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All Journal Hemera Zoa Jurnal Sain Veteriner METAMORFOSA Journal of Biological Sciences AL KAUNIYAH Biosfer: Jurnal Tadris Biologi Prosiding Seminar Biologi Bioeksperimen: Jurnal Penelitian Biologi Edumatsains BERITA BIOLOGI BIOLINK (Jurnal Biologi Lingkungan, Industri, Kesehatan) BIOEDUSAINS: Jurnal Pendidikan Biologi dan Sains Sciscitatio : Journal for Biological Science
Vinsa Cantya Prakasita
Universitas Kristen Duta Wacana
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Education Environmental Science Immunology & microbiology Materials Science & Nanotechnology Mathematics Physics Social Sciences Veterinary Other

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Virulence Characteristic of Avian Pathogenic Escherichia coli (APEC) Isolates Adrenalin, Sruti Listra; Imanjati, Lynda Nugrahaning; Fauziah, Ima; Prakasita3, Vinsa Cantya; Widyarini, Sitarina; Wahyuni, Agnesia Endang Trihastuti
Jurnal Sain Veteriner Vol 38, No 1 (2020): April
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (842.327 KB) | DOI: 10.22146/jsv.57368

Abstract

Avian pathogenic Escherichia coli (APEC) merupakan penyebab kolibasilosis pada unggas, salah satu penyakit pernapasan yang menyebabkan permasalahan yang serius di industri perunggasan. APEC dapat mengakibatkan tingginya jumlah kematian dan angka afkir, penurunan produksi, dan tingginya biaya pengobatan. Manifestasi kolibasilosis yang biasa terjadi adalah airsacculitis, perihepatitis, dan perikarditis. Serotipe APEC yang banyak diidentifikasi di lapangan adalah O1K1, O2K1, dan O78K80. Embryo lethality assay (ELA) adalah salah satu metode yang dapat digunakan untuk mengetahui virulensi serotipe APEC. Tujuan dari penelitian ini adalah untuk mengetahui karakteristik virulensi isolat APEC berbagai serotipe. Lima isolat APEC serotipe O1K1, O2K1, O78K80, O157H7, dan unknown serotype digunakan untuk pengujian virulensi dengan menginokulasikan bakteri ke dalam cairan alantois telur ayam berembrio spesific pathogenic free (TAB SPF) umur 12 hari. Masingmasing serotipe dibutuhkan telur sebanyak 10_butir, dengan dosis bakteri 100-500 CFU/ 0.1 ml. Candling dilakukan setiap hari, sampai umur embrio 18 hari, untuk menentukan jumlah kematian dan perubahan lesi patologi. Setelah diamati selama 6 hari, persentase TAB yang mati setelah diinokulasi dengan APEC serotipe O1K1, O2K1, unknown serotype adalah 100% (10/10), serotipe O78K80 90% (9/10), dan serotipe O157H7 70% (70%). Seluruh ayam yang mati mengalami lesi patologi adanya hemoragi daerah kranial dan ektremitas. Seluruh isolat E. coli dalam penelitian ini memiliki virulensi tinggi. 
Bionomic of Anopheles sp. in Merauke District, Papua Rahardjo, Djoko; Prakasita, Vinsa Cantya; Pepiana, Marlen Aviati Sarah
SCISCITATIO Vol 1 No 1 (2020): Volume 1, Number 1, January 2020
Publisher : Universitas Kristen Duta Wacana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21460/sciscitatio.2020.11.20

Abstract

Malaria is known as an endemic disease that often causes death in Indonesia, especially in Papua. The malaria cases control in Papua has not been carried on based on data studies, therefore bionomic of Anopheles sp is important to be studied. Bionomics data are consisted of breeding places, resting places and feeding habits are from direct observation. Interviews and questionnaires were conducted to gain information about respondent behavior. Descriptive and qualitative data were then analyzed. The breeding places of Anopheles sp. were mostly found in swampy areas. Based on the feeding habit, the feeding activity of Anopheles sp. inside the house has only one biting peak at 23.00-02.00 WIT, while outside the house, biting peaks occurred at 21.00-22.00 WIT and 00.00-01.00 WIT. Resting place data shown that Anopheles sp. mostly found in piles of clothes and shoe racks. Recorded factors that affect mosquitos bionomics are temperature, humidity, salinity, pH, community behavior, and the presence of livestock. Environmental factors (temperature, humidity, salinity, and pH), habitual behavior of host (3M action, the habit of using insect repellent, mosquito repellent, and mosquito nets), the presence of livestock, and the type of bait blood type affect mosquito activity
Preliminary Selection of Potential Probiotic Lactic Acid Bacteria Isolated from Cocoa Beans (Theobroma cacao) Charis Amarantini; Vinsa Cantya Prakasita; Maria Trivonia Sema
Biosfer: Jurnal Tadris Biologi Vol 12, No 1 (2021): Biosfer: Jurnal Tadris Biologi
Publisher : UNIVERSITAS ISLAM NEGERI RADEN INTAN LAMPUNG

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (170.376 KB) | DOI: 10.24042/biosfer.v12i1.7128

Abstract

This study aims to isolate and identify LABs that spontaneously grow fermented cocoa beans that have the potential as probiotics. The isolation was carried out selectively using an MRS agar medium supplemented with 1% CaCO3. LAB's potential as a probiotic was selected based on the acid resistance test, bile salt resistance test, and antimicrobial activity test. The results showed that three isolates of lactic acid bacteria, namely strains K1B1, K2T2, and K3B4 were tolerant of gastric acid's pH conditions (pH 2.5, pH 3, and pH 4). They also can survive the bile salts (0.3%, 0, 5%, and 1%) and have antibacterial characteristics. The three isolates were identified as Lactobacillus Plantarum species (ID 99.9%).
MP-16 Characterization of Avibacterium paragallinarum Caused Infectious coryza/Snot: Satellite Colony Phenomenon Agnesia Endang Tri hastuti Wahyuni; Charles Rangga Tabbu; Sidna Artanto; Tati Aryani; Vinsa Cantya Prakasita
Hemera Zoa Proceedings of the 20th FAVA & the 15th KIVNAS PDHI 2018
Publisher : Hemera Zoa

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (587.497 KB)

Abstract

Infectious coryza (IC) is an acute upper respiratory disease of poultry that can appear in all ages. Some of clinical signs that are commonly seen in IC are rhinitis, facial swelling or edema, lacrimation, anorexia, and retarded growth in young poultry [1.2.3]. The disease can be found worldwide, especially in tropical countries [4]. Infectious coryza is very important in the chicken farm industry in developed and developing countries, including Indonesia [5]. The large economic losses due to IC such as increased number of culling, decreased egg production (10-40%), decreased body weight, stunting growth, and some mortality (2-10%) [4].Avibacterium paragallinarum which was previously classified as Haemophilus paragallinarum is a causative agent of infectious coryza in laying and broiler chickens, quail, pearl chicken, turkey, and peacocks [4,6,7,8]. The bacteria is commensal in the mucous membrane of the upper respiratory system, is sensitive to preservation and does not last long outside the host body [8]. Factors X and V are needed for the growth of several types of A. paragallinarum. According to in vitro growth requirements, A. paragallinarum can be either nicotinamide adenine dinucleotide (NAD) independent or NAD-dependent. The reduced form of NAD (NADH; 1.56-25 µg/ml) and the oxidized form (20-100 µg/ml) is required for in vitro growth in most isolates A. paragallinarum that show satellitic colony on a medium [9]. The description of the need for V factor of field isolates A. paragalinarum has been few reported. The aim of this research is to find out the phenomenon of satellite colonies from a variety of poultry isolates.
THE EFFECT OF KESAMBI BARK EXTRACT ON MICE LYMPHOCYTE COUNT AND SPLEEN INDEX Christine Stefani Tamelan; Kukuh Madyaningrana; Vinsa Cantya Prakasita
BIOLINK (Jurnal Biologi Lingkungan Industri Kesehatan) Vol 8, No 2 (2022): Februari 2022
Publisher : Universitas Medan Area

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31289/biolink.v8i2.6039

Abstract

Kesambi is a plant widely used by people of Timor Island. Flavonoid content of Kesambi is potential to be used as immunomodulators. This study aimed  to study the effect of Kesambi bark extract on lymphocyte count and spleen index of male mice as an indicator of their immune responses. Research included identification of phytochemical compounds macerated from Kesambi bark, lymphocytes count and spleen index calculation. Mice injected with sheep erythrocytes were treated orally with three doses of Kesambi extract (0.113 mg/g BW, 0.491 mg/g BW, and 0.712 mg/g BW) for 10 days. Lymphocyte count using blood smear method was carried out on day 0, 5 and 10, while lymphocyte count using hematology analyzer and spleen index measurement were performed after mice sacrificed. Kesambi bark contains alkaloids, flavonoids, tannins, and steroids. Treatment with Kesambi bark extract reduced mice lymphocyte count. Administration of 0.491 mg/gr BW dose reduced the mice lymphocytes count close to the number of lymphocytes in the negative control group. The spleen index of mice had no correlation with changes in the number of lymphocytes. This preliminary study shows a potential use of Kesambi bark extract as a natural immunosuppressant.
Potensi ekstrak daun sendok (Plantago major L.) dan serai (Cymbopogon citratus L.) sebagai feet sanitizer alami Relisantika Yulia Sinaga; Aniek Prasetyaningsih; Vinsa Cantya Prakasita
Prosiding Seminar Biologi Vol 6 No 1 (2020): PROSIDING SEMINAR NASIONAL BIOLOGI DI ERA PANDEMI COVID-19 (OKTOBER 2020)
Publisher : Jurusan Biologi, Fakultas Sains dan Teknologi, Universitas Islam Negeri Alauddin Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24252/psb.v6i1.15864

Abstract

Staphylococcus epidermidis merupakan bakteri yang tergolong dalam bakteri Gram positif, berbentuk kokus dan merupakan bakteri yang berada pada kulit manusia. Bakteri ini dapat menyebabkan bau tidak sedap pada kaki. Dalam menghambat pertumbuhan bakteri S. epidermidis digunakan dua tanaman yang mengandung metabolit sekunder seperti alkaloid, flavonoid, steroid, saponin dan terpenoid. Senyawa metabolit sekunder yang terkandung pada tanaman Daun sendok dan serai telah dilaporkan mampu menghambat pertumbuhan bakteri S. epidermidis. Metode ekstraksi kedua tanaman tersebut digunakan maserasi pelarut etanol 96%. Uji fitokimia dengan beberapa uji seperti alkaloid, flavonoid, steroid, terpenoid, dan saponin. Uji disc diffusion pada tanaman secara individu maupun kombinasi, uji sediaan feet sanitizer, uji hedonik dengan membagikan kuisioner dan uji evaluasi produk yaitu uji organoleptik, tekstur, aroma, warna dan uji bakteri produk. Dalam uji zona hambat diperoleh zona hambat sebesar 0,7 cm terdapat pada konsentrasi 40% yang tergolong kuat karena dapat menghambat dan mengurangi pertumbuhan bakteri. Produk feet sanitizer yang dihasilkan memiliki pH 7, memiliki tampilan fisik yang baik dengan SNI 2017, sehingga dapat disimpulkan bahwa produk feet sanitizer yang dihasilkan memiliki kualitas yang baik.
Karakteristik Avibacterium paragallinarum Isolat Lapang : Inokulasi pada Telur Ayam Berembrio umur 7 hari Lynda Nugrahaning Imanjati; Sruti Listra Adrenalin; Ima Fauziah; Vinsa Cantya Prakasita; Sitarina Widyarini; Agnesia Endang Tri Hastuti Wahyuni
Jurnal Sain Veteriner Vol 38, No 3 (2020): Desember
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jsv.46408

Abstract

Infectious coryza (IC) adalah penyakit bakterial yang menyerang saluran pernapasan ayam, yang dapat bersifat akut sampai kronis yang disebabkan Avibacterium paragallinarum. IC merusak saluran pernapasan bagian atas, terutama rongga hidung. Pengamatan perubahan makroskopik pada embrio yang mengalami kematian setelah inokulasi pada telur ayam berembrio (TAB) specific pathogenic free (SPF) sebagai salah satu karakteristik isolat A. paragallinarum belum banyak dilakukan. Penelitian ini bertujuan untuk mengetahui karakteristik isolat A. paragallinarum melalui inokulasi pada telur ayam berembrio TAB SPF umur 7 hari. TAB SPF umur 7 hari berjumlah 50 butir dibagi menjadi 5 kelompok masing-masing terdiri dari 10 butir yaitu kelompok kontrol negatif, A. paragallinarum serotipe A/221; serotipe B/Spross; serotipe B/2448; dan serotipe C/2447. Bakteri terlebih dahulu dikultur pada media cair dan diinkubasi selama 24 jam pada suhu 37oC. Suspensi bakteri dengan volume 0,2 ml (6x108 cfu/ml) diinjeksikan pada TAB SPF, diinkubasi pada suhu 37oC dan diamati adanya kematian sebanyak 2 kali sehari. Embrio yang mati dilakukan skoring perubahan makroskopik. Embrio yang berasal dari TAB SPF pada kelompok yang diinokulasi A. paragallinarum serotipe A/ 221, B/Spross, B/2448, and C/2447 mengalami hemoragi dan kekerdilan. Keseluruhan isolat A. paragallinarum bersifat patogen pada telur ayam berembrio.
Virulence Characteristic of Avian Pathogenic Escherichia coli (APEC) Isolates Sruti Listra Adrenalin; Lynda Nugrahaning Imanjati; Ima Fauziah; Vinsa Cantya Prakasita; Sitarina Widyarini; Agnesia Endang Trihastuti Wahyuni
Jurnal Sain Veteriner Vol 38, No 1 (2020): April
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jsv.46494

Abstract

Avian pathogenic Escherichia coli (APEC) is a cause of colibacillosis in poultry, one of the respiratory disease that causes serious problems in the poultry industry. The APEC can cause high mortality and culling, decreased production, and high costs of treatment. Manifestations of colibacillosis are airsacculitis, perihepatitis, and pericarditis. The APEC serotypes that are widely identified in the field are O1K1, O2K1, and O78K80. Embryo lethality assay (ELA) is a method for determine the virulence of APEC serotypes. The aim of this study was to determine the virulence characteristic of APEC isolates. Five APEC serotypes O1K1, O2K1, O78K80, O157H7, and unknown serotype were used for ELA method by inoculated E. coli into chorioallantoic of specific pathogen free 12-days old embryos. Each group of 10 embryos, inoculated E. coli dose of 100-500 CFU/ 0,1 ml. Candling was carried out for 6 days (18-days old embryo) to determined the mortality and pathological lesions. The percentage of embryo mortality post-inoculated with APEC O1K1, O2K1, unknown serotypes were 100% (10/10), O78K80 serotype was 90% (9/10), and O157H7 serotype was 70% (70%). Lesions of all embryos were cranial and extremity hemorrhage. In this study, E. coli isolates had high virulence. 
Peluang Imbuhan Pakan Herbal-Probiotik Komersial “Promix®” Sebagai Pengganti Antibiotic Growth Promoter (AGP) pada Ayam Pedaging yang Diberi Vaksin ND Agnesia Endang Tri H. Wahyuni; Vinsa Cantya Prakasita; Thomas Emanuel Manggotu Nahak; Agustina Viktoria Tae; Jeffi Chandra; Ajiguna Ajiguna; Sruti Listra Adrenalin; Lynda Nugrahaning Imanjati; Ima Fauziah
Jurnal Sain Veteriner Vol 37, No 2 (2019): Desember
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (301.894 KB) | DOI: 10.22146/jsv.48614

Abstract

With the prohibition of the use of Antibiotic growth promotors (AGP) used in animal feed, especially in broilers, the use of feed additives such as herbs, probiotics, prebiotics, synbiotics or mixtures of some of these ingredients is currently widely used as feed replacements for AGP substitutes. One of the commercial feed additives that contain combinations herbal and probiotics is Promix®. In broiler maintenance, one vaccine that must be given is Newcastle Disease (ND) vaccine. The purpose of this study was to determine the role of supplementation of commercial feed combination of herbs and probiotics in broilers given ND vaccines. A total of 42 broilers were divided into two, group A (vaccinated with ND) and B (not vaccinated ND), each with 21 heads. Group A is divided into groups A1, A2 and A3 each with 7 heads. The A1 group is given only basal feed; A2 basal feed and AGP; A3 basal feed and Promix®. Group B is divided by B1, B2 and B3 same as group A before. Broilers are maintained for 5 weeks and weighing each week. All data obtained were statistically analyzed using One Way ANOVA and continued with Duncan's post hoc test. The results of this study were that the group of chickens given the ND vaccine and given supplements of Promix® had greater weight gain (P˂0.05) compared to the AGP and basal groups in the fifth week while in the group of chickens that were not given the ND vaccine but given supplement Promix® feed had greater weight gain (P˂0.05) compared to the AGP and basal groups in the fourth week. In the fifth week there was no significant difference (P˂0.05) between all feed groups in the group not given the ND vaccine. The conclusion of this study that feed additives contain combinations herbal and probiotics can replace AGP as feed additive in broiler. By giving ND vaccine the weight gain of the feed group plus Promix® showed a significant increase in weight gain.
UJI EFEKTIVITAS EPIKARPIUM BUAH NANGKA (artocarpus heterophyllus lamarck.) SEBAGAI SEDIAAN KRIM TABIR SURYA UV-B Tara Inastu Kandarp; Aniek Prasetyaningsih; Vinsa Cantya Prakasita
EduMatSains : Jurnal Pendidikan, Matematika dan Sains Vol 6 No 1 (2021): Juli
Publisher : Fakultas Keguruan dan Ilmu Pendidikan, Universitas Kristen Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33541/edumatsains.v6i1.2798

Abstract

Sunscreen is needed as protection against the skin from exposure to UV rays, because it will cause erythema, aging skin, blistering, and skin cancer, in skin that is not protected by sunscreen. This study aims to determine the in vitro and in vivo ability of epicarpium A. heterophyllus extract sunscreen cream preparations. This research was conducted in vitro and in vivo, beginning with the extraction process with the maceration method using 70% ethanol, identification of compounds in the extract, TLC, GC-MS, total flavonoids test, physical quality of cream preparations, SPF values ​​of extracts and creams, and in vivo sunscreen activity tests were conducted. The yield percentage produced from 450 grams of simplicia powder was 47.61%. The compounds in A. heterophyllus epicarpium extract are flavonoids, tannins, saponins, terpenoids, steroids, fatty acid groups, alcohol groups, and alkanes. Crude extract contains total flavonoids of 43.625 mgQE / g extract. The 75% concentration in the cream preparation had the highest SPF value of 8.173. The in vivo test used the MED (Minimal Erythema Dose) method of erythema scoring. A concentration of 75% gives the lowest MED value of 0.25. This research is expected to provide information on alternative uses of natural ingredients as the main ingredient of sunscreen so that the use of chemicals as the main ingredient will be reduced.
Co-Authors Adrenalin, Sruti Listra Agnesia Endang Tri H. Wahyuni Agnesia Endang Tri Hastuti Wahyuni Agustina Dwi Wijayanti Agustina Viktoria Tae Ajiguna Ajiguna Anak Agung Gede Sugianthara Angelia Astria Aniek Prasetyaningsih Aniek Prasetyaningsih Aniek Prasetyaningsih Anjela Noya CHARIS AMARANTINI Charles Rangga Tabbu Christine Stefani Tamelan Djoko Rahardjo Djoko Rahardjo Dwi Aditiyarini Fauziah, Ima Feblicya Palimbunga Ima Fauziah Ima Fauziah Ima Fauziah Imanjati, Lynda Nugrahaning Irein Kusuma Angreini Jade Septhimoranie Jeffi Chandra Kukuh Madyaningrana Lynda Nugrahaning Imanjati Lynda Nugrahaning Imanjati Lynda Nugrahaning Imanjati Maria Trivonia Sema Marlin Cindy Claudya Malelak Pepiana, Marlen Aviati Sarah Priskila Melinia Wuni Relisantika Yulia Sinaga Setya Harianto Sidna Artanto Sitarina Widyarini Sitarina Widyarini Sruti Listra Adrenalin Sruti Listra Adrenalin Sruti Listra Adrenalin Tara Inastu Kandarp Tati Aryani Thomas Emanuel Manggotu Nahak