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Journal : Edumatsains

POTENSI BIOAKARISIDA EKSTRAK DAUN MIMBA (Azadirachta Indica) DAN BUNGA CENGKEH (Syzygium Aromaticum) TERHADAP TUNGAU PENYEBAB PENYAKIT KREPES PADA JAMUR KUPING Meilani Apra; Aniek Prasetyaningsih; Kukuh Madyaningrana
EduMatSains : Jurnal Pendidikan, Matematika dan Sains Vol 5 No 2 (2021): Januari
Publisher : Fakultas Keguruan dan Ilmu Pendidikan, Universitas Kristen Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33541/edumatsains.v5i2.2241

Abstract

Wood ear mushrooms is commonly known as one of the cultivated edible mushrooms in Indonesia. The demand for this commodity is still steadily high and leads mushroom farmers to optimize its cultivation. For mushroom farmers in Yogyakarta, the prevalence of krepes disease which is caused by a group of species belongs to Acarina order is still high and leads to total loss of harvest. In order to diminish these Acarina species, the use of chemical acaricides is common because of their effectiveness in eradicating the pests despite their toxic effect to the environment. A strategy to provide environmental friendly acaricides which are extracted from plants is therefore important. This research aimed to study the potency of neem leaves (Azadirachta indica) and clove flowers (Eugenia caryophyllata) extracts as bioacarisides for Acarina species as the causal agent of krepes disease. Neem leaves and cloves flowers were extracted using maceration and soxhlet extraction methods, using 96% methanol as their solvents. Obtained crude extracts would then be subjected to phytochemical analysis using qualitative biochemical tests and GC-MS. The effectivity of extracts as bioacaricides were tested both in in vitro and field scale. In vitro tests showed that neem leaves extract and clove flower extract had LC50 value ​​of 0.22% and 0.48% respectively. Field scale test on mushroom baglogs showed that the efficacy of the neem leaves and clove flower to eradicate acarina were above 50%. Taken together, neem leaves and clove flower extracts are potential to be used as bioacaricides in mushroom farming.
POTENSI EKSTRAK DAUN BINAHONG (Anredera cordifolia (Ten.) Steenis) DAN MINYAK ATSIRI SERAI (Cymbopogon citratus (DC.) Stapf) SEBAGAI BAHAN AKTIF HAND SANITIZER SPRAY Adonia Felma Mosse; Aniek Prasetyaningsih; Dwi Adityarini
EduMatSains : Jurnal Pendidikan, Matematika dan Sains Vol 6 No 1 (2021): Juli
Publisher : Fakultas Keguruan dan Ilmu Pendidikan, Universitas Kristen Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33541/edumatsains.v6i1.2474

Abstract

Maintaining hand hygiene is an important habit to prevent disease caused by microorganisms and viruses. Handsanitizer is one of hygiene products as antibacterial and practical use. However, the use of alcohol as its main ingredient could irritate skin. Therefore, the alternative of alcohol is urgently found to prevent this side effect in same effectiveness. in this research, Binahong (Anredera cordifolia (Ten.) Steenis leaf extract and lemongrass oil (Cymbopogon citratus (DC.) Stapf) are used to replace alcohol as the main ingredient. This study aims to know the potential antibacterial activity of binahong leaf extract, lemongrass oil and its combination against S.aureus and its effectiveness as an active ingredient in hand sanitizer spray. Binahong extraction using the maceration method. The secondary metabolites of both extracts were identified by phytochemical assay. Then, their antibacterial activity was determined using a disk diffusion test for S. aureus. The organoleptic of hand sanitizer spray was observed. The highest antibacterial activity was obtained from the combination of binahong leaf extract and lemongrass oil 100% at ratio 1:1. This combination was used to formulate the hand sanitizer spray. The highest inhibitory activity of hand sanitizer spray was shown in the formulation with 3% active ingredients (extracts). This product had dark yellow in colour, distinctive aroma, and liquid texture and effectively suppresses bacterial growth on the hands. According to the results, binahong leaf extract and lemongrass oil are potentially used as active ingredients for natural hand sanitizer sprays.
UJI EFEKTIVITAS EPIKARPIUM BUAH NANGKA (artocarpus heterophyllus lamarck.) SEBAGAI SEDIAAN KRIM TABIR SURYA UV-B Tara Inastu Kandarp; Aniek Prasetyaningsih; Vinsa Cantya Prakasita
EduMatSains : Jurnal Pendidikan, Matematika dan Sains Vol 6 No 1 (2021): Juli
Publisher : Fakultas Keguruan dan Ilmu Pendidikan, Universitas Kristen Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33541/edumatsains.v6i1.2798

Abstract

Sunscreen is needed as protection against the skin from exposure to UV rays, because it will cause erythema, aging skin, blistering, and skin cancer, in skin that is not protected by sunscreen. This study aims to determine the in vitro and in vivo ability of epicarpium A. heterophyllus extract sunscreen cream preparations. This research was conducted in vitro and in vivo, beginning with the extraction process with the maceration method using 70% ethanol, identification of compounds in the extract, TLC, GC-MS, total flavonoids test, physical quality of cream preparations, SPF values ​​of extracts and creams, and in vivo sunscreen activity tests were conducted. The yield percentage produced from 450 grams of simplicia powder was 47.61%. The compounds in A. heterophyllus epicarpium extract are flavonoids, tannins, saponins, terpenoids, steroids, fatty acid groups, alcohol groups, and alkanes. Crude extract contains total flavonoids of 43.625 mgQE / g extract. The 75% concentration in the cream preparation had the highest SPF value of 8.173. The in vivo test used the MED (Minimal Erythema Dose) method of erythema scoring. A concentration of 75% gives the lowest MED value of 0.25. This research is expected to provide information on alternative uses of natural ingredients as the main ingredient of sunscreen so that the use of chemicals as the main ingredient will be reduced.
UJI EFEKTIVITAS SALEP KULIT BATANG KAPUK RANDU (Ceiba Pentandra) SEBAGAI OBAT ANTI-INFLAMASI Setya Harianto; Aniek Prasetyaningsih; Vinsa Cantya Prakasita
EduMatSains : Jurnal Pendidikan, Matematika dan Sains Vol 6 No 1 (2021): Juli
Publisher : Fakultas Keguruan dan Ilmu Pendidikan, Universitas Kristen Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33541/edumatsains.v6i1.2799

Abstract

Inflammation is protective response of vascular tissue to various harmful stimuli that characterized by pain, redness, and swelling. Empirically, stem bark of Ceiba pentandra has various chemical compounds that nutritious for health and have potential to be used as anti-inflammatory. This research will provide information the benefit of C. pentandra stem bark especially as anti-inflammatory agents and it’s correlation with the compounds. Maceration method with 70% ethanol was used in extraction and the yield was 75,54%. In phytochemical test, it was identified in crude extract contained flavonoids, tannins, saponins, steroids, β-sitosterol, 1,2-benzendiol, quinic acid, and octadec-9-enoic acid. On extract and ointment products C. pentandra has antioxidants activity with IC50 value of extract was 52,64 ppm and IC50 value of ointment products was 117,64 ppm. This research is included RAL experimental which was carried out on male mice that divided into 5 groups: negative control (ointment base), positive control (Betametasone valerate 0,1%), and 50%, 75%, 100% of C. pentandra ointment. The ointment was applied after the mice were induced by carrageenan, measured with the parameter of skinfold thickness of mice’s back every hour for 6 hours of observation. The result showed C. pentandra stem bark ointments has anti-inflammatory effect which %PI value in the 50%, 75%, 100% were respectively 10,85%; 18,99%; 30,84%.