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All Journal E-Jurnal Medika Udayana JURNAL BIOMEDIK Berkala Ilmu Kesehatan Kulit dan Kelamin Journal of General Procedural Dermatology and Venereology Indonesia ISM (Intisari Sains Medis) : Jurnal Kedokteran Bali Dermatology and Venereology Journal Neurologico Spinale Medico Chirurgico IJAAM (Indonesian Journal of Anti-Aging Medicine) Journal of Global Pharma Technology
Anak Agung Gde Putra Wiraguna
Department Of Dermatology Faculty Of Medicine, Udayana University
Biochemistry, Genetics & Molecular Biology Health Professions Immunology & microbiology Medicine & Pharmacology Neuroscience Public Health

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Administration of Moringa oleifera seeds ethanol extracts cream increased neovascularization and fibroblasts cell, but did not increase epithelialization in wound healing process of wistar rats (Rattus norvegicus) Golda Bernadet Lemewu; I Gusti Made Aman; Anak Agung Gede Putra Wiraguna
IJAAM (Indonesian Journal of Anti-Aging Medicine) Vol 5 No 2 (2021): Indonesian Journal of Anti-Aging Medicine
Publisher : Fakultas Kedokteran Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36675/ijaam.v5i2.76

Abstract

Introduction: The aging process causes a decrease in organ function like wound healing. Moringa seed extract contains various compounds that can accelerate wound healing. The purpose of this study was to prove that administration of Moringa oleifera can accelerate wound healing process. Methods: This study used a randomized post-test control group design. Subject were 28 rats (Rattus norvegicus), Wistar strain, which were divided into 4 groups (n = 7). The P0(4) and P0(8) group was given placebo for 4 and 8 days, the P1(4) and P1(8) group was given Moringa seed extract cream for 4 and 8 days. All group were given oral amoxicillin 3x10mg/day. Skin samples were biopsied at the wound site. Results: The results showed that the average neovascularization in P0(4) group was 3.57±1.13, P1(4) = 7.71±2.06, P0(8) = 4.14±1.22, and P1(8) = 8.00±1.41 (p < 0.001). The number of fibroblast cells in the P0(4) = 39.4±6.19, P1(4) = 64.3±6.08, P0(8) = 47.0±16.2, and P1(8) = 75.7±17.1 (p < 0.001). The epithelium thickness of the P0(4) = 39.1±15.9 μm, P1(4) = 33.8±3.50 μm, P0(8) = 28.9±11.5 μm, and P1(8) = 38.9±21.5 μm (p> 0.05). The epithelium gap of the P0(4) = 1239±1079 μm, P1(4) = 380±441 μm, P0(8) = 838±1072 μm, and P1(8) = 1153±594 μm (p> 0.05). Conclusion: It can be concluded that administration of Moringa oleifera seeds ethanol extracts cream increased neovascularization and fibroblast cell, but did not increase epithelialization in wound healing process of wistar rats.
Oral Ashitaba Aeaves (Angelica keiskei) Extract Prevented The Increase of Tyrosinase Levels and Total Melanin in The Ultraviolet B­Exposed Guinea Pig (Cavia porcellus) Skin Meiny Linda Setiawan; Wimpie Pangkahila; Anak Agung Gede Putra Wiraguna
IJAAM (Indonesian Journal of Anti-Aging Medicine) Vol 4 No 2 (2020): Indonesian Journal of Anti-Aging Medicine
Publisher : Fakultas Kedokteran Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36675/ijaam.v4i2.100

Abstract

Introduction: UVB rays stimulate the tyrosinase which activates the biosynthesis of melanin and cause skin aging characterized by hyperpigmentation. Ashitaba is a plant rich of chalcone, a flavonoid compound with tyrosinase inhibitor activity. This study aimed to prove that oral Ashitaba (Angelica keiskei) extract prevented the elevation of tyrosinase levels and total melanin in the UVB-exposed guinea pig (Cavia porcellus) skin. Methods: This study used randomized posttest only control group design. The subjects were guinea pigs (Cavia porcellus), male, healthy, local strains, aged 3-4 months, weighing 300-­350 grams, one hybrid, which were divided into 2 groups (n = 18). The first group was the control group (treatment with exposure to ultraviolet B and placebo), the second group was the treatment group (treatment with exposure to ultraviolet B and oral Ashitaba leaves extract of 25 mg/kgBB). After 2 weeks of treatment, tyrosinase level was examined by the ELISA method, while the amount of melanin was examined for Masson­Fontana staining. Results: The mean tyrosinase level in the control group after 2 weeks of treatment was higer (30.64 ± 4.19ng/ml) than on the treatment group (11.47 ± 0.62ng/ml) with (p<0.05). In addition, the average number of melanin in the control group was also higer (19.05 ± 2.53%) than the treatment group (1.85 ± 0.84%) (p <0.05). Conclusion: Based on the results of this study, it can be concluded that oral Ashitaba (Angelica keiskei) extract prevented the increase of tyrosinase levels and total melanin in the UVB-exposed guinea pig (Cavia porcellus) skin.
The application of black garlic (Allium sativum linn) extract cream in preventing the increase level of MMP-1 expression and the decrease of dermal collagen count in the skin of male wistar rats (Rattus norvegicus) exposed to UV-B Rays Balqis Wasliati; Wimpie Pangkahila; Anak Agung Gede Putra Wiraguna
IJAAM (Indonesian Journal of Anti-Aging Medicine) Vol 5 No 2 (2021): Indonesian Journal of Anti-Aging Medicine
Publisher : Fakultas Kedokteran Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36675/ijaam.v5i2.81

Abstract

Introduction: UV-B exposure triggers the activation of Protein-1 (AP-1) which disrupts the integrity of the extracellular matrix by inducing MMP-1. Black garlic causes an increase of antioxidant content namely phenolic, flavonoid (quercetin), and DPPH (IC-50). This study aimed to determine the application of black garlic (Allium sativum linn) extract cream in preventing the increased level of MMP-1 expression and the decrease of dermal collagen count in the skin of male Wistar rats exposed to UV-B rays. Methods: This study employed an experimental Post Test Only Control Group Design using 32 male Wistar rats. The rats that were applied with base cream material and exposed to UV-B rays were called the control group. The rats that were applied with 15% black garlic extract cream and exposed to UV-B rays were called the treatment group. The expression of MMP-1 was examined by immunohistochemistry and the dermal collagen count of the skin using Pico-Sirius-Red staining. Results: The mean expression of MMP-1 of the control group was 28.58% and the treatment group was 8.60% (p <0.001). The mean count of collagen in the control group was 57.53% and the treatment group was 83.80% (p <0.001). There was a significant difference in the MMP-1 expression and the count of collagen between the treatment of the control group and the treatment group. Conclusion: Administration of the cream of 15% black garlic extract was proven to prevent an increase MMP-1 expression and decrease dermal collagen count in the skin of male Wistar rats exposed to UV-B rays.
Ashitaba (Angelica keiskei) Leaves Extract Cream 8% Prevented Tyrosinase and Melanin Elevation in the Ultraviolet B-Exposed Guinea Pig (Cavia porcellus) Skin Erwin Sugito; Wimpie Pangkahila; Anak Agung Gede Putra Wiraguna
IJAAM (Indonesian Journal of Anti-Aging Medicine) Vol 4 No 2 (2020): Indonesian Journal of Anti-Aging Medicine
Publisher : Fakultas Kedokteran Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36675/ijaam.v4i2.99

Abstract

Introduction: Ultraviolet B radiation causes free radical formation, which then activates tyrosinase, an enzyme that plays a role in melanin biosynthesis. The purpose of this study was to prove that Ashitaba (Angelica keiskei) leaves extract cream 8% prevented the increase of tyrosinase levels and the amount of melanin in the ultraviolet B-exposed guinea pig (Cavia porcellus) skin. Methods: This study was conducted with a randomized posttest only control group design. The subjects were guinea pigs (Cavia porcellus), male, healthy, local strains, aged 3-4 months, weighing 300-350 grams, one hybrid, which were divided into 2 groups (n= 18). The control group (ultraviolet B exposure and placebo of basic cream), and the treatment group ultraviolet B exposure and Ashitaba leaves extract cream 8%). After 2 weeks of treatment, tyrosinase levels were examined by the ELISA method, while the amount of melanin was examined using Masson-Fontana staining. Results: The tyrosinase level in the control group was higher than the treatment group. The tyrosinase level of control group was 30.95 ± 3.43 ng/ml while in the treatment group was 11.60 ± 0.34 ng / ml (p <0.05). Similarly, the melanin in the control group was higher than the treatment group. The average number of melanin in the control group was 20.54 ± 2.59% while in the treatment group was 1.96 ± 1.25% (p <0.05). Conclusion: Ashitaba (Angelica keiskei) leaves extract cream 8% prevented the increase of tyrosinase levels and the amount of melanin in the ultraviolet B-exposed guinea pig (Cavia porcellus) skin.
Implantation of Polycaprolactone (PCL) Thread Increased Dermal Collagen of Old Female Wistar Rats (Rattus Norvegicus) More Than Polydioxanone (PDO) Thread Junivan Lindra; Wimpie Pangkahila; Anak Agung Gde Putra Wiraguna
IJAAM (Indonesian Journal of Anti-Aging Medicine) Vol 5 No 2 (2021): Indonesian Journal of Anti-Aging Medicine
Publisher : Fakultas Kedokteran Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36675/ijaam.v5i2.77

Abstract

Introduction: Wrinkles and sagging are the sign of skin aging. Threadlift using polydioxanone (PDO) and polycaprolactone (PCL) can increase the amount of collagen. This study aimed to study which thread is better to induced collagenesis. Methods: This study used a posttest-only control group design. The subjects were 30 Wistar rats, healthy, female, age 16-18 months (human equivalent age 45 years), the body weight of 160-180 grams which were divided into three groups (n = 10). The negative control group (P0) was not given any treatment, the positive control group (P1) was treated with 27G needle pierced, 60 mm with 3 PDO threads horizontally in the dermal layer, treatment group (P2) was treated with 27G needle pierced, 60 mm with 3 PCL threads horizontally in the dermal layer. After 4 weeks, a 5x5mm biopsy was performed on the skin and histopathological preparations were made with Sirius Red staining. Results: The results showed that the amount of collagen in the P0 group was 75.0±1.51, in the P1 group was 82.3 ± 1.49, while in the P2 group was 86.0 ± 1.22 (p <0.001). The LSD test showed that there were differences between the P1 group and the P2 group (p <0.001), where the amount of dermal collagen in the PCL group was higher compared to the PDO group. Conclusion: It can be concluded that the implantation of polycaprolactone (PCL) thread increased dermal collagen of old female Wistar rats (Rattus norvegicus) more than polydioxanone (PDO) thread.
Black rice bran (oryza sativa l. indica) extract cream prevented the increase of dermal matrix metalloproteinase-1 and dermal collagen reduction of male Wistar rats (rattus norvegicus) exposed to ultraviolet-B rays Astried Haryanto; Wimpie Pangkahila; Anak Agung Gde Putra Wiraguna
IJAAM (Indonesian Journal of Anti-Aging Medicine) Vol 4 No 1 (2020): Indonesian Journal of Anti-Aging Medicine
Publisher : Fakultas Kedokteran Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (499.849 KB) | DOI: 10.36675/ijaam.v4i1.48

Abstract

Introduction: The radiation of UV B rays exposure is capable of inducting free radicals to the skin, damaging the dermal collagen and stimulating skin aging. Photoaging prevention may be conducted by utilizing antioxidant and one of the good sources of antioxidant is black rice bran. Therefore the purpose of this research was to assess the effects of topical 35% black rice bran (Oryza sativa L.indica) extract toward dermal Matrix Metalloproteinase-1 (MMP-1) and dermal collagen in male Wistar rats (Rattus norvegicus). Method: A post-test only control group design was conducted using 36 male Wistar rats divided into 2 groups (treatment and control). Both groups were exposed to UV-B rays 3 times a week for 4 weeks but the treatment group received 35% black rice bran extract cream twice a day. The control group was only treated by base cream. Histopathological examination was used to assess MMP-1 expression while Picro Sirius Red staining was used to assess dermal collagen on the rat’s skin. Result: Comparative statistical analysis results for both groups revealed that MMP-1 expression in treatment group was significantly lower than control group (30.94±4.22% vs. 9.57±2.76%; p<0.001). Meanwhile, collagen density was significantly higher in treatment group (83.59±2.84% vs. 58.40±2.69%). Conclusion: Topical application of 35% black rice bran extract cream prevented the increase of MMP-1 expression and dermal collagen reduction. Further study is needed to validate these findings.
Administration of Acacia (Acacia mangium) Leaves Ethanol Extract Cream 5% Prevented The Increase of Melanin Number in Ultraviolet B-Exposed Guinea Pig (Cavia Porcellus) Skin Olivia Julita; Ida Sri Iswari; Anak Agung Gede Putra Wiraguna
IJAAM (Indonesian Journal of Anti-Aging Medicine) Vol 4 No 2 (2020): Indonesian Journal of Anti-Aging Medicine
Publisher : Fakultas Kedokteran Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36675/ijaam.v4i2.101

Abstract

Introduction: Ultraviolet exposure stimulate oxidative stress-mediated melanogenesis, which is the sign of photoaged skin. Acacia (Acacia mangium) leaves extract contains high levels of antioxidants. The aim of this study was to prove the effectiveness of the Acacia leaves ethanol extract cream in preventing an increase of melanin. Methods: This experimental study used randomized posttest only control group design. The research subjects were 36 male guinea pigs (Cavia porcellus), healthy, local strains, aged 3 months, weighing 300-350 grams and had never received prior treatment. Subjects were divided into 2 groups (18 individuals each), namely the control group (treatment with ultraviolet B exposure and basic cream) and the treatment group (treatment with ultraviolet B exposure and acacia leaves ethanol extract cream 5%). After 2 weeks of treatment, the amount of melanin was examined histopathologically using Masson-Fontana staining. Results: The average number of melanin in the control group was 19.33±2.59% while in the treatment group was 2.21±1.25%. Comparative analysis using independent t-test showed that the value of p<0.001. Conclusion: This study suggesting that administration of acacia (Acacia mangium) leaves ethanol extract cream 5% prevented increasing amount of melanin in ultraviolet B-exposed guinea pig (Cavia porcellus) skin. The results showed that there was number of melanin in the control group (19.33±2.59%) were higher than the treatment group (2.21±1.25%) (P<0.001).
Topical administration of 40% soursop (Annona muricata) leaves extract cream prevented melanin elevation in UVB-rays exposed guinea pigs (Cavia porcellus) skin Maya Cesarya Simangunsong; Wimpie Pangkahila; Anak Agung Gede Putra Wiraguna
IJAAM (Indonesian Journal of Anti-Aging Medicine) Vol 5 No 1 (2021): Indonesian Journal of Anti-Aging Medicine
Publisher : Fakultas Kedokteran Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36675/ijaam.v5i1.73

Abstract

Introduction: Chronic exposure to UV light can cause skin abnormalities such as hyperpigmentation due to increased amount of melanin. Soursop (Annona muricata) leaf is a plant that contain flavonoids, polyphenols and antioxidants so that they have the potential as anti- hyperpigmentation. The purpose of this study was to prove that topical administration of 40% soursop leaf extract cream prevents melanin elevation in UVB-rays exposed guinea pigs skin. Methods: This study was an experimental study using randomized posttest only control group design. Subjects were 36 male guinea pigs (Cavia porcellus), brown, adult (3 months old), weighing 300-350 grams which were divided into two groups (n= 18). One group as the negative control group that was exposed to UVB and treated with basic cream (P0), and another group as the treatment group that was exposed to UVB and treated with 40% soursop (Annona muricata) leaves extract cream. The amount of melanin was measured histopathologically using immunohistochemical Masson-Fontana staining. Results: The results showed that the average amount of melanin in control group was 13.756 ± 5.2120, whereas in the 40% soursop (Annona muricata) treatment group was 6.811 ± 2.8105. Comparative analysis using independent t-test showed the p value of <0.001, thus indicating a significant difference between groups after treatment. Conclusion: Based on the results of this study it can be concluded that topical administration of 40% soursop (Annona muricata) leaves extract cream prevented melanin elevation in UVB- rays exposed guinea pigs (Cavia porcellus) skin.
Topical administration of deep sea shark liver oil (desslotm) inhibited mmp-1 expression in rat’s skin exposed to ultraviolet-B Bunge Dianmentari; Gede Ngurah Indraguna Pinatih; Anak Agung Gede Putra Wiraguna
IJAAM (Indonesian Journal of Anti-Aging Medicine) Vol 4 No 1 (2020): Indonesian Journal of Anti-Aging Medicine
Publisher : Fakultas Kedokteran Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (311.196 KB) | DOI: 10.36675/ijaam.v4i1.39

Abstract

Introduction: Squalene is one of strong antioxidants that can inhibit aging proses. However, its effect on the skin aging is still understudied. Therefore, the purpose of this study was to assess the efficacy of topical administration of Deep Sea Shark Liver Oil toward MMP-1 expression in Wistar rat’s (Rattus Norvegicus) skin exposed to UVB. Methods: An experimental randomized post-test only control group study was conducted using a total of 36 rats Wistar rats were used which were divided into 2 groups. The control group received placebo solution while the treatment group was treated by Deep Sea Shark Liver Oil solution. All groups were exposed to UVB with a total dose of 840 mJ/cm² for 4 weeks. Biopsy was conducted for skin sample collection for the examination expression of MMP-1. Results: Immunohistochemistry evaluation showed that the dermal MMP-1 expression in the control group was higher than in control group. According to the percentage of expression analysis, the mean of MMP-1 expression in control group were significantly higher (29.53%) compared to the treatment group (9.72%) (p=0.001). Conclusion: It can be concluded that the topical administration of Deep Sea Shark Liver Oil solution inhibited the expression of MMP-1 in rat’s skin exposed to UVB.
Implantation of Polydioxonone (PDO) Threads Decreased Dorsolumbar Subcutaneous Fat Weight in Obese Female Rats (Rattus Norvegicus) Diana Niti Santoso; Anak Agung Gde Putra Wiraguna; I Gusti Made Aman
IJAAM (Indonesian Journal of Anti-Aging Medicine) Vol 5 No 2 (2021): Indonesian Journal of Anti-Aging Medicine
Publisher : Fakultas Kedokteran Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36675/ijaam.v5i2.78

Abstract

Introduction: Thread-lifts, especially using polydioxanone (PDO), are thought to have targeted lipolysis effect, but have never been empirically proven. The aim of this study was to prove that the implantation of polydioxonone (PDO) threads decrease dorsolumbar subcutaneous fat weight in obese female rats (Rattus norvegicus). Methods: This experimental study used posttest only control group design. The research subjects were 36 healthy Wistar rats, females, aged 16-18 weeks, obese with a body weight of 250-300 grams (Lee index criteria> 0.3). Obesity was induced by high-carbohydrate and high-fat feed. The rats were then divided into 2 groups, the negative control group with catgut thread implant treatment (P0) and the treatment group with polydioxonone (PDO) thread implant treatment (P1). After 28 days the rats were euthanized, skin tissue was collected, subcutaneous fat was searched and separated from the dorsolumbar depots found in the subcutaneous layer in the lumbar spine at the dorsal part of the body, then measured with the Sartorius brand scales. Results: The median weight of dorsolumbar subcutaneous fat in the P0 group was 1.05 (0.80; 1.90) grams, while in P1 group was 0.90 (0.70; 1.10) grams. The results of the comparative analysis with Mann Whitney test showed a p value of 0.016, which means that there was a difference in dorsolumbar subcutaneous fat between groups after 28 days. Conclusion: Implantation of polydioxonone (PDO) threads decreased dorsolumbar subcutaneous fat weight in obese female rats (Rattus norvegicus).
Co-Authors Angela Sandi Tagaroi Rahasia Anne Carolina Kencanawati Astried Haryanto Balqis Wasliati Batan, Putu Nila Wardhani Bunge Dianmentari Chynthia Friama Diana Niti Santoso Dwindi Saptania Erwin Sugito Fatri Idana Fiter, Joni Gde Ngurah Idraguna Pinatih Golda Bernadet Lemewu I Gusti Ayu Agung Dwi Karmila I Gusti Ayu Agung Elis Indira I Gusti Ayu Agung Praharsini I Gusti Made Aman I Ketut Agus Somia Ida Bagus Gede Jayadharma Ida Sri Iswari Junivan Lindra Komang Aditya Yudistira Komang Saputra Yadnya Luh Made Mas Rusyati Made Wardhana Maharani, Made Kusuma Dewi Marrietta Sugiarti Sadeli Martina Windari Maya Cesarya Simangunsong Meiny Linda Setiawan Ni Luh Putu Ratih Vibriyanti Karna Ni Made Dwi Puspawati Ni Wayan Winarti Olivia Julita Pande Agung Mahariski Phery Candres Prima Saraswati Sanjiwani Sudarsa Priskila Ariana Rahel Lisana Debora Hutagalung Somia Sri Subramaniam Stefani Nurhadi Vanessa Vijayamurthy Vijayamurthy, I Dewa Ayu Vanessa Wimpie I Pangkahila Yuziana Yuziana