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All Journal Medicina Journal of Pharmaceutical Science and Application E-Jurnal Medika Udayana JURNAL BIOMEDIK WMJ (Warmadewa Medical Journal) Molecular and Cellular Biomedical Sciences (MCBS) Berkala Ilmiah Kedokteran Duta Wacana Bali Health Journal ISM (Intisari Sains Medis) : Jurnal Kedokteran Indonesian Journal of Clinical Pathology and Medical Laboratory (IJCPML) Lumbung Farmasi : Jurnal Ilmu Kefarmasian IJAAM (Indonesian Journal of Anti-Aging Medicine) Jurnal Sains dan Teknologi Peternakan
I Gusti Made Aman
Department Of Medical Pharmacology, Faculty Of Medicine, Universitas Udayana, Denpasar, Bali
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Dentistry Health Professions Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Neuroscience Public Health Veterinary Other

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Makanan Sebagai Sumber Antioksidan Aman, I Gusti Made
Jurnal Kesehatan Bali Vol 1 No 1 (2017): Bali Health Journal
Publisher : LP2M Institut Ilmu Kesehatan Medika Persada Bali

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Abstract

The aging process will continue, unstoppable, but can be slowed down. One of the factors causing premature aging is the presence of free radicals formed by the body itself or from outside the body. To absorb free radicals, the body forms antioxidants called endogenous antioxidants such as superoxid dismutase, catalase, glutathione peroxidase. The production of these antioxidants decreases with age, thus creating an imbalance between free radicals and antioxidants. When free radicals exceed antioxidants, oxidative stress results in subsequent oxidative damage. To avoid the occurrence of oxidative damage, the body needs antioxidants from the outside (exogenous antioxidants). Foods such as vegetables and fruits are major sources of exogenous antioxidants. Vegetables and fruits contain many flavonoids, tannins, polyphenols. This phytochemical has a potent antioxidant effect. In addition, vegetables and fruits contain vitamins and minerals. Vitamin A, beta carotene, vitamin C and vitamin E are essential nutrients that have antioxidant effects. Minerals such as Zn, Mn, Selenium, Cu are trace elements that are the building blocks of endogenous antioxidants. In addition to these exogenous antioxidants the body can reduce free radicals so as not to cause oxidative stress and can avoid oxidative damage.
ADMINISTRATION OF CACAO BEANS (Theobroma cacao L.) EXTRACTS DECREASE MALONDIALDEHYDE CONCENTRATION AND INCREASE BLOOD NOx CONCENTRATION IN WHITE RAT (Ra"us norvegicus) INDUCED BY PSYCHOSOCIAL STRESS IA, Dewi Wiryanthini; IGM, Aman; NA, Bagiada
Medicina Vol 43 No 3 (2012): September 2012
Publisher : Medicina

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Abstract

Increased production of reactive oxygen species (ROS) causing accumulation of oxidative damage caused by exceeding anti oxidant capacity in the body. Psychological stress as psychosocial stress can induce oxidative stress which subsequently cause increase blood malondialdehyde (MDA) and decrease blood nitrate and nitrite (NOx) concentration as intermediate product of nitric oxide (NO). Cacao beans extracts contained anti oxidant flavanols consist of catechin, epicatechin and procyanidin. The aims of this study is to investigate the effect of cacao beans (Theobroma cacao L.) extracts for decreasing MDA and increasing NOx concentration in white rat (Ra$us norvegicus) blood in stress oxidative state induced by psychosocial stress. It is an experimental study with Pretest-Postest Control Group Design. This study revealed decrease MDA concentration in group P1 (11.47 vs 8.04), P2 (11.92 vs 5.44) and P3 (11.69 vs 2.87) with P = 0.000 and increase NOx concentration in oxidative stress white rat induced by psychosocial stress a[er administration of cacao beans extract in group P1 (1909.83 vs 2085.16), P2 (1912.5 vs 2231.83) and P3 (1871.5 vs 2339.83) with P = 0.005. This study showed that cacao beans extract can inhibit oxidative stress caused by psychosocial stress.
Pemberian Ekstrak Floret Pisang Raja (Musa x paradisiaca) Mencegah Penurunan Kadar Superoksida Dismutase (SOD) pada Hati Mencit (Mus musculus) BALB/c dengan Aktivitas Fisik Berlebih Irtanto, Okky; Pangkahila, Alex; Aman, IGM
Jurnal Biomedik : JBM Vol 9, No 3 (2017): JURNAL BIOMEDIK : JBM
Publisher : UNIVERSITAS SAM RATULANGI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35790/jbm.9.3.2017.17338

Abstract

Abstract: Overtraining accelerates aging due to the excessive production of free radicals that can cause oxidative stress. Banana floret extract contains bioactive compounds with antioxidant capacity which can increase the body's defence to deal with the oxidative stress by increasing the level of superoxide dismutase (SOD). This study was aimed to prove that the banana (Musa x paradisiaca) floret extract could prevent the decrease of superoxide dismutase (SOD) levels in overtraining-induced mice (Mus musculus) BALB/c liver. This was a true experimental study with the post-test only control group design. Subjects were 36 male mice (Mus musculus), BALB/c strain, 12 weeks old, weighing 20-22 g, which were divided into two groups with 18 mice each. The control group (P0) was treated with a placebo of 1 ml aquadest and overtrained for 14 days meanwhile the treatment group (P1) was treated with banana (Musa x paradisiaca) floret extract of 400 mg/kgBW/day and overtrained for 14 days. The results showed that after 14-day treatment, the mean SOD level in the liver tissue of the P0 group was 568.82±9.558 U/mg protein whereas in the P1 group was 588.37±10.629 U/mg protein (P < 0.01). The t-independent test showed a t value of -5.804 and a P value of 0.000 which indicated that after treatment, the levels of SOD in liver tissue of both groups were significantly different. Conclusion: Banana (Musa x paradisiaca) floret extract could prevent the decrease of superoxide dismutase (SOD) levels in the liver tissue of overtraining-induced mice (Mus musculus) BALB/c.Keywords: banana floret, SOD, liver, overtrainingAbstrak: Aktivitas fisik berlebih mempercepat penuaan karena meningkatkan produksi radikal bebas yang dapat menyebabkan stres oksidatif. Ekstrak floret pisang mengandung senyawa bioaktif dengan kapasitas antioksidan yang dapat meningkatkan pertahanan tubuh dalam menghadapi stres oksidatif melalui peningkatan kadar superoksida dismutase (SOD). Penelitian ini bertujuan untuk membuktikan bahwa pemberian ekstrak floret pisang raja (Musa x paradisiaca) dapat mencegah penurunan kadar SOD pada hati mencit (Mus musculus) BALB/c dengan aktivitas fisik berlebih. Jenis penelitian ialah eksperimental murni dengan post test only control group design. Subjek penelitian ialah 36 ekor mencit (Mus Musculus) BALB/c, jantan, berumur 12 minggu, berat badan 20-22 gr, yang dibagi menjadi dua kelompok masing-masing berjumlah 18 ekor mencit. Kelompok kontrol (P0) diberikan plasebo berupa aquadest sebanyak 1 ml dengan aktivitas fisik berlebih selama 14 hari, dan kelompok perlakuan (P1) diberikan ekstrak floret pisang raja (Musa x paradisiaca) dosis 400 mg/kgBB mencit per hari dicampur aquadest hingga 1 ml dengan aktivitas fisik berlebih selama 14 hari. Hasil penelitian menunjukkan rerata kadar SOD jaringan hati pada kelompok kontrol (P0) sesudah perlakuan (post-test) ialah 568,82±9,558 U/mg protein, sedangkan pada kelompok perlakuan (P1) ialah 588,37± 10,629 U/mg protein. Analisis kemaknaan dengan T-Independent mendapatkan nilai t= -5,804 dan nilai P = 0,000 yang menunjukkan bahwa sesudah perlakuan (post-test), kadar SOD jaringan hati pada kedua kelompok berbeda sangat bermakna. Simpulan: Ekstrak floret pisang raja (Musa x paradisiaca) dapat mencegah penurunan kadar SOD pada hati mencit (Mus musculus) BALB/c dengan aktivitas fisik berlebih.Kata kunci: floret pisang raja, SOD, hati, aktivitas fisik berlebih
PEMBERIAN EKSTRAK DAUN GANDARUSA (JUSTICIA GENDARUSSA BURM. F.) MENURUNKAN KADAR F2-ISOPROSTAN URIN TIKUS WISTAR (RATTUS NORVEGICUS) JANTAN YANG DIINDUKSI LATIHAN FISIK BERLEBIH Akpriyanti, DG Ika; Pangkahila, Wimpie; Aman, IGM
JURNAL BIOMEDIK : JBM Vol 9, No 3 (2017): JURNAL BIOMEDIK : JBM
Publisher : UNIVERSITAS SAM RATULANGI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35790/jbm.9.3.2017.17337

Abstract

Abstract: Excessive physical activity increases the consumption of oxygen which can result in increased levels of reactive oxygen species (ROS) causing oxidative stress characterized by the elevation of F2-isoprostane as a byproduct of lipid peroxidation. Willow (Justicia gendarussa Burm. f.) has an antioxidant activity that is able to inhibit and break the chain reaction of free radicals and prevent lipid peroxidation. This study was aimed to prove that willow leaf extract could inhibit the elevation of F2-isoprostane levels in male Wistar rats (Rattus norvegicus) urin induced by excessive physical training. This was a true experimental study using 16 male Wistar rats (Rattus norvegicus), aged 2-3 months, weighing 180-200 g as subjects. The control group (P0) was treated with a placebo and excessive physical training, and the treatment group (P1) was treated with the willow leaf extract of 200mg/rat/day and excessive physical training. Before and after 14 days of treatment, the urine samples were collected and their levels of F2-isoprostane 8-isoPGF2? were examined by using enzyme immunoassay kit (EIA). The results showed that the mean level of F2-isoprostane in the control group (P0) before treatment (pretest) was 5.20±0.483 ng / mL while in the treatment group (P1) was 5.46±0.655 ng / mL (P > 0.05). On the other hand, after treatment for 7 days (post-test), the mean level of F2-isoprostane in the control group (P0) was 5.34±0.476 ng/mL and in the treatment group (P1) was 3.61±0.389 ng/mL (P < 0.01). Conclusion: The extract of willow (Justicia gendarussa Burm. f.) leaf could inhibit the elevation of F2-isoprostane levels in male Wistar rats (Rattus norvegicus) urin induced by excessive physical training.Keywords: willow leaf, F2-isoprostane, excessive physical trainingAbstrak: Aktivitas fisik berlebih akan meningkatkan komsumsi oksigen yang dapat mengakibatkan peningkatan kadar reactive oxygen species (ROS) sehingga menyebabkan stres oksidatif yang ditandai dengan meningkatnya F2-isoprostan sebagai salah satu produk peroksidasi lipid. Tanaman gandarusa (Justicia gendarussa Burm. f.) mengandung antioksidan yang dapat mencegah reaksi peroksidasi lipid. Penelitian ini bertujuan untuk membuktikan bahwa pemberian ekstrak daun gandarusa dapat menurunkan kadar F2-isoprostan urin tikus Wistar (Rattus norvegicus) jantan yang diinduksi latihan fisik berlebih. Jenis penelitian ialah eksperimental dengan subjek 16 ekor tikus (Rattus norvegicus), galur Wistar, sehat, berumur 6 bulan, berat badan 180-200 gr, dengan kadar F2-isoprostan >2ng/mL. Kelompok kontrol (P0) diberikan pelatihan fisik berlebih dan plasebo aquadest 2ml sedangkan kelompok perlakuan (P1) diberikan pelatihan fisik berlebih dan ekstrak daun gandarusa dosis 200mg/ekor/hari. Sebelum dan setelah 14 hari perlakuan, sampel urin ditampung dan diperiksa kadar F2-isoprostan menggunakan 8-isoPGF2? enzyme immunoassay kit (EIA) dari assay design untuk data pretest dan post-test. Hasil penelitian menunjukkan rerata kadar F2-isoprostan pada kelompok P0 sebelum perlakuan (pretest) ialah 5,20±0,483 ng/mL, sedangkan pada kelompokP1 ialah 5,46±0,655 ng/mL (P > 0,05). Setelah perlakuan selama 14 hari (post-test), rerata kadar F2-isoprostan pada kelompok P0 ialah 5,34±0,476 ng/mL dan pada kelompok P1 ialah 3,61±0,389 ng/mL (P < 0,01). Simpulan: Ekstrak daun gandarusa (Justicia gendarussa burm. f.) dapat menurunkan kadar F2-isoprostan urin tikus Wistar (Rattus norvegicus) jantan yang diinduksi latihan fisik berlebih.Kata kunci: daun gandarusa, F2-isoprostan, pelatihan fisik berlebih
PEMBERIAN BISPHENOL A (BPA) ORAL DAPAT MENURUNKAN KADAR TESTOSTERON PADA TIKUS (RATTUS NORVEGICUS) JANTAN GALUR SPRAGUE DAWLEY ., Nuriyah; Aman, IGM; Pangkahila, Wimpie
JURNAL BIOMEDIK : JBM Vol 9, No 2 (2017): JURNAL BIOMEDIK : JBM
Publisher : UNIVERSITAS SAM RATULANGI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35790/jbm.9.2.2017.16355

Abstract

Abstract: Bisphenol A (BPA) has the structural and physiological activity of estrogen and acts as an endocrine disruptor. This study was aimed to prove that oral BPA could decrease testosterone levels in male Sprague Dawley rats. This was an experimental study with a randomized pretest-posttest control group design using 14 rats (Rattus norvegicus), male, Sprague Dawley strain, 2-3 months old, as subjects. Rats were divided into two groups with 7 rats each. The control group (P0) was treated with placebo for 14 days and the treatment group (P1) was treated with oral bisphenol A (BPA) single dose of 400mg/kg/day for 14 days (P1). At before and after 14-day treatment, blood was drawn through the canthus medial of orbital sinus to obtain the pretest and posttest data. Testosterone levels were examined by using ELISA. The results showed that before treatment (pretest), the mean testosterone level in P0 group was 8.29±4.68 nmol/L, while in P1 group was 7.39±1.45 nmol/L (P > 0.05). After treatment for 14 days, the mean testosterone level in P0 group was 7.40±1.37 nmol/L, while in P1 group was 3.36±1.26 nmol/L (P < 0.01). The statistical analysis showed that there was no difference in testosterone levels in P0 group (P > 0.05), meanwhile there was a very significant decrease of testosterone levels in P1 group (P < 0.01) after the treatment of BPA 400mg/kg/day for 14 days. Conclusion: Oral bisphenol A (BPA) could decrease testosterone levels in male rats (Rattus norvegicus) of Sprague Dawley strain.Keywords: bisphenol A (BPA), testosteroneAbstrak: Bisphenol A (BPA) memiliki aktifitas hormon estrogen sehingga jika masuk ke dalam tubuh dapat menjadi endocrine disruptor. Penelitian ini bertujuan untuk membuktikan pemberian BPA oral dapat menurunkan kadar testosteron pada tikus (Rattus norvegicus) jantan galur Sprague Dawley. Jenis penelitian ialah eksperimental dengan randomized pretest-postest control group design yang menggunakan 14 ekor tikus (Rattus norvegicus) jantan, galur Sprague Dawley, berumur 2-3 bulan, yang terbagi menjadi 2 (dua) kelompok masing-masing berjumlah 7 ekor tikus. Kelompok kontrol (P0) diberikan plasebo 14 hari dan kelompok perlakuan (P1) diberi BPA dosis tunggal 400mg/kgBB/hari selama 14 hari. Saat sebelum dan sesudah perlakuan selama 14 hari, darah diambil melalui chantus medial orbitalis untuk pemeriksaan pretest dan postest dan kadar testosteron diperiksa dengan metode ELISA. Hasil penelitian menunjukkan bahwa sebelum perlakuan (pretest), rerata kadar testosteron kelompok P0 8,29±4,68 nmol/L, sedangkan pada kelompok P1 7,39±1,45 nmol/L (P > 0,05). Setelah perlakuan selama 14 hari, rerata kadar testosteron kelompok P0 ialah 7,40±1,37 nmol/L dan pada kelompok P1 3,36±1,26 nmol/L (P < 0,01). Analisis efek perlakuan menunjukkan tidak terjadi penurunan kadar hormon testosteron pada kelompok P0 (P > 0,05), sedangkan penurunan bermakna terdapat pada kelompok P1 (P < 0,01). Simpulan: Pemberian bisphenol A (BPA) oral dapat menurunkan kadar testosteron pada tikus (Rattus norvegicus) jantan galur Sprague Dawley.Kata kunci: bisphenol A (BPA), testosteron
Pemberian ekstrak daging ayam broiler meningkatkan berat badan dan berat lemak viseral serta meningkatkan produksi rambut pada anak tikus (Rattus norvegicus) jantan galur Wistar Syafii, Corry P.; Pangkahila, Wimpie; Aman, IGM
Jurnal Biomedik : JBM Vol 9, No 2 (2017): JURNAL BIOMEDIK : JBM
Publisher : UNIVERSITAS SAM RATULANGI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35790/jbm.9.2.2017.16356

Abstract

Abstract: Despite the estrogen and progesterone content found in commercialy prepared fried chicken, these types of restaurants are proliferating worldwide. This study was aimed to prove that oral broiler meat extract increased body weight, visceral fat mass, and hair follicles in young male Wistar rat (Rattus norvegicus). This was a completely randomized experimental study with the post-test only control group design. Subject were 36 healthy male rats (Rattus norvegicus), Wistar strain, aged 1.5 months (equivalent to human of 13 years old), weighing 80-150 g, divided into two groups of 18 rats each. The control group (P0) was treated with distilled water as placebo for 21 days and the treatment group (P1) was treated with oral broiler meat extract of 3.6 g/rat/day for 21 days. After 21-day treatment, rats were weighed and were terminated for observation of their visceral fat mass meanwhile their skin tissues were examined histologically for the numbers of hair follicles. The results showed that the mean body weight after 21-day treatment in the P0 group was 173.94±4.976 g, whereas in the P1 group was 195.94±4.869 g (P < 0.01). The mean visceral fat mass after 21 days of treatment in the P0 group was 0.356±0.0784 g whereas in the P1 group was 0.567±0.1138 g (P < 0.01). The mean number of hair follicles after 21-day treatment in the P0 group was 23.78±1.629 follicles at four fields of view meanwhile in the P1 group was 31.56±2.332 follicles at four fields of view (P < 0.01). Conclusion: Oral broiler meat extract could increase body weight, visceral fat mass, and hair follicles in young male rat (Rattus norvegicus) of Wistar strain.Keywords: broiler meat, body weight, visceral fatAbstrak: Umumnya daging ayam broiler mengandung estrogen dan progesteron namun restoran cepat saji yang menyajikan menu ayam goreng olahan telah menularluas di seluruh dunia. Penelitian ini bertujuan untuk membuktikan bahwa pemberian ekstrak daging ayam broiler dapat meningkatkan berat badan, berat lemak viseral, dan pertumbuhan rambut anak tikus (rattus norvegicus) jantan galur Wistar. Jenis penelitian ialah eksperimental completely randomized dengan posttest only control group design. Subyek penelitian ialah 36 ekor tikus putih (Rattus norvegicus) jantan, galur Wistar, sehat, umur 1,5 bulan (sesuai dengan anak usia 13 tahun), dengan berat badan 80-150 gr yang dibagi menjadi 2 (dua) kelompok masing-masing berjumlah 18 ekor tikus. Kelompok kontrol (P0) diberikan plasebo berupa aquades selama 21 hari, dan kelompok perlakuan (P1) diberikan ekstrak daging ayam broiler dosis 3,6 gr/ekor/hari selama 21 hari. Setelah 21 hari perlakuan seluruh tikus diukur berat badannya, dan tikus diterminasi untuk pemeriksaan berat lemak viseral dan organ kulit diproses untuk pemeriksaan folikel rambut. Hasil penelitian menunjukkan rerata berat badan sesudah 21 hari perlakuan kelompok P0 ialah 173,94±4,976 gr, sedangkan pada kelompok P1 195,94±4,869 gr (P < 0,01). Rerata berat lemak viseral sesudah 21 hari perlakuan kelompok P0 ialah 0,356±0,0784 gr, sedangkan pada kelompok P1 0,567±0,1138 gr (P < 0,01). Rerata jumlah folikel rambut setelah 21 hari perlakuan pada kelompok P0 ialah 23,78±1,629 folikel pada 4 lapang pandang, sedangkan pada kelompok P1 31,56±2,332 folikel pada 4 lapang pandang (P < 0,01). Simpulan: Ekstrak daging ayam broiler dapat meningkatkan berat badan, berat lemak viseral, dan pertumbuhan rambut anak tikus (Rattus norvegicus) jantan galur Wistar.Kata kunci: daging ayam broiler, berat badan, lemak viseral
Pemberian Bisphenol A (BPA) Oral Menurunkan Kadar Superoksida Dismutase dan Meningkatkan Kadar F2-isoprostan pada Tikus Putih (Rattus norvegicus) Jantan Galur Sprague Dawley Sulaiman, Yogie; Aman, IGM; Pangkahila, Wimpie
Jurnal Biomedik : JBM Vol 9, No 3 (2017): JURNAL BIOMEDIK : JBM
Publisher : UNIVERSITAS SAM RATULANGI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35790/jbm.9.3.2017.17336

Abstract

Abstract: Bisphenol A (BPA), commonly found in plastic packaging and cans, can induce oxidative stress. This study was aimed to prove that oral BPA could decrease superoxide dismutase (SOD) and increase F2-isoprostan levels in male Sprague Dawley rats. This was an experimental study using randomized pretest-posttest control group design. There were 14 rats (Rattus norvegicus), male, Sprague Dawley strain, 8-10 weeks old, as subjects. Rats were divided into two groups with 7 rats each. The control group (P0) was treated with placebo for 21 days and the treatment group (P1) was treated with oral BPA dose of 400mg/kg for 21 days. Before and after treatment for 21 days, blood was drained through the canthus medial sinus orbital and urine was collected as pretest and posttest data. SOD and F2-isoprostan levels were then examined by using ELISA. The results showed that before treatment (pretest), the mean SOD level in P0 group was 42.51±3.127 U/mL and in P1 group was 41.87±2.477 U/mL (P > 0.05). The mean F2-isoprostane level in P0 group was 3.18±0.599 ng/ml and in P1 group was 3.22±0.489 ng/ml (P > 0.05). However, after treatment for 21 days (posttest), the mean level of SOD in P0 group was 43.52±3.472 U/mL and in P1 group was 31.84±2.563 U/mL (P <0.01). The mean level of F2-isoprostane in P0 group was 2.94±0.500 ng/ml and in P1 group was 4.47±0.241 ng/ml (P < 0.01). The analysis of the treatment effect showed significant decreased level of SOD and increased level of F2-isoprostane in P1 group (P < 0.01). Conclusion: Oral bisphenol A could decrease superoxide dismutase (SOD) and increase F2-isoprostan levels in male Sprague dawley rats.Keywords: bisphenol A (BPA), SOD, F2-isoprostane, Sprague Dawley ratsAbstrak: Bisphenol A (BPA) banyak ditemukan dalam kemasan plastik dan kaleng dan dapat menyebabkan peningkatan radikal bebas. Penelitian ini bertujuan untuk membuktikan pemberian BPA oral dapat menurunkan kadar superoksida dismutase (SOD) dan meningkatkan kadar F2-isoprostan pada tikus (Rattus norvegicus) jantan galur Sprague Dawley. Jenis penelitian ialah eksperimental dengan randomized pretest-postest control group design yang menggunakan 14 ekor tikus (Rattus norvegicus) jantan, galur Sprague Dawley, berumur 8-10 minggu, yang terbagi menjadi 2 (dua) kelompok masing-masing berjumlah 7 ekor tikus. Kelompok kontrol (P0) diberikan plasebo selama 21 hari dan kelompok perlakuan (P1) diberikan BPA dosis 400mg/kgBB selama 21 hari. Sebelum dan sesudah perlakuan selama 21 hari, darah diambil melalui chantus medial orbitalis dan urin dikumpulkan untuk pemeriksaan pretest dan postest. Kadar SOD dan F2-isoprostan diperiksa dengan metode ELISA. Hasil penelitian menunjukkan bahwa sebelum perlakuan (pretest), rerata kadar SOD kelompok P0 42,51±3,127 U/mL dan kelompok P1 41,87±2,477 U/mL (P > 0,05). Rerata kadar F2-isoprostan kelompok P0 3,18±0,599 ng/ml dan kelompok P1 3,22±0,489 ng/ml (P > 0,05). Setelah perlakuan selama 21 hari (posttest), rerata kadar SOD kelompok P0 43,52±3,472 U/mL dan kelompok P1 31,84±2,563 U/mL (P < 0,01). Rerata kadar F2-isoprostan kelompok P0 2,94±0,500 ng/ml dan kelompok P1 4,47±0,241 ng/ml (P < 0,01). Analisis efek perlakuan menunjukkan terjadi penurunan kadar SOD dan peningkatan kadar F2-isoprostan yang bermakna pada kelompok P1 (P < 0,01). Simpulan: Bisphenol A oral dapat menurunkan kadar superoksida dismutase dan meningkatkan kadar F2-isoprostan pada tikus galur Sprague Dawley jantan.Kata kunci: BPA, SOD, F2-Isoprostan, tikus Sprague Dawley
Pemberian makanan pengganti cair Nutrisure Gold® tidak meningkatkan kadar hormon estrogen tetapi meningkatkan kadar hormon progesteron pada anak tikus putih (Rattus norvegicus) galur Wistar betina Wardani, Setia; Pangkahila, Wimpie; Aman, IGM
Jurnal Biomedik : JBM Vol 9, No 1 (2017): JURNAL BIOMEDIK : JBM Suplemen
Publisher : UNIVERSITAS SAM RATULANGI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35790/jbm.9.1.2017.15376

Abstract

Abstract: One of the factors that influences aging process is hormonal imbalance that can occur due to the intake of food or drink containing hormone. Children aged more than 1 year with difficulty in eating are usually given Nutrisure Gold®. Analysis has shown that Nutrisure Gold® contains estrogen dan progesterone. This study was aimed to prove that Nutrisure Gold® could increase estrogen and progesterone levels in Wistar female rats. This was an experimental study with the post test only control group design. Subjects were 36 female Wistar rats (Rattus norvegicus) aged 21 days divide into 2 groups, each of 18 rats. The control group was given white rice 3.11 g and aqua bidest meanwhile the treatment group was given Nutrisure Gold® 0.93 g three times a day for 28 days. The results showed that the average estrogen level in the control group was 44.26±11.49 pg/ml and of the treatment group was 45.30±7.66 pg/ml. The average progesterone level in the control group was 22.43±3.62 ng/ml and of the treatment group was 27.44±2.28 ng/ml. Data of estrogen level were analyzed with t-independent test that showed t = -0.320 and P = 0.751 (P >0.05) meanwhile of progesterone level showed t = -4.978 and P = 0.000 (P <0.05). Conclusion: After treatment, the average estrogen levels in both groups did not differ significantly meanwhile the average progesterone levels in both groups showed significant difference.Keywords: liquid replacement meal, estrogen, progesteroneAbstrak: Salah satu faktor yang memengaruhi proses penuaan ialah ketidak seimbangan hormon yang dapat terjadi antara lain akibat pemberian asupan makanan atau minuman yang mengandung hormon. Anak-anak berusia satu tahun ke atas dengan masalah sulit makan sering diberikan makanan pengganti cair Nutrisure Gold®. Analisis menunjukkan bahwa Nutrisure Gold® mengandung hormon estrogen dan progesteron. Penelitian ini bertujuan untuk membuktikan bahwa pemberian Nutrisure Gold® meningkatkan kadar hormon estrogen dan progesteron pada tikus putih galur Wistar betina umur 21 hari. Jenis penelitian ialah eksperimental dengan post test only control group design, Subyek penelitian ialah 36 ekor tikus putih (Rattus norvegicus) galur Wistar betina umur 21 hari yang dibagi atas 2 kelompok, masing-masing kelompok terdiri dari 18 ekor. Kelompok kontrol diberikan nasi putih 3,11 gr dan aquabides sedangkan kelompok perlakuan diberikan Nutrisure Gold® 0,93 gr 3 kali sehari selama 28 hari. Hasil penelitian menunjukkan rerata kadar estrogen kelompok kontrol 44,26±11,49 pg/ml dan rerata kelompok perlakuan 45,30±7,66 pg/ml. Rerata kadar progesteron kelompok kontrol 22,43±3,62 ng/ml dan rerata kelompok perlakuan 27,44±2,28 ng/ml. Analisis kemaknaan kadar estrogen dengan uji t-independent menunjukkan t = -0.320 dan p = 0,751 (P >0,05). Analisis kemaknaan kadar progesteron dengan uji t independent menunjukkan nilai t = -4,978 dan p = 0.000 (P < 0,05). Simpulan: Setelah diberi perlakuan, rerata kadar estrogen pada kedua kelompok tidak berbeda bermakna tetapi rerata kadar progesteron pada kedua kelompok menunjukkan perbedaan bermakna.Kata kunci: makanan pengganti cair, estrogen, progesteron
Efek Pemberian Virgin Coconut Oil (Cocos nucifera) terhadap Dislipidemia pada Tikus Putih (Rattus norvegicus) Jantan Galur Wistar yang Diberi Diet Tinggi Kolesterol Augus Venty; I Gusti Made Aman; Wimpie Pangkahila
WMJ (Warmadewa Medical Journal) Vol 1 No 2 (2016): November 2016
Publisher : Warmadewa University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22225/wmj.1.2.28.58-65

Abstract

Dislipidemia adalah kelainan metabolisme lipid yang ditandai peningkatan kolesterol total, kolesterol LDL, trigliserida diatas nilai normal serta penurunan kolesterol HDL di dalam darah. Virgin coconut oil (VCO) mengandung 92% lemak jenuh sehingga dikaitkan dengan peningkatan resiko penyakit kardiovaskular. Tidak semua asam lemak jenuh buruk bagi kesehatan. VCO terdiri dari medium chain triglycerides (MCT) dan komponen polifenol bekerja secara sinergis dalam mencegah dislipidemia. Penelitian ini bertujuan untuk mengetahui kegunaan VCO sebagai alternatif untuk mencegah dislipidemia pada tikus putih jantan galur wistar yang diberi diet tinggi kolesterol. Penelitian ini adalah penelitian eksperimental murni dengan randomized post-test only control group design menggunakan 36 ekor tikus putih jantan. Semua sampel diberikan diet tinggi kolesterol, lalu dibagi menjadi 2 kelompok secara random yaitu kelompok kontrol yang diberikan plasebo berupa akuades sebanyak 0,8 ml/ hari dan kelompok perlakuan yang diberikan VCO sebanyak 0,8 ml/ 200 gram berat badan/ hari selama 28 hari. Hasil penelitian ini menunjukkan bahwa rerata kolesterol total kelompok perlakuan lebih rendah dari kelompok kontrol (113,49±4,31 mg/dl vs 222,61±6,41 mg/dl) (p<0,05). Rerata trigliserida kelompok perlakuan lebih rendah dari kelompok kontrol (91,46±5,56 mg/dl vs 153,51±5,40 mg/dl) (p<0,05). Rerata kolesterol LDL kelompok perlakuan lebih rendah dari kelompok kontrol (37,94±3,32 mg/dl vs 71,25±2,76  mg/dl) (p<0,05). Rerata kolesterol HDL kolesterol total kelompok perlakuan lebih tinggi dari kelompok kontrol (41,62±1,58 mg/dl vs 25,02±2,59 mg/dl) (p<0,05). Penelitian ini menyimpulkan bahwa virgin coconut oil mencegah dislipidemia pada tikus jantan galur wistar yang diberi diet tinggi kolesterol.Kata Kunci: virgin coconut oil, profil lipid, dislipidemia. [The Effect of Virgin Coconut Oil (Cocos nucifera) to Dyslipidemia in Male Wistar Rats (Rattus norvegicus) Fed with High Cholesterol Diet].Dyslipidemia is a lipid metabolism disorder followed by high total cholesterol level, high LDL cholesterol level, high tryglyceride level and low HDL cholesterol level. Virgin coconut oil (VCO) contains 92% saturated fat that is associated with an increased risk of cardiovascular disease. Not all saturated fatty acids are bad for health. VCO consist of medium chain triglycerides (MCTs) and polyphenol components that work synergistically in preventing dyslipidemia. This research was aimed to determine the usefulness of VCO as an alternative to prevent dyslipidemia in male wistar rats fed with high cholesterol diet. This study was a true experimental research with randomized post-test only control group design using 36 male rats. All samples were given a high cholesterol diet and were divided into 2 groups randomly: a control group were given a placebo (distilled water) 0,8 ml/ day and a treatment group were given VCO 0,8 ml/ 200gram weight/ day for 28 days. The results showed that the treatment group had a significantly lower total cholesterol levels compared to the control group (113,49±4,31 mg/dl vs 222,61±6,41 mg/dl) (p<0,05). The treatment group had a significantly lower triglycerides levels compared to the control group (91,46±5,56 mg/dl vs 153,51±5,40 mg/dl) (p<0,05). The treatment group had a significantly lower LDL cholesterol levels compared to the control group (37,94±3,32 mg/dl vs 71,25±2,76  mg/dl) (p<0,05). The treatment group had a significantly higher HDL cholesterol levels compared to the control group (41,62±1,58 mg/dl vs 25,02±2,59 mg/dl) (p<0,05). This research concluded that virgin coconut oil was proved in preventing dyslipidemia in male wistar rats fed with high cholesterol diet.Keywords: virgin coconut oil, lipid profile, dyslipidemia.
Pola penggunaan obat tetes mata pada karyawan IT penderita computer vision syndrome di lingkungan PT. Telekomunikasi Indonesia, Tbk. Cabang denpasar Dinda Paramaningtyas Sudibya; I Gusti Ayu Artini; I Gusti Made Aman
E-Jurnal Medika Udayana Vol 7 No 5 (2018): E-Jurnal Medika Udayana
Publisher : Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (223.902 KB)

Abstract

Generally 80% office works are done using computers, although computer usage actually can lead to some problems. The collective physical disorder suffered by computer users are called Computer Vision Syndrome (CVS), which also suffered by approximately 88-90% computer users. The aim of this study was to define eye drops usage pattern in IT staffs in Denpasar office branch of PT. Telekomunikasi Indonesia, Tbk who suffer CVS. Descriptive cross-sectional is the method used in this research by doing direct observation, and also using questionnaire as the medium given to study subject who met the inclusions and exclusions criterion. Data from study subject then proceed using SPSS for descriptive statistic. From 60 patients who met the criterion, 40 subjects (66.7%) suffer CVS. According to the education level, we found the highest number in subject with good knowledge (51.7% in CVS group and 25% in non-CVS group). According to the eye condition, researchers found that 21 (35%) subjects in CVS group have good behavior, also in non-CVS subjects (18.3%). According to the regularity of eye drops usage, we found the highest number in “regular enough” category (58.3%) and the lowest in “very regular” (3.3%) in CVS group, while in non-CVS group, the highest was found in “regular enough” (28.3%) and the lowest in “less regular” (5%). According to the eye drops usage patterns, we found the highest number in “good” category (38.3%) for CVS group, while in non-CVS group was in “less” category (25%). Incident prevalence of CVS in this study was different with the previous study and probably this is because of the purposive sampling performed in this study. Study subject in this study were majority have a high education background, therefore this also affected subject’s behavior towards eye health, regularity and pattern of eye drops usage. Keywords: Computer Vision Syndrome (CVS), computer, eye drops
Co-Authors ., Nuriyah A.A.G.P. Wiraguna AAGP Wiraguna Agung Nova Mahendra Akpriyanti, DG Ika Ana Yulia Anak Agung Gde Putra Wiraguna Anak Agung Gede Budhiarta Arina Papita Simanungkalit Augus Venty Bagiada NA Bagus Komang Satriyasa Bissi Herliane Citra Anissa Ayu Cynthia Sofyanti Sugiharto Delly Dahlia Dewa Ayu Made Adnya Swari Dian Andriani Dian Andrieany Husodo Diana Niti Santoso Dinda Paramaningtyas Sudibya Eveline Margo Fanny Rustandi Felix Kusmana Ferbian Milas Siswanto Gde Ngurah Idraguna Pinatih Gede Gunawan Mahardika Golda Bernadet Lemewu Hayatul Firi Hayyu Kusumaningtyas Heidy Dwi Nadia Hendrik Danico Hendro Sukoco Heny Widiyowati S I Gusti Ayu Artini I Ketut Suada I Nyoman Adiputra I Putu Eka Kusuma Yasa I Wayan Martadi Santika I Wayan Putu Sutirta Yasa I Wayan Weta I.A.A. Widhiartini Ida Ayu Dewi Wiryanthini Ida Sri Iswari Irtanto, Okky J. A. Pangkahila Kadek Aprilia Sukma Dewi Ken Andrina Anindita Ketut Widyani Astuti Laura Indriana Liya Rosdiana Made Dio Lokantara Made Oka Negara Manus, Widya Christine Maria Eka Patri Yuliati Maulidha Suzi Annisa Melita Hidajat Nadia Anastasia Ni Nyoman Ayu Dewi Ni Nyoman Ayu Dewi Ni Nyoman Ayu Dewi Ni Wayan Sucindra Dewi Noviyanti Situmorang Olivia Olivia Priskila Ariana Putri, Dhiancinantyan Windydaca Brata Putu Ayu Melati Widyasari Setia Wardani Silvia Irani Sriwiyanti Sriwiyanti Suarni . Sulaiman, Yogie Susianingsih Murni Hartati Syafii, Corry P. Valentine Retny Shiajanto Wimpie I Pangkahila Yuliana Tjua