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Acute Toxicity Test Of The Green Viper Snake (Trimeresurus albolabris), Macroscopic Description Of The Kidney And Liver Of Mice (Mus musculus) Moch. Rizky Darmawan; Dadik Rahardjo; Wiwiek Tyasningsih; Rochmah Kurnijasanti; Djoko Legowo; Boedi Setiawan
Journal of Basic Medical Veterinary Vol. 10 No. 2 (2021): Journal of Basic Medical Veterinary, December 2021
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jbmv.v10i2.31135

Abstract

According to WHO (2010), the Trimeresurus albolabris snake is a venomous snake with a high number of bite cases in Indonesia. Information on the character and level of toxicity of snake venom in Indonesia, including Trimeresurus spp. snakes, is still minimal. This study aims to determine the LD50 value (lethal dose 50), clinical symptoms, and to determine the macroscopic appearance of the liver and kidneys of mice (Mus musculus) after being injected by a the white-lipped pit viper (Trimeresurus albolabris) venom. The LD50 value of Trimeresurus albolabris venom injected subcutaneously into mice was 5,09 mg/kgBW. Clinical symptoms that appear increased movement activity, increased respiratory rate, lethargy, decreased activity, and reduced grooming. Macroscopic appearance of the liver that looks abnormal is found (1) color changes in the form of pale, pale yellowish, and red spots (petechiae). (2) changes in surface structure found only one liver that is not flat. (3) changes in consistency in the form of brittle easily. In the kidney that looks abnormal, found (1) changes in color in the form of pale. (2) changes in surface structure were only found in one kidney in the form of nodules. (3) changes in consistency in the form of brittle easily and soft.
Identification and Prevalence of Blood Protozoa on Culling Layer Hen That Were Sold in Wonokromo Traditional Market Surabaya City Moch. Rizky Darmawan; Poedji Hastutiek; Arimbi Arimbi; Sunaryo Hadi Warsito; Dian Ayu Permatasari; Ririn Ririn
Journal of Parasite Science (JoPS) Vol. 6 No. 1 (2022): Journal of Parasite Science
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (460.274 KB) | DOI: 10.20473/jops.v6i1.34199

Abstract

The most common types of blood protozoa in chickens are from the phylum of Apicomplexa, including Plasmodium, Leucocytozoon and Haemoproteus. Blood protozoan diseases can cause decreased egg production, stunting and death in poultry farms. This study aims to identify and calculate the prevalence of blood protozoan infection in casted off laying chickens sold at Wonokromo Market, Surabaya City. One hundred blood samples from casted off laying chickens were taken by purposive sampling method. Blood samples were collected in Ethylene Diamine Tetra Acetate (EDTA) tubes, and then taken to the Parasitology Laboratory to make a blood smear which was then examined using a microscope with a magnification of 1000x. The results showed that the single infection was 43% positive for Plasmodium sp., 3% positive for Leucocytozoon sp. and 3% for mixed infections (Plasmodium sp. and Leucocytozoon sp.), with a total prevalence of positive samples infected with blood protozoa was 49%.