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All Journal HAYATI Journal of Biosciences BERITA BIOLOGI JURNAL BIOLOGI INDONESIA Makara Journal of Science
Tri Puji Priyatno
Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumber Daya Genetik Pertanian, Jl. Tentara Pelajar 3A, Bogor 16111 Telp. (0251) 8337975
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Earth & Planetary Sciences

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Analisis Molekuler Piramida Gen Xa pada Progeni Padi Varietas Ciherang dan Inpari 13 ., Fatimah; Prasetiyono, Joko; Priyatno, Tri Puji; Yunus, Muhammad; Suhartini, Tintin; Ridwan, Iman; Baroya, Mushlihatun
JURNAL BIOLOGI INDONESIA Vol 11, No 1 (2015): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v11i1.2161

Abstract

Bacterial leaf blight (BLB) is a major disease in Indonesian lowland rice.This research was undertaken to pyramid three BLB resistant genes xa5, Xa7 and Xa21 and one background BLB resistant gene Xa4 into Ciherang and Inpari 13 varieties. The donor parent Code (Xa4+Xa7) was crossed with Angke (Xa4+xa5) while Ciherang and Inpari 13 were crossed with IRBB21 (Xa21). Progenies were selected using marker assisted selection and yield component observation. Foreground selection was conducted using SSR and STS markers linked with the targeted genes in the F1 and DCF1 population. Individuals with triple positives Xa genes were screened for the presence of Xa4 gene as the background. Selected heterozygote plants in F1 Code x Angke, F1 Ciherang x IRBB21 and F1 Inpari 13 x IRBB21 were used to develop DCF1 population. Molecular analysis on DCF1 population through alleles of three BLB resistant genes xa5, Xa7 and Xa21 and one background BLB resistant gene Xa4 resulted 8 (2,6%) in DCF1 Ciherang and 13 (3,5%) in DCF1 Inpari 13. Yield component characters on F1 Code x Angke resulted significant in number of panicle. F1 Ciherang x IRBB21, F1 Inpari 13 x IRBB21 and DCF1 Ciherang resulted significant in weight of empty grain while DCF1 Inpari 13 resulted no significance in all of observed characters. Keywords: Rice, F1 Population, DCF1 Population, molecular marker, Xa gene 
EKSPLORASI DAN KARAKTERISASI ENTOMOPATOGEN ASAL BERBAGAI INANG DAN LOKASI [Exploration and Characterization of Entomopathogenic from Various Host and Location] Priyatno, Tri Puji; Samudra, I Made; Manzila, Ifa; Susilowati, Dwi Ningsih; Suryadi, Yadi
BERITA BIOLOGI Vol 15, No 1 (2016)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (3631.714 KB) | DOI: 10.14203/beritabiologi.v15i1.2859

Abstract

Microbial groups of entomopathogenic (fungi and bacteria) had been reported causing insect mortality. The aim of the study was to explore and characterized entomopathogenic from various host and locations. Fungal identification at genus and species level was caried out based on conidial morphology, hyphal growth, conidiophore and colony color; whilst for bacterial identification was based on standard Bergey’s manual for determinative bacteria. Sixteen entomopathogenic isolates that consisted of fungal and bacteria have been collected and preserved for further characterization. Of the 16 entomopathogen collected samples, five fungal genera was found i.e. Paecilomyces; Metarhizium, Beauveria, Hirsutella; and Cordyceps. Seven isolates belonging to six fungal isolates, and one bacterial isolate had been identified based upon ITS and 16S rDNA sequences, respectively. We confirmed that 6 fungal isolates belong to species of Paecilomyces reniformis, B. bassiana, M. anisopliae, M. anisopliae var acridum, Hirsutella thomsonii. One isolate of red pigmented bacteria Sm201102 have been identified was belonging to species Seratia marcescence. It was also obtained two fungal isolates from different host (spider and beetle) which confirmed by morphological character belong to Cordyceps sp.
KARAKTERISASI BAKTERI PENGHASIL ASAM INDOL ASETAT DAN PENGARUHNYA TERHADAP VIGOR BENIH PADI Lestari, Puji; Suryadi, Yadi; Susilowati, Dwi Ningsih; Priyatno, Tri Puji; Samudra, I Made
BERITA BIOLOGI Vol 14, No 1 (2015)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v14i1.1859

Abstract

The ability to produce indole acetic acid (IAA) by endophytic bacteria is one of the basic criteria for the use of bacteria as plant growth promoter agent which is essential for the agricultural production.The objectives of this study were to evaluate the ability of 17 bacterial isolates to produce IAA and its effect on improvement of rice seed germination and molecular identification of the selected isolates based on the 16S rRNA gene. The IAA content was determined using Salkowski method measured by spectrophotometer UV-Vis and the effect of endophytic bacteria inoculation on seed germination was done by in vitro assay. Sequences of the selected isolates 16S rRNA amplified by PCR were analyzed the homology against bacterial 16S rRNA database in Genebank. IAA values ranged from 6.632 to 50.053 mg/L with the highest IAA production shown by isolate 6KJ which was followed by 4PB (41.807 mg/L). Bacterial IAA increased rice seed vigor significantly compared to control. However, bacterial inoculation with different concentrations of IAA did not significantly affect the growth of rice plants. Based on the IAA and its effect on seed vigor, 6KJ, 4PB and 2KB were selected for molecular identification. Results showed that the three isolates belonged to Bacillus genus, 6KJ as B. aryabhattai, 4PB belonging to B. cibi and 2KB having 97% homology with B. marisflavi. Further evaluation of the selected endophytic isolates producing IAA is necessary to be carried out to explore their potency as a source of hormone to promote plant growth.
KARAKTERISASI ß-1,3-1,4-GLUKANASEBAKTERI ENDOFITIK BURKHOLDERIA CEPACIA ISOLATE76 ASAL TANAMAN PADI Manzila, Ifa; Priyatno, Tri Puji; Fathin, Muhammad Faris; Ambarsari, Laksmi; Suryadi, Yadi; Samudera, I Made; Susilowati, Dwi Ningsih
BERITA BIOLOGI Vol 14, No 2 (2015)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v14i2.1819

Abstract

Pathogenic fungus is one of the constraints to increase crop production. Chemical control using fungicides caused negative effects either to the environment or increased pathogen resistance to fungicide. Biological control using microbial-producing ß glucanase is an alternative method to inhibit the growth of pathogenic fungus. The aim of this study was to characterize ß-1,3-1,4-glucanase produced by rice endophytic bacterium, B. cepacia E76. Purification was carried out by ammonium sulphate precipitation, dialysis, and ion exchange chromatography using DEAE sepharose Fast Flow. A further characteristic of the enzyme activity was studied using oatmeal-glucan substrate.Results showed that precipitation using saturated 80% ammonium sulphate generated a good yield with the purity increased by 11 fold and yield of 66%.After chromatography step, the ß-1,3-1,4-glucanase of B. cepacia was successfully purified with an increasedof purity up to 33 fold and yield of 4%. Based on 10% SDS-PAGE, the enzyme profiles had the molecular weight of 15, 48 and 55 kDa.Of the three isozymes, only the 48 kDa isozyme showed the strongest glucanase activity when grown on media containing glucan as substrate.
ANALISIS MOLEKULER PIRAMIDA GEN XA PADA PROGENI PADI VARIETAS CIHERANG DAN INPARI 13 ., Fatimah; Prasetiyono, Joko; Priyatno, Tri Puji; Yunus, Muhammad; Suhartini, Tintin; Ridwan, Iman; Baroya, Mushlihatun
JURNAL BIOLOGI INDONESIA Vol 11, No 1 (2015): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v11i1.2161

Abstract

Bacterial leaf blight (BLB) is a major disease in Indonesian lowland rice.This research was undertaken to pyramid three BLB resistant genes xa5, Xa7 and Xa21 and one background BLB resistant gene Xa4 into Ciherang and Inpari 13 varieties. The donor parent Code (Xa4+Xa7) was crossed with Angke (Xa4+xa5) while Ciherang and Inpari 13 were crossed with IRBB21 (Xa21). Progenies were selected using marker assisted selection and yield component observation. Foreground selection was conducted using SSR and STS markers linked with the targeted genes in the F1 and DCF1 population. Individuals with triple positives Xa genes were screened for the presence of Xa4 gene as the background. Selected heterozygote plants in F1 Code x Angke, F1 Ciherang x IRBB21 and F1 Inpari 13 x IRBB21 were used to develop DCF1 population. Molecular analysis on DCF1 population through alleles of three BLB resistant genes xa5, Xa7 and Xa21 and one background BLB resistant gene Xa4 resulted 8 (2,6%) in DCF1 Ciherang and 13 (3,5%) in DCF1 Inpari 13. Yield component characters on F1 Code x Angke resulted significant in number of panicle. F1 Ciherang x IRBB21, F1 Inpari 13 x IRBB21 and DCF1 Ciherang resulted significant in weight of empty grain while DCF1 Inpari 13 resulted no significance in all of observed characters. Keywords: Rice, F1 Population, DCF1 Population, molecular marker, Xa gene 
Functional Analysis of an Appressorium-Specific Gene from Colletotrichum gloeosporioides Priyatno, Tri Puji; Bakar, Farah Diba Abu; Redzuan, Rohaiza Ahmad; Mahadi, Nor Muhammad; Murad, Abdul Munir Abdul
HAYATI Journal of Biosciences Vol. 27 No. 2 (2020): April 2020
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (651.352 KB) | DOI: 10.4308/hjb.27.2.107

Abstract

A novel gene (CAS2) specifically expressed during appressorium formation was isolated from Colletotrichum gloeosporioides using Differential Display RT-PCR. CAS2 comprises 368 deduced amino acid residues and is 50% identical to a hypothetical protein from Chaetomium globosum. ProtFun 2.2 server analysis predicted that Cas2 functions as a transport and binding protein. Based on putative transmembrane domain prediction software (HMMTOP), Cas2 protein is composed of five alpha-helical transmembrane domains with a very short external N-terminus tail and long internal C-terminus. ExPASy ScanProsite analysis showed the presence of integrin beta chain cysteine-rich domain, N-myristoylation site, EGF-like domain, 2Fe-2S ferredoxins, iron-sulfur binding region, VWFC domain, fungal hydrophobins signature, membrane lipoprotein lipid attachment site, and Janus-faced atracotoxin (J-ACTX) family signature in CAS2 protein. Mutants with deleted CAS2 were not significantly different in terms of vegetative growth, conidiation, and appressoria production compared to wild type. However, the Cas2 mutant produced multipolar germination, a feature which distinguishes it from wild type strain. Interestingly, the mutant is non-virulent to mango fruits, indicating that CAS2 may encode proteins that function as novel virulence factors in fungal pathogens.
The Use of Molecular Markers to Analyze the Genetic Diversity of Indonesian Pepper (Capsicum spp.) Varieties Based on Anthracnose Resistance Nugroho, Kristianto; Terryana, Rerenstradika T.; Manzila, Ifa; Priyatno, Tri Puji; Lestari, Puji
Makara Journal of Science
Publisher : UI Scholars Hub

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Anthracnose is an important disease affecting the pepper plant and can lead to significant decreases in harvest yield. In this study, the genetic diversity of Indonesian pepper varieties was analyzed based on anthracnose resistance using molecular markers. DNA collected from 15 pepper varieties belonging to two species—Capsicum annuum L. and C. frutescens L.—was amplified using 14 molecular markers. The fungal isolate Colletotrichum capsici was inoculated into ripe harvested pepper fruits to observe their resistance to anthracnose as indicated by lesion size. Phylogenetic analysis revealed that the 15 pepper varieties could be classified into two major clusters with a genetic similarity coefficient of 0.63, and the pepper varieties exhibited varying degrees of resistance to anthracnose based on lesion size. Using the molecular markers, we were able to differentiate the species of pepper varieties, but not their resistance to anthracnose. All markers used in this study were confirmed to be highly informative (PIC > 0.5), suggesting their potential use in genetic studies on peppers. The marker GPMS29 was found to be significantly associated (P < 0.05) with anthracnose resistance. This information about the genetic diversity of peppers—along with the molecular markers used in our study—could prove to be useful in the further development of breeding programs of pepper plants in terms of anthracnose resistance in Indonesia.
Determination of Pathotypes from Indonesian Xanthomonas oryzae Pv. Oryzae Population causing Bacterial Leaf Blight and their Reactions on Differential Rice Suryadi, Yadi; Samudra, I Made; Priyatno, Tri Puji; Susilowati, Dwi Ningsih; Lestari, Puji; Fatimah,; Kadir, Trini Suryani
Makara Journal of Science Vol. 20, No. 3
Publisher : UI Scholars Hub

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Abstract

The objective of this work was to study the virulence of 15 Xanthomonas oryzae pv. oryzae (Xoo) isolates collected in three provinces in Indonesia (North Sumatra, South Sumatra, and South Sulawesi) based on five Indonesian differential rice genotypes and 10 near isogenic lines (NILs) that have been known to differ in their resistance to bacterial leaf blight (BLB), in a greenhouse assessment. In addition, this study also aims to monitor the responses of 31 rice genotypes (21 NILs, five differentials, five improved cultivars) to BLB disease in a field experiment. The 15 isolates showed different virulence patterns on the NILs’genotypes with a single resistance gene indicating the pathogen diversity. Eight different pathotypes were present, as demonstrated by a particular virulence pattern of each isolate on the genotypes. Determination of Xoo pathotype revealed that Xoo pathotypes responded differently based on their reaction to NILs and Indonesian differential genotypes. The field assessment demonstrated the incidence and severity of BLB disease on rice genotypes ranging from 25% to 100% and 5.5% to 72.91%, respectively, while the mean disease index ranged from 1.15% to 72.9%. The disease response varied among rice genotypes. IRBB50 (Xa4+xa5), IRBB51 (Xa4+xa13), IRBB52 (Xa4+Xa21), IRBB53 (Xa4+Xa21), IRBB56 (Xa4+xa5+xa13), IRBB57 (Xa4+xa5+Xa21), IRBB59 (Xa4+xa13+Xa21), IRBB64 (Xa4+xa5+Xa7+Xa21), IRBB66 (Xa4+xa5+Xa7+xa13+Xa21), IRBB7(Xa7), Angke (Xa4+xa5) and Code (Xa4+Xa7) were revealed to be highly resistant to the BLB pathogen. These genotypes have potential as genetic material for the pyramiding of several resistance genes for the development of rice resistance to BLB disease in Indonesia.
Co-Authors Abdul Munir Abdul Murad Bakar, Farah Diba Abu Baroya, Mushlihatun Baroya, Mushlihatun DWI NINGSIH SUSILOWATI Fathin, Muhammad Faris Fatimah Fatimah . I Made Samudera, I Made I Made Samudra Ifa Manzila Iman Ridwan, Iman Joko Prasetiyono KRISTIANTO NUGROHO, KRISTIANTO LAKSMI AMBARSARI Mahadi, Nor Muhammad Muhammad Yunus PUJI LESTARI Redzuan, Rohaiza Ahmad RERENSTRADIKA T. TERRYANA, RERENSTRADIKA T. Tintin Suhartini Trini Suryani Kadir Yadi Suryadi