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Journal : BioEksakta : Jurnal Ilmiah Biologi Unsoed

Hubungan beberapa Aspek Biologi Reproduksi dengan Panjang Tubuh Ikan Palung (Hampala macrolepidota Kuhl & Van Hasselt 1823) di Waduk Panglima Besar Jenderal Soedirman Dhita Rasyida Soraya; Priyo Susatyo; Siti Rukayah
BioEksakta : Jurnal Ilmiah Biologi Unsoed Vol 2 No 3 (2020): BioEksakta
Publisher : Fakultas Biologi Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.bioe.2020.2.3.3481

Abstract

Penelitian ini bertujuan untuk mengetahui mengetahui rasio kelamin, TKG, IKG, fekunditas, ukuran pertama kali matang gonad ikan palung (H. macrolepidota) yang tertangkap di Waduk Panglima Besar Jenderal Soedirman dan hubungan antara beberapa aspek biologi ikan palung dengan panjang tubuh. Pengambilan sampel ikan dilakukan dua kali pada November dan Desember 2017. Hasil penelitian menujukkan bahwa ikan palung di Waduk Panglima Besar Jenderal Soedirman memiliki rasio kelamin 1 : 2,82. Tingkat Kamtangan Gonad (TKG) ikan palung yang ditangkap berada pada TKG I-IV. Rata-rata ukuran pertama kali matang gonad pada ikan palung betina adalah 447,6 mm dan ikan palung jantan 295,8 mm. Fekunditas ikan palung sebanyak 841-35.896 butir. Hubungan TKG dengan panjang total pada ikan betina berkorelasi positif (r = 0,827) dan jantan memiliki korelasi positif (r = 0,566). Hubungan IKG dengan panjang total ikan betina berkolerasi positif (r = 0,670). Hubungan IKG dengan panjang total ikan jantan berkolerasi positif (r = 0,392). Hubungan fekunditas dan panjang total memiliki korelasi positif (r = 0,978)
Status Reproduksi Belut Sawah (Monopterus albus) yang tertangkap di Desa Kalitinggar, Kabupaten Purbalingga Sa'adatul Amalia; Priyo Susatyo; Sugiharto Sugiharto
BioEksakta : Jurnal Ilmiah Biologi Unsoed Vol 2 No 3 (2020): BioEksakta
Publisher : Fakultas Biologi Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.bioe.2020.2.3.3393

Abstract

Ricefield eel is one type of fish whose habitat is in paddy fields and the eel's body does not have scales. Ricefield eel has a high protein, so it is widely consumed and sold in local and international markets. Eel reproduction is a protogynous hermaphrodite thathas a replacement cycle from female sex to the male sex. This research was conducted in the laboratory of the Faculty of Biology, Jenderal Soedirman University, by taking samples of Ricefield Eel in Kalitinggar Village, Padamara District, Purbalingga Regency. The purpose of this study was to know the relationship between GSI with a certain eel body length, as well as the gonad histology picture of rice eels in each fishing month. The results of the study as many as 54 eel catches. Ricefield eel was caught in May, June, and August 2019. Eel body length ranges from 16-35 cm with a GSI value of 0.32 – 10.36 %. There is a correlation between eels' body length toward GSI with the value p < 0.01, which was tested using Pearson correlation in the SPSS application. Based on observations using a microscope, many of the eels produced females with a body length ranges of 19-32 cm.
Diferensial Leukosit Beberapa Spesies Ikan Tangkapan Dari Familia Cyprinidae Di Sungai Banjaran, Kabupaten Banyumas Nabilla Gina Irwanti; Priyo Susatyo; Eko Setio Wibowo
BioEksakta : Jurnal Ilmiah Biologi Unsoed Vol 4 No 3 (2022): BioEksakta
Publisher : Fakultas Biologi Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.bioe.2022.4.3.4703

Abstract

Familia Cyprinidae dapat ditemukan di hampir seluruh sungai Indonesia termasuk Sungai Banjaran, Kabupaten Banyumas. Diferensial leukosit bertujuan mengetahui perbedaan persentase sel leukosit. Penelitian ini untuk mengetahui hubungan antara lokasi pengambilan sampel (hulu, tengah, dan hilir) dengan diferensial leukosit dan mengetahui gambaran apusan darah dari beberapa spesies ikan familia Cyprinidae. Pengambilan sampel menggunakan teknik purposive sampling pada tiga stasiun yang berbeda. Data yang diperoleh dianalisis dengan metode Analysis of Variance (ANOVA) dengan tingkat kesalahan 5% dan gambaran hasil pengamatan mikroskopis jenis-jenis leukosit. Hasil penelitian menunjukkan bahwa perbandingan persentase sel lekosit pada Ikan Nilem (Osteochilus vittatus), Ikan Brek (Barbonymus balleroides), dan Ikan Lunjar (Rasbora argyrotaenia) dengan lokasi Hulu, Tengah, Hilir Sungai Banjaran, Banyumas, berbeda secara signifikan (p<0,05). Persentase sel leukosit yang paling tinggi ada pada zona Hilir di Sungai Banjaran, Banyumas yaitu sel neutrofil dan sel monosit. Presentase sel neutrofil Ikan Nilem didapatkan sebesar 9,5%, Ikan Brek sebesar 8,5% dan Ikan Lunjar sebesar 9,4%. Presentase sel monosit pada Ikan Nilem sebesar 8,9%, Ikan Brek sebesar 8,7% dan Ikan Lunjar sebesar 8,5%. Sel neutrofil memiliki sitoplasma bergranula halus dan ditengahnya terdapat inti bersegmen. Sel eosinofil mempunyai sitoplasma berwarna lebih merah, inti yang terletak memanjang di tepi sel, memiliki granula besar. Sel limfosit memiliki sitoplasma berwarna biru gelap dan inti berbentuk bulat hingga oval. Sel monosit memiliki sitoplasma berwarna biru pucat dan bentuk inti bervariasi ada yang berbentuk seperti ginjal hingga yang berbentuk dua lobus besar.
KUALITAS SPERMATOZOA DAN STRUKTUR HISTOLOGIS GONAD JANTAN TIGA SPESIES IKAN FAMILI CYPRINIDAE DI SUNGAI BANJARAN Habibulah Ahmad Al Gifari; Priyo Susatyo; Atang Atang; Sugiharto Sugiharto
BioEksakta : Jurnal Ilmiah Biologi Unsoed Vol 5 No 1 (2023): BioEksakta
Publisher : Fakultas Biologi Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.bioe.2023.5.1.5203

Abstract

Fisheries researchers reveal that many species of native wild fish are threatened with extinction due to uncontrolled fishing, things that can threaten their existence in nature, one of which is in the Banjaran River. The aims of this study were: to determine the quality of fish spermatozoa, to determine the gonadal maturity index (IKG), to determine the Gonad Maturity Level (TKG), and to determine the histological description of the male gonads in order to obtain data for conservation purposes. The research was conducted by survey method, the sample was taken by purposive random sampling technique. The results of the study during the March-May period showed that the sperm quality of Nilem Fish was best in May, which had a milt volume of 0.21 ml, a spermatozoa concentration of 2.3454 x109 cells/ml, Motility 3, and viability of 82.55%. In Brek Fish the highest volume of milt was found in May, namely 0.25 ml, the highest concentration of spermatozoa was in April 34.465 x109 cells/ml, with viability 88.62%, and motility 2.9. The best quality of Lunjar fish sperm was found in May which had a milt volume of 0.02 ml, spermatozoa concentration of 22.435 x109 cells/ml, 80% viability, and motility 3. All sperm samples were white with a pH of 7-8. Based on TKG and IKG, it can be seen that many Nilem fish are found spawning in April and May, Brek and Lunjar fish are found spawning every month. Histological structure of male gonads in Nilem, Brek and Lunjar fish had the same composition, in immature fish the spermatogenic cell composition observed was dominated by spermatocytes, spermatids, and spermatogonia, in mature fish the gonads were dominated by spermatozoa cells. and spermatids, whereas in post-spawning fish spermatozoa and spermatogonia were found in large number.