartietas Situ Patenggang, Kalimutu, Danau Gaung, Jatiluhur dan Cisokan. Pada kondisi kadar air tanah rendah (
Ecological Factors Determining Abundance of Parasitic Mites on Aedes spp. Larvae Firmansyah, Nurhadi Eko; Soviana, Susi; Budianto, Bambang Heru
Biosaintifika: Journal of Biology & Biology Education Vol 9, No 3 (2017): December 2017
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v9i3.11458

Abstract

Ability to infestation and abundance of parasitic mites in Aedes spp. larvae cannot be separated from the influence of various factors. Ecological factors have been suggested to play a role determine the presence of parasitic mites that under certain conditions become a key factor in determining the abundance of parasitic mites on Aedes spp. larvae. The aim of this study to determine the ecological factors affect the abundance of parasitic mites on Aedes spp. larvae in Bogor Regency. Capturing of Aedes spp. larvae was performed directly on the habitats found in indoor and outdoor. Capturing mites in the body of Aedes spp. larvae was performed using insect forceps. Ecological factors measured were dissolved oxygen (DO), pH, temperature, and total dissolved solid (TDS). The influence of ecological factors was analyzed using regression and correlation analysis. The result of mite identification has been obtained three species of mites that are Halacarus sp., Histiostoma sp., and Hydrozetes sp. The result indicated that total dissolved solid (TDS) and temperature was the factors that determined the abundance of mites. The factors of pH, and dissolved oxygen (DO) did not determine the abundance of parasitic mites of Aedes spp. larvae. The research result can be further developed as a new alternative to Dengue Hemorraghic Fever control and provide information on parasitic mites that infest Aedes spp. larvae. In addition, this results become an early step in controlling of Aedes spp. strategy platform by the parasitic mites.
Studi Toksisitas: Ekstrak Metanol Bonggol Pisang Ambon (Musa acuminata L. cv. Gros Michel) terhadap Aedes aegypti (Diptera: Culcidae) Siskha Noor komala; Bambang Heru Budianto; Edi Basuki
ASPIRATOR - Journal of Vector-borne Disease Studies Vol 10 No 2 (2018): Jurnal Aspirator Volume 10 Nomor 2 2018
Publisher : Loka Litbang Kesehatan Pangandaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (375.156 KB) | DOI: 10.22435/asp.v10i2.217

Abstract

Aedes aegypti mosquito is the main vector of dengue virus causing dengue hemorrhagic fever (DHF) in Indonesia. The main preventive action is to control the presence of Ae. aegypti mosquito. Banana plants are known to contain secondary metabolite compounds acting as a natural insecticide, including the hump part. This research was conducted to evaluate to toxicity of hump of Ambon banana extract (Musa acuminata L. cv. Gros Michel) to dengue vector Ae. aegypti mosquito. The research used experimental method, the treatment concentration was 0.001; 0.01; 0.1; 1; 10; 100 and 1000 ppm of Ambon banana methanol extract and 0 ppm concentration as a control, each with three replications. The variables observed were individual deaths at every stage of development and morphological damage. The data obtained were analyzed using the analysis of variety and Duncan test with 95% confidence level. In addition, probit analysis was used to determine the value of LC50. The results showed that the study of toxicity indicates that in the further stage of development, the toxicity of methanol extract from Ambon banana hump was less toxic. Toxicity of Ambon banana hump methanol extract was highest in egg with LC50 value of 314,852 ppm. The methanol extract of banana Ambon has the morphological destructive activity in all development stages of Ae. aegypti.
Kemampuan Predasi Tungau Predator Amblyseius sp. Resisten Temperatur terhadap Tetranychus urticae Budianto Heru Bambang; Edi Basuki
Jurnal Hama dan Penyakit Tumbuhan Tropika Vol. 13 No. 1 (2013): MARET, JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA
Publisher : Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (101.384 KB) | DOI: 10.23960/j.hptt.11335-41

Abstract

he predation ability of temperature resistant Amblyseius sp. to Tetranychus urticae. Global warming and climate changes have caused great mortality of the predatory mites Amblyseius sp. that disrupts the natural control of Tetranychus urticae. In contrast, the low humidity and high temperature led to an increase in the population of T. urticae. The purposes of this study were to determine the effect of temperature on population of the predatory mites Amblyseius sp. and to determine the predation ability of temperature resistant Amblyseius sp. on T. urticae. The selection of Amblyseius sp. was conducted at temperatures of 15, 20, 25, 30 and 35 0C. The parental predatory mite Amblyseius sp., and subsequent descendants were exposed to the temperature range until the values of the LT50 fiducial limits (FL) of a certain generation did not overlap with that of the previous one. The effectiveness of Amblyseius sp. predation ability was studied using an experimental method i.e. completely randomized design with four treatments and ten replications . For this purpose, each stage of T. urticae, i.e. egg, larvae, nymph and adult was set as treatment and was given to any type of temperature resistant predatory mite Amblyseius sp. The results showed that by using the temperature gradient, we managed to select temperature-resistant individuals from their population. These individuals formed a population that was resistant to temperature of up to 33.3oC without losing their predatory capacity, especially on the egg stage of T.urticae.
KEMAMPUAN REPRODUKSI TUNGAU PREDATOR FAMILI PHYTOSEIIDAE PADA BERBAGAI KEPADATAN TETRANYCHUS URTICAE DAN POLEN TANAMAN DI SEKITAR TANAMAN SINGKONG (MANIHOT ESCULENTA CRANTZ) Bambang Heru Budianto; Achmad Munadjat
Jurnal Hama dan Penyakit Tumbuhan Tropika Vol. 12 No. 2 (2012): SEPTEMBER, JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA
Publisher : Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (334.636 KB) | DOI: 10.23960/j.hptt.212129-137

Abstract

The predatory mite of Phytoseiidae family have extensive cruising range that is not only found in cassava, but also a variety of weeds in cassava plants, such as Ageratum conyzoides and Cyperus cyperus. Extensive cruising range is also well explain the survival rate of the Phytoseiidae family at the time of the density of T. urticae decreased. The research objective is to determine the development period, fecundity and survival rate of Phytoseiidae predatory mite populations at different relative densities T. urticae and pollen. The research method used is an experimental laboratory. The procedure include the provision of A. conyzoides and C. rotundus pollen, the multiplication of predatory mite families Phytoseiidae, the determination of the development period, fecundity and survival rate of predatory mite families Phytoseiidae. The results showed that the length of time the development of predatory mites Amblyseius sp. and Phytoseius sp. faster one day of feeding on egg T. urticae feeding compared to the two types of pollen. Predatory mites survival rate Phytoseius sp. higher than the predatory mites Amblyseius sp. eggs fed T. urticae and both types of pollen. Similarly, the fecundity Phytoseius sp. higher than the mites Amblyseius sp. either fed eggs T. urticae as well as to two types of pollen.
Life Table Lalat Buah (Drosophila melanogaster) yang Didedahkan pada Konsentrasi Subletal Sipermetrin aan wahyudin; Bambang Heru Budianto; Edi Basuki
BioEksakta : Jurnal Ilmiah Biologi Unsoed Vol 3 No 3 (2021): BioEksakta
Publisher : Fakultas Biologi Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.bioe.2021.3.3.4434

Abstract

Drosophila melanogaster or commonly known as the fruit fly, is a type of insect found in rotting fruits or vegetables and plants material around the world with a wide distribution. This study aims at determining the LC50 value of eggs, larvae, pupae and the reproductive potential of adult fruit flies (Drosophila melanogaster) exposed to cypermethrin. The method used was an experiment using a completely randomized design (CRD). The data of mortality from toxicity tests were analyzed by ANOVA and Probit test using the SPSS application, while data on reproductive potential parameters were analyzed using the life table method using Ms. Excel. The results of this study showed that the LC50 value of cypermethrin was different at each development stage. The LC50 value from the lowest to the highest successively starts at the egg development stage, which was 1654,824 ppm, then at the egg stage was 3067,192 ppm, the imago stage was 3155,848 ppm and the pupae stage was 3755,014 ppm. The reproductive potential and population rate of D. melanogaster decreased significantly with increasing concentration, lower than the control. The best concentration that was able to reduce the value of the net reproduction rate (R0) and the ability of the population to multiply (λ) was 266.6 ppm with an average value of 0.22 and 0.86, respectively. Further, the best concentration in reducing the average life period (T) and the population reproductive potential (rm) were a concentration of 250.0 ppm with an average value of each parameter of 9.64 and -0.03.
Kelimpahan Tungau Predator pada Tanaman Melati Gambir (Jasminum officinale) di Desa Cipawon, Bukateja, Purbalingga, Jawa Tengah Ika Rakhmayani; Bambang Heru Budianto; Trisnowati Budi Ambarningrum
BioEksakta : Jurnal Ilmiah Biologi Unsoed Vol 3 No 1 (2021): BioEksakta
Publisher : Fakultas Biologi Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.bioe.2020.2.3.3927

Abstract

The Gambier Jasmine plant (Jasminum officinale) is located in Bukateja District, Cipawon Village, Purbalingga, Central Java is a commodity with high economic value, its use a tea fragrance, cosmetics, perfumes dan others. One of the cause of decreased productivity of Gambier Jasmine is ude to the presence of pets, namely mites. The pest mite population is controlled by predatory mites. Therefore, this study aimed to determne the types of predatory mites and determine the abundance of predatory mites in Gambier Jasmine (J. officinale) in Cipawon Village, Purbalingga, Central Java. This research used survey method with purposive sampling technique. There are nine sampling points, eight pointa on the outer edge and one point in the middle of plantation. Each sampling is represented by three trees. The sample in the form of Gambier Jasmine Leaves came form the lowest ten leaf stalks in one tree at each sampling point. The number of leaf samples obtained was 270 leaf samples. Each sampling point was taken three times with one week intervals. The variable observed include the number of individuals and the number of species of predatory mites on the Gambier Jasmine (J. officinale) and the parameters calculated were leaf area, leaf sitting angle, length and density of trichomes, temperature, humidity, asa well as rainfall. The data obtained were analyzed by analysis of variance (ANOVA) with an error rate of 20%. The result of the identification of predatory mites on the Gambier Jasmine plant were obtained by two species, namely Amblyseius largoensis and Phytoseius amba. A. largoensis as many as 76 individuals with an abundance percentage of 61,8% and P. amba as many as 47 individuals with an abundance percentage of 38,2%. The abundance of predatory mites A. largoensis and P. amba is classified as moderate because predators are easy to find, every three leaves there is one predator. Key words: abundance., Cipawon., Gambier Jasmine., predatory mites.
Prevalensi dan Intensitas Tungau Parasit pada Kucing Peliharaan yang Diperiksakan di beberapa Klinik Hewan Purwokerto Amelia Nurma Hidayah; Bambang Heru Budianto; Hery Pratiknyo
BioEksakta : Jurnal Ilmiah Biologi Unsoed Vol 3 No 2 (2021): BioEksakta
Publisher : Fakultas Biologi Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.bioe.2021.3.2.4564

Abstract

This study aimed to determine the prevalence and intensity of parasitic mites in domestic cats at several veterinary clinics in Purwokerto. This research was conducted by cross sectional method using random sampling technique at three veterinary clinics in Purwokerto City. The identification of parasitic mites was analyzed descriptively, the data on the mites found were then analyzed using the prevalence and intensity formulas. The prevalence and intensity figures obtained were then compared with the prevalence and intensity category tables. The results of the study reported that the total prevalence of parasitic mites was 85%, which was included in the category of prevalence usually with moderate infestation, with a total intensity of parasitic mites of 196.7 which included in the category of very severe intensity. Species of parasitic mites found include Otodectes cynotis, Notoedres cati and Lynxacarus radovskyi.
DISTRIBUSI GEOGRAFIS TUNGAU PARASIT NYAMUK Aedes sp. DI DAERAH ENDEMIS DEMAM BERDARAH DENGUE DI KABUPATEN BANJARNEGARA Arthur Shepta Laksono; Bambang Heru Budianto; Endang Ariyani Setyowati
Scripta Biologica Vol 1, No 2 (2014)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (699.063 KB) | DOI: 10.20884/1.sb.2014.1.2.436

Abstract

Parasitic mites are known as the natural enemy of Aedes sp. that can potentially infect any life stages of the Aedes sp. The parasitic mites’ ability to infect Aedes sp. influence the distribution pattern of parasitic mites. The widespread distribution of Aedes sp. is expected to affect the distribution of parasitic mites. The aims of this study were to determine the taxonomic family of parasitic mites that infected Aedes sp. larvae and to determine the geographical distribution patterns of parasitic mites of Aedes sp. in the endemic area of Dengue Hemorrhagic Fever (DHF) in Banjarnegara District. This study used larvae of Aedes sp. taken from the endemic area of DHF in Banjarnegara District. The study sites were in the Parakancanggah Village, Kutabanjar Village, Krandegan Village, and Sokanandi Village. This study used survey method with purposive sampling technique. Variable observed in this research was the distribution pattern of the parasitic mite of Aedes sp. larvae. Parameter observed included the family and the individual number of parasitic mites on each mosquito larvae, the average value, and the variance. Data were analyzed using mean value and the variance so that the distribution pattern can be determined. The level of parasitic mites’ distribution was analyzed using K' index negative binomial distribution. Results showed that 30 individual of parasitic mite were found from 1429 samples of examined Aedes sp. larvae. The identification result were five families of parasitic mites: Pionidae, Histiostomatidae, Hydryphantidae, Hydrachnidae, and Arrenuridae. The distribution pattern of the parasitic mites was regular, and the highest value of K’ index negative binomial distribution was 1,3225, in the Krandegan Village.
FAKTOR RESIKO GANGGUAN FUNGSI GINJAL PADA PEKERJA BENGKEL LAS DI KOTA PURWOKERTO Anjar Astuti Ferdhiani; Hernayanti Hernayanti; Bambang Heru Budianto
Scripta Biologica Vol 5, No 2 (2018)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

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Abstract

Welding was one of the industry in Purwokerto City. One of the exhaust particulate material from the welding process, this will be the potential occurrence of exposure to workers and caused renal dysfunction. Several risk factors have contributed in caused kidney difunction of workers. This study aims to determine the levels of cadmium metal (Cd), chromium (Cr), lead (Pb) with creatinine and Glomerulus Filtration Rate (GFR). The correlation of risk factors for renal dysfunction with creatinine and GFR. The risk factors that play a major role in caused kidney dysfunction. The method used is cross sectional. Sampling using purposive sampling with giving inform consent to the respondent. Provision of questionnaires and blood sampling as much as 3 ml according to ethical clearens. Measurement of metal content of Cd, Cr and Pb; serum creatinine and LFG levels. Number of respondents 30 people with 95% confidence level. The results showed that levels of Cr, Cd and Pb were 0.049 + 0.12 ppm; 1,029 + 2.38 ppm; 4,933+ 11,66 ppm. Cr levels are still within normal limits with levels but Cd and Pb exceeding the normal threshold. Creatinine levels of 1.867 + 0.446 mg / dl showed higher than normal. Minimum of GFR decrease in welding workers was 44 mL / min / 1.73 m2. Risk factors significantly correlated with creatinine and GFR was levels of Pb (p <0.05). Risk factors that play a major role in caused kidney dysfunction in welding workers in Purwokerto city is Pb content with 12.9% percentage effect on serum creatinine level and 9% effect on Glomerulus Filtration Rate.
Co-Authors aan wahyudin Achmad Munadjat Amelia Nurma Hidayah Anjar Astuti Ferdhiani Arthur Shepta Laksono Edi Basuki Edi Basuki Edi Basuki Edi Basuki Firmansyah, Nurhadi Eko Haryanto, Trisno Heri Wibowo HERNAYANTI HERNAYANTI Hernayanti Hernayanti Hery Pratiknyo Hery Pratiknyo Hieronymus Rayi Prasetya Ika Rakhmayani Imam Widhiono Lilik Setyowatiningsih Muhammad Samsi Nurhadi Eko Firmansyah R Rokhmani Retno Widiastuti Rheza Paleva Wijaya Setyawati, Endang Ariyani Shintya Nitra Nirwani Siskha Noor komala Susi Soviana Trisnowati Budi Ambarningrum