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All Journal Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences The Journal of Experimental Life Sciences (JELS) Natural B Berkala Penelitian Hayati Bioscientiae, Jurnal ilmu ilmu Biologi Makara Journal of Science
Dian Siswanto
Department Of Biology, Faculty Of Mathematics And Natural Science, Universitas Brawijaya, Malang, Indonesia
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Environmental Science Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Neuroscience Veterinary

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Mangrove Conservation in East Java: The Ecotourism Development Perspectives Hakim, Luchman; Siswanto, Dian; Makagoshi, Nobukazu
Journal of Tropical Life Science Vol 7, No 3 (2017)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.07.03.14

Abstract

An analysis of the role of mangrove ecosystems in tourism was undertaken in order to build a strategy for mangrove conservation and conceptualize sustainable mangrove-based tourism development in East Java, Indonesia. The results of the present study suggest that mangroves could be used as nature-based tourism destinations. While tourism in mangrove areas in East Java clearly contributes to mangrove conservation, it still lacks a mangrove tour program, in which it is important to deliver the objectives of ecotourism. For the sustainable use of mangrove biodiversity as a tourist attraction, it is essential to know the basic characteristics of mangroves and establish mangrove tourism programs which are able to support a conservation program. It is also crucial to involve and strengthen the participation of local communities surrounding mangrove areas. The involvement of local wisdom could increase the sustainability of mangrove ecosystems.
Improvement of Trimethylamine Uptake by Euphorbia milii: Effect of Inoculated Bacteria Siswanto, Dian; Thiravetyan, Paitip
Journal of Tropical Life Science Vol 6, No 2 (2016)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.06.02.11

Abstract

In the last few years, a great emphasis has been placed on phytoremediation of indoor air pollution studies. However, limited work has been addressed to observe the bacteria potential to assist the phytoremediation process of trimethylamine (TMA). In this work, the ability of 4 different bacteria for individual TMA removal and IAA production were observed. In addition, the enhancement of TMA removal efficiency by Euphorbia milii with various inoculating bacteria were investigated. Bacillus thuringiensis, Citrobacter amalonaticus Y19, Bacillus nealsonii, and white colony-soil bacteria (WCSB) were able to absorb TMA and produce IAA individually. B. thuringiensis and C. amalonaticus Y19 were the two most effective bacteria to improve TMA removal efficiency by the plant. Since concentrations of IAA production by individual bacterium were highly correlated with TMA removal efficiency by plants in early periods of fumigation and highly correlated with leaf IAA production of bacterially inoculated plants, two predicted mechanisms on improving TMA uptake by bacterially inoculated plants are presented: (1) bacteria migration from plant roots to leaves increases leaf IAA concentration and (2) increasing concentration of bacterially inoculated root IAA inhibits transportation of IAA from leaves to roots, resulting in higher leaf IAA concentration. The higher concentration of leaf IAA is suggested to be a factor to increase stomatal opening which improves TMA removal efficiency of the plant.
Reduction of Cadmium Uptake of Rice Plants Using Soil Amendments in High Cadmium Contaminated Soil: A Pot Experiment Siswanto, Dian; Suksabye, Parinda; Thiravetyan, Paitip
Journal of Tropical Life Science Vol 3, No 2 (2013)
Publisher : Journal of Tropical Life Science

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Abstract

The aims of this study were to investigate the effect of agricultural residues on reducing cadmium uptake in rice plants. The rice plants growing on no cadmium/free cadmium soils (N), Cd soils (Cds), and Cd soils each amended with 1% w/w of coir pith (CP), coir pith modified with sodium hydroxide (CPm) and corncob (CC) under high cadmium contaminated soil with an average 145 mg Cd kg-1 soil were investigated. The results showed that the cumulative transpiration of rice grown in various treatments under high cadmium contaminated soil followed the order: Cds > CPm ≥ CP ≥ CC. These transpirations directly influenced cadmium accumulation in shoots and husks of rice plants. The CC and CP seemed to work to reduce the cadmium uptake by rice plants indicated by accumulated cadmium in the husk that were 2.47 and 7.38 mg Cd kg-1 dry weight, respectively. Overall, transpiration tended to drive cadmium accumulation in plants for rice grown in high cadmium contaminated soil. The more that plants uptake cadmium, the lower cadmium that remains in the soil. Keywords: Transpiration, Cadmium Uptake, Rice plant, Agricultural Residues
Allergenity Test of Porang Bulb Variant (Amorphophallus oncophyllus Hook.) By ELISA Ig-E Method Sofy Permana; Sri Widyarti; Serafinah Indriyani; Dian Siswanto; Aris Soewondo
Natural B, Journal of Health and Environmental Sciences Vol 1, No 4 (2012)
Publisher : Natural B, Journal of Health and Environmental Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (70.475 KB) | DOI: 10.21776/ub.natural-b.2012.001.04.14

Abstract

Porang (Amorphophalus oncophyllus Hook.), a tuber plant, is intensively explored for export commodities from East Java and as a raw food stuff material for domestic industry. The prospects of Porang tubers as the healthy food is interesting, however some studies are needed for their allergy effects. It was thought that the allergy effects of Porang are caused by the composition of Calcium Oxalate crystal that leads the itching and irritation in the mouth. On the other hands, the food allergy is usually caused by unchanged protein during food digesting.  Some proteins have been known that play a role in Calcium Oxalate crystal (Matrix proteins) formation, they are: Asp-rich and Ser-rich acidic proteins that are tightly bound to Calcium. So, substantially the allergen of Porang tuber is from the matrix protein, not from its crystal. The allergen in the food is usually a protein that is not change during the food digestion. In this study, it has been done the allergic test of some Porang tuber variants, namely: Sumber Baru-Jember; Klangon, Saradan-Madiun and Sumber Bendo, Saradan-Madiun, by ELISA-IgE from rat’s blood serum that have been orally treated by raw Porang tubers powder before.  The SDS-PAGE analysis shows that the proteins bands profiles are similar between raw and steamed Porang tubers (appearance Molecular Weight: 18 and 15 kDa). But, those raw Porang tubers bands are thicker than the steamed ones. The thickness of those raw Porang tubers decrease from 25 to 50 %, compare to those steamed ones. It means that the steaming of Porang is effective to decrease the potential allergy of Porang for consumptions. The allergic test analysis by ELISA-IgE revealed that the potential allergy of Porang are: Sumber Baru (Jember) > Porang var. Klangon - Saradan (Madiun) > Porang var. Sumber Bendo - Saradan (Madiun), respectively.
Virtual prediction of purple rice ferulic acid as anti-inflammatory of TNF-α signaling Ernanin Dyah Wijayanti; Anna Safitri; Dian Siswanto; Fatchiyah Fatchiyah
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 27 No 2 (2022): June 2022
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23869/bphjbr.27.2.20221

Abstract

Purple rice is one of the main sources of ferulic acid (FA). Some studies reported anti-inflammatory properties of FA, but the interaction of FA with TNF-α signaling has not been elucidated. TNF-α is a target for anti-inflammatory drug research due to its major role in the inflammatory process. This study aims to investigate the interaction of FA with TNF-α and TNF-α receptor (TNFR) through in silico study and evaluate the drug-like properties and biological activity of FA. The interactions among FA (CID 445858), TNF-α (2AZ5), and TNFR (1NCF) were docked by Hex 8.0.0 Cuda, then visualized by Discovery Studio 2020 and LigPlot V.1.4.5. Apigenin-7-glucuronide (AG, CID 5319484) was used as the positive control. The drug-like properties were predicted by Lipinski’s rule of five and the biological activity was analyzed by PASS online. FA showed good properties as a drug-like molecule and biological activity as an anti-inflammatory. FA also showed good interaction with TNF-α and TNFR. FA bound to TNF-α at Asn92(B), Val91(B), Leu93(B), Phe124(B), Phe124(D), and Leu93(D) residues with docking energy of -214.6 kJ/mol, and bound to TNFR at Pro16(A), Glu56(B), Cys55(B), Glu54(B) residues with docking energy of -191.1 kJ/mol. FA could inhibit TNF-α – TNFR interaction by binding to TNFR at Glu54 residue, the same inhibition mechanism to AG which bind to TNFR at Glu54 and Val90. The current study shows that FA has the potential as an anti-inflammatory of TNF-α signaling and can be developed as an oral anti-inflammatory drug candidate.
Komunitas Kapang Tanah di Lahan Kritis Berkapur DAS Brantas Pada Musim Kemarau Dian Siswanto; Suharjono Suharjono
Bioscientiae Vol 3, No 1 (2006): Bioscientiae Volume 3 No 1
Publisher : Universitas Lambung Mangkurat

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (110.701 KB) | DOI: 10.20527/b.v3i1.147

Abstract

The lime upland of Brantas River Watershed in East Java Province involve Malang, Blitar, Tulungagung and Trenggalek Regency are dry upland with limestone, low soil organic matter and unproductive. Soil productivity was influenced by microorganism activity that transform soil organic matter and other soil nutrition materials. Soil mold in lime upland of Brantas River Watershed can be used as bioindicator of productivity and soil sustainability. The research aims were to find out soil mold community at dry season in lime upland of The Brantas River Watershed and to know their relation with environmental factors. Chemical and physical parameters observed were soil temperature, sunlight intensity, soil acidity, soil moisture, soil organic matter and water retention capacity, while biological parameters was soil mold abundance.  The result showed that soil mold abundances between groups were significantly different. The range of soil mold abundances in Trenggalek were 7x103-2.4x104 propaguls/gram, Tulungagung were 1.8x103-1.0x104 propaguls/gram, Blitar were 7.8x102-4.6x103 propaguls/gram and Malang were 2.8x102-9.0x103 propaguls/gram. Soil mold abundances were not significantly different between village in Malang Regency contain Pagak Village were 2.27x104 propaguls/gram, in Ngembul Village were 7.91x103 propaguls/gram and Banyuurip Village were 3.32x103 propaguls/gram. Soil mold abundances between regency and between village both were not significantly influenced for chemical physic factors. Predominant soil mold in Trenggalek and Blitar were Penicillium while Mucor were dominant in Tulungagung, Penicillium and Phytophtora were codominant in Malang.
Antimicrobial Activity of Ferulic Acid in Indonesian Purple Rice through Toll-like Receptor Signaling Wijayanti, Ernanin Dyah; Safitri, Anna; Siswanto, Dian; Triprisila, Lidwina Faraline; Fatchiyah, Fatchiyah
Makara Journal of Science Vol. 25, No. 4
Publisher : UI Scholars Hub

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Purple rice is a potential source of ferulic acid, which has antimicrobial properties. However, the inhibitory mechanism of ferulic acid on the growth of bacteria, particularly Salmonella and Listeria, has not been elucidated. This study aimed to determine the bioactivity of ferulic acid from purple rice as an antimicrobial agent against Salmonella typhimurium and Listeria monocytogenes using in vitro and in silico analyses. The antimicrobial activity of a purple rice ferulic acid extract was tested using the agar well diffusion method. Its effect on bacterial cells was observed using scanning electron microscopy. Ferulic acid was confirmed to have antimicrobial properties using in silico software to attenuate the binding of bacterial virulence factors (lipoproteins, lipopolysaccharides, and flagellins) to Toll-like receptors (TLRs) and to prevent interactions with peptidoglycans. The purple rice ferulic acid extract inhibited bacterial growth. The inhibitory effects included induction of a biofilm and shrinkage of S. Typhimurium, as well as osmotic lysis of L. monocytogenes. This activity was supported by the ability of ferulic acid to inhibit the binding of bacterial virulence factors with TLRs and block bacterial peptidoglycans. These findings indicate that the purple rice ferulic acid extract acts as antimicrobial, both directly to bacterial cells and indirectly through TLRs. We conclude that ferulic acid from Indonesian purple rice has a biological function as an antimicrobial agent.
The Effectiveness of Various Types of Local Hydromacrophytes on The Phytoremediation Process of Catfish Pond Wastewater using a Batch Culture System : The Effectiveness of Various Types of Local Hydromacrophytes Umi Sa'adah; Dian Siswanto; Catur Retnaningdyah
Journal of Tropical Life Science Vol. 13 No. 1 (2023)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.13.01.02

Abstract

This study aimed to determine the effectivity of various types of local hydromacrophytes on the remediation of wastewater from catfish culture ponds in Gondosuli village, Gondang, Tulungagung, East Java, Indonesia, using a batch culture system. This experiment used a completely random design and was conducted in the glasshouse. The batch culture system was conducted in a 30 L tank containing sand and gravel as the medium for hydromacrophytes. This research consisted of five treatments (emergent, submerged, floating leaf, polyculture of 3 types of hydromacrophytes, and control without hydromacrophytes). This study was repeated three times at the same time. The effectiveness of the phytoremediation model was monitored using indicators of water physicochemical parameters and the biotic index of phytoplankton. The biotic indices were used the Shannon-Wiener diversity index (H') of phytoplankton and the diatom biotic index (Trophic Diatom Index (TDI) and Percentage of Pollution Tolerant Value (%PTV)). After giving the treatment, monitoring was carried out on days 0, 10, 20, 30, and 40. The results showed that the batch culture system for phytoremediation post-harvest catfish pond wastewater with local hydromacrophytes improved the water quality. However, not all water physicochemical parameters after treatments meet Indonesian water quality standards. Abundance showed water quality fluctuations over time, while Shannon Wiener's diversity index (H' value) decreased as water quality decreased. This study's PTV value demonstrated that pollutant levels vary by treatment. Only the control and submerged hydromacrophytes improved their TDI status; another treatment remained moderate. The best treatment was 40 days after acclimatization with polyculture treatment of three types of local hydromacrophytes. Research and other systems like continuous culture are needed for optimal results to improve water quality.
Biosistem Pertanian Apel Lokal Malang Dian Siswanto; Irfan Mustafa; Gustini Ekowati; Muhamad Imam; Endri Purnomo
The Journal of Experimental Life Science Vol. 1 No. 2 (2011)
Publisher : Postgraduate School, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jels.2011.001.02.06

Abstract

Biosistem yang digambarkan pada hasil observasi di sini adalah suatu keadaan faktor biotik dan abiotik pada pertanian apel yang tidak terbatas pada suatu nilai yang tetap. Data  yang diperoleh dari tiga tahapan penelitian ini diharapkan dapat dipergunakan sebagai informasi yang mendukung upaya konsevasi pertanian apel. Tahapan penelitian tersebut meliputi evaluasi nutrisi makro tanah dan tanaman, formulasi pengomposan kotoran sapi diperkaya (menggunakan bakteri indigenus) dan eksplorasi tanaman penutup tanah pertanian apel. Kandungan beberapa nutrisi makro untuk tanah pertanian apel Bumiaji menunjukkan nilai rata-rata yang berada pada kisaran sedang dan tinggi. Hasil analisis makro nutrisi daun apel yang kemudian dibandingkan dengan standar menurut Utah Fertilizer Guide menunjukkan bahwa makro nutrisi N, P, Ca berada pada status normal, K berada pada status kurang dan Mg berlebih. Ketersediaan nutrisi di tanah dapat dipelihara dengan penambahan kompos. Kotoran sapi yang diperkaya dengan bakteri indigen mampu mendekomposisi kotoran sapi dalam waktu 2-3 minggu. Proses dekomposisi kotoran sapi berlangsung lebih cepat pada pemberian bakteri indigenus yang mempunyai kemampuan selulolitik dan sekaligus proteolitik serta amilolitik apabila dibandingkan kontrol yang tanpa pemberian inokulum bakteri. Pada pertanian apel di Poncokusumo, kondisi lahan dengan sistem penyiangan cs (cukup sering) dan sj (sangat jarang) tampak lebih stabil daripada ss (sering sekali) berdasarkan nilai ID (indek diversitas), DR (dominansi relatif) dan INP (indek nilai penting). Hal ini diperjelas oleh nilai biomassa tanaman. Biomassa yang lebih tinggi menyebabkan suhu tanah yang lebih rendah karena jumlah TPT yang banyak mempunyai konsekuensi mengandung banyak air pada jaringannya. Kata kunci: Apel, nutrisi makro, kompos diperkaya, tanaman penutup tanah
The Sensitivity of Leersia hexandra Sw. to Gamma-Ray Irradiation Pritha Kartika Sukmasari; Wahyu Widoretno; Dian Siswanto
The Journal of Experimental Life Science Vol. 11 No. 1 (2021)
Publisher : Postgraduate School, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jels.2021.011.01.02

Abstract

Gamma-ray irradiation as a physical mutagen has high penetrating power. Therefore, it is most often used to increase genetic variability or produce new mutant plants. This research was conducted to obtain the lethal dose of gamma-rays in Leersia hexandra plants. The used plant part was a single node stolon which had a length of 10 cm with the node in the middle of the stolon. The irradiation doses given were 0, 25, 50, 75, 100 Gy. Stolons were inserted into plastic clips and irradiated using a Cobalt-60 gamma irradiation source at the Center for Irradiation and Radioisotope Applications (PAIR), National Nuclear Energy Agency (BATAN) Jakarta. Gamma-irradiation has a significant effect on inhibiting shoot growth. The growth of irradiated Leersia hexandra with the best number of plants and the highest shoots was obtained at a dose of 25 Gy and decreased with increasing irradiation dose. The lethal dose (LD50) was determined eight days after irradiation using CurveExpert 1.4 software. Leersia hexandra plants that can sprout and regenerate followed the linear equation y = 1.02 - 7.5x with LD50 at 68.85 Gy and LD20 at 29.36 Gy.Keywords: gamma irradiation, genetic variation, Leersia hexandra, lethal dose, mutant plant.
Co-Authors 'Ainun Sayyidah Zakiyah Aris Soewondo Catur Retnaningdyah Endri Purnomo Ernanin Dyah Wijayanti Estri Laras Arumingtyas Fatchiyah Fatchiyah Fatchiyah Fatchiyah Gustini Ekowati Irfan Mustafa Lidwina Faraline Triprisila Luchman Hakim Makagoshi, Nobukazu Muhamad Imam Pritha Kartika Sukmasari Rosina Monica Safitri, Anna Serafinah Indriyani Sofy Permana Sri Widyarti Suharjono Suharjono Suksabye, Parinda Thiravetyan, Paitip Tri Ardyati Umi Sa'adah Uswatun Hasanah Wahyu Widoretno