Poedji Hastutiek
Division Of Veterinary Parasitology, Faculty Of Veterinary Medicine, Universitas Airlangga

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Kerusakan Usus pada Mencit (Mus musculus) yang Diinokulasi Larva 3 (L3) Anisakis spp. Febrina Dian Permatasari; Poedji Hastutiek; Lucia Tri Suwanti
Jurnal Sain Veteriner Vol 35, No 1 (2017): Juni
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1467.385 KB) | DOI: 10.22146/jsv.29292

Abstract

This study aims to be show damage in the intestine of mice caused by inoculation of the larvae of Anisakis spp. alive and who has died because of a warming 750C for 7 minutes. Eighteen male mice (Mus musculus) were divided into three groups: control group, a group of mice were inoculated the larvae of Anisakis spp. alive andgroups of mice were inoculated the larvae of Anisakis spp. who have died. 48 hours post inoculation, the mice’s intestines necropsy performed later performed HE staining to identify and scoring intestinal histopathology. The results showed inoculation of the larvae of Anisakis spp. either alive or dead induce histological changes in the intestine in the form of infiltration of inflammatory cells, epithelial changes and structural changes in the intestinal mucosa
POTENSI Musca domesticaLinn. SEBAGAI VEKTOR BEBERAPA PENYAKIT Hastutiek, Poedji; Fitri, Loeki Enggar
Jurnal Kedokteran Brawijaya Vol 23, No 3 (2007)
Publisher : Fakultas Kedokteran Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1524.819 KB) | DOI: 10.21776/ub.jkb.2007.023.03.4

Abstract

Musca domestica is a fly which can be found aroundhouse and cage. Its present interference human hygiene, calmness and health. This fly generating many loss at human being and animal because this fly have high potency  in spreading many diseases agents, that are protozoa, worm, virus, bacterium and fungi. Several agents of emerging,reemerging and new emerging diseases can be transferred by M. domestica such as Cryptosporidium parvum, Helicobacter pylori, E.coli O157:H7 and H5N1. Agens removed by M. domestica through regurgitasi, excrete and exoskleleton. Interestingly M. domestica might be act as biological vector. Key words: Musca domestica, agent of disease
Identification of ectoparasites and endoparasites on fruit bats (Cynopterus brachyotis) in Ketapang Timur Village, Ketapang Sub-District, Sampang District Mohamad Safri Sauqi; Tjuk Imam Restiadi; Setiawan Koesdarto; Poedji Hastutiek; Boedi Setiawan; Agus Wijaya
Journal of Parasite Science (JoPS) Vol. 5 No. 2 (2021): Journal of Parasite Science
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (646.381 KB) | DOI: 10.20473/jops.v5i2.30365

Abstract

This study aims to determine the ectoparasites and endoparasites that infest fruit bats (Cynopterus brachyotis) in Ketapang Timur, Ketapang District, Sampang Regency. The samples in this study were blood, feces and ectoparasites from 50 fruit bats taken in Ketapang District, Sampang Regency. Blood samples were examined using a blood smear method with Giemsa staining, while stool samples were examined using three methods, namely native, sucrose floating, and acid-fast modification. Blood examination was checked using a microscope with 1000x magnification and stool examination at 400x magnification. The sample is considered positive if under microscope observation found parasites that match the characteristics of the protozoa sourced from scientific references. The results of the study of 50 fruit bats examined found five positives for digestive protozoa and ectoparasites, the infection was single, with details of one tail being infected with Eimeria sp. and one tail was infected by Leptocyclopodia ferrarii, while blood protozoa were not found. This study concludes that the type of protozoa found in the digestive tract is Eimeria sp. (14%) and ectoparasite Leptocyclopodia ferrarii. (2%). The total percentage obtained was 16% positive for a single infection of protozoa and ectoparasites from 50 fruit bats. Suggestions that can be put forward are to conduct further research using PCR and sequencing to obtain more specific and accurate identification results, for subspecies or strains.
Detection of Goat Digestive Tract Protozoa Through Feces Examination in Kwanyar Sub-District, Bangkalan District Meyreta Doti Alcaterana; Poedji Hastutiek; Nunuk Dyah Retno Lastuti; Endang Suprihati; Agus Sunarso
Journal of Parasite Science (JoPS) Vol. 5 No. 2 (2021): Journal of Parasite Science
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (467.145 KB) | DOI: 10.20473/jops.v5i2.30366

Abstract

This study aims to determine the prevalence and species of protozoa that infect the digestive tract of goats in Kwanyar Sub-District, Bangkalan District. This study was conducted in June-July 2019 with 100 samples of stool examination in the laboratory of the Division of Parasitology, Faculty of Veterinary Medicine, Airlangga University. On examination found two types of protozoa, which were: Eimeria sp and Blastocystis sp. The results of this study showed the prevalence of 48% digestive tract protozoa. The results of statistical analysis using the Chi-Square test showed significant differences in age under one year and over one year in goats (p <0.05).
Utilization of Curcuma Molases Block to Increase The Performance of Goats that Infected Gastrointestinal Worm at Wonorejo, Wates District, Kediri Regency Hani Plumeriastuti; Poedji Hastutiek; Lucia Tri Suwanti; Wiwik Misaco Yuniarti; Nusdianto Triakoso; Arimbi Arimbi
Journal of Parasite Science (JoPS) Vol. 2 No. 2 (2018): Journal of Parasite Science
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (461.314 KB) | DOI: 10.20473/jops.v2i2.16407

Abstract

This was a Community Service activity that aims to share knowladge about utilization of Curcuma Molasses Block (CMB) to increase performance of goats that infected gastrointestinal (GI) parasites to The Goat Breeder Group at  Wonorejo, Wates District, Kediri Regency. The activities include: counseling about raising goat management, presentasion on worm diseases that infect goats and CMB as anthelmentic, workshop and practice to make CMB, detection egg worm in goat`s feces, treatment on infected goats and filling out questionnaires evaluating the performance of goat before and after giving CMB. The result show that all of feces samples were positive contain egg worms of  Haemonchus contortus, Chabertina ovina, Trichuris globulosa, Strongyloides papillosus, Fasciola gigantica, Trichostrongylus spp., Toxocara sp., and Oesohagustomum columbianum even also protozoans  Eimeria sp., and Blastocystis sp. Administration CMB increase performance of worm-infected goats which indicated by increase in appetite, soft and shiny hair and the body more well-fed.
The Conformity of Diagnostic Test Between Burrow Ink Test Method With Skin Scrapping Method of Scabies in Rabbit (Lepus domesticus) Fadila Zikri Amanda; Poedji Hastutiek; Emy Koestanti Sabdoningrum; Nunuk Dyah Retno Lastuti; Endang Suprihati; Hana Eliyani
Journal of Parasite Science (JoPS) Vol. 2 No. 1 (2018): Journal of Parasite Science
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (532.383 KB) | DOI: 10.20473/jops.v2i1.16381

Abstract

This study intend to know the conformity of diagnostic test between burrow ink test method with skin scrapping method of scabies in rabbit. Scabies caused by Sarcoptes scabiei which has a predilection in stratum corneum and lucidum. Clinical symptoms was characterized by papula or crusting of the skin. This study presents a laboratory explorative research with cross sectional approach. Samples used 20 rabbits that infested scabies naturally. Data obtained by performing diagnostic burrow ink test method and skin scrapping in rabbits which are infested scabies naturally. KAPPA test used as data analysis. Results showed that the number of positive results based on burrow ink test method are 12 rabbits and 8 rabbits are negative while skin scrapping obtained the positive results are 11 rabbits and 9 rabbits are negative. KAPPA test analysis performed on the overall study data was obtained 0,490. The conclusion of this study is burrow ink test method has a good conformity with the skin scrapping in rabbits which are infested scabies naturally that can be used for diagnostic analysis of scabies with a simple method, faster, and have the same ability with skin scrapping without hurting animals.
The Prevalence and Infection’s Degree of Gastrointestinal Worm of Local Chicken (Gallus domesticus) in Kramat Village, District of Bangkalan, Madura, East Java Indonesia Ellza Agatha Damayanti; Poedji Hastutiek; Agnes Theresia Soelih Estoepangestie; Nunuk Dyah Retno Lastuti; Kusnoto Kusnoto; Endang Suprihati
Journal of Parasite Science (JoPS) Vol. 3 No. 1 (2019): Journal of Parasite Science
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (203.378 KB) | DOI: 10.20473/jops.v3i1.16436

Abstract

The study was aimed to know the prevalence and level of gastrointestinal worm investation of local chicken (Gallus domesticus) in Kramat Village, district of Bangkalan, Madura East Java province. The samples used in the study were 100 faeces of kampung chicken. using sedimentation and floating method. Faeces samples were tested, than followed by microscopically test to identify the worm is species. The result showed that in 38 out of 100 samples could be indentified some worm species as followed : Capillaria sp.14 (36,8%), Railletina 7 (18,4%), Hymenolepis 16 (42,1%) and Heterakis gallinarum 1 (2,6%). Using Mann Whitney U-test could be differentiated the level of the infection with an average of 45.53 in cock, while in hen with an average of 55.47. There was no significancy (p>0,05) on the level of worm gastrointestinal infection of different sex of kampung chicken.
Infestation Pattern of Lice In Laying Ducks In Village of Kramat District of Bangkalan Region of Bangkalan Kiki Amalia Rama; Poedji Hastutiek; Oky Setyo Widodo; Endang Suprihati; Agus Sunarso; Soeharsono Soeharsono
Journal of Parasite Science (JoPS) Vol. 1 No. 2 (2017): Journal of Parasite Science
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (414.959 KB) | DOI: 10.20473/jops.v1i2.16286

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The purpose of this research is to identify lice and to know the pattern of infestation of lice that infest laying ducks in Village of Kramat, District  of Bangkalan, Region of Bangkalan. Forty samples of laying ducks were taken in Kramat Village and identified in Entomology and Protozoology Laboratory of Parasitology Department of Veterinary Faculty of Airlangga University, the research was conducted from July to September 2017. This study used an explorative observational research design. Lice taken from a sample of laying ducks are fed into an ointment pot containing 70% alcohol to preserve lice and labeled in accordance with the infected duck's limb region. Identification of lice using Permanent mounting method without coloration. The results of identification, the lice that infest laying ducks consists of three types of Anaticola crassicornis 60%, Menacanthus stramineus 25% and Lipeurus caponis 17.5%. The results of infestation pattern, infected body's region is head-neck, wings and back. The wings infested two types of lice were A. crassicornis and L. caponis, head-neck infested M. stramineus and in the infected back region of A. crassicornis.
The Effectiveness of Ethanol Extract of Red Betel Leaf (Piper crocatum) Againts Mortality of Boophilus microplus Larvae in Vitro Meta Aprilia; Poedji Hastutiek; Rochmah Kurnijasanti; Lucia Tri Suwanti; Mohammad Sukmanadi; Endang Suprihati
Journal of Parasite Science (JoPS) Vol. 3 No. 1 (2019): Journal of Parasite Science
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (151.694 KB) | DOI: 10.20473/jops.v3i1.16429

Abstract

The aims of this study is to know the effectiveness of ethanol extract of red betel leaf (Piper crocatum) against mortality of Boophilus microplus Larvae in Vitro, as well as knowing lethal concentration 50 (LC50) and lethal concentration 90 (LC90). Method that used in the research was completely randomized design. There were five treatments and each treatment was done in five replications. This research used ten Boophilus microplus larvae in each treatment for all replication. The observation and recording of mortality was done at 1, 2, 3, 4, and 5 hours. Boophilus microplus larvae were declared dead if there was no movement. The obtained data was analyzed using ANOVA factorial. The result of this research show that ethanol extract of red betel leaf (Piper crocatum) has effectiveness against mortality of Boophlius microplus larvae in Vitro. In the extract 10% concentration, there is effectiveness that almost the same as Noticks®. The higher concentration of the extract, the higher the effectiveness against mortality lavae.In probit analysis show that LC50 achieved by concentration 5,4% or 2.700mg/50ml and LC90 achieved by concentration 11,3% or 5.650mg/50ml.
Identification and Distribution of Soil Transmitted Helminths around The Shed and Grazing Fields of Madura Cattle in Sub-District of Geger, Bangkalan Regency Dewi Mahartina; Poedji Hastutiek; Sri Mulyati; Soeharsono Soeharsono; Muhammad Hambal
Journal of Parasite Science (JoPS) Vol. 4 No. 2 (2020): Journal of Parasite Science
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (454.979 KB) | DOI: 10.20473/jops.v4i2.29153

Abstract

This research aims to determine the percentage and kind of Soil Transmitted Helminths contamination in around the shed and grazing field soil of Madura cattle in Sub-District of Geger, Bangkalan District. The research was conducted on July until September 2018. The method of this research used a non-experimental method and through an observation study. The sample of this study is 100 samples of around the shed and grazing field soil, then examined in the laboratory of Helminthology, Division of Parasitology, Faculty of Veterinary Medicine, Universitas Airlangga used Modifed Sucrose 58%. Based on the kind of contamination, the highest contamination was Toxocara sp. (45.9%). Followed by Strongyloides sp. (41.4%), Trichuris sp. (9.5%) and Ancylostoma sp. (3.2%). Based on location, the percentage of grazing fields was higher (70%) than around the shed (50%). The result of statistical analysis using Chi- Square test showed significant differences in the percentage between around the shed and grazing field soil (p<0.05).
Co-Authors Abdul Samik Aditya Yudhana Agnes Theresia Soelih Estoepangestie Agus Sunarso Agus Sunarso Agus Wijaya Aldi Hamdani Amelia Dwita Safitri Amirotul Azhimah Amirul Muslim Amrullah Amung Logam Saputro Ana Amaliah Arimbi Arimbi Arimbi Arimbi Arum Puspitasari Benjamin Christoffel Tehupuring Bodhi Agustono Boedi Setiawan Boedi Setiawan Budiarto Budiarto Cahaya Panjaitan David Mohamad Qadafi Dewi Mahartina Dhandy Koesoemo Wardhana Dhimar Maulud Dyahningrum Dian Ayu Permatasari Diyah Ayu Candra Ellza Agatha Damayanti Elok Apriliawati Emy Koestanti Sabdoningrum Endang Suprihati Fadila Zikri Amanda Fania Selfiannisa Febrina Dian Permatasari Fifi Anik Suroiyah Firda Shafa Salsabila Firman Hadi Fanani Ghifari Lutfi Fauzi Hana Eliyani Hani Plumeriastuti I Komang Wiarsa Sardjana Indah Norma Triana Intan Nurcahya Iwan Sahrial Hamid Iwan Sahrial Hamid Iwan Sahrial Hamid Jihaan Haajidah Kartika Aditiya Amelia Kiki Amalia Rama Kusnoto Kusnoto Lianny Nangoi Lilik Maslachah Lilik Maslachah Loeki Enggar Fitri Lucia Tri Suwanti, Lucia Tri M.Gandul Atik Yuliani Marchelia Arifiandani Melani Anggraini Meta Aprilia Meyreta Doti Alcaterana Moch. Rizky Darmawan Mochamad Lazuardi Moh. Ilham Rizkul Ulum Mohamad Safri Sauqi Mohammad Sukmanadi Muchammad Yunus Mufasirin . Muhammad Hambal Muhammad Hambal Muhammad Ridwan Muhammad Ridwan Muhammad Thohawi Elziyad Purnama NASHIHATUS SHOLIHAH Nenny Harijani Nenny Harijani Nila Murodah Nining Sari Virgandina Vinola Nizar Bachrudin Prihandono Nove Hidayati Nunuk Dyah Retno Lastuti Nur Sa’adah Sulaeman Nusdianto Triakoso Oky Setyo Widodo Prima Ayu Wibawati Primarizky, Hardany Rahaju Ernawati Rahmi Sugihartuti Ratih Novita Praja Ratna Damayanti Ratu Meidiza Retno Bijanti Retno Wulansari Ririn Ririn Rochmah Kurniasanti Rochmah Kurnijasanti Setiawan Koesdarto Sherbina Bai Soeharsono Soeharsono Soeharsono Soeharsono Sri Hidanah Sri Mulyati Sri Mumpuni Sosiawati Suherni Susilowati Sunarso, Agus Sunaryo Hadi Warsito Suryanie Sarudji Talita Yuanda Reksa Thomas Valentinus Widiyatno Tjuk Imam Restiadi Tri Wahyu Suprayogi Venti Safitri Warda Nafalizza Efendi Warsito, Sunaryo Hadi Wiwik Misaco Yuniarti Wurlina Wurlina Yeni Dhamayanti