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All Journal AGRISAINS AL KAUNIYAH Proceedings of KNASTIK Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati JURNAL PEMULIAAN TANAMAN HUTAN Biology, Medicine, & Natural Product Chemistry Pro-Life Edumatsains Jurnal Inovasi Hasil Pengabdian Masyarakat (JIPEMAS) Jurnal Pembelajaran dan Biologi Nukleus Sciscitatio : Journal for Biological Science Jurnal Agrohut
Aniek Prasetyaningsih
Program Studi Biologi, Fakultas Bioteknologi, Universitas Kristen Duta Wacana
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Education Engineering Environmental Science Immunology & microbiology Languange, Linguistic, Communication & Media Materials Science & Nanotechnology Mathematics Medicine & Pharmacology Physics Public Health Other

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KEANEKARAGAMAN DAN BIOAKTIVITAS SENYAWA AKTIF MAKROALGA PANTAI WEDIOMBO KABUPATEN GUNUNG KIDUL Prasetyaningsih, Aniek; Rahardjo, Djoko
AgriSains Vol 17, No 2 (2016)
Publisher : FAPETKAN UNTAD

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (336.341 KB)

Abstract

Research of macroalga diversity and bioactivity was conducted from February to September 2016 at Wediombo beach, Gunung Kidul district. The used sampling method was “metode jelajah” and the environmental parameters measured include temperature, salinity, depth, pH, turbidity, dissolved oxygen (DO), and concentration of nitrate and phosphate. Identification was based on morphological characteristics. Extraction sample was stratified using ethanol and ethyl acetate. Method of HTS (High Throughput Screening) was applied to test the potential of active compounds. Antimicrobial Test was conducted with cell growth indicators (MTT), while the indicator DPPH was used for the antioxidant test (80mg / 100ml), and TLC and GC-MS for profiling the active compound. Seventeen species macroalgal were found at the shore of Wediombo, includes: Rhodophyta (7 species), Phaeophyta (5 species) and Chlorophyta (5 species) of which were dominated by macroalgal on coral reefs. Nine macroalgae found to have antioxidant activity (Acrocystis nana, Caulerpa sp., Chaetomorpha crassa, Dictyota sp., Gracilaria canaliculata, Gelidiella acerosa, Sargassum sp., Turbinaria sp. and Ulva lactuca) and 3 macroalgae (Boergesenia forbesii, Halymenia maculata and Palisada concreta ) did not possess the activity. Macroalgae which were tested for microbial activity, generally had antimicrobial against C. albicans and Shigella sonnei except E. coli. Results of TLC indicated that all of macroalgal had active compounds: terpenoid, glucose, flavonoids, phenols and amino acids but only few of them contained saponin, based on GC-MS test, Acrocystis nana, Zanardini was found to contain 1-Octadecyne (17.78%), Octadecanal (17, 83%) Dicholesteryl succinate (37.53%) and Cholestan-3-one, 4-methyl (26.86%) had the potential to be antimicrobial and antioxidant as well as bioenergy sources. Key words : Macroalga, High-Troughput Screening,  metabolites profiling
ANALISIS PERBANDINGAN ALGORITMA PENCARIAN STRING BOYER-MOORE DAN KNUTH-MORRIS-PRATT PADA PENCARIAN ENZIM RESTRIKSI Waty, Wulan Krista; Raharjo, Willy Sudiarto; Prasetyaningsih, Aniek
Proceedings of KNASTIK 2010
Publisher : Duta Wacana Christian University

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Abstract

Pencocokan string merupakan masalah yang hampir ditemukan dalam kegiatan sehari-hari, yaitu berhubungan dengan  text-processing. Hal ini dimulai dari permasalahan sederhana sampai pada permasalahan yang kompleks. Pencocokan string fokus pada pencarian satu atau lebih pola, semua kehadiran pola (lebih umum disebut pattern) dalam sebuah teks. Masalah dalam algoritma pencarian adalah mencari kata yang mempunyai panjang m, yang disebut pattern, dalam sebuah teks dengan panjang n. Algoritma yang digunakan yaitu Boyer-Moore dan Knuth-Morris-Pratt. Boyer-moore mencocokan pola dari kanan ke kiri sedangkan Knuth-Morris-Pratt mencocokan pola dari kiri ke kanan. Dalam makalah ini akan dijelaskan mengenai cara kerja algoritma Boyer-Moore dan algoritma Knuth-Morris-Pratt dalam pencarian enzim restriksi pada sequence DNA serta analisis running time dan turn around time yang dimiliki kedua algoritma tersebut.
Potency of Citrus reticulata Peel Extract as Active Compound of NonAlcohol Based Gel Hand Sanitizer Santoso, Julyana Dika; Prasetyaningsih, Aniek; Madyaningrana, Kukuh
SCISCITATIO Vol 1 No 2 (2020): Volume 1, Number 2, July 2020
Publisher : Universitas Kristen Duta Wacana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21460/sciscitatio.2020.12.32

Abstract

Hand sanitizer is nowadays known as a part of one’s personal hygiene kit because of its practical use andeffectivity against skin microbes, such as Staphylococcus aureus. The common commercial hand sanitizers are madefrom alcohol which may have negative side effect like skin irritation. Utilization of active compounds from a plantthat has antibacterial compounds might be applied to the substitution of alcohol in the formulation of hand sanitizer.In this study, phytochemical compounds from Citrus reticulata and Citrus aurantifolia peel extracts were testedqualitatively and their antibacterial acitivity on Staphylococcus aureus were tested using disk diffusion method. Theresults showed that Citrus reticulata peel extract at the concentration of 70% was the most effective concentration ininhibiting Staphylococcus aureus. This certain concentration of Citrus reticulata peel extract was then chosen in the gelhand sanitizer formulation. Application of gel hand sanitizer with Citrus reticulata peel extract as its active compoundon the hands of respondents were also observed to inhibit bacterial growth. The use of peel extracts from Citrusspp, especially from Citrus reticulata might be potential in the formulation of non-alcohol based gel hand sanitizer.
Utilization of Used Cardboard and Vegetable Waste as Growth Media for White Oyster Mushroom (Pleurotus ostreatus) Lavenia, Jovita; Prasetyaningsih, Aniek; Ariestanti, Catarina Aprilia
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 6, No 2 (2021): June 2021
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24002/biota.v6i2.4278

Abstract

White oyster mushroom (Pleurotus ostreatus) is a highly nutritious food with simple cultivation process using sawdust as its main substrate. Nowadays, availability of sawdust become a problem for some mushroom farmers. Cardboard and vegetable wastes are easily found and difficult to maintain. Based on their characteristics, those wastes can be used as mushroom growth media. This study aims to determine the effect of cardboard and vegetable waste on mushroom growth and the best media composition to obtain maximum productivity. Those wastes are used as the main substrate in eleven composition variations with complete randomized design (CRD) analysis method using One-Way ANOVA and simple linear regression statistical test. The results showed, variations in media composition affect the condition of the media which then influence the growth of P. ostreatus. Maximum productivity was found in media composition XI with mycelium growth rate up to 3.91 cm/day, media IX with the biological efficiency up to 45.38%, and media X with the highest total yield up to 97.28 grams. From this study it can be concluded that cardboard and vegetable waste can be an alternative growth substrate for P. ostreatus in urban areas with a maximum concentration of vegetable waste no more than 30% by weight of the media used.
Antibacterial Activity of Lime Peel and Lemongrass Extract as Active Ingredients for Spray Hand Sanitizer Djima, Elza Gitaa Eirene Djima; Prasetyaningsih, Aniek; Madyaningrana, Kukuh
SCISCITATIO Vol. 2 No. 1 (2021): Volume 2, Number 1, Januari 2021
Publisher : Universitas Kristen Duta Wacana

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Abstract

Hand sanitizers are widely used as an alternative way to maintain hand hygiene from the presence ofpathogenic bacteria, such as Staphylococcus aureus. In general, excessive use of alcohol as the main ingredientin hand sanitizers might cause skin irritation. Utilizing antibacterial activity of plants as a component in handsanitizer could be used as a substitution for alcohol. The aim of this study was to determine the potentialantibacterial activity of lime peel and lemongrass extracts as active ingredients for hand sanitizer. Methodused in this research included extraction using maceration, qualitative phytochemical test, antibacterialassay, and formulation of spray hand sanitizer and quality test of spray hand sanitizer. In this study alkaloid,flavonoid, saponin, tannin and terpenoid were found as phytochemical content of lime peel, while lemongrassextract contain fla vonoid, tanin, saponin and steroid. Compared to lemongrass extract, lime peel extract withconcentration 40%showed the optimum inhibition zone of S. aureus and chosen as active ingredient in sprayhand sanitizer formulation. Formulated spray hand sanitizer with lime peel extract was able to inhibit thegrowth of S. aureus bacteria on hand palm.
Pemanfaatan Senyawa Metabolit Sekunder Ekstrak Eter Diadema setosum dari Pantai Kukup dan Pantai Sundak Gunungkidul sebagai Antiinflamasi Tiffany, Yollanda; Prasetyaningsih, Aniek; Aditiyarini, Dwi
SCISCITATIO Vol. 2 No. 2 (2021): Volume 2, Number 2, Juli 2021
Publisher : Universitas Kristen Duta Wacana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21460/sciscitatio.2021.22.68

Abstract

Bulu babi atau landak laut merupakan hewan laut yang tergolong dalam kelas Echinoidea. Kelompok hewan dari kelas Echinoidea mempunyai kemampuan menghasilkan metabolit sekunder yang memiliki efek farmakologis seperti antibakteri, antitumor dan kanker, antioksidan, dan antiinflamasi. Salah satu metabolit sekunder dari yang dihasilkan oleh hewan dari kelompok Echinoidea adalah Ovothiol-A yang berpotensi sebagai antiinflamasi. Penelitian ini bertujuan untuk mempelajari metabolit sekunder dari Diadema setosum yang berasal dari Pantai Selatan Gunungkidul dan studi potensinya sebagai antiinflamasi. Hasil uji KLT dan FTIR menunjukkan bahwa spesies Diadema setosum sebagai salah satu anggota Echinoidea diduga mengandung senyawa Ovothiol-A pada bagian organ maupun cangkangnya. Senyawa diduga sebagai Ovothiol-A yang dihasilkan oleh D. setosum memiliki kemampuan antiinflamasi yang ditunjukkan oleh penyempitan luka pada mencit yang diberi perlakuan ekstrak bagian organ (100%) D.setosum dengan rata-rata penyempitan luka 0,44±0,256 mm/hari. Hasil dari penelitian ini menunjukkan bahwa ekstrak D. setosum memiliki senyawa Ovothiol-A yang dapat berfungsi sebagai antiinflamasi. Ekstrak D. setosum bagian organ memiliki kemampuan antiinflamasi yang lebih baik.
Keragaman Genetik dan Hubungan Kekerabatan pada Tiga Jenis Aquilatria Menggunakan Penanda RAPD AYPBC Widyatmoko; Elsih Dian Ariningsih; Aniek Prasetyaningsih
Jurnal Pemuliaan Tanaman Hutan Vol 5, No 3 (2011): Jurnal Pemuliaan Tanaman Hutan
Publisher : Center for Forest Biotechnology and Tree Improvement (CFBTI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20886/jpth.2011.5.3.139-148

Abstract

Gaharu is an imprortant species of non wood forest product of Aquilaria spp with hight economic value. The natural stand has been exploited very intensive. The aim of the study were to investigate level of Aquilaria (namely A. malaccensis, a. beccariana and A. microcarpa) in order to provide genetic information for developing conservation strategy of the species. Aamples were colleted from 7 populations and analyzed using 26 RAPD primers. Total of 84 polymorpic loci have been obtained from these primers. Mean of genetic diversity within population indicated by A. malaccensis Muara Bungo population. A. malaccensis Berau population has the lowest genetic diversity. The highest genetic distance between populations was between A. micricarpa samboja and A. beccariana Berau. In contrast, genetic distance between A. Microcarpa Bearu and A. malaccensis Berau was the the lowest (0.017). based on Cluster analysis, The seven populations were divided into 2 groups. The first group consisted of 2 populations of A. malaccensis and A. microcarpa from Kalimantan.
POTENSI BIOAKARISIDA EKSTRAK DAUN MIMBA (Azadirachta Indica) DAN BUNGA CENGKEH (Syzygium Aromaticum) TERHADAP TUNGAU PENYEBAB PENYAKIT KREPES PADA JAMUR KUPING Meilani Apra; Aniek Prasetyaningsih; Kukuh Madyaningrana
EduMatSains : Jurnal Pendidikan, Matematika dan Sains Vol 5 No 2 (2021): Januari
Publisher : Fakultas Keguruan dan Ilmu Pendidikan, Universitas Kristen Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33541/edumatsains.v5i2.2241

Abstract

Wood ear mushrooms is commonly known as one of the cultivated edible mushrooms in Indonesia. The demand for this commodity is still steadily high and leads mushroom farmers to optimize its cultivation. For mushroom farmers in Yogyakarta, the prevalence of krepes disease which is caused by a group of species belongs to Acarina order is still high and leads to total loss of harvest. In order to diminish these Acarina species, the use of chemical acaricides is common because of their effectiveness in eradicating the pests despite their toxic effect to the environment. A strategy to provide environmental friendly acaricides which are extracted from plants is therefore important. This research aimed to study the potency of neem leaves (Azadirachta indica) and clove flowers (Eugenia caryophyllata) extracts as bioacarisides for Acarina species as the causal agent of krepes disease. Neem leaves and cloves flowers were extracted using maceration and soxhlet extraction methods, using 96% methanol as their solvents. Obtained crude extracts would then be subjected to phytochemical analysis using qualitative biochemical tests and GC-MS. The effectivity of extracts as bioacaricides were tested both in in vitro and field scale. In vitro tests showed that neem leaves extract and clove flower extract had LC50 value ​​of 0.22% and 0.48% respectively. Field scale test on mushroom baglogs showed that the efficacy of the neem leaves and clove flower to eradicate acarina were above 50%. Taken together, neem leaves and clove flower extracts are potential to be used as bioacaricides in mushroom farming.
POTENSI EKSTRAK DAUN BINAHONG (Anredera cordifolia (Ten.) Steenis) DAN MINYAK ATSIRI SERAI (Cymbopogon citratus (DC.) Stapf) SEBAGAI BAHAN AKTIF HAND SANITIZER SPRAY Adonia Felma Mosse; Aniek Prasetyaningsih; Dwi Adityarini
EduMatSains : Jurnal Pendidikan, Matematika dan Sains Vol 6 No 1 (2021): Juli
Publisher : Fakultas Keguruan dan Ilmu Pendidikan, Universitas Kristen Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33541/edumatsains.v6i1.2474

Abstract

Maintaining hand hygiene is an important habit to prevent disease caused by microorganisms and viruses. Handsanitizer is one of hygiene products as antibacterial and practical use. However, the use of alcohol as its main ingredient could irritate skin. Therefore, the alternative of alcohol is urgently found to prevent this side effect in same effectiveness. in this research, Binahong (Anredera cordifolia (Ten.) Steenis leaf extract and lemongrass oil (Cymbopogon citratus (DC.) Stapf) are used to replace alcohol as the main ingredient. This study aims to know the potential antibacterial activity of binahong leaf extract, lemongrass oil and its combination against S.aureus and its effectiveness as an active ingredient in hand sanitizer spray. Binahong extraction using the maceration method. The secondary metabolites of both extracts were identified by phytochemical assay. Then, their antibacterial activity was determined using a disk diffusion test for S. aureus. The organoleptic of hand sanitizer spray was observed. The highest antibacterial activity was obtained from the combination of binahong leaf extract and lemongrass oil 100% at ratio 1:1. This combination was used to formulate the hand sanitizer spray. The highest inhibitory activity of hand sanitizer spray was shown in the formulation with 3% active ingredients (extracts). This product had dark yellow in colour, distinctive aroma, and liquid texture and effectively suppresses bacterial growth on the hands. According to the results, binahong leaf extract and lemongrass oil are potentially used as active ingredients for natural hand sanitizer sprays.
UJI EFEKTIVITAS EPIKARPIUM BUAH NANGKA (artocarpus heterophyllus lamarck.) SEBAGAI SEDIAAN KRIM TABIR SURYA UV-B Tara Inastu Kandarp; Aniek Prasetyaningsih; Vinsa Cantya Prakasita
EduMatSains : Jurnal Pendidikan, Matematika dan Sains Vol 6 No 1 (2021): Juli
Publisher : Fakultas Keguruan dan Ilmu Pendidikan, Universitas Kristen Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33541/edumatsains.v6i1.2798

Abstract

Sunscreen is needed as protection against the skin from exposure to UV rays, because it will cause erythema, aging skin, blistering, and skin cancer, in skin that is not protected by sunscreen. This study aims to determine the in vitro and in vivo ability of epicarpium A. heterophyllus extract sunscreen cream preparations. This research was conducted in vitro and in vivo, beginning with the extraction process with the maceration method using 70% ethanol, identification of compounds in the extract, TLC, GC-MS, total flavonoids test, physical quality of cream preparations, SPF values ​​of extracts and creams, and in vivo sunscreen activity tests were conducted. The yield percentage produced from 450 grams of simplicia powder was 47.61%. The compounds in A. heterophyllus epicarpium extract are flavonoids, tannins, saponins, terpenoids, steroids, fatty acid groups, alcohol groups, and alkanes. Crude extract contains total flavonoids of 43.625 mgQE / g extract. The 75% concentration in the cream preparation had the highest SPF value of 8.173. The in vivo test used the MED (Minimal Erythema Dose) method of erythema scoring. A concentration of 75% gives the lowest MED value of 0.25. This research is expected to provide information on alternative uses of natural ingredients as the main ingredient of sunscreen so that the use of chemicals as the main ingredient will be reduced.
Co-Authors Adonia Felma Mosse Angelia Astria AYPBC Widyatmoko Catarina Aprilia Ariestanti Devi Sonti Sibarani Djima, Elza Gitaa Eirene Djima Djoko Rahardjo Djoko Rahardjo Dwi Aditiyarini Dwi Adityarini Elisabet Suharti Soleman Elsih Dian Ariningsih Kania Apenta Olisvelos Kisworo Kisworo Kukuh Madyaningrana Lavenia, Jovita Maria Grasela Kase Meilani Apra Nurlia Widayanti Ratih Restiani Santoso, Julyana Dika Seta Nurhayati Mularum Setya Harianto Tara Inastu Kandarp Tiffany, Yollanda Vinsa Cantya Prakasita Willy Sudiarto Raharjo Wulan Krista Waty