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All Journal AGRISAINS AL KAUNIYAH Proceedings of KNASTIK Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati JURNAL PEMULIAAN TANAMAN HUTAN Biology, Medicine, & Natural Product Chemistry Pro-Life Edumatsains Jurnal Inovasi Hasil Pengabdian Masyarakat (JIPEMAS) Jurnal Pembelajaran dan Biologi Nukleus Sciscitatio : Journal for Biological Science Jurnal Agrohut
Aniek Prasetyaningsih
Program Studi Biologi, Fakultas Bioteknologi, Universitas Kristen Duta Wacana
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Education Engineering Environmental Science Immunology & microbiology Languange, Linguistic, Communication & Media Materials Science & Nanotechnology Mathematics Medicine & Pharmacology Physics Public Health Other

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Journal : Jurnal Pembelajaran dan Biologi Nukleus

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Browning Prevention Method in Kepel (Stelechocarpus burahol (Blume) Hook.f. & Thomson) in Vitro Culture Devi Sonti Sibarani; Ratih Restiani; Aniek Prasetyaningsih
JURNAL PEMBELAJARAN DAN BIOLOGI NUKLEUS (JPBN) Vol 10, No 1 (2024): Jurnal Pembelajaran Dan Biologi Nukleus March 2024
Publisher : Fakultas Keguruan dan Ilmu Pendidikan Universitas Labuhanbatu

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36987/jpbn.v10i1.5464

Abstract

Browning of explants is a common problem in Kepel (Stelechocarpus burahol (Blume) Hook.f. & Thomson) in vitro culture, resulting in low explant in vitro regeneration of Kepel. Using nodal explant, the effect of explant immersion in ascorbic acid, the addition of ascorbic acid to the media, and dark and light incubation conditions were investigated. The browning prevention method was selected based on the delayed browning appearance time, the lowest broning intensity, and the highest percentage of callus. This present study used a completely randomized design with treatment variations: immersion explant in ascorbic acid 100 mg/L, addition of ascorbic acid 100 and 200 mg/L to the media, addition of ascorbic acid 100 and 200 mg/L and activated charcoal 1 g/L to the media, and incubation in dark and light conditions for 28 days. The results showed that the combination of the addition of ascorbic acid 200 mg/L to MS media and incubation in dark conditions were effective browning prevention methods in inhibiting browning appearance time (5 DAP), significantly reducing browning intensity (0.3), and increasing callus growth (100%) of kepel node explants during 28 days of culture. The results of this study are useful in establishing protocols for in vitro culture of Kepel plants, especially at the initiation stage, and can be expected to support the successful conservation of Kepel plants through in vitro propagation. Keywords: Ascorbic acid, Activated Charcoal, Browning, In Vitro Culture, Stelechocarpus burahol
Co-Authors Adonia Felma Mosse Angelia Astria AYPBC Widyatmoko Catarina Aprilia Ariestanti Devi Sonti Sibarani Djima, Elza Gitaa Eirene Djima Djoko Rahardjo Djoko Rahardjo Dwi Aditiyarini Dwi Adityarini Elisabet Suharti Soleman Elsih Dian Ariningsih Kania Apenta Olisvelos Kisworo Kisworo Kukuh Madyaningrana Lavenia, Jovita Maria Grasela Kase Meilani Apra Nurlia Widayanti Ratih Restiani Santoso, Julyana Dika Seta Nurhayati Mularum Setya Harianto Tara Inastu Kandarp Tiffany, Yollanda Vinsa Cantya Prakasita Willy Sudiarto Raharjo Wulan Krista Waty